Difference between revisions of "Team:UiOslo Norway/Safety"

 
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<h1 class="h1-font-other"> Safety </h1>
 
<h1 class="h1-font-other"> Safety </h1>
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<!-- Old content from iGEM
 
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
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<h1> Add our project information below this</h1>
 
  
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<p> White List </p>
 
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<h6> Antibiotic Resistant Factor </h6>
 
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<p> White List </p>
 
  
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<h1> Add our project information above this</h1>
 
  
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<h5>Safe Project Design</h5>
 
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<div id="tab-container">
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                <div class="row">
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<div class="col-md-12">
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                        <ul id="tab2" class="nav nav-pills">
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                            <li class="active"><a href="#tab-item1" data-toggle="tab">Laboratories</a></li>
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                            <li><a href="#tab-item2" data-toggle="tab">Microorganisms used</a></li>
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                            <li><a href="#tab-item3" data-toggle="tab">GMOs and Antimicrobial resistance management</a></li>
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                            <li><a href="#tab-item4" data-toggle="tab">Biosafety Training</a></li>
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                        </ul>
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                        <div class="tab-content">
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                            <div class="tab-pane fade active in" id="tab-item1">
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<br> <br>
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A biosafety level (BSL) is a collection of biocontainment measures required to isolate dangerous biological materials in an enclosed laboratory facility. The grades of containment range from the lowest biosafety level 0 (BSL-0) to the highest at level 4 (BSL-4).
 +
<p>Source: Richmond JY, McKinney RW (editors) (1999). Biosafety in Microbiological and Biomedical Laboratories<p>
 +
    <h3>1.</h3>
 +
              <pre><b> Laboratory      Safety Level  </b><br> Dirk's Lab    BSL2 (moderate risk)      </pre>       
 +
<b> What has it been done in this lab: </b>
 +
 +
Transformation of competent cells, plating in agar plates with antibiotics, inoculation of bacteria in liquid medium, incubations and  all the steps of purification of sfgfp from transformed bacteria and finally the termination of Genetically modified organisms (GMOs) waste by chlorine. (in accordance to HSE safety rules/regulations of the University of Oslo (UiO)).
 +
<a href="https://www.sikresiden.no/en/preventive/labs_workshops_clinics">More Info</a>.<br>
 +
<img class="safety-picture" src="https://static.igem.org/mediawiki/2017/1/1f/T--UiOslo_Norway--dirkLab1.jpg">
 +
<br> <br>
 +
 +
<h3>2.</h3>
 +
<pre><b> Laboratory      Safety Level  </b><br> CEES Lab    BSL1      </pre> 
 +
 +
<b> What has it been done in this lab: </b>
 +
 +
Cloning, PCR, Electrophoresis  (in accordance to HSE safety precautions of the University of Oslo:
 +
<a href="https://www.sikresiden.no/en/preventive/labs_workshops_clinics">More Info</a>.<br>
 +
<img class="safety-picture" src="https://static.igem.org/mediawiki/2017/6/68/T--UiOslo_Norway--ceesLab.jpg">
 +
<br> <br>
 +
<h3>3.</h3>
 +
<pre><b> Laboratory      Safety Level  </b><br> Lopez-Aviles Laboratory    BSL1      </pre>
 +
 +
<b> What has it been done in this lab: </b>
 +
 +
Transformation of Schizosaccharomyces pombe, electrophoresis and restriction digestion studies and Yeast cell culture. in accordance to HSE safety rules of the University of Oslo.
 +
<a href="https://www.sikresiden.no/en/preventive/labs_workshops_clinics">More Info</a>.<br>
 +
<img class="safety-picture"  src="https://static.igem.org/mediawiki/2017/3/30/T--UiOslo_Norway--Lopez-AvilesLaboratory2.jpg">
 +
<img class="safety-picture"  src="https://static.igem.org/mediawiki/2017/8/84/T--UiOslo_Norway--Lopez-AvilesLaboratory1.jpg">
 +
 +
 +
</div>
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                            <div class="tab-pane fade" id="tab-item2">
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<table class="customers">
 +
<caption>Table: Organisms Used: </caption>
 +
  <tr>
 +
    <th>Organism name  (used in this project) </th>
 +
    <th>Risk Group</th>
 +
  </tr>
 +
  <tr>
 +
    <td>Schizosaccharomyces pombe  (972h-)<br><img class="safety-picture" src="https://static.igem.org/mediawiki/2017/7/7e/T--UiOslo_Norway--Fission_yeast.jpg"><br>
 +
Microscopic view of a fission yeast culture.
 +
<p>Source: The Cell Cycle. Principles of Control. Author David O Morgan</p>
 +
 +
</td>
 +
    <td>G1</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Saccharomyces cerevisiae <br><img class="safety-picture"  src="https://static.igem.org/mediawiki/2017/1/16/T--UiOslo_Norway--Saccharomyces_cerevisiae_SEM.jpg"><br>
 +
Saccharomyces cerevisiae, SEM image
 +
<p>Source: Sample Preparations for Scanning Electron Microscopy – <br>Life Sciences  Author Mogana Das Murtey and Patchamuthu Ramasamy
 +
</p>
 +
</td>
 +
    <td>G1</td>
 +
  </tr>
 +
  <tr>
 +
    <td>E-coli strains (e.g. DH5alpha, TOP10, BL21 Gold) <br> <img class="safety-picture"  src="https://static.igem.org/mediawiki/2017/3/33/T--UiOslo_Norway--E._coli_colony.jpg"> <br>
 +
SEM micrograph artificially colored.
 +
<p>Source: Phil Moyer. Photo courtesy CDC/Janice Haney Carr. <br>Author Photo courtesy CDC/Janice Haney Carr.</p>
 +
</td>
 +
    <td>G1</td>
 +
  </tr>
 +
 
