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We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in <i>S. cerevisiae</i>.</li>
 
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  <li>We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in <i>S. cerevisiae</i>.</li>
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By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents
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With two different sensors we were able to efficiently  measure the pH and the redox potential inside our yeast peroxisomes.
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Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible.
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By successfully translocating the required enzymes for the metabolic pathways of nootkatone and violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox
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We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox.
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With a high throughput assay we characterized the import efficiency of different PTS2 sequences.
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To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment.
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For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time.
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All our excellent results can be combined into a highly variable compartment toolbox for designing artificial compartments based on the peroxisomes in <i>S. cerevisiae</i> with an enormous range of applications.
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Revision as of 19:29, 1 November 2017

  • We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in S. cerevisiae.