Results |
Achieved |
|---|---|
| We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for peroxisomes in S. cerevisiae |  |
| By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents | |
| With two different sensors we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes | |
| Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible | |
| By successfully translocating the required enzymes for the metabolic pathways of Nootkatone and Violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox | |
| We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox | |
| With a high throughput assay we characterized the import efficiency of different PTS2 sequences | |
| To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment | |
| For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time | |
| All our excellent results can be combined into a highly variable compartment toolbox for designing artificial compartments based on the peroxisomes in S. cerevisiae with an enormous range of applications | |
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Revision as of 21:38, 1 November 2017
- We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in S. cerevisiae.
- By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents
- With two different sensors we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes.
- Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible.
- By successfully translocating the required enzymes for the metabolic pathways of nootkatone and violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox
- We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox.
- With a high throughput assay we characterized the import efficiency of different PTS2 sequences.
- To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment.
- For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time.
