Difference between revisions of "Team:Sydney Australia/Basic Part"

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{{Sydney_Australia}}
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{{:Team:Sydney_Australia/Templates/NavBar}}
 
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<div class="column full_size judges-will-not-evaluate">
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Basic Parts</h1>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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</p>
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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.box5 {
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<br>
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<h3>Best Basic Part Special Prize</h3>
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  border-color: #93bcc1;
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are *many* opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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#please h1 {
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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<h4>Note</h4>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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<div class="container-fluid">
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<div class = "row" style="background-color:#e2e2e2;margin-top:60px;">
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      <img class="crispy center-block img-center" src="https://static.igem.org/mediawiki/2017/e/e4/T--Sydney_Australia--BasicPartsBanner.gif" width = "80%">
 
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<div class = "row">
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<div class = "content" id="please">
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<center><h1>BASIC PARTS</h1>
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<div class="box4"></div></center>
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<div class="row" style="height:2vw;"></div>
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</div>
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</div>
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<center><h4>Our basic parts are the fundamental bricks that make up our composite parts, and include regulatory regiongs such as an efficient ribosome binding site, tags for modifying production of proteins of interest, such as affinity chromatography tags and tags for a variety of translocations within various cell types. They also contain the primary protein coding regions that we studied in this project – Human Proinsulin <a href="http://parts.igem.org/Part:BBa_K2417006">(BBa_K2417006)</a>and Winsulin <a href="http://parts.igem.org/Part:BBa_K2417007">(BBa_K2417007)</a>.<br><br><br><br></h4>
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</center>
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</div>
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</div>
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<div class = "content" id="please">
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<div style="text-align:center">
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<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt; mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.4pt">
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  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
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  mso-border-alt:solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:
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  accent1;mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
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  font-family:Quicksand"><br>
 +
  PART NO.<o:p></o:p></span></b></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  </td>
 +
  <td width="144" valign="top" style="width:143.85pt;border:solid windowtext 1.0pt;
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 +
  solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:accent1;
 +
  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><br>
 +
  PART NAME<o:p></o:p></span></b></p>
 +
  </td>
 +
  <td width="116" valign="top" style="width:115.95pt;border:solid windowtext 1.0pt;
 +
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 +
  solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:accent1;
 +
  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><br>
 +
  SOURCE<o:p></o:p></span></b></p>
 +
  </td>
 +
  <td width="323" valign="top" style="width:322.55pt;border:solid windowtext 1.0pt;
 +
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 +
  solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:accent1;
 +
  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><br>
 +
  DESCRIPTION<o:p></o:p></span></b></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:1">
 +
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417004">BBa_K2417004</a><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Glycine Glycine Serine (x4) Linker Motif A<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Designed<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Linker motif used
 +
  to provide spacer region<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Used to expose
 +
  affinity purification tags such as 6x His tag<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Serine and glycine
 +
  used as they are small and flexible<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:2">
 +
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417006">BBa_K2417006</a><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human Proinsulin Coding Sequence<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Humans<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Coding sequence for
 +
  human proinsulin, sourced from NCIB<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Consists of A, B
 +
  and C chains, but C-peptide is cleaved out<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Contains 3 <span class="SpellE">disulphide</span> bonds<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Contains arginine
 +
  residues adjacent to C-peptide to allow cleavage<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:3">
 +
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417007">BBa_K2417007</a><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
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  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand">Winsulin - Single Chain Insulin Analogue<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Designed<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Consists of short linker peptide instead of C-peptide<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Does not require cleavage therefore simplifies production procedures<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Linker peptide raises <span class="SpellE">pI</span> of insulin analogue (long lasting after administration)<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Increased <span class="SpellE">thermostability</span> (based on other single chain insulin analogues tested)<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:4">
 +
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417008">BBa_K2417008</a><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"> 6x Poly-Histidine Tag codon optimized for use in <i style="mso-bidi-font-style:
 +
  normal">E. coli</i><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br> Designed (optimized for use in <i style="mso-bidi-font-style:normal">Escherichia coli</i>)<br style="mso-special-character:line-break">
 +
  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
 +
  <!--[endif]--><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l6 level1 lfo6"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Codon optimized for use in <i style="mso-bidi-font-style:normal">E. coli</i><o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l6 level1 lfo6"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Used to immobilize ions on a chromatography affinity column (nickel, cobalt or copper)<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l6 level1 lfo6"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Small, and easy to add to the ends of proteins of interest<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:5;height:27.45pt">
 +
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
 +
  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
 +
  <!--[endif]--><o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417009">BBa_K2417009</a><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br>Extended ribosome binding site<o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">T7 major capsid protein<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l3 level1 lfo7"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">High ribosomal recruitment efficiency<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l3 level1 lfo7"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Sourced from the pET15b vectors<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:6">
 +
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
 
