Difference between revisions of "Team:CCA San Diego/Improve"

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<h3>★  ALERT! </h3>
 
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
 
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
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<center><h2 style="/*position:center;*//*position:center;*/text-align:center;position:1000px;padding-top:50px;font-size:79px;color:#56cfff;">improvement</h2>
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<h1>Improve</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
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<h3>Gold Medal Criterion #2</h3>
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<center><table><tr><td><partinfo><h6 style="font-size:35px"><a href="http://parts.igem.org/Part:pSB3K3" target="_blank">pSB3K3</a> &rarr; <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2491030" target="_blank">BBa_K2491030</h6></partinfo></td></tr></table></center>
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
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<h6>The goal of this improvement was to replace p15a origin of replication in pSB3K3 with the RK2 ori to generate a shuttle vector as a broad host range vector (i.e. can replicate in E.coli and other microorganisms).
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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<br><br>The RK2 origin of replication is a broad-host-range plasmid belonging to the incP incompatibility group that can be maintained in a large number of bacteria. The minimal region for replication and maintenance consists of an origin of replication, oriV, and the plasmid replication initiator protein TrfA protein, that activates oriV. The copy number of RK2 is about 4-7 per cell in E. coli, 3 in Pseudomonas aeruginosa, and 4-7 in Agrobacterium.
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<br><br>By applying this change, many teams may now have a broad range, low-copy system for a two-plasmid system within a wide variety of bacterial strains. In future application, our polycistronic degradation plasmids may be applied to broad-host-range remediation applications depending on the condition with which the water being treated is in. Applying our two-plasmid system to a wide variety of strains, will introduce the possibility of increased efficiency for PAH degradation in many environmental situations. Some bacterial strains may also code for many more pathways that can be combined with our own in future biodegradation applications. The application, through our improvement of pSB3K3, of two broad-host-range plasmids serve to aid in the controlling unwanted bacterial byproducts by improving the ability to select expression in specific strains, to allow for selection of optimal bacteria in field situations, and to reduce the use of harmful bacterial strains that contain many of the pathways we isolated.  
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<center><img src="https://static.igem.org/mediawiki/2017/3/39/Igem_plasmid.png" width="40%"></center>
  
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<h6>For more information please see our <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2491030" target="_blank">BBa_K2491030</a> part
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Latest revision as of 23:49, 1 November 2017

improvement

pSB3K3 → BBa_K2491030
The goal of this improvement was to replace p15a origin of replication in pSB3K3 with the RK2 ori to generate a shuttle vector as a broad host range vector (i.e. can replicate in E.coli and other microorganisms).

The RK2 origin of replication is a broad-host-range plasmid belonging to the incP incompatibility group that can be maintained in a large number of bacteria. The minimal region for replication and maintenance consists of an origin of replication, oriV, and the plasmid replication initiator protein TrfA protein, that activates oriV. The copy number of RK2 is about 4-7 per cell in E. coli, 3 in Pseudomonas aeruginosa, and 4-7 in Agrobacterium.

By applying this change, many teams may now have a broad range, low-copy system for a two-plasmid system within a wide variety of bacterial strains. In future application, our polycistronic degradation plasmids may be applied to broad-host-range remediation applications depending on the condition with which the water being treated is in. Applying our two-plasmid system to a wide variety of strains, will introduce the possibility of increased efficiency for PAH degradation in many environmental situations. Some bacterial strains may also code for many more pathways that can be combined with our own in future biodegradation applications. The application, through our improvement of pSB3K3, of two broad-host-range plasmids serve to aid in the controlling unwanted bacterial byproducts by improving the ability to select expression in specific strains, to allow for selection of optimal bacteria in field situations, and to reduce the use of harmful bacterial strains that contain many of the pathways we isolated.

For more information please see our BBa_K2491030 part

email igemcca@gmail.com
Canyon Crest Academy iGEM 2017 CC;