Difference between revisions of "Team:Stuttgart/HP/Silver"

 
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{{Template:Home}}
 
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        $("#HQ_page").attr('id','');
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<!-- start of content -->
 
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<h1 align=middle> Silver HP </h1>
        // call the functions that control the menu
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<div class="container">
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     <div class="row section">
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         <div class="col-xs-12 col-sm-12 col-md-12">
 
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          <p> We wanted to get more information about the local tube cleaning processes and issues. Therefore the iGEM-Team Stuttgart interviewed the tube cleaner company Rohr-Fuchs (abbreviated RF in the interview), what can be translated as pipe-fox, on the reasons of clogged drains and on their opinion on our genetically modified biological tube cleanser and the alternative using chemical tube cleaners.
 
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<div style="text-align: center;"><img src="https://static.igem.org/mediawiki/2017/f/f8/Stgt-rffuhr.jpeg"/></div>
 
+
</div>
        //this function controls the expand and collapse buttons of the menu and changes the +/- symbols
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        function menu_functionality() {
+
<div class="row section">
 
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            //when clicking on a "menu_button", it will change the "+/-" accordingly and it will show/hide the corresponding submenu
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<u>iGEM-Team:</u><p>What is your first idea when you hear the word genetic engineering. Do you connect positive or negative associations with this term?</p>
            $(".menu_button").click(function(){
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<u>RF:</u><p> My first thought is about external influence on DNA. Generally, artificial interferences with complex biological systems are negative connoted due to the difficult predictability. However, if this technique is responsibly used there may be great opportunities.
 
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</p>
                // add or remove the class "open" , this class holds the "-"
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<u>iGEM-Team:</u><p> What is generally your opinion on our biological tube cleaner which is based on a holistic approach using <i>E.coli</i>?</p>
                $(this).children().toggleClass("open");
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<u>RF:</u> <p>Generally, this idea seems to be laudable, nevertheless, our experiences on working with lipases showed that applied microorganisms can be a thread to other organisms. Similar experiments led to breakdowns of sewage treatment plants in the past. That is why we remain skeptical about the benefits of such a biological tube cleaner so far.
                // show or hide the submenu
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</p>
                $(this).next('.submenu_wrapper').fadeToggle(400);
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<u>iGEM-Team:</u><p> Is it possible that chemical tube cleaners cause environmental problems?</p>
            });
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<u>RF:</u><p> We are convinced of that. Aggressive and small molecular components are difficult to neutralize. Furthermore, sewage treatment plants work with microorganisms as well, which have to struggle especially with chemicals in waste water. Another aspect would be that these aggressive chemicals corrode the sewage pipes, damaging them what is followed by the release of such chemicals into the ground water.
 
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            // when the screen size is smaller than 800px, the display_menu_control button appears and will show/hide the whole menu
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+
                $('#menu_content').fadeToggle(400);
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+
 
+
            // call the current page highlight function
+
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+
 
+
 
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        // call the highlight current page function to show it on the menu with a different background color
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+
 
+
            // select a page from the menu based on the id assigned to it and the current page name and add the class "current page" to make it change background color
+
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+
 
