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| | <tr> | | <tr> |
| | <td>We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in <i>S. cerevisiae<i></td> | | <td>We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in <i>S. cerevisiae<i></td> |
| − | <td>WTF</td> | + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| | </tr> | | </tr> |
| | | | |
| | <tr> | | <tr> |
| − | <td></td> | + | <td>By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents</td> |
| − | <td>WTF</td> | + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| | </tr> | | </tr> |
| | | | |
| | <tr> | | <tr> |
| − | <td></td> | + | <td>With two different sensors we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes</td> |
| − | <td>WTF</td> | + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| | </tr> | | </tr> |
| | | | |
| − | </tbody>
| + | <tr> |
| − | </table> | + | <td>Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible</td> |
| | + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| | + | </tr> |
| | | | |
| | + | <tr> |
| | + | <td>By successfully translocating the required enzymes for the metabolic pathways of Nootkatone and Violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox</td> |
| | + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| | + | </tr> |
| | | | |
| | + | <tr> |
| | + | <td>We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox</td> |
| | + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| | + | </tr> |
| | | | |
| | + | <tr> |
| | + | <td>With a high throughput assay we characterized the import efficiency of different PTS2 sequences</td> |
| | + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| | + | </tr> |
| | | | |
| − | <table> | + | <tr> |
| − | <tbody>
| + | <td>To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment</td> |
| − | <tr>
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| − | <td><div><a href="https://2017.igem.org/Team:Aix-Marseille ">MAX MUSTERMANN</a> align="right"</div></td>
| + | </tr> |
| − | <td>SCHEIßE</td>
| + | |
| − | <td>SCHEIßE2</td>
| + | |
| − | </tr>
| + | |
| | | | |
| − | <tr>
| + | <tr> |
| − | <td><a href="https://2017.igem.org/Team:Aachen">Aachen</a></td>
| + | <td>For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time |
| − | <td><a href="https://2017.igem.org/Team:Aalto-Helsinki/Collaborations">Aalto-Helsinki</a></td>
| + | </td> |
| − | <td><a href="https://2017.igem.org/Team:Aix-Marseille ">Aix-Marseille</a></td>
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| − | </tr>
| + | </tr> |
| − | <tr>
| + | |
| − | <td><a href="https://2017.igem.org/Team:Bielefeld-CeBiTec ">Bielefeld-CeBiTec</a></td>
| + | |
| − | <td><a href="https://2017.igem.org/Team:UBonn_HBRS ">UBonn_HBRS</a></td>
| + | |
| − | <td><a href="https://2017.igem.org/Team:ColumbiaNYC">ColumbiaNYC</a></td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| | | | |
| | + | <tr> |
| | + | <td>All our excellent results can be combined into a highly variable compartment toolbox for designing artificial compartments based on the peroxisomes in <i>S. cerevisiae</i> with an enormous range of applications |
| | + | </td> |
| | + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| | + | </tr> |
| | | | |
| | | | |
| − | <img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">
| + | </tbody> |
| − | | + | |
| − | | + | |
| − | <table>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td>HIER DEN TEXT</td>
| + | |
| − | <td><div align="right">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;">
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | | + | |
| − | </tbody> | + | |
| | </table> | | </table> |
| | | | |
| | | | |
| − |
| |
| − |
| |
| − |
| |
| − | <div class="callout">
| |
| − | <h2>Results and engineered constructs of artico</h2>
| |
| − | <ul>
| |
| − | <img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg">We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in <i>S. cerevisiae<i>
| |
| − | </li>
| |
| − | <li>By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents
| |
| − | </li>
| |
| − | <li>With two different sensors we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes.
| |
| − | </li>
| |
| − | <li>Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible.
| |
| − | </li>
| |
| − | <li>By successfully translocating the required enzymes for the metabolic pathways of Nootkatone and Violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox
| |
| − | </li>
| |
| − | <li>We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox.
| |
| − | </li>
| |
| − | <li>With a high throughput assay we characterized the import efficiency of different PTS2 sequences.
| |
| − | </li>
| |
| − | <li>To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment.
| |
| − | </li>
| |
| − | <li>For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time.
| |
| − | </li>
| |
| − | <li>All our excellent results can be combined into a highly variable compartment toolbox for designing artificial compartments based on the peroxisomes in <i>S. cerevisiae</i> with an enormous range of applications.
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| | | | |
| | | | |
