Difference between revisions of "Team:UIOWA/Results"

 
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<p>Results and implications for Iowa iGEM 3HP biobrick construct</p>
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<img src="https://static.igem.org/mediawiki/2017/8/83/T--UIOWA--3HP_ASSAY.jpg" width=1000px style="padding-top: 2px;">
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<h5>This is a basic overview of the assay method that was used to determine our experimental results below</h5>
 
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<h5>You should also describe what your results mean: </h5>
 
  
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
 
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
 
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
 
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<h5> Project Achievements </h5>
 
  
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<img src="https://static.igem.org/mediawiki/2017/6/66/T--UIOWA--3HP_FIG1_FINAL.jpg" width=1000px style="padding-top: 2px;">
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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Our biobrick (mmsR/Pmmsa) is designed to luminesce, or emit off light, in the presence of 3HP. Induction of luminescence by 3HP is seen in Figure 2; all raw luminescence values divided by OD600 at then normalized to uninduced (0mM 3HP) values. However, induction only occurs when cells have been cultured in the presence of 3HP for 18 hours, and the growth curve in Figure 1 displays a dramatic lag in growth at 30mM and 40mM 3HP.
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Further investigation is needed to optimize the performance and design of our construct.
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<h5>Inspiration</h5>
 
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<p>See how other teams presented their results.</p>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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Latest revision as of 02:07, 2 November 2017


Results

Our biobrick (mmsR/Pmmsa) is designed to luminesce, or emit off light, in the presence of 3HP. Induction of luminescence by 3HP is seen in Figure 2; all raw luminescence values divided by OD600 at then normalized to uninduced (0mM 3HP) values. However, induction only occurs when cells have been cultured in the presence of 3HP for 18 hours, and the growth curve in Figure 1 displays a dramatic lag in growth at 30mM and 40mM 3HP. Further investigation is needed to optimize the performance and design of our construct.