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| <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script> | | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script> |
| <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet"> | | <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet"> |
− | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
| |
− | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
| |
| <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script> | | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script> |
| <link href="https://fonts.googleapis.com/css?family=Quicksand:300,400|Roboto:400,400i" rel="stylesheet"> | | <link href="https://fonts.googleapis.com/css?family=Quicksand:300,400|Roboto:400,400i" rel="stylesheet"> |
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| font-family: 'Quicksand', sans-serif; | | font-family: 'Quicksand', sans-serif; |
| color: #3e3f3f; | | color: #3e3f3f; |
− | font-size: 16px; | + | font-size: 20px; |
| LINE-HEIGHT:20px; | | LINE-HEIGHT:20px; |
| | | |
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| color: #3e3f3f; | | color: #3e3f3f; |
| font-size: 20px; | | font-size: 20px; |
| + | padding:40px; |
| | | |
| } | | } |
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| padding:0 10px 10px 10px; | | padding:0 10px 10px 10px; |
| max-height:2px; | | max-height:2px; |
− | min-width:800px; | + | width:500px; |
| } | | } |
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| | | |
| .box4 { | | .box4 { |
− | background:#fad990; | + | background:#D9E2F3; |
− | border-color: #fad990; | + | border-color: #D9E2F3; |
| } | | } |
| | | |
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| background:#93bcc1; | | background:#93bcc1; |
| border-color: #93bcc1; | | border-color: #93bcc1; |
| + | width:900px; |
| } | | } |
| | | |
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| </head> | | </head> |
| <body> | | <body> |
| + | <div class="container-fluid"> |
| | | |
− | | + | <div class = "row" style ="background-color:#e2e2e2;margin-top:60px;"> |
− | <div class = "bannerbox" style ="background-color:#e2e2e2"> | + | <img class="center-block img-center" src="https://static.igem.org/mediawiki/2017/4/48/T--Sydney_Australia--partspage_banner.png" width="80%"> |
− | <div class = "center">
| + | |
− | <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Sydney_Australia--PartsBanner.gif" width="70%">
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | | + | |
− | <div class="row" style="height:5vw;"></div>
| + | |
− | | + | |
− | <div class = "row">
| + | |
− | <div class = "content" id="please">
| + | |
− | <center><h1>GOLD</h1></center>
| + | |
− | <div class="box1"></div>
| + | |
− | <div class="row" style="height:2vw;"></div>
| + | |
− | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="99%" style="width:99.54%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| + | |
− | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| + | |
− | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.35pt">
| + | |
− | <td width="100%" colspan="4" style="width:100.0%;border:solid windowtext 1.0pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;background:#E6BC2E;padding:0cm 5.4pt 0cm 5.4pt;
| + | |
− | height:18.35pt">
| + | |
− | <p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:20.0pt;font-family:Quicksand">GOLD<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:1;height:19.05pt">
| + | |
− | <td width="19%" style="width:19.88%;border:solid windowtext 1.0pt;border-top:
| + | |
− | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| + | |
− | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="14%" style="width:14.62%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
| + | |
− | height:19.05pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TYPE<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="21%" style="width:21.14%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
| + | |
− | height:19.05pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">SOURCE<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="44%" style="width:44.36%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
| + | |
− | height:19.05pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">KEY FEATURES<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:2;height:78.9pt">
| + | |
− | <td width="19%" style="width:19.88%;border:solid windowtext 1.0pt;border-top:
| + | |
− | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| + | |
− | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">YncM-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">BBa_K2417005<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="14%" style="width:14.62%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="21%" style="width:21.14%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="44%" style="width:44.36%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| + | |
− | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpFirst" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel short chain
| + | |
− | insulin analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker
| + | |
− | (QRGGGSGGGQRR)<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"> secretion tag to
| + | |
− | translocate protein into media<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His
| + | |
− | Tag purification<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease
| + | |
− | cleavage site<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpLast" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome
| + | |
− | binding site<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" style="margin-left:18.0pt"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:3;height:78.9pt">
| + | |
− | <td width="19%" rowspan="2" style="width:19.88%;border:solid windowtext 1.0pt;
| + | |
− | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| + | |
− | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">BBa_K2417000<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="14%" rowspan="2" style="width:14.62%;border-top:none;border-left:
| + | |
− | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="21%" style="width:21.14%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| + | |
− | tag)<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="44%" rowspan="2" style="width:44.36%;border-top:none;border-left:
| + | |
− | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| + | |
− | <p class="MsoNormal" style="margin-left:18.0pt"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpFirst" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human proinsulin <o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span> tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His
| + | |
− | tag <o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GGS flexible
| + | |
