Difference between revisions of "Team:Sydney Australia/Parts"

 
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    <img class="center-block img-center" src="https://static.igem.org/mediawiki/2017/4/48/T--Sydney_Australia--partspage_banner.png" width="80%">
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    <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Sydney_Australia--PartsBanner.gif" width="70%">
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<div id="please"><h6> Below is a table showing all the parts we have submitted to the registry. Please see our <a href="https://2017.igem.org/Team:Sydney_Australia/Basic_Part">basic parts </a> and <a href="https://2017.igem.org/Team:Sydney_Australia/Composite_Part">composite parts</a> pages for more information on each part, as well as our <a href="https://2017.igem.org/Team:Sydney_Australia/Improve">part improvements page</a> to see how we improved upon previously registered parts.</h6></div>
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<center>
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<div class = "content" id="please" width="80%">
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</html><groupparts>iGEM17 Sydney_Australia</groupparts>
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<center><h1>COMPOSITE PARTS</h1></center>
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<div class="box5"></div>
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<h4>All of our gold, silver and bronze winning parts are composite parts, consisting of a multitude of basic parts. The complete sequences and characteristics of these basic parts are linked on the Parts Registry pages of our composite parts (linked in the table).</h4>
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<div class = "row">
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<div class = "content" id="please">
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<center><h1>GOLD</h1></center>
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<div class="box1"></div>
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<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME /<br> NO.<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>SOURCE<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>KEY FEATURES<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>WHY IS IT GOLD?<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
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  </td>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br>Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <td width="29%" style="width:29.62%;border-top:none;border-left:none;
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
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  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
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  (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
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  translocate protein into media<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
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  purification<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
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  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
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  demonstrated that it works:<o:p></o:p></span></p>
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  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
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  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
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  insulin-sensitive cell lines and saw an increase in glycogen synthesis and
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  glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
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  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
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  <!--[endif]--><o:p></o:p></span></p>
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  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
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  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
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  ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
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  the media in which the cells were growing did. <span class="GramE">Therefore</span>
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  the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
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  <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
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  font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
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  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
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  </td>
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</tr>
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<tr style="mso-yfti-irow:2;height:206.35pt">
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  <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
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  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br>E. Coli (<span class="SpellE">Ecotin</span>
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  tag)<o:p></o:p></span></p>
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  <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
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  tag for targeting proteins to the periplasm<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">trypsin protease cleavage site
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  for His-tag and C-peptide cleavage<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
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  site<o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
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  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
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  <!--[endif]--><o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
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  improved previous parts:<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their sequence<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
+
−
  transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
+
−
  site<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
+
−
  for purification<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
+
−
  demonstrated that it works:<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
+
−
  insulin-sensitive cell lines and saw an increase in glycogen synthesis and
+
−
  glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
+
−
  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:3;height:72.85pt">
+
−
  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br>Human (<span class="SpellE">Human</span>
+
−
  proinsulin)<o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
+
−
  <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
+
−
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
    <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
      <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
        <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
          <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br<br><br>Cytoplasmic-Proinsulin <span style="mso-spacerun:yes">&nbsp;&nbsp;&nbsp;&nbsp;</span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Human (<span class="SpellE">Human</span>
+
−
  proinsulin)<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="29%" style="width:29.62%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
+
−
  for His-tag and C-peptide cleavage<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
+
−
  site<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
+
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
+
−
  improved previous parts:<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their
+
−
  sequence<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
+
−
  ribosomal binding site<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
+
−
  N-terminal His tag for purification<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
+
−
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
+
−
  font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
+
−
  cell lines and saw an increase in glycogen synthesis and glucose oxidation
+
−
  above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
+
−
  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
</tbody></table>
+
−
</div>
+
−
</div>
+
−
 
+
−
<div class="row" style="height:5vw;"></div>
+
−
 
+
−
<div class = "row">
+
−
<div class = "content" id="please">
+
−
<center><h1>SILVER</h1></center>
+
−
<div class="box2"></div>
+
−
<div class="row" style="height:2vw;"></div>
+
−
<div align="center">
+
−
<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+
−
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
+
−
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
+
−
  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
+
−
  solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
+
−
  height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME /<br> NO.</span><o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
+
−
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
+
−
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
+
−
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
+
−
  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
+
−
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
    <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
      <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
      <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.56%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
+
−
  mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
+
−
  14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="30%" style="width:30.12%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
+
−
  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
+
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
+
−
  parts by showing that they work in producing insulin. We performed an ELISA
+
−
  assay testing for properly folded insulin, and detected the presence of
+
−
  Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
+
−
  results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
</tbody></table>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
 
+
−
<div class="row" style="height:5vw;"></div>
+
−
 
+
−
<div class = "row">
+
−
<div class = "content" id="please">
+
−
<center><h1>BRONZE</h1></center>
+
−
<div class="box3"></div>
+
−
<div class="row" style="height:2vw;"></div>
+
−
<div align="center">
+
−
<div align="center">
+
−
 
+
−
<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+
−
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
+
−
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
+
−
  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
+
−
  solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
+
−
  height:4.75pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME /<br> NO.<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
+
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
+
−
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
+
−
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:1;height:35.15pt">
+
−
  <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
+
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.48%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>E. Coli (<span class="SpellE">Ecotin</span>
+
−
  Tag)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
+
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
+
−
  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
+
−
  tag for targeting proteins to the periplasm<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
+
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
+
−
  the registry, for other teams to use in the future<o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
+
−
  <td width="12%" style="width:12.48%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
+
−
  mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
+
−
  14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
</tbody></table>
+
−
 