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            // now that the current_page class has been added to a menu item, make the submenu fade in
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            // change the +/- symbol of the corresponding menu button
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            $(".current_page").parents(".submenu_wrapper").prev().children().toggleClass("open");
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        // allow button on the black menu bar to show/hide the side menu
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            // in case you preview mode is selected, the menu is hidden for better visibility
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            if (window.location.href.indexOf("submit") >= 0) {
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                $(".igem_2017_menu_wrapper").hide();
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            // if the black menu bar has been loaded
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            if (document.getElementById('bars_item')) {
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                // when the "bars_item" has been clicked
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                $("#bars_item").click(function() {
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                    $("#sideMenu").hide();
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                    // show/hide the menu wrapper
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</script>
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<style>
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    /***************************************************** DEFAULT WIKI SETTINGS  ****************************************************/
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    /* Clear the default wiki settings */
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    #home_logo, #sideMenu { display:none; }
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    #sideMenu, #top_title, .patrollink  {display:none;}
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    #content { width:100%; padding:0px;  margin-top:-7px; margin-left:0px;}
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    body {background:#190707 }
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    #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
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    /* wrapper for the submenu items, they are hidden by default*/
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    /* when the page size is bigger than 800px, this show/hide control is hidden by default */
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    /***************************************************** CONTENT OF THE PAGE ****************************************************/
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    /* Wrapper for the content */
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    /********************************* HTML STYLING  *********************************/
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    /* styling for the titles h1 h2 */
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    .igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 {
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        color:#F8CE63;
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    /* styling for the titles  h3 h4 h5 h6*/
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    .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 {
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        padding:5px 15px;
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        border-bottom:0;
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    /* font and text */
+
    .igem_2017_content_wrapper p {
+
        padding: 15px 0;
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        font-size: 16px;
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    .igem_2017_content_wrapper img {
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        padding: 15px 0;
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    /* Links */
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        font-weight: bold;
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        text-decoration: underline;
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        text-decoration-color: #190707;
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        -webkit-transition: all 0.4s ease;
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        -moz-transition: all 0.4s ease;
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        -ms-transition: all 0.4s ease;
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        -o-transition: all 0.4s ease;
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        transition: all 0.4s ease;
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    /* hover for the links */
+
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+
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+
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+
 
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+
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        font-family:Tahoma, Geneva, sans-serif;
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+
 
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    /* numbered lists */
+
    .igem_2017_content_wrapper ol {
+
        padding:0px;
+
        font-size: 15px;
+
        font-family:Tahoma, Geneva, sans-serif;
+
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+
 
+
    /* Table */
+
    .igem_2017_content_wrapper table {
+
        width: 97%;
+
        margin:15px 10px;
+
        border: 2px solid #190707;
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        border-collapse: collapse;
+
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+
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+
 
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    /* table cells */
+
    .igem_2017_content_wrapper  td {
+
        padding: 10px;
+
        vertical-align: text-top;
+
        border: 1px solid #190707;
+
        border-collapse: collapse;
+
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+
 
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    /* table headers */
+
    .igem_2017_content_wrapper th {
+
        padding: 10px;
+
        vertical-align: text-top;
+
        border: 1px solid #190707;
+
        border-collapse: collapse;
+
       
+
    }
+
 
+
 
+
    /**********************************LAYOUT CLASSES **********************************/
+
 
+
    /* general class for column divs */
+
    .igem_2017_content_wrapper .column  {
+
        padding: 10px 0px;
+
        float:left;
+
    }
+
 
+
    /* class for a full width column */
+
    .column .full_size {
+
        width:100%;
+
    }
+
 
+
    /* styling for images in a full width column*/
+
    .column.full_size img {
+
        width:97%;
+
        padding: 10px 15px;
+
    }
+
 
+
    /* class for a half width column */
+
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+
        width: 50%;
+
    }
+
    /* styling for images in a half width column*/
+
    .column.half_size img {
+
        width: 94.5%;
+
        padding: 10px 15px;
+
    }
+
 
+
 
+
 
+
 
+
    /********************************* SUPPORT CLASSES ********************************/
+
 
+
    /* class that clears content below*/
+
    .igem_2017_content_wrapper .clear {
+
        clear:both;
+
    }
+
 
+
 
+
    /* adds extra spacing when clearing content */
+
    .igem_2017_content_wrapper  .clear.extra_space {
+
        height: 30px;
+
    }
+
 
+
 
+
    /* highlight class, makes content slightly smaller */
+
    .igem_2017_content_wrapper .highlight {
+
        margin: 0px 15px;
+
        padding: 15px 0px;
+
    }
+
 
+
 
+
    /* highlight class, adds a gray background */
+
    .igem_2017_content_wrapper .highlight.gray {
+
        background-color: #190707;
+
    }
+
 
+
    /* highlight with decoration blue line on top */
+
    .igem_2017_content_wrapper .highlight.blue_top {
+
        border-top: 4px solid #190707;
+
    }
+
 