 +
</table>
 +
 +
 +
 +
 +
 +
 +
                            </div>
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                            <div class="tab-pane fade" id="tab-item3">
 +
                               
 +
<img class="safety-picture"  src="https://static.igem.org/mediawiki/2017/9/92/T--UiOslo_Norway--GMOs.jpg">
 +
<p>Source: http://www.emro.who.int/health-topics/antimicrobial-resistance/index.html </p>
 +
 +
<br><br>
 +
A <b>genetically modified organism </b>(GMO) is an organism whose genetic material has been modified using genetic engineering methods (i.e., a genetically modified organism). GMOs are used nowadays to produce variety of medications and also a more challenging area; genetically modified foods (GMF). It is also used in scientific research and production of other goods.
 +
<p>Source: History of Genetically Modified Foods". umich.edu. 21 October 2015</p>
 +
 +
<b>Antimicrobial resistance</b> (AMR) is the ability of a microorganism (like bacteria, viruses, and also parasites) to neutralize an antimicrobial (e.g. antibiotics and antivirals) from working properly against it. As a outcome, standard treatments get ineffective, infections persist and could potentially spread to others.
 +
<p><a href="http://www.who.int/antimicrobial-resistance/en/">Source: http://www.who.int/antimicrobial-resistance/en/</a></p>
 +
 +
<b>World Health Organization</b> has provided a List of critical important Antibiotics to  address this issue.<br><br>
 +
 +
<a href="http://www.who.int/foodsafety/areas_work/antimicrobial-resistance/cia/en/">See WHO list of Critically Important Antimicrobials (CIA*)</a>
 +
<img class="safety-picture"  src="https://static.igem.org/mediawiki/2017/3/35/T--UiOslo_Norway--cia5.JPG">
 +
<p>source: http://www.who.int/foodsafety/areas_work/antimicrobial-resistance/cia/en/</p>
 +
 +
<b>Department of Bioscience</b>, UiO has provided a set of rules/regulations to ensure working safely in lab. In particular, Local HSE criteria of  Department of Bioscience also ensures to protect environment  from spreading of AMR and GMOs from Lab facilities to surrounding nature.
 +
For this end, these procedures must be fulfilled before, during and at the end of Lab work:
 
<ul>
 
<ul>
−
<li>Choosing a non-pathogenic chassis</li>
+
<li>Procedure for work with Biological substances  </li>
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
<li>Procedure for working with genetically modified organisms (GMO)</li>
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
<li>Procedure for waste management, including laboratory waste</li>
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
<li>Local procedure for health and safety training and access to laboratories</li>
 +
<li>Local procedure for health and safety training and access to laboratories</li>
 
</ul>
 
</ul>
  
  
 +
For detailed information, see
 +
<a href="http://www.mn.uio.no/ibv/english/about/hss/prosedyrer/procedure-laboratorywaste-1.html"> Procedure-laboratorywaste(UiO)</a>
 +
<a href="http://www.mn.uio.no/ibv/english/about/hss/prosedyrer/"> HSE </a>  <br>
  
 +
<b> Library of common procedures: </b> <br><br>
  
−
<h5>Safe Lab Work</h5>
+
In the case of lack of a defined procedure, the library of common procedure, University of Oslo, gives the way to submit a new SOP; this happens after a comprehensive risk assessment analysis by safety specialists and confirmation by Safety Dean of Deaprtment.
 +
<a href="https://www.mn.uio.no/ibv