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− | <p><i>Background</i><br><br> | + | <p><i><strong>Background</strong></i><br><br> |
In <a href="https://2017.igem.org/Team:ITB_Indonesia" target="_blank">Team ITB_Indonesia 2017</a> | In <a href="https://2017.igem.org/Team:ITB_Indonesia" target="_blank">Team ITB_Indonesia 2017</a> | ||
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increasing the expression of mRFP.<br><br> | increasing the expression of mRFP.<br><br> | ||
− | <i>Experimental Design</i><br><br> | + | <i><strong>Experimental Design</strong></i><br><br> |
We used three different strains of transformed <i>E. coli</i> (BL21, DH5alpha, and Top10) for this | We used three different strains of transformed <i>E. coli</i> (BL21, DH5alpha, and Top10) for this | ||
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<tr> | <tr> | ||
<td> | <td> | ||
− | <p><i>Result and Findings</i><br> | + | <p><i><strong>Result and Findings</strong></i><br> |
<ul> | <ul> | ||
<li>There are no significant differences of mRFP expression in different strains of <i>E. coli</i> | <li>There are no significant differences of mRFP expression in different strains of <i>E. coli</i> | ||
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<li>The broth become red in color under natural light around 16-20 hours of incubation time.</li> | <li>The broth become red in color under natural light around 16-20 hours of incubation time.</li> | ||
</ul> | </ul> | ||
− | <br></p> | + | <br> |
+ | <figure><img src=" | ||
+ | https://static.igem.org/mediawiki/2017/0/06/Foto_mRFP1.jpg" style="height: 45%; width: 45%;"/> | ||
+ | <figcaption><i><b>Figure 2:</b> ~18 hours old mRFP-transformed E. coli strains (Right: control)</i></figcaption></figure> | ||
+ | |||
+ | <figure><img src=" | ||
+ | https://static.igem.org/mediawiki/2017/2/2c/Foto_mRFP2.jpg" style="height: 45%; width: 45%;"/> | ||
+ | <figcaption><i><b>Figure 3:</b> ~30 hours old mRFP-transformed E. coli strains (Left: control). Notice the color turn to bright purple.</i></figcaption></figure> | ||
+ | |||
+ | </p> | ||
</td> | </td> | ||
<td> | <td> | ||
− | <img src=" | + | <figure><img src=" |
− | https://static.igem.org/mediawiki/ | + | https://static.igem.org/mediawiki/parts/7/72/Top10woIPTG.JPG" style="height: 75%; width: 75%;"/> |
+ | <figcaption><i><b>Figure 4:</b> Red color dynamics of transformed E. coli Top10 compared to control</i></figcaption></figure> | ||
+ | |||
+ | <figure><img src=" | ||
+ | https://static.igem.org/mediawiki/parts/b/bd/DH5woIPTG.jpeg" style="height: 75%; width: 75%;"/> | ||
+ | <figcaption><i><b>Figure 5:</b> Red color dynamics of transformed E. coli DH5alpha compared to control</i></figcaption></figure> | ||
+ | |||
+ | <figure><img src=" | ||
+ | https://static.igem.org/mediawiki/parts/b/b7/BL21wolIPTG.jpeg" style="height: 75%; width: 75%;"/> | ||
+ | <figcaption><i><b>Figure 6:</b> Red color dynamics of transformed E. coli BL21 compared to control</i></figcaption></figure> | ||
</td> | </td> | ||
</tr> | </tr> |
Latest revision as of 16:50, 1 November 2017
Contribution
New characterization to RFP Coding Device
Background |
Result and Findings
|