+
    /* highlight with a full blue border decoration */
+
    .igem_2017_content_wrapper .highlight.blue_border {
+
        border: 4px solid  #190707;
+
    }
+
 
+
 
+
    /* button class */
+
    .igem_2017_content_wrapper .button{
+
        max-width: 35%;
+
        margin: 30px auto;
+
        padding: 12px 10px;
+
        background-color: #190707;
+
        text-align: center;
+
        color: #F8CE63;
+
        -webkit-transition: all 0.4s ease;
+
        -moz-transition: all 0.4s ease;
+
        -ms-transition: all 0.4s ease;
+
        -o-transition: all 0.4s ease; transition: all 0.4s ease;
+
    }
+
 
+
    /* styling for button on hover */
+
    .igem_2017_content_wrapper .button:hover{
+
        background-color: #190707;
+
        color: #F8CE63;
+
    }
+
 
+
 
+
 
+
 
+
    /***************************************************** RESPONSIVE STYLING ****************************************************/
+
 
+
    /* IF THE SCREEN IS LESS THAN 1200PX */
+
    @media only screen and (max-width: 1200px) {
+
 
+
        #content {width:100%; }
+
        .igem_2017_menu_wrapper {width:15%; right:0;}
+
        .highlight {padding:10px 0px;}
+
        .igem_2017_menu_wrapper #display_menu_control { display:none; }
+
        #menu_content { display:block;}
+
        .menu_button.direct_to_page {padding-left: 17px;}
+
 
+
    }
+
 
+
    /* IF THE SCREEN IS LESS THAN 800PX */
+
    @media only screen and (max-width: 800px) {
+
 
+
        .igem_2017_menu_wrapper { width:100%; height: 15%; position:relative; left:0%;}
+
        .igem_2017_content_wrapper {width:100%; margin-left:0px;}
+
        .column.half_size  {width:100%; }
+
        .column.full_size img, .column.half_size img {  width: 100%; padding: 10px 0px;}
+
        .highlight {padding:15px 5px;}
+
        .igem_2017_menu_wrapper #display_menu_control { display:block; }
+
        #menu_content { display:none;}
+
        .igem_2017_menu_wrapper .menu_button .expand_collapse_icon { width: 5%; }
+
        .menu_bottom_padding {display:none;}
+
        .menu_button.direct_to_page { padding-left: 36px; }
+
    }
+
 
+
 
+
 
+
 
+
    /* special class that the system uses to make sure the team wants a page to be evaluated */
+
    .judges-will-not-evaluate {
+
        width: 96.6%;
+
        margin: 5px 15px;
+
        display: block;
+
        border: 4px solid #3399ff;
+
        font-weight: bold;
+
    }
+
 
+
</style>
+
 
+
 
+
<!--- THIS IS WHERE THE HTML BEGINS --->
+
 
+
<head>
+
 
+
    <!-- This tells the browser that your page is responsive -->
+
    <meta name="viewport" content="width=device-width, initial-scale=1">
+
 
+
</head>
+
 
+
 
+
<!--MENU-->
+
<div class="igem_2017_menu_wrapper" >
+
 
+
 
+
 
+
    <a href="https://2017.igem.org/Team:Stuttgart">
+
        <img src="https://static.igem.org/mediawiki/2017/4/45/Bildschirmfoto_2017-10-17_um_14.23.20.png">
+
    </a>
+
 
+
 
+
    <!-- this div is hidden by default and will only be displayed if the screen size is too small -->
+
    <div class="menu_button" id="display_menu_control">
+
        MENU
+
    </div>
+
 
+
    <div id="menu_content">
+
 
+
 
+
 
+
 
+
        <a href="https://2017.igem.org/Team:Stuttgart">
+
            <div class="menu_button direct_to_page">
+
                HOME
+
            </div>
+
        </a>
+
 
+
 
+
 
+
        <div class="menu_button">
+
            <div class="expand_collapse_icon">  </div> TEAM
+
        </div>
+
 
+
        <div class="submenu_wrapper" id="team_submenu">
+
 
+
            <a href="https://2017.igem.org/Team:Stuttgart/Team">
+
                <div class="submenu_button" id="Team_page">
+
                    Team
+
                </div>
+
            </a>
+
 