+
−
</div>
+
−
</div>
+
−
</div>
+
−
</div>
+
  
 
<div class="row" style="height:10vw;"></div>
 
<div class="row" style="height:10vw;"></div>
−
 
−
<div class = "row">
 
−
<div class = "content" id="please">
 
−
<center><h1>BASIC PARTS</h1></center>
 
−
<div class="box4"></div>
 
−
<div class="row" style="height:2vw;"></div>
 
−
<div style="text-align:center">
 
−
<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt; mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
 
−
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.4pt">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  mso-border-alt:solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:
 
−
  accent1;mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 
−
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand"><br>
 
−
  PART NO.<o:p></o:p></span></b></p>
 
−
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border:solid windowtext 1.0pt;
 
−
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 
−
  solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:accent1;
 
−
  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 
−
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand"><br>
 
−
  PART NAME<o:p></o:p></span></b></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border:solid windowtext 1.0pt;
 
−
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 
−
  solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:accent1;
 
−
  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 
−
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand"><br>
 
−
  SOURCE<o:p></o:p></span></b></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border:solid windowtext 1.0pt;
 
−
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 
−
  solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:accent1;
 
−
  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 
−
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand"><br>
 
−
  DESCRIPTION<o:p></o:p></span></b></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:1">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417004">BBa_K2417004</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Glycine Glycine Serine (x4) Linker Motif A<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Designed<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Linker motif used
 
−
  to provide spacer region<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Used to expose
 
−
  affinity purification tags such as 6x His tag<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Serine and glycine
 
−
  used as they are small and flexible<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:2">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417006">BBa_K2417006</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human Proinsulin Coding Sequence<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Humans<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Coding sequence for
 
−
  human proinsulin, sourced from NCIB<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Consists of A, B
 
−
  and C chains, but C-peptide is cleaved out<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Contains 3 <span class="SpellE">disulphide</span> bonds<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Contains arginine
 
−
  residues adjacent to C-peptide to allow cleavage<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:3">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417007">BBa_K2417007</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand">Winsulin - Single Chain Insulin Analogue<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Designed<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Consists of short linker peptide instead of C-peptide<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Does not require cleavage therefore simplifies production procedures<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Linker peptide raises <span class="SpellE">pI</span> of insulin analogue (long lasting after administration)<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Increased <span class="SpellE">thermostability</span> (based on other single chain insulin analogues tested)<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:4">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417008">BBa_K2417008</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"> 6x Poly-Histidine Tag codon optimized for use in <i style="mso-bidi-font-style:
 
−
  normal">E. coli</i><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br> Designed (optimized for use in <i style="mso-bidi-font-style:normal">Escherichia coli</i>)<br style="mso-special-character:line-break">
 
−
  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
 
−
  <!--[endif]--><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l6 level1 lfo6"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Codon optimized for use in <i style="mso-bidi-font-style:normal">E. coli</i><o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l6 level1 lfo6"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Used to immobilize ions on a chromatography affinity column (nickel, cobalt or copper)<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l6 level1 lfo6"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Small, and easy to add to the ends of proteins of interest<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:5;height:27.45pt">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
 
−
  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
 
−
  <!--[endif]--><o:p></o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417009">BBa_K2417009</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br>Extended ribosome binding site<o:p></o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">T7 major capsid protein<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l3 level1 lfo7"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">High ribosomal recruitment efficiency<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l3 level1 lfo7"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Sourced from the pET15b vectors<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:6">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417010">BBa_K2417010</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-size:
 
−
  14.0pt;font-family:Quicksand"> Periplasmic Signal Peptide<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Escherichia coli<o:p></o:p></span></i></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Small periplasmic, <span class="SpellE">homodimeric</span> protein<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Upon fusion to N-terminus of protein will translocate protein to periplasm of <i style="mso-bidi-font-style:normal">E. coli</i><o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Periplasm is an oxidative environment ideal for protein folding, and simplifies extraction due to eliminating the need for purifying inclusion bodies from the cytoplasm<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Periplasm has reduced proteases<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:7">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
 
−
  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
 
−
  <!--[endif]--><o:p></o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417011">BBa_K2417011</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand">YncM Bacillus Secretion Peptide<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Bacillus subtilis<o:p></o:p></span></i></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l0 level1 lfo9"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Can be fused to the
 
−
  N-terminus of proteins to induce secretion to the surrounding media wheN proteins are expressed in <i style="mso-bidi-font-style:normal">Bacillus subtilis</i><o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l0 level1 lfo9"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Eliminates many
 
−
  steps in the purification process<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:8;height:85.45pt">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417012">BBa_K2417012<o:p></o:p></a></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV Protease Cleavage Sequence<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A<o:p></o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l4 level1 lfo10"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Sequence for which TEV protease is specific to<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l4 level1 lfo10"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Can be added between protein of interest and peptides that require removal, such as purification tags<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:9;mso-yfti-lastrow:yes">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417013">BBa_K2417013</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Glycine
 
−
  <span class="SpellE">Glycine</span> Serine (x4) Linker <o:p></o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Motif
 
−
  B<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Designed<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Linker motif used
 
−
  to provide spacer region<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Used to expose
 
−
  affinity purification tags such as 6x His tag<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Serine and glycine
 
−
  used as they are small and flexible<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
</tbody>
 
−
</table>
 
 
</div>
 
</div>
−
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−
</div>
 
−
 
−
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−
 
 
</body>
 
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Latest revision as of 13:53, 1 November 2017

Below is a table showing all the parts we have submitted to the registry. Please see our basic parts and composite parts pages for more information on each part, as well as our part improvements page to see how we improved upon previously registered parts.
<groupparts>iGEM17 Sydney_Australia</groupparts>