Difference between revisions of "Team:GZHS-United"

 
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<h1>Project description</h1>
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<p>Bacillus thuringiensis israelensis (Bti) and  Bacillus sphaericus(BS)are  naturally existed bacterial that could be extracted from dirt, insect body, unclean water et al. Both of them are applied widely as biological larvicides over urban areas as they target specific insects and do not contaminate the environment. Cry protein family usually form during Bti spore-forming process. Cry4Ba is one of the studied crystal toxin protein that could combine with mosquito larvae midgut epithelia causing midgut to decay thus Aedes mosquito death. Mosquitocidal toxin 1 (Mtx1) protein form at BS trophophase stage. After eaten by mosquito larvae, Mtx protein would decomposed into peptided which leads to morphological change in mosquito larvae cells.  </p>
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<p>A variety of mixed toxic proteins could delay the emergence and development of insecticide resistance. BS is easy to make the target pests develop resistance, mainly due to single factor genetic resistance. Such resistance cases was already  reported in France, India, Brazil, China and other countries around the world.  Traditional insecticide resistance management strategies include rotation and blending of Bti with Bs, and new strategies include synthetic biology aim to expand toxin diversity against mosquito larvae to avoid the emergence of larvecide resistance. </p>
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<p>We believe that our research could ease the mosquito born disease burden  through genetically mix different mosquito toxin protein which not only add to the field of synthetic biology, but also help with larvecide resistance management through the implementation of scientific rigor to the process of creating new type to larvecide for insecticide industry. Our goal is to create a genetic modified mosquito larvae toxin and test for adaptation to mosquito breading sites in local environments.  The timeline above outlines major work done to achieve our goals. </p>
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<h3>GZHS-United</h3>
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<img src="https://static.igem.org/mediawiki/2017/e/ee/T--GZHS-United--home_Home-2.jpg" alt="">
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<h3>GZHS-United</h3>
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<h3>GZHS-United</h3>
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Entrepreneurship">Entrepreneurship</a>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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$(document).ready(function() {
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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$(window).scroll(function(){
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<div class="highlight">
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<div class="content">
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<h3>about us </h3>
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<a href="https://2017.igem.org/Team:GZHS-United/Team#Students">
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<img src="https://static.igem.org/mediawiki/2017/0/02/T--GZHS-United--home_Home-6.jpg" />
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<h2>Students</h2>
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<p>OUR STUDENTS</p>
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</a>
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<div class="view view-fourth view1" id="view2">
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<a href="https://2017.igem.org/Team:GZHS-United/Team#Introducers">
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<img src="https://static.igem.org/mediawiki/2017/c/cb/T--GZHS-United--home_Home-7.jpg" />
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<h2>Introducers</h2>
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<p>OUR INTRODUCERS</p>
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</a>
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<a href="https://2017.igem.org/Team:GZHS-United/Team#Advicors">
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<img src="https://static.igem.org/mediawiki/2017/a/ac/T--GZHS-United--home_Home-8.jpg" />
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<div class="mask">
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<h2>Advicors</h2>
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<p>OUR ADVICORS</p>
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</a>
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<h3> PROJECT ABSTRACT</h3>
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<img src="https://static.igem.org/mediawiki/2017/d/df/T--GZHS-United--home_mtx1.png" class="img-responsive" alt="" />
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<h5>MTX1</h5>
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<img src="https://static.igem.org/mediawiki/2017/e/e4/T--GZHS-United--home_gif.gif" class="img-responsive" alt="" />
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<a href="https://2017.igem.org/Team:GZHS-United/Design#Cry4Ba">
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<img src="https://static.igem.org/mediawiki/2017/8/81/T--GZHS-United--home_cry4ba.png" class="img-responsive" alt="" />
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<h5>Cry4Ba</h5>
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<h3>OUR WORK</h3>
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<p>Bs mainly targets Culex mosquitoes, while <em>Bt.i</em> exhibits strong killing effect against Aedes mosquitoes. However, if they work together in one system, the efficiency of both the two protein can improve significantly. Based on this information, we decided to build a system consisting these two proteins using synthetic biology. To ensure that we can get purified protein, we used <em>E.coli</em> as our bio-chasis to synthesize these proteins and applied them in toxicity test. For there had been many bio-bricks from <em>Cry</em> family in iGEM, the new part <em>Mtx1</em> was introduced into the biobricks in our project, which may be applied to strengthen the efficiency of former parts of <em>Cry</em>.</p>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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<a href="https://2017.igem.org/Special:Upload">
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UPLOAD FILES
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Latest revision as of 02:15, 2 November 2017

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PROJECT ABSTRACT

MTX1
Cry4Ba

OUR WORK

Bs mainly targets Culex mosquitoes, while Bt.i exhibits strong killing effect against Aedes mosquitoes. However, if they work together in one system, the efficiency of both the two protein can improve significantly. Based on this information, we decided to build a system consisting these two proteins using synthetic biology. To ensure that we can get purified protein, we used E.coli as our bio-chasis to synthesize these proteins and applied them in toxicity test. For there had been many bio-bricks from Cry family in iGEM, the new part Mtx1 was introduced into the biobricks in our project, which may be applied to strengthen the efficiency of former parts of Cry.