+
            <a href="https://2017.igem.org/Team:Stuttgart/Collaborations">
+
                <div class="submenu_button"  id="Collaborations_page">
+
                    Collaborations
+
                </div>
+
            </a>
+
 
+
 
+
        </div>
+
 
+
 
+
 
+
 
+
 
+
        <div class="menu_button">
+
            <div class="expand_collapse_icon">  </div> PROJECT
+
        </div>
+
 
+
        <!-- project submenu -->
+
        <div class="submenu_wrapper">
+
 
+
            <a href="https://2017.igem.org/Team:Stuttgart/Description">
+
                <div class="submenu_button"  id="Description_page">
+
                    Description
+
                </div>
+
            </a>
+
 
+
   
+
 
+
            <a href="https://2017.igem.org/Team:Stuttgart/Experiments">
+
                <div class="submenu_button"  id="Experiments_page">
+
                    Experiments
+
                </div>
+
            </a>
+
 
+
 
+
            <a href="https://2017.igem.org/Team:Stuttgart/Notebook">
+
                <div class="submenu_button"  id="Notebook_page">
+
                    Notebook
+
                </div>
+
            </a>
+
 
+
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 +
<u>iGEM-Team:</u><p>Do you think that chemical tube cleaners can be the cause of damages in the pipe systems?</p>
 +
<u>RF:</u><p> Definitely! Many pipes are made of plastic nowadays and consumers usually use these chemical substances only until the blockage is already there, although, the instructions of such agents usually recommend a prophylactic use. Consequently, these aggressive chemicals cannot flow off causing large quantities of these chemicals to stay in the pipe which get corroded then by the chemicals. Even our metal spirals get damaged by these chemical agents.
 +
</p>
 +
<u>iGEM-Team:</u><p> These chemical tube cleaners contain substances such as nitrate, sodium carbonate and aluminum components. Can these substances cause problems, as we already have – e.g. in case of the nitrate – to much nitrate in (German) ground water? What happens to the aluminum hydroxide which results from the aluminum components?</p>
 +
<u>RF:</u><p> Indeed, the additional nitrate is a problem for the ground water. Same applies for the other aggressive components of chemical tube cleaners.
 +
</p>
 +
<u>iGEM-Team:</u><p> What do you think is the biggest problem in case of drain cleaning? Which substances, materials or blockages are a risk?
 +
<u>RF:</u><p> As described before we do not recommend corrosive liquid cleaner. Especially, tube cleaner in granule form should not be used as it accumulates in front of the blockage, swells and gets hard as concrete. Consequently, these substances are counterproductive and even complicate our work then. Blockages are often caused by non-soluble textiles which should be disposed in waste containers and never in the toilet. Furthermore, thin objects such as cotton swabs constitute a big burden for our sewage treatment plants as they are too thin to be removed by the filter system. If large amounts of fat get in to the drain system and it is not rinsed or only rinsed with cold water, fat accumulates till the pipe is totally clogged. Generally, always rinse with lots of water in case of removing any solid materials in the sink.
 +
</p>
 +
<u>iGEM-Team:</u><p> What do you think about the commercial biological tube cleaners. Are they efficient?</p>
 +
<u>RF:</u><p> Generally, we strongly advise against all tube cleaners due to environmental reasons, as they are an additional burden for the sewage treatment plants and the ground water.</p>
  
 +
<u>iGEM-Team:</u><p> How do you proceed in the drain cleaning? Can you explain the typical drain cleaning process?</p>
 +
<u>RF:</u><p> After a local inspection we remove the siphons of the sinks or the toilette. Then spirals made of metal are inserted in to the pipe to electromechanically mill it. Thereby the blockage gets solved and is transported to the next bigger pipe where it can flow of more easily. Lots of water while milling and afterwards helps to remove the blockage as well as its cause.
  
