Difference between revisions of "Team:UCC Ireland"

 
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<p id='home_page'> Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements: (1) a bioengineered construct, (2) the chassis and (3) the readout device.
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<p id='home_page'> Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements:  
 
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<center style="font-size:24px;font-weight:700;">Bioengineered construct</center>
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<center style="font-size:24px;font-weight:700;padding-bottom:5px;padding-top:0px;">Bioengineered construct</center>
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A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
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<img class="homepage_into_img" src='https://static.igem.org/mediawiki/2017/b/b2/T--UCC_Ireland--amilcp.png'>
 
<img class="homepage_into_img" src='https://static.igem.org/mediawiki/2017/b/b2/T--UCC_Ireland--amilcp.png'>
 
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A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
 
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<center style="font-size:24px;font-weight:500;">Chassis</center>
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<center style="font-size:24px;font-weight:700;padding-bottom:5px;padding-top:0px;">Chassis</center>
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2. Chassis
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The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system).
 
The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system).
 
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<center style="font-size:24px;font-weight:500;">Readout device</center>
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<center style="font-size:24px;font-weight:700;padding-bottom:5px;padding-top:0px;">Readout device</center>
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3. Readout Device
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The readout device has been 3D printed and is designed to attach to common smartphones. The device acts as a spectrophotometer, enabling easy measurement of the relative fluorescence or absorbance of a sample. Using a mobile app and algorithms developed by the team, the device compares the fluorescence/absorbance measurement to a standard curve and determine the concentration of the substance being tested for in the sample.
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<img class="homepage_into_img" src='https://static.igem.org/mediawiki/2017/9/91/T--UCC_Ireland--phoneclipart.png'>
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The readout device was 3D printed and designed to attach easily to common smartphones. It uses spectrometry technology to measure the relative fluorescence or absorbance of a sample. We built an app to analyse the output from the device to determine the concentration of the substance being tested for.
 
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To validate the idea for our Universal Biosensor Strategy, we identified irish industries with a need for such a product. We have targeted the dairy and microbrewery industries as proof of concept for our project, to test for antibiotic or methanol residue. </p>
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To validate the idea for our Universal Biosensor Strategy, we identified irish industries with a need for such a product. We have targeted the dairy and microbrewery industries as proof of concept for our project, to test for antibiotic or methanol residue.  
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<img src='https://static.igem.org/mediawiki/2017/5/58/T--UCC_Ireland--methanol_home_page.jpg' style='width: 90%; margin: auto; display: block;'></img>
 
<img src='https://static.igem.org/mediawiki/2017/5/58/T--UCC_Ireland--methanol_home_page.jpg' style='width: 90%; margin: auto; display: block;'></img>

Latest revision as of 23:02, 1 November 2017

UCC iGEM 2017

Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements:

Bioengineered construct
A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
Chassis
The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system).
Readout device
The readout device was 3D printed and designed to attach easily to common smartphones. It uses spectrometry technology to measure the relative fluorescence or absorbance of a sample. We built an app to analyse the output from the device to determine the concentration of the substance being tested for.
To validate the idea for our Universal Biosensor Strategy, we identified irish industries with a need for such a product. We have targeted the dairy and microbrewery industries as proof of concept for our project, to test for antibiotic or methanol residue.