Difference between revisions of "Team:NYMU-Taipei/Measurement"
(Replaced content with "{{NYMU-Taipei}} {{NYMU-Taipei-css}} <html> <head> </head> <!-- start of content --> <body> <div class = "igem_2017_content_wrapper"> </div> </body...") |
|||
| (3 intermediate revisions by one other user not shown) | |||
| Line 5: | Line 5: | ||
<head> | <head> | ||
| + | <style> | ||
| + | .igem_2017_content_wrapper b { | ||
| + | font-family:'Acme', sans-serif; | ||
| + | color:#3dba32; | ||
| + | } | ||
| + | .igem_2017_content_wrapper a { | ||
| + | color:#38af2f; | ||
| + | text-decoration-color:#38af2f; | ||
| + | } | ||
| + | .igem_2017_content_wrapper a:hover { | ||
| + | color:#f99595; | ||
| + | } | ||
| + | </style> | ||
</head> | </head> | ||
| Line 10: | Line 23: | ||
<body> | <body> | ||
<div class = "igem_2017_content_wrapper"> | <div class = "igem_2017_content_wrapper"> | ||
| − | + | <h1>Measurement</h1> | |
| + | |||
| + | <p> In our project, we used a <b>photo-bioreactor</b> for microbial cell growth measurement. The photo-bioreactor is originally used to determine gene expression during cell growth, but we used it to measure the growth curve during continuous cultivation in BG-11 of two microalgae in our project. | ||
| + | </p> | ||
| + | <br> | ||
| + | <p>The followings are the advantages of using this measurement method:</p> | ||
| + | <ol> | ||
| + | <li>Photo-bioreactor is built from Arduino microcontroller, which is an open source software and are relatively cheap in price. Thus, the photo-bioreactor we use is generally cheaper than regular photoreactors/spectrophotometers researchers use to measure absorbance. | ||
| + | <li>Compared to recording a single data every time with the use of spectrophotometer, photo-bioreactor can eliminate batch-to-batch variation after measuring several time points continuously, which significantly improves the accuracy of measurement. | ||
| + | <li>Once calibrated, photo-bioreactor can automatically cultivate microalgae, measure concentration over a long period of time, and restore data back to computer automatically. Thus, it can eliminate manual measurements and saves more time for researchers. | ||
| + | </ol> | ||
| + | <p>(See more detail: <a href="https://2017.igem.org/Team:NYMU-Taipei/Nitrogen_starvation">Nitrogen Starvation - Experiments - Automatic Measurement</a>)</p> | ||
| + | |||
</div> | </div> | ||
Latest revision as of 12:45, 1 November 2017
Measurement
In our project, we used a photo-bioreactor for microbial cell growth measurement. The photo-bioreactor is originally used to determine gene expression during cell growth, but we used it to measure the growth curve during continuous cultivation in BG-11 of two microalgae in our project.
The followings are the advantages of using this measurement method:
- Photo-bioreactor is built from Arduino microcontroller, which is an open source software and are relatively cheap in price. Thus, the photo-bioreactor we use is generally cheaper than regular photoreactors/spectrophotometers researchers use to measure absorbance.
- Compared to recording a single data every time with the use of spectrophotometer, photo-bioreactor can eliminate batch-to-batch variation after measuring several time points continuously, which significantly improves the accuracy of measurement.
- Once calibrated, photo-bioreactor can automatically cultivate microalgae, measure concentration over a long period of time, and restore data back to computer automatically. Thus, it can eliminate manual measurements and saves more time for researchers.
(See more detail: Nitrogen Starvation - Experiments - Automatic Measurement)