 +
<u>iGEM-Team:</u><p> Who needs your service more often, industry or households? And what does mostly cause the blockages?</p>
 +
<u>RF:</u><p> Industry and households call us equally. Only the causes are different: In households, the blockages are mostly caused by hair and soap while we have to fight with fat clogged pipes in restaurants. Feminine textile products are the main cause for blockages in public toilettes and clogged pipes in the industry are mostly caused due to wrong construction work as the pipes are not made for the increased usage.
 +
</p>
 +
<u>iGEM-Team:</u><p> It seems like that blockages caused by fat occur more often recently. How do you unclog such blockages? Could lipases be helpful?</p>
 +
<u>RF:</u><p> It is laudable that households use less water however you should not save hot water especially in the kitchen. We often have cases in which the kitchen pipes are totally blocked by fat. Here helps only lots of warm water and patience. We cannot recommend the usage of lipases or any other chemical substances.</p>
  
 +
<u>iGEM-Team:</u><p> Does human hair constitute a problem in pipes?</p>
 +
<u>RF:</u><p> Indeed! Especially, if people complain about clogged shower or bath tub sinks we can be almost sure that hair is the main cause in these blockages.</p>
 +
<u>iGEM-Team:</u><p> Would it be an advantage for the consumer if there is a fragrance produced while degrading the hair.</p>
 +
<u>RF:</u><p> As long as these vapors are not harmful it can be a pleasant side effect.
 +
<br>
 +
<u>iGEM-Team:</u><p> Is it possible that genetically engineered E. coli pose problems in the drain? Do you have problems with biofilms when cleaning pipes? If yes, how do you fight these biofilms?</p>
 +
<u>RF:</u><p> No, we do not have such problems.
 +
</p>
 +
<u>iGEM-Team:</u> Do you know if GVOs are used to clean drains? If yes, in which way?</p>
 +
<u>RF:</u><p> No, as our area of expertise is the mechanical cleaning.</p>
 +
<u>iGEM-Team:</u><p> If we can make sure that the E. colis kill themselves in the drain and only enzymes such as keratinases, lipases and esterases stay in the pipe would you use the microbial tube cleaner?</p>
 +
<u>RF:</u><p> If these enzymes do not attack other organisms the usage of such a tube cleaner seems to be okay. However, our area of expertise is the mechanical cleaning as this method demonstrably does not burden the sewage in any way.</p>
 +
<u>iGEM-Team:</u><p> What would you like to tell consumers for a preventive drain cleaning? Which tips can you give to prevent blockages?</p>
 +
<u>RF:</u><p> Do not throw any materials into your sinks which are not soluble in water. Even if they are so small that they go through the sieve, rinse thoroughly! In case of fat, use warm water for rinsing. Although toilet drains have a bigger diameter feminine hygiene products cannot be fully removed, consequently, throw these products into waste containers.</p>
 
</div>
 
</div>
 
 
<!-- start of content -->
 
<div class="igem_2017_content_wrapper">
 
<h1 align=middle>HP silver</h1>
 
<table>
 
<td bgcolor=#F8CE63>
 
 
<div class="column full_size judges-will-not-evaluate">
 
<h3>★  ALERT! </h3>
 
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
 
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
 
</div>
 
</div>
<div class="clear"></div>
+
<div class="row section">
 
+
<div style="text-align: center;"><a href="http://www.rohr-fuchs-service.com/"><img src="text-align: center;"><img src="https://static.igem.org/mediawiki/2017/7/78/Stgt_rohrfuchs.jpeg" alt="Bild" /></a>
 
+
<div class="column full_size">
+
 
+
<h1>Silver Medal Human Practices</h1>
+
<p>iGEM teams are leading in the area of Human Practices because they conduct their projects within a social/environmental context, to better understand issues that might influence the design and use of their technologies.</p>
+
<p>Teams work with students and advisors from the humanities and social sciences to explore topics concerning ethical, legal, social, economic, safety or security issues related to their work. Consideration of these Human Practices is crucial for building safe and sustainable projects that serve the public interest. </p>
+
<p>For more information, please see the <a href="https://2017.igem.org/Competition/Human_Practices">Human Practices page</a>.</p>
+
 
