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Revision as of 01:11, 2 November 2017
![](https://static.igem.org/mediawiki/2017/7/73/T--Bielefeld-CeBiTec--start-page-screen1.png)
![](https://static.igem.org/mediawiki/2017/c/c4/T--Bielefeld-CeBiTec--start-page-screen2.png)
![](https://static.igem.org/mediawiki/2017/a/ad/T--Bielefeld-CeBiTec--start-page-screen3.png)
![](https://static.igem.org/mediawiki/2017/1/13/T--Bielefeld-CeBiTec--start-page-screen4.png)
![](https://static.igem.org/mediawiki/2017/0/01/T--Bielefeld-CeBiTec--start-page-screen5.png)
![](https://static.igem.org/mediawiki/2017/3/3b/T--Bielefeld-CeBiTec--start-page-screen6.png)
![](https://static.igem.org/mediawiki/2017/8/81/T--Bielefeld-CeBiTec--start-page-screen7.png)
![](https://static.igem.org/mediawiki/2017/b/b4/T--Bielefeld-CeBiTec--start-page-screen8.png)
Achievements
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Establishment of two orthogonal methods for the detection of unnatural base pairs in a target sequence via Oxford Nanopore sequencing and an enzyme based detection method
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of a software suite for these orthogonal methods
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Integration and characterization of the nucleotide transporter PtNTT2 from P.tricornutum in E.coli for the uptake of unnatural nucleoside triphosphates
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Proof that certain Taq-polymerases can efficiently incorporate unnatural nucleotides
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of a toolbox consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Colocalization of the RuBisCo using a fluorescent amino acid
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of a photoswitchable lycopene pathway
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Design, chemical synthesis and proof of functionality of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Modeling more than ten new aaRS sequences
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Library development with several hundred thousand sequences for selecting aminoacyl-tRNA synthetases
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of positive and negative selection plasmids for the evolution of new synthetases for non-canonical amino acids
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Improvement of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of an LED panel for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and much more
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of an Android App to control the LED panel with your smartphone via Bluetooth
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Writing a biosafety report titled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System”
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Writing the ChImp Report on “Chances and Implications of an Expanded Genetic Code”