Difference between revisions of "Team:Sydney Australia/Parts"

 
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</head>
 
</head>
 
<body>
 
<body>
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<div class="container-fluid">
  
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<div class = "row" style ="background-color:#e2e2e2;margin-top:60px;">
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<div class = "bannerbox" style ="background-color:#e2e2e2">
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    <img class="center-block img-center" src="https://static.igem.org/mediawiki/2017/4/48/T--Sydney_Australia--partspage_banner.png" width="80%">
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<div class = "center">
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    <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Sydney_Australia--PartsBanner.gif" width="70%">
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</div>
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</div>
 
</div>
  
 
<div class ="row" style="height:5vw;"></div>
 
<div class ="row" style="height:5vw;"></div>
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<div id="please"><h6> Below is a table showing all the parts we have submitted to the registry. Please see our <a href="https://2017.igem.org/Team:Sydney_Australia/Basic_Part">basic parts </a> and <a href="https://2017.igem.org/Team:Sydney_Australia/Composite_Part">composite parts</a> pages for more information on each part, as well as our <a href="https://2017.igem.org/Team:Sydney_Australia/Improve">part improvements page</a> to see how we improved upon previously registered parts.</h6></div>
 +
<center>
 +
</html><groupparts>iGEM17 Sydney_Australia</groupparts>
 +
<html>
  
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<div class = "row">
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<div class="row" style="height:10vw;"></div>
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<div class = "content" id="please">
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<center><h1>GOLD</h1></center>
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<div class="box1"></div>
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<div class="row" style="height:2vw;"></div>
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<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
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  solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>SOURCE<o:p></o:p></span></p>
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  </td>
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  <td width="29%" style="width:29.62%;border:solid windowtext 1.0pt;border-left:
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>KEY FEATURES<o:p></o:p></span></p>
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  </td>
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  <td width="41%" style="width:41.56%;border:solid windowtext 1.0pt;border-left:
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>WHAT IS IT GOLD?<o:p></o:p></span></p>
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  </td>
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</tr>
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<tr style="mso-yfti-irow:1;height:78.9pt">
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  <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
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  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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−
  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
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−
  </td>
+
−
  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  </td>
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−
  <td width="29%" style="width:29.62%;border-top:none;border-left:none;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
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  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
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  (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
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−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
+
−
  translocate protein into media<o:p></o:p></span></p>
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−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
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  purification<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
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  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
+
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  demonstrated that it works:<o:p></o:p></span></p>
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  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
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  insulin-sensitive cell lines and saw an increase in glycogen synthesis and
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  glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
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  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
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  <!--[endif]--><o:p></o:p></span></p>
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  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
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  ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
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  the media in which the cells were growing did. <span class="GramE">Therefore</span>
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  the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
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  <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
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  font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
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  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
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  </td>
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</tr>
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<tr style="mso-yfti-irow:2;height:206.35pt">
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  <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
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  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
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  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
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  tag)<o:p></o:p></span></p>
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  </td>
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  <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
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  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
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  tag for targeting proteins to the periplasm<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">trypsin protease cleavage site
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  for His-tag and C-peptide cleavage<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
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  site<o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
+
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  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
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  <!--[endif]--><o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
+
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  improved previous parts:<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their sequence<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
+
−
  transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
+
−
  site<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
+
−
  for purification<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
+
−
  demonstrated that it works:<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
+
−
  insulin-sensitive cell lines and saw an increase in glycogen synthesis and
+
−
  glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
+
−
  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:3;height:72.85pt">
+
−
  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
+
−
  proinsulin)<o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
+
−
  <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
+
−
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes">&nbsp;&nbsp;&nbsp;&nbsp;</span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
+
−
  proinsulin)<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="29%" style="width:29.62%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
+
−
  for His-tag and C-peptide cleavage<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
+
−
  site<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
+
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
+
−
  improved previous parts:<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their
+
−
  sequence<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
+
−
  ribosomal binding site<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
+
−
  N-terminal His tag for purification<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
+
−
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
+
−
  font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
+
−
  cell lines and saw an increase in glycogen synthesis and glucose oxidation
+
−
  above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
+
−
  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
</tbody></table>
+
−
</div>
+
−
</div>
+
−
 
+
−
<div class="row" style="height:5vw;"></div>
+
−
 
+
−
<div class = "row">
+
−
<div class = "content" id="please">
+
−
<center><h1>SILVER</h1></center>
+
−
<div class="box2"></div>
+
−
<div class="row" style="height:2vw;"></div>
+
−
<div align="center">
+
−
<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+
−
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
+
−
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
+
−
  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
+
−
  solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
+
−
  height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/<span style="mso-spacerun:yes">&nbsp; </span>NO.</span><o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
+
−
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br>SOURCE<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
+
−
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br>KEY FEATURES<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
+
−
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">WHY IS IT SILVER?<o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
+
−
  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
+
−
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.56%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
+
−
  mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
+
−
  14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="30%" style="width:30.12%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
+
−
  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
+
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
+
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  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
+
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  parts by showing that they work in producing insulin. We performed an ELISA
+
−
  assay testing for properly folded insulin, and detected the presence of
+
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  Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
+
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  results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
+
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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</tr>
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</tbody></table>
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</div>
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</div>
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</div>
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<div class="row" style="height:5vw;"></div>
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<div class = "row">
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<div class = "content" id="please">
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<center><h1>BRONZE</h1></center>
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<div class="box3"></div>
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<div class="row" style="height:2vw;"></div>
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<div align="center">
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<div align="center">
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<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
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  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
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  solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
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  height:4.75pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br>SOURCE<o:p></o:p></span></p>
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  </td>
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  <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br>KEY FEATURES<o:p></o:p></span></p>
+
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  </td>
+
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  <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">WHY IS IT BRONZE?<o:p></o:p></span></p>
+
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  </td>
+
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</tr>
+
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<tr style="mso-yfti-irow:1;height:35.15pt">
+
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  <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
+
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  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
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  padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.48%;border-top:none;border-left:none;
+
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  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
+
−
  Tag)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
+
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  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
+
−
  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
+
−
  tag for targeting proteins to the periplasm<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
+
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  </td>
+
−
  <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
+
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  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
+
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  the registry, for other teams to use in the future<o:p></o:p></span></p>
+
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  </td>
+
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+
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<tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
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  <td width="12%" style="width:12.48%;border-top:none;border-left:none;
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  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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+
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
+
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  mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
+
−
  14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  </td>
+
−
</tr>
+
−
</tbody></table>
+
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+
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+
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Latest revision as of 13:53, 1 November 2017

Below is a table showing all the parts we have submitted to the registry. Please see our basic parts and composite parts pages for more information on each part, as well as our part improvements page to see how we improved upon previously registered parts.
<groupparts>iGEM17 Sydney_Australia</groupparts>