</div>
 
</div>
 
<div class="clear"></div>
 
 
<div class="column half_size">
 
<h3>Silver Medal Criterion #3</h3>
 
<p>Convince the judges you have thought carefully and creatively about whether your work is safe, responsible and good for the world. You could accomplish this through engaging with your local, national and/or international communities or other approaches. Please note that standard surveys will not fulfill this criteria.</p>
 
 
</div>
 
</div>
 
<div class="column half_size">
 
<h5>Some Human Practices topic areas </h5>
 
<ul>
 
<li>Philosophy</li>
 
<li>Public Engagement / Dialogue</li>
 
<li>Education</li>
 
<li>Product Design</li>
 
<li>Scale-Up and Deployment Issues</li>
 
<li>Environmental Impact</li>
 
<li>Ethics</li>
 
<li>Safety</li>
 
<li>Security</li>
 
<li>Public Policy</li>
 
<li>Law and Regulation</li>
 
<li>Risk Assessment</li>
 
</ul>
 
 
</div>
 
</div>
  
  
<div class="column half_size">
+
               
<h5>What should we write about on this page?</h5>
+
<p>On this page, you should write about the Human Practices topics you considered in your project, and document any special activities you did (such as visiting experts, talking to lawmakers, or doing public engagement). This should include all of the work done for the Silver Medal Criterion #3. Details for your Gold medal work and/or work for the two Human Practices special prizes should be put on those specified pages.</p>
+
</div>
+
  
  
<div class="column half_size">
 
<h5>Inspiration</h5>
 
<p>Read what other teams have done:</p>
 
<ul>
 
<li><a href="https://2014.igem.org/Team:Dundee/policypractice/experts">2014 Dundee </a></li>
 
<li><a href="https://2014.igem.org/Team:UC_Davis/Policy_Practices_Overview">2014 UC Davis </a></li>
 
<li><a href="https://2013.igem.org/Team:Manchester/HumanPractices">2013 Manchester </a></li>
 
<li><a href="https://2013.igem.org/Team:Cornell/outreach">2013 Cornell </a></li>
 
</ul>
 
</div>
 
 
</td>
 
</table>
 
  
 
</html>
 
</html>

Latest revision as of 22:08, 1 November 2017

Silver HP

We wanted to get more information about the local tube cleaning processes and issues. Therefore the iGEM-Team Stuttgart interviewed the tube cleaner company Rohr-Fuchs (abbreviated RF in the interview), what can be translated as pipe-fox, on the reasons of clogged drains and on their opinion on our genetically modified biological tube cleanser and the alternative using chemical tube cleaners.

iGEM-Team:

What is your first idea when you hear the word genetic engineering. Do you connect positive or negative associations with this term?

RF:

My first thought is about external influence on DNA. Generally, artificial interferences with complex biological systems are negative connoted due to the difficult predictability. However, if this technique is responsibly used there may be great opportunities.

iGEM-Team:

What is generally your opinion on our biological tube cleaner which is based on a holistic approach using E.coli?

RF:

Generally, this idea seems to be laudable, nevertheless, our experiences on working with lipases showed that applied microorganisms can be a thread to other organisms. Similar experiments led to breakdowns of sewage treatment plants in the past. That is why we remain skeptical about the benefits of such a biological tube cleaner so far.

iGEM-Team:

Is it possible that chemical tube cleaners cause environmental problems?

RF:

We are convinced of that. Aggressive and small molecular components are difficult to neutralize. Furthermore, sewage treatment plants work with microorganisms as well, which have to struggle especially with chemicals in waste water. Another aspect would be that these aggressive chemicals corrode the sewage pipes, damaging them what is followed by the release of such chemicals into the ground water. iGEM-Team:

Do you think that chemical tube cleaners can be the cause of damages in the pipe systems?

RF:

Definitely! Many pipes are made of plastic nowadays and consumers usually use these chemical substances only until the blockage is already there, although, the instructions of such agents usually recommend a prophylactic use. Consequently, these aggressive chemicals cannot flow off causing large quantities of these chemicals to stay in the pipe which get corroded then by the chemicals. Even our metal spirals get damaged by these chemical agents.

iGEM-Team:

These chemical tube cleaners contain substances such as nitrate, sodium carbonate and aluminum components. Can these substances cause problems, as we already have – e.g. in case of the nitrate – to much nitrate in (German) ground water? What happens to the aluminum hydroxide which results from the aluminum components?

