(2 intermediate revisions by one other user not shown) | |||
Line 46: | Line 46: | ||
<!-- Banner--> | <!-- Banner--> | ||
<div class = "row" style="margin-top:60px;padding:10px 0 0 0;background-color:#e2e2e2"> | <div class = "row" style="margin-top:60px;padding:10px 0 0 0;background-color:#e2e2e2"> | ||
− | <center><img class="crispy" src="https://static.igem.org/mediawiki/2017/9/93/T--Sydney_Australia--notebook_banner.png" class="img-center" width = " | + | <center><img class="crispy" src="https://static.igem.org/mediawiki/2017/9/93/T--Sydney_Australia--notebook_banner.png" class="img-center" width = "60%"></center> |
</div> | </div> | ||
<div class="row"> | <div class="row"> | ||
Line 447: | Line 447: | ||
<div> | <div> | ||
•Alex and Jamie digested our pSB1C3 stock and ligated the cytoplasmic and | •Alex and Jamie digested our pSB1C3 stock and ligated the cytoplasmic and | ||
− | Ecotin-tagged | + | Ecotin-tagged gBlocks into them. |
</div> | </div> | ||
<div> | <div> | ||
Line 516: | Line 516: | ||
</div> | </div> | ||
<div> | <div> | ||
− | •Steph transformed the pSB1C3 vectors with the YncM | + | •Steph transformed the pSB1C3 vectors with the YncM gBlocks inserted last |
week into TOP10 E.coli. These transformations did not work and we believe | week into TOP10 E.coli. These transformations did not work and we believe | ||
that it is due to a problem with the ligation of the gBlock into pSB1C3. | that it is due to a problem with the ligation of the gBlock into pSB1C3. | ||
Line 541: | Line 541: | ||
</p> | </p> | ||
<div> | <div> | ||
− | •Jamie redid the ligation of the YncM | + | •Jamie redid the ligation of the YncM gBlocks into pSB1C3 and transformed |
these vectors into TOP10 E.coli. | these vectors into TOP10 E.coli. | ||
</div> | </div> | ||
Line 610: | Line 610: | ||
•Now that it appears that all 9 gBlocks in pSB1C3 have been transformed | •Now that it appears that all 9 gBlocks in pSB1C3 have been transformed | ||
into TOP10 E.coli, Steph and Emaleen performed a colony screening PCR on a | into TOP10 E.coli, Steph and Emaleen performed a colony screening PCR on a | ||
− | few colonies of each transformation to verify presence of the | + | few colonies of each transformation to verify presence of the gBlock. |
− | + | ||
</div> | </div> | ||
<div> | <div> | ||
Line 1,227: | Line 1,226: | ||
</div> | </div> | ||
<div id="collapse10002" class="panel-collapse collapse"> | <div id="collapse10002" class="panel-collapse collapse"> | ||
− | <div class="panel-body">Day and night (what, what)<br>I toss and turn, I keep stressing my mind, mind (wiki, wiki)<br>I look for peace but see I don't attain (wiki, wiki)<br>What I need for keeps this silly game we play, | + | <div class="panel-body">Day and night (what, what)<br>I toss and turn, I keep stressing my mind, mind (wiki, wiki)<br>I look for peace but see I don't attain (wiki, wiki)<br>What I need for keeps this silly game we play, play<br></br>Now look at this (wiki, wiki)<br>Madness to magnet keeps attracting me, me (wiki, wiki)<br>I try to run but see I'm not that fast (wiki, wiki)<br>I think I'm first but surely finish last, last |
</div> | </div> | ||
</div> | </div> |
Latest revision as of 02:16, 2 November 2017
Project design:
Project design:
Wet Lab:
Project design:
Project design:
Education and Public Engagement:
Project design:
Human practices:
Project design:
Human practices:
Project design:
Wet lab:
Wet lab:
Human practices:
Wet lab:
This was the start of our winter break – however, the team took advantage of the break and planned to get a lot of lab work done.
Wet lab:
Wet lab:
Wet lab:
Wet Lab:
Wet lab:
Human practices:
Lab work:
Human practices:
Education and Public Engagement:
Wet lab:
Education and Public Engagement:
Human practices:
Wet Lab:
Education and public engagement:
Human practices:
Wet lab:
Human Practice:
Wet lab:
Education and Public Engagement:
Wet Lab:
Human Practices:
Wet Lab:
Education and public engagement:
Wet lab:
Wet lab:
Wet lab:
Human Practices:
Education and Public Involvement:
Wet lab:
Wet Lab:
Integrated Human practices/Entrepreneurship:
I toss and turn, I keep stressing my mind, mind (wiki, wiki)
I look for peace but see I don't attain (wiki, wiki)
What I need for keeps this silly game we play, play
Now look at this (wiki, wiki)
Madness to magnet keeps attracting me, me (wiki, wiki)
I try to run but see I'm not that fast (wiki, wiki)
I think I'm first but surely finish last, last