Difference between revisions of "Team:NYMU-Taipei/Measurement"

 
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<p>The followings are the advantages of using this measurement method:</p>
 
<p>The followings are the advantages of using this measurement method:</p>
 
<ol>
 
<ol>
<li>Due to the opened command lines and cheapness of building Arduino devices, the photo-bioreactor we used is cheaper than general photoreactors or spectrophotometers for researchers to measure absorbance.
+
<li>Photo-bioreactor is built from Arduino microcontroller, which is an open source software and are relatively cheap in price. Thus, the photo-bioreactor we use is generally cheaper than regular photoreactors/spectrophotometers researchers use to measure absorbance.
<li>Compared to recording a single record every time by spectrophotometer, the photo-bioreactor can eliminate batch-to-batch variation after measuring several time points continuously, which significantly improves the accuracy of measurement.
+
<li>Compared to recording a single data every time with the use of spectrophotometer, photo-bioreactor can eliminate batch-to-batch variation after measuring several time points continuously, which significantly improves the accuracy of measurement.
<li>Once calibrated, the photo-bioreactor can automatically cultivate microalgae, measuring concentration in a long period of time, and restores data back to computer automatically. It can eliminate the long-term manual measurements and saves more time for researchers.
+
<li>Once calibrated, photo-bioreactor can automatically cultivate microalgae, measure concentration over a long period of time, and restore data back to computer automatically. Thus, it can eliminate manual measurements and saves more time for researchers.
 
</ol>
 
</ol>
 
<p>(See more detail: <a href="https://2017.igem.org/Team:NYMU-Taipei/Nitrogen_starvation">Nitrogen Starvation - Experiments - Automatic Measurement</a>)</p>
 
<p>(See more detail: <a href="https://2017.igem.org/Team:NYMU-Taipei/Nitrogen_starvation">Nitrogen Starvation - Experiments - Automatic Measurement</a>)</p>

Latest revision as of 12:45, 1 November 2017

Measurement

  In our project, we used a photo-bioreactor for microbial cell growth measurement. The photo-bioreactor is originally used to determine gene expression during cell growth, but we used it to measure the growth curve during continuous cultivation in BG-11 of two microalgae in our project.


The followings are the advantages of using this measurement method:

  1. Photo-bioreactor is built from Arduino microcontroller, which is an open source software and are relatively cheap in price. Thus, the photo-bioreactor we use is generally cheaper than regular photoreactors/spectrophotometers researchers use to measure absorbance.
  2. Compared to recording a single data every time with the use of spectrophotometer, photo-bioreactor can eliminate batch-to-batch variation after measuring several time points continuously, which significantly improves the accuracy of measurement.
  3. Once calibrated, photo-bioreactor can automatically cultivate microalgae, measure concentration over a long period of time, and restore data back to computer automatically. Thus, it can eliminate manual measurements and saves more time for researchers.

(See more detail: Nitrogen Starvation - Experiments - Automatic Measurement)