RF:

Indeed, the additional nitrate is a problem for the ground water. Same applies for the other aggressive components of chemical tube cleaners.

iGEM-Team:

What do you think is the biggest problem in case of drain cleaning? Which substances, materials or blockages are a risk? RF:

As described before we do not recommend corrosive liquid cleaner. Especially, tube cleaner in granule form should not be used as it accumulates in front of the blockage, swells and gets hard as concrete. Consequently, these substances are counterproductive and even complicate our work then. Blockages are often caused by non-soluble textiles which should be disposed in waste containers and never in the toilet. Furthermore, thin objects such as cotton swabs constitute a big burden for our sewage treatment plants as they are too thin to be removed by the filter system. If large amounts of fat get in to the drain system and it is not rinsed or only rinsed with cold water, fat accumulates till the pipe is totally clogged. Generally, always rinse with lots of water in case of removing any solid materials in the sink.

iGEM-Team:

What do you think about the commercial biological tube cleaners. Are they efficient?

RF:

Generally, we strongly advise against all tube cleaners due to environmental reasons, as they are an additional burden for the sewage treatment plants and the ground water.

iGEM-Team:

How do you proceed in the drain cleaning? Can you explain the typical drain cleaning process?

RF:

After a local inspection we remove the siphons of the sinks or the toilette. Then spirals made of metal are inserted in to the pipe to electromechanically mill it. Thereby the blockage gets solved and is transported to the next bigger pipe where it can flow of more easily. Lots of water while milling and afterwards helps to remove the blockage as well as its cause. iGEM-Team:

Who needs your service more often, industry or households? And what does mostly cause the blockages?

RF:

Industry and households call us equally. Only the causes are different: In households, the blockages are mostly caused by hair and soap while we have to fight with fat clogged pipes in restaurants. Feminine textile products are the main cause for blockages in public toilettes and clogged pipes in the industry are mostly caused due to wrong construction work as the pipes are not made for the increased usage.

iGEM-Team:

It seems like that blockages caused by fat occur more often recently. How do you unclog such blockages? Could lipases be helpful?

RF:

It is laudable that households use less water however you should not save hot water especially in the kitchen. We often have cases in which the kitchen pipes are totally blocked by fat. Here helps only lots of warm water and patience. We cannot recommend the usage of lipases or any other chemical substances.

iGEM-Team:

Does human hair constitute a problem in pipes?

RF:

Indeed! Especially, if people complain about clogged shower or bath tub sinks we can be almost sure that hair is the main cause in these blockages.

iGEM-Team:

Would it be an advantage for the consumer if there is a fragrance produced while degrading the hair.

RF:

As long as these vapors are not harmful it can be a pleasant side effect.
iGEM-Team:

Is it possible that genetically engineered E. coli pose problems in the drain? Do you have problems with biofilms when cleaning pipes? If yes, how do you fight these biofilms?

RF:

No, we do not have such problems.

iGEM-Team: Do you know if GVOs are used to clean drains? If yes, in which way?

RF:

No, as our area of expertise is the mechanical cleaning.

iGEM-Team:

If we can make sure that the E. colis kill themselves in the drain and only enzymes such as keratinases, lipases and esterases stay in the pipe would you use the microbial tube cleaner?

RF:

If these enzymes do not attack other organisms the usage of such a tube cleaner seems to be okay. However, our area of expertise is the mechanical cleaning as this method demonstrably does not burden the sewage in any way.

iGEM-Team:

What would you like to tell consumers for a preventive drain cleaning? Which tips can you give to prevent blockages?

RF:

Do not throw any materials into your sinks which are not soluble in water. Even if they are so small that they go through the sieve, rinse thoroughly! In case of fat, use warm water for rinsing. Although toilet drains have a bigger diameter feminine hygiene products cannot be fully removed, consequently, throw these products into waste containers.

Bild