Difference between revisions of "Team:ITB Indonesia/Part Collection"

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<h1 class="ITB_h1">Part Collection</h1>
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<h1 class="ITB_h1">Parts</h1>
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<p style="font-style: italic;"><strong>Parts Collection</strong></p>
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<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color: #1c2922">Basic Parts</h1>
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<h2>This is the parts collection designed by ITB Indonesia iGEM Team 2017.</h2>
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<p>:</p>
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  <tr>
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    <th>Part Name</th>
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    <th>BioBrick</th>
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    <th>Description</th>
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    <th>Part Type</th>
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  </tr>
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    <td>PETase</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2378001">BBa_K2378001</a></td>
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    <td>This is optimized coding sequence of PETase enzyme for <i>E. coli</i> BL21.
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<td>Coding</td
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  <tr>
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    <td>
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IrrE Coding Sequence
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</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2378007">BBa_K2378007</a></td>
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    <td>This is improved coding sequence of IrrE from BBa_K729001 (http://parts.igem.org/Part:BBa_K729001). When transformed to E. coli, this protein protected from salt, oxidative, and thermal shock.
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<td>Coding</td
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  <tr>
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    <td>EndA Endonuclease Coding Sequence from Vibrio cholera
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</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2378008">BBa_K2378008</a></td>
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    <td>This endonuclease degrades free extracellular DNA. The gene is isolated from Vibrio cholera.
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</td>
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<td>Coding</td
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<tr>
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    <td>pSal + PETase Coding Device
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</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2378004">BBa_K2378004</a></td>
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    <td>ITB_Indonesia iGEM team 2017 designed a PETase enzyme coding device consisting of NahR-pSal regulatory part (BBa_J61051, http://parts.igem.org/Part:BBa_J61051) which is activated by salicylate inducer. In effect, PETase is only expressed when this device detect the presence of salicylate, which binds to NahR.
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</td>
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<td>Composite</td
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    <td>
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Constitutive PETase Coding Device
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</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2378005">BBa_K2378005</a></td>
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    <td>This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of constitutive promoter BBa_J23106.
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</td>
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<td>Composite</td
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  </tr>
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  <tr>
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    <td>NhaR-PETase Constitutive Coding Device
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</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2378006">BBa_K2378006</a></td>
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    <td>This is an optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of NhaR BBa_K1357002 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K1357002) coding sequence. NhaR enhances the transcription of pgaABCD operon which in turn induces the production of biofilm. This part also uses constitutive promoters BBa_J23106.
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</td>
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<td>Composite</td
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  </tr>
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    <tr>
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    <td>EndA Endonuclease Coding Device from Vibrio cholera
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</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2378009">BBa_K2378009</a></td>
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    <td>This is coding device of endonuclease degrades free extracellular DNA, with constitutive promoter BBa_J61051. The gene is isolated from Vibrio cholera.
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</td>
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<td>Composite</td
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Revision as of 19:41, 1 November 2017

Team Attributions
Collaborations Overview Design Notebook Contribution Results Improve
Basic Parts Composite Part Part Collection
Silver HP Integrated and Gold Public Engagement


Parts

Parts Collection


Basic Parts

This is the parts collection designed by ITB Indonesia iGEM Team 2017.

:

Part Name BioBrick Description Part Type
PETase BBa_K2378001 This is optimized coding sequence of PETase enzyme for E. coli BL21. Coding
IrrE Coding Sequence BBa_K2378007 This is improved coding sequence of IrrE from BBa_K729001 (http://parts.igem.org/Part:BBa_K729001). When transformed to E. coli, this protein protected from salt, oxidative, and thermal shock. Coding
EndA Endonuclease Coding Sequence from Vibrio cholera BBa_K2378008 This endonuclease degrades free extracellular DNA. The gene is isolated from Vibrio cholera. Coding
pSal + PETase Coding Device BBa_K2378004 ITB_Indonesia iGEM team 2017 designed a PETase enzyme coding device consisting of NahR-pSal regulatory part (BBa_J61051, http://parts.igem.org/Part:BBa_J61051) which is activated by salicylate inducer. In effect, PETase is only expressed when this device detect the presence of salicylate, which binds to NahR. Composite
Constitutive PETase Coding Device BBa_K2378005 This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of constitutive promoter BBa_J23106. Composite
NhaR-PETase Constitutive Coding Device BBa_K2378006 This is an optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of NhaR BBa_K1357002 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K1357002) coding sequence. NhaR enhances the transcription of pgaABCD operon which in turn induces the production of biofilm. This part also uses constitutive promoters BBa_J23106. Composite
EndA Endonuclease Coding Device from Vibrio cholera BBa_K2378009 This is coding device of endonuclease degrades free extracellular DNA, with constitutive promoter BBa_J61051. The gene is isolated from Vibrio cholera. Composite