Difference between revisions of "Team:REC-CHENNAI/Safety"

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<div class="site_title">SAFETY</div>
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<h1> Safety </h1>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h1>INTRODUCTION</h1>
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People who work in bioscience facilities and research environments can be exposed to a variety of chemical, biological or physical hazards as they perform daily tasks. It is therefore critical that the lab safety rules and regulations are strictly adhered to.
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                      We have a Bio Safety Committee in our institution. It consists of various members from our department who ensure that safety guidelines as per Department of Biotechnology (DBT), India norms are followed meticulously. Our team members received a safety training from professors and lab assistants from this committee to manage risks from accidents that may occur while working in the lab. During this training, we were taught about a range of topics right from sterilization techniques to emergency procedures that need to be followed. It was insisted that we were gloves, especially while handling carcinogens; that all the plates be decanted before discarding them. We carried out all lab procedures as taught by our department specialists. 
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<h1>PROJECT SAFETY</h1>
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Our lab is a level 2 (moderate risk) safety level environment. That is, we have open benches and bio-safety cabinets.
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For our project, we are making E.coli Dh5 alpha produce an antimicrobial peptide.
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Cloning was carried out as per the DBT rules and regulations.
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We will be testing its antimicrobial activity against <i>Bacillus subtilis</i> and <i>Saccharomyces cerevisiae</i>. All these organisms fall under the Risk group 1 organisms as listed in the iGEM website. We worked with them in separate bio safety cabinets only.
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<h5>Safe Project Design</h5>
 
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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<h5>Safe Lab Work</h5>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h5>Safe Shipment</h5>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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Latest revision as of 17:31, 1 November 2017