Difference between revisions of "Team:UCC Ireland"
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| − | <center style="font-size:24px;font-weight:500;"> | + | <center style="font-size:24px;font-weight:500;">Bioengineered construct</center> |
| − | + | A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein. | |
| + | <img class="homepage_into_img" src='https://static.igem.org/mediawiki/2017/b/b2/T--UCC_Ireland--amilcp.png'> | ||
</div> | </div> | ||
</div> | </div> | ||
<div class='abstract_section'> | <div class='abstract_section'> | ||
<div class='abstract_tile'> | <div class='abstract_tile'> | ||
| − | <center style="font-size:24px;font-weight:500;"> | + | <center style="font-size:24px;font-weight:500;">Chassis</center> |
2. Chassis | 2. Chassis | ||
The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system). | The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system). | ||
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<div class='abstract_tile'> | <div class='abstract_tile'> | ||
| − | <center style="font-size:24px;font-weight:500;"> | + | <center style="font-size:24px;font-weight:500;">Readout device</center> |
3. Readout Device | 3. Readout Device | ||
The readout device has been 3D printed and is designed to attach to common smartphones. The device acts as a spectrophotometer, enabling easy measurement of the relative fluorescence or absorbance of a sample. Using a mobile app and algorithms developed by the team, the device compares the fluorescence/absorbance measurement to a standard curve and determine the concentration of the substance being tested for in the sample. | The readout device has been 3D printed and is designed to attach to common smartphones. The device acts as a spectrophotometer, enabling easy measurement of the relative fluorescence or absorbance of a sample. Using a mobile app and algorithms developed by the team, the device compares the fluorescence/absorbance measurement to a standard curve and determine the concentration of the substance being tested for in the sample. | ||
Revision as of 21:31, 1 November 2017
Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements: (1) a bioengineered construct, (2) the chassis and (3) the readout device.
