Difference between revisions of "Team:ITB Indonesia/Improve"
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<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color: #1c2922">Improve</h1> | <h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color: #1c2922">Improve</h1> | ||
| − | <p><a href='http://parts.igem.org/Part:BBa_K2378007'>BBa_K2378007</a> is an improved coding sequence of IrrE from | + | <p><a href='http://parts.igem.org/Part:BBa_K2378007'>BBa_K2378007</a> is an improved coding sequence of IrrE from <a href='http://parts.igem.org/Part:BBa_K729001>BBa_K729001</a>by Team UCL London 2012. When transformed to E. coli, this protein protected from salt, oxidative, and thermal shock. |
<i>Background of Improvement</i> | <i>Background of Improvement</i> | ||
Revision as of 21:59, 1 November 2017
Improve
Improve
BBa_K2378007 is an improved coding sequence of IrrE from [1]
From the alignment process, it is shown that the 'lost' protein from the stop codon is not from the peptidase domain sequence. But, from the length of the untranslated residues (~30 aa), it is hypothesized that this will affect the structural stability of the protein itself. Moreover, the second protein is not functional and does not have any motifs, hence will be possible burden for the metabolic process efficiency.
Improvement
We optimized the sequence for the expression in Escherichia coli BL21 by using synonymous mutations, then made the protein contiguous while being translated. We also removed the low complexity region.
