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| <h1>Testheading</h1> | | <h1>Testheading</h1> |
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− | | + | <div class="callout"> |
| <table> | | <table> |
| <tbody> | | <tbody> |
| <tr> | | <tr> |
− | <td>OMG</td> | + | <th><h3 style="color:rgb(0,0,0)"><a style="color:rgb(0,0,0)" href="https://2017.igem.org/Team:Cologne-Duesseldorf/Results">Results</h3></th> |
− | <td>WTF</td> | + | <th><h3 style="color:rgb(0,0,0)">Achieved</h3></th> |
| </tr> | | </tr> |
− | </tbody>
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− | </table>
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| + | <tr> |
| + | <td>We were able to design and successfully test an orthogonal peroxisomal protein <a href="https://2017.igem.org/Team:Cologne-Duesseldorf/Results#PEX5Import">import mechanism</a> for peroxisomes in <i>S. cerevisiae<i></td> |
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| + | </tr> |
| | | |
| + | <tr> |
| + | <td>By decorating the peroxisomes with the v-SNARE Snc1 we successfully <a href="https://2017.igem.org/Team:Cologne-Duesseldorf/Results#Secretion">secreted</a> their entire contents</td> |
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| + | </tr> |
| | | |
| + | <tr> |
| + | <td>With two different <a href="https://2017.igem.org/Team:Cologne-Duesseldorf/Results#Sensors">sensors</a> we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes</td> |
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| + | </tr> |
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− | <table>
| + | <tr> |
− | <tbody>
| + | <td>Via fluorescence microscopy we verified that the <a href="https://2017.igem.org/Team:Cologne-Duesseldorf/Results#MembraneIntegration">integration of new membrane proteins</a> into the peroxisomal membrane is possible</td> |
− | <tr>
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
− | <td><div><a href="https://2017.igem.org/Team:Aix-Marseille ">MAX MUSTERMANN</a> align="right"</div></td>
| + | </tr> |
− | <td>SCHEIßE</td>
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− | <td>SCHEIßE2</td>
| + | |
− | </tr>
| + | |
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− | <tr>
| + | <tr> |
− | <td><a href="https://2017.igem.org/Team:Aachen">Aachen</a></td>
| + | <td>By successfully translocating the required enzymes for the metabolic pathways of <a href="https://2017.igem.org/Team:Cologne-Duesseldorf/Results#Nootkatone">Nootkatone</a> and <a href="https://2017.igem.org/Team:Cologne-Duesseldorf/Results#Violacein">Violacein</a> into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox</td> |
− | <td><a href="https://2017.igem.org/Team:Aalto-Helsinki/Collaborations">Aalto-Helsinki</a></td>
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
− | <td><a href="https://2017.igem.org/Team:Aix-Marseille ">Aix-Marseille</a></td>
| + | </tr> |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="https://2017.igem.org/Team:Bielefeld-CeBiTec ">Bielefeld-CeBiTec</a></td>
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− | <td><a href="https://2017.igem.org/Team:UBonn_HBRS ">UBonn_HBRS</a></td>
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− | <td><a href="https://2017.igem.org/Team:ColumbiaNYC">ColumbiaNYC</a></td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </table>
| + | |
| | | |
| + | <tr> |
| + | <td>We successfully implemented a way of customizing the <a href="https://2017.igem.org/Team:Cologne-Duesseldorf/Results#SizeAndNumber">size and number</a> of the peroxisomes into our toolbox</td> |
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| + | </tr> |
| | | |
| + | <tr> |
| + | <td>With a high throughput assay we characterized the import efficiency of <a href="https://2017.igem.org/Team:Cologne-Duesseldorf/Results#Pex7Import">different PTS2 sequences</a></td> |
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| + | </tr> |
| | | |
− | <img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left"> | + | <tr> |
| + | <td>To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively <a href="https://2017.igem.org/Team:Cologne-Duesseldorf/Model">modeled</a> the whole nootkatone pathway and the benefits of it being translocated inside our compartment</td> |
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| + | </tr> |
| | | |
| + | <tr> |
| + | <td>For our planned optogenetic experiments we designed an affordable <a href="https://2017.igem.org/Team:Cologne-Duesseldorf/Hardware">lightbox</a> which can easily be assembled in a short time |
| + | </td> |
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
| + | </tr> |
| | | |
− | <table> | + | <tr> |
− | <tbody> | + | <td>All our excellent <a href="https://2017.igem.org/Team:Cologne-Duesseldorf/Results">results</a> can be combined into a highly variable compartment <strong>toolbox</strong> for designing artificial compartments based on the peroxisomes in <i>S. cerevisiae</i> with an enormous range of applications |
− | <tr>
| + | </td> |
− | <td>HIER DEN TEXT</td>
| + | <td><img src="https://static.igem.org/mediawiki/2017/4/46/T--Cologne-Duesseldorf--check.png" width="100" height="100"></td> |
− | <td><div align="right">
| + | </tr> |
− | <img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;">
| + | |
− | </div>
| + | |
− | </td>
| + | |
− | </tr>
| + | |
− | | + | |
− | </tbody>
| + | |
− | </table>
| + | |
− | | + | |
| | | |
| | | |
− | | + | </tbody> |
− | | + | </table> |
− | <div class="callout">
| + | |
− | <h2>Results and engineered constructs of artico</h2>
| + | |
− | <ul>
| + | |
− | <li><img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg">We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in <i>S. cerevisiae</i>.
| + | |
− | </li>
| + | |
− | <li>By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents
| + | |
− | </li>
| + | |
− | <li>With two different sensors we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes.
| + | |
− | </li>
| + | |
− | <li>Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible.
| + | |
− | </li>
| + | |
− | <li>By successfully translocating the required enzymes for the metabolic pathways of Nootkatone and Violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox
| + | |
− | </li>
| + | |
− | <li>We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox.
| + | |
− | </li>
| + | |
− | <li>With a high throughput assay we characterized the import efficiency of different PTS2 sequences.
| + | |
− | </li>
| + | |
− | <li>To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment.
| + | |
− | </li>
| + | |
− | <li>For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time.
| + | |
− | </li>
| + | |
− | <li>All our excellent results can be combined into a highly variable compartment toolbox for designing artificial compartments based on the peroxisomes in <i>S. cerevisiae</i> with an enormous range of applications.
| + | |
− | </li> | + | |
− | </ul> | + | |
| </div> | | </div> |
| | | |