Difference between revisions of "Team:UCC Ireland"

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A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
 
A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
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Revision as of 21:37, 1 November 2017

UCC iGEM 2017

Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements: (1) a bioengineered construct, (2) the chassis and (3) the readout device.

Bioengineered construct
A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
Chassis
2. Chassis The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system).
Readout device
3. Readout Device The readout device has been 3D printed and is designed to attach to common smartphones. The device acts as a spectrophotometer, enabling easy measurement of the relative fluorescence or absorbance of a sample. Using a mobile app and algorithms developed by the team, the device compares the fluorescence/absorbance measurement to a standard curve and determine the concentration of the substance being tested for in the sample.
To validate the idea for our Universal Biosensor Strategy, we identified irish industries with a need for such a product. We have targeted the dairy and microbrewery industries as proof of concept for our project, to test for antibiotic or methanol residue.