− | linker<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">trypsin protease
| + | |
− | cleavage site for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosomal
| + | |
− | binding site <o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:4;height:72.85pt">
| + | |
− | <td width="21%" style="width:21.14%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| + | |
− | proinsulin)<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:5;mso-yfti-lastrow:yes;height:135.8pt">
| + | |
− | <td width="19%" style="width:19.88%;border:solid windowtext 1.0pt;border-top:
| + | |
− | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| + | |
− | padding:0cm 5.4pt 0cm 5.4pt;height:135.8pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes"> </span>BBa_K2417001<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="14%" style="width:14.62%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:135.8pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="21%" style="width:21.14%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:135.8pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| + | |
− | proinsulin)<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="44%" style="width:44.36%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:135.8pt">
| + | |
− | <p class="MsoListParagraphCxSpFirst" style="text-indent:-18.0pt;mso-list:l3 level1 lfo2"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l3 level1 lfo2"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His
| + | |
− | tag <o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l3 level1 lfo2"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l3 level1 lfo2"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Trypsin protease
| + | |
− | cleavage site for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpLast" style="text-indent:-18.0pt;mso-list:l3 level1 lfo2"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosomal
| + | |
− | binding site <o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | </tbody></table>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | | + | |
− | <div class="row" style="height:5vw;"></div>
| + | |
− | | + | |
− | <div class = "row">
| + | |
− | <div class = "content" id="please">
| + | |
− | <center><h1>SILVER</h1></center>
| + | |
− | <div class="box2"></div>
| + | |
− | <div class="row" style="height:2vw;"></div>
| + | |
− | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="99%" style="width:99.54%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| + | |
− | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| + | |
− | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.3pt">
| + | |
− | <td colspan="4" style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
| + | |
− | background:#828282;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:20.0pt;font-family:Quicksand;color:white;mso-themecolor:
| + | |
− | background1">SILVER<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:1;height:18.3pt">
| + | |
− | <td width="149" style="width:148.6pt;border:solid windowtext 1.0pt;border-top:
| + | |
− | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| + | |
− | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/ <span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="106" style="width:106.3pt;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| + | |
− | height:18.3pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TYPE<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="108" style="width:108.3pt;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| + | |
− | height:18.3pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">SOURCE<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="334" style="width:334.3pt;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| + | |
− | height:18.3pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">KEY FEATURES<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:2;height:11.35pt">
| + | |
− | <td width="149" style="width:148.6pt;border:solid windowtext 1.0pt;border-top:
| + | |
− | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| + | |
− | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span> <o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">BBa_K2417003<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="106" style="width:106.3pt;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="108" style="width:108.3pt;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| + | |
− | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| + | |
− | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="334" style="width:334.3pt;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| + | |
− | <p class="MsoListParagraphCxSpFirst"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></b></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain
| + | |
− | insulin analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker
| + | |
− | (QRGGGSGGGQRR)<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His
| + | |
− | Tag<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible
| + | |
− | linker<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease
| + | |
− | cleavage site<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome
| + | |
− | binding site<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | <p class="MsoListParagraphCxSpLast"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></b></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:3;height:114.0pt">
| + | |
− | <td width="149" rowspan="2" style="width:148.6pt;border:solid windowtext 1.0pt;
| + | |
− | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| + | |
− | padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">BBa_k2417000<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="106" rowspan="2" style="width:106.3pt;border-top:none;border-left:
| + | |
− | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="108" style="width:108.3pt;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand">E. Coli </span></i><span lang="EN-US" style="font-size:
| + | |
− | 14.0pt;font-family:Quicksand">(<span class="SpellE">Ecotin</span> Tag)<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="334" rowspan="2" style="width:334.3pt;border-top:none;border-left:
| + | |
− | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt">
| + | |
− | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpFirst" style="text-indent:-18.0pt;mso-list:l4 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span> tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l4 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N <span class="GramE">terminal <span style="mso-spacerun:yes"> </span>x</span> His
| + | |
− | Tag <o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l4 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible
| + | |
− | linker<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l4 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease
