Difference between revisions of "Team:BIT-China"

 
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<h1> Sweet Sensing Yeast </h1>
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<h2> Description </h2>
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<p>Low-caloric artificial sweeteners (LAS) and Low-caloric natural sweeteners (LNS) are among the most widely used food additives worldwide, regularly consumed by lean and obese individuals alike. With the improvement of people's living standard，desired sweeteners are expected far more eagerly due to the growing living level and the developing science technology. The goal of this work is to introduce the human sweet receptor T1R2-T1R3 system to create a practical application of a sweetener sensor using an engineered yeast strain. Initially，we replacing the endogenous yeast G-protein Ste2 with the human sweet receptor T1R2-T1R3, additionally, we deleted some genes to further improve the sensitivity of the T1R2-T1R3 reporter signal. And we also introduce a RFP expression cassette to characterized the relative sweetness degree by RFP fluorescence intensity. Our work will assist the development of a fluorescence-based - sweetening agent sensing system using engineered yeast.</p>
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<h2 class="title-h2">ABSTRACT</h2>
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      In a parallel galaxy, there were two brothers named T1R2 and T1R3, they lived in a galaxy that was under the threat of sweet monsters who wanted to rule the galaxy. Though the brothers were born with the ability to eat these monsters, they were too weak to fight with so many enemies. To be stronger, they asked the battle creatures who lived in planet JB486 for help and sent their genes to a battle king SC84117 in the meantime. Driven by a mysterious force from the depths of the universe, the battle king, after adopting the gene <i>T1R2/T1R3</i>, lost its genes <i>ste2</i>, <i>sst2</i> and <i>far1</i>. After integration with <i>T1R2/T1R3</i> perfectly, something magical happened, a spaceship with a red ruler appeared. The battle king transformed into this spaceship which can eat sweet monsters, known as the Sugar Hunter. Since then, he travels all around the galaxy to fight sweet monsters and use the red ruler to measure them, the galaxy stays peaceful ever after.</p>
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<p>GPCRs constitute the largest family of membrane proteins and are involved in the majority of signal transduction. The common architecture of GPCRs has a seven transmembrane domain structure (<i>Fredriksson et al.</i>, 2003; <i>Takeda et al.</i>, 2002) that responds to a remarkable range of stimuli, including neurotransmitters, hormones, and ions (<i>Lefkowitz and Shenoy</i>, 2005). G proteins are binary switches that are ‘off’ when bound to guanosine diphosphate (GDP) and ‘on’ when bound to guanosine triphosphate (GTP). The P-loop is responsible for the correct positioning of the triphosphate moiety of the bound nucleotide (<i>M. Saraste</i>,1990). Activation of the G protein stabilises the effector binding site via an interaction with a conserved threonine residue in switch I and a glycine residue in the switch II region, which form hydrogen bonds with the γ-phosphate of GTP in combination with a Mg<sup>2+</sup> cofactor. This functionality is conferred by an ∼20 kDa G domain that is conserved across all G proteins .
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Latest revision as of 12:24, 1 November 2017

BIT-CHINA

ABSTRACT

In a parallel galaxy, there were two brothers named T1R2 and T1R3, they lived in a galaxy that was under the threat of sweet monsters who wanted to rule the galaxy. Though the brothers were born with the ability to eat these monsters, they were too weak to fight with so many enemies. To be stronger, they asked the battle creatures who lived in planet JB486 for help and sent their genes to a battle king SC84117 in the meantime. Driven by a mysterious force from the depths of the universe, the battle king, after adopting the gene T1R2/T1R3, lost its genes ste2, sst2 and far1. After integration with T1R2/T1R3 perfectly, something magical happened, a spaceship with a red ruler appeared. The battle king transformed into this spaceship which can eat sweet monsters, known as the Sugar Hunter. Since then, he travels all around the galaxy to fight sweet monsters and use the red ruler to measure them, the galaxy stays peaceful ever after.


Contact

Institute of Biotransformation and synthetic biosystem

School of life science

Beijing Institute of Technology

100081, Beijing

Email: lichun@bit.edu.cn