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417010">BBa_K2417010</a><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-size:
 +
  14.0pt;font-family:Quicksand"> Periplasmic Signal Peptide<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 +
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 +
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Escherichia coli<o:p></o:p></span></i></p>
 +
  </td>
 +
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Small periplasmic, <span class="SpellE">homodimeric</span> protein<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Upon fusion to N-terminus of protein will translocate protein to periplasm of <i style="mso-bidi-font-style:normal">E. coli</i><o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Periplasm is an oxidative environment ideal for protein folding, and simplifies extraction due to eliminating the need for purifying inclusion bodies from the cytoplasm<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Periplasm has reduced proteases<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:7">
 +
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
 +
  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
 +
  <!--[endif]--><o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417011">BBa_K2417011</a><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand">YncM Bacillus Secretion Peptide<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 +
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 +
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Bacillus subtilis<o:p></o:p></span></i></p>
 +
  </td>
 +
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l0 level1 lfo9"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Can be fused to the
 +
  N-terminus of proteins to induce secretion to the surrounding media wheN proteins are expressed in <i style="mso-bidi-font-style:normal">Bacillus subtilis</i><o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l0 level1 lfo9"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Eliminates many
 +
  steps in the purification process<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:8;height:85.45pt">
 +
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417012">BBa_K2417012<o:p></o:p></a></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV Protease Cleavage Sequence<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A<o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l4 level1 lfo10"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Sequence for which TEV protease is specific to<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l4 level1 lfo10"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Can be added between protein of interest and peptides that require removal, such as purification tags<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:9;mso-yfti-lastrow:yes">
 +
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417013">BBa_K2417013</a><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Glycine
 +
  <span class="SpellE">Glycine</span> Serine (x4) Linker <o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Motif
 +
  B<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Designed<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Linker motif used
 +
  to provide spacer region<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Used to expose
 +
  affinity purification tags such as 6x His tag<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Serine and glycine
 +
  used as they are small and flexible<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
</div>
 +
</div>
 +
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Latest revision as of 13:53, 1 November 2017

BASIC PARTS

Our basic parts are the fundamental bricks that make up our composite parts, and include regulatory regiongs such as an efficient ribosome binding site, tags for modifying production of proteins of interest, such as affinity chromatography tags and tags for a variety of translocations within various cell types. They also contain the primary protein coding regions that we studied in this project – Human Proinsulin (BBa_K2417006)and Winsulin (BBa_K2417007).




PART NO.

 


PART NAME


SOURCE


DESCRIPTION

 

 

BBa_K2417004

 

Glycine Glycine Serine (x4) Linker Motif A

 

 

Designed

·      Linker motif used to provide spacer region

·      Used to expose affinity purification tags such as 6x His tag

·      Serine and glycine used as they are small and flexible

 

 

 

BBa_K2417006

 

 

Human Proinsulin Coding Sequence

 

 

 

Humans

·      Coding sequence for human proinsulin, sourced from NCIB

·      Consists of A, B and C chains, but C-peptide is cleaved out

·      Contains 3 disulphide bonds

·      Contains arginine residues adjacent to C-peptide to allow cleavage

 

 

BBa_K2417007

 

Winsulin - Single Chain Insulin Analogue

 

 

Designed

·      Consists of short linker peptide instead of C-peptide

·      Does not require cleavage therefore simplifies production procedures

·      Linker peptide raises pI of insulin analogue (long lasting after administration)

·      Increased thermostability (based on other single chain insulin analogues tested)

 

 

BBa_K2417008

 

6x Poly-Histidine Tag codon optimized for use in E. coli


Designed (optimized for use in Escherichia coli)

·      Codon optimized for use in E. coli

·      Used to immobilize ions on a chromatography affinity column (nickel, cobalt or copper)

·      Small, and easy to add to the ends of proteins of interest



BBa_K2417009


Extended ribosome binding site

 

 

T7 major capsid protein

·      High ribosomal recruitment efficiency

·      Sourced from the pET15b vectors

 

 

BBa_K2417010

 

 

Ecotin Periplasmic Signal Peptide

 

 

Escherichia coli

·      Small periplasmic, homodimeric protein

·      Upon fusion to N-terminus of protein will translocate protein to periplasm of E. coli

·      Periplasm is an oxidative environment ideal for protein folding, and simplifies extraction due to eliminating the need for purifying inclusion bodies from the cytoplasm

·      Periplasm has reduced proteases

 



BBa_K2417011

 

 

YncM Bacillus Secretion Peptide

 

 

Bacillus subtilis

·      Can be fused to the N-terminus of proteins to induce secretion to the surrounding media wheN proteins are expressed in Bacillus subtilis

·      Eliminates many steps in the purification process

 

 

BBa_K2417012

 

 

 

TEV Protease Cleavage Sequence

 

 

N/A

 

 

·      Sequence for which TEV protease is specific to

·      Can be added between protein of interest and peptides that require removal, such as purification tags

 

 

BBa_K2417013

 

Glycine Glycine Serine (x4) Linker

Motif B

 

 

Designed

·      Linker motif used to provide spacer region

·      Used to expose affinity purification tags such as 6x His tag

·      Serine and glycine used as they are small and flexible