| + | |
− | cleavage site <b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l4 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosomal
| + | |
− | binding site<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | <p class="MsoListParagraphCxSpLast"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></b></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:114.0pt">
| + | |
− | <td width="108" style="width:108.3pt;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| + | |
− | proinsulin)<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | </tbody></table>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | | + | |
− | <div class="row" style="height:5vw;"></div>
| + | |
− | | + | |
− | <div class = "row">
| + | |
− | <div class = "content" id="please">
| + | |
− | <center><h1>BRONZE</h1></center>
| + | |
− | <div class="box3"></div>
| + | |
− | <div class="row" style="height:2vw;"></div>
| + | |
− | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| + | |
− | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| + | |
− | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
| + | |
− | <td width="100%" colspan="4" style="width:100.0%;border:solid windowtext 1.0pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;background:#A36952;padding:0cm 5.4pt 0cm 5.4pt;
| + | |
− | height:4.75pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand"><o:p> </o:p></span></b></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:20.0pt;font-family:Quicksand;color:white;mso-themecolor:
| + | |
− | background1">BRONZE<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand"><o:p> </o:p></span></b></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:1;height:4.75pt">
| + | |
− | <td width="21%" style="width:21.3%;border:solid windowtext 1.0pt;border-top:
| + | |
− | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| + | |
− | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand"><o:p> </o:p></span></b></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></b></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand"><o:p> </o:p></span></b></p>
| + | |
− | </td>
| + | |
− | <td width="15%" style="width:15.24%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
| + | |
− | height:4.75pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand"><o:p> </o:p></span></b></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand">TYPE<o:p></o:p></span></b></p>
| + | |
− | </td>
| + | |
− | <td width="15%" style="width:15.5%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
| + | |
− | height:4.75pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand"><o:p> </o:p></span></b></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand">SOURCE<o:p></o:p></span></b></p>
| + | |
− | </td>
| + | |
− | <td width="47%" style="width:47.96%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
| + | |
− | height:4.75pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand"><o:p> </o:p></span></b></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand">KEY FEATURES<o:p></o:p></span></b></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:2;height:35.15pt">
| + | |
− | <td width="21%" rowspan="2" style="width:21.3%;border:solid windowtext 1.0pt;
| + | |
− | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| + | |
− | padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">BBa_K2417002<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="15%" rowspan="2" style="width:15.24%;border-top:none;border-left:
| + | |
− | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
| + | |
− | </td>
| + | |
− | <td width="15%" style="width:15.5%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand"><o:p> </o:p></span></i></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand">E. Coli </span></i><span lang="EN-US" style="font-size:
| + | |
− | 14.0pt;font-family:Quicksand">(<span class="SpellE">Ecotin</span> Tag)<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand"><o:p> </o:p></span></b></p>
| + | |
− | </td>
| + | |
− | <td width="47%" rowspan="2" style="width:47.96%;border-top:none;border-left:
| + | |
− | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| + | |
− | <p class="MsoListParagraphCxSpFirst" style="text-indent:-18.0pt;mso-list:l2 level1 lfo6"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain
| + | |
− | insulin analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker
| + | |
− | (QRGGGSGGGQRR)<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span> tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His
| + | |
− | Tag<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible
| + | |
− | linker <b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease
| + | |
− | cleavage site<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | <p class="MsoListParagraphCxSpLast" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
− | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome
| + | |
− | binding site<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | <tr style="mso-yfti-irow:3;mso-yfti-lastrow:yes;height:18.0pt">
| + | |
− | <td width="15%" style="width:15.5%;border-top:none;border-left:none;
| + | |
− | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| + | |
− | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| + | |
− | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand"><o:p> </o:p></span></i></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;
| + | |
− | font-family:Quicksand">N/A </span></i><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:
| + | |
− | Helvetica;mso-bidi-font-family:Helvetica">–</span></i><i style="mso-bidi-font-style:
| + | |
− | normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"> </span></i><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| + | |
− | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | </tbody></table>
| + | |
− | </div>
| + | |
| </div> | | </div> |
| | | |
− | <div class="row" style="height:10vw;"> | + | <div class ="row" style="height:5vw;"></div> |
| + | <div id="please"><h6> Below is a table showing all the parts we have submitted to the registry. Please see our <a href="https://2017.igem.org/Team:Sydney_Australia/Basic_Part">basic parts </a> and <a href="https://2017.igem.org/Team:Sydney_Australia/Composite_Part">composite parts</a> pages for more information on each part, as well as our <a href="https://2017.igem.org/Team:Sydney_Australia/Improve">part improvements page</a> to see how we improved upon previously registered parts.</h6></div> |
| + | <center> |
| + | </html><groupparts>iGEM17 Sydney_Australia</groupparts> |
| + | <html> |
| | | |
| + | <div class="row" style="height:10vw;"></div> |
| </div> | | </div> |
| </body> | | </body> |
| </html> | | </html> |
| {{:Team:Sydney_Australia/templates/Footer}} | | {{:Team:Sydney_Australia/templates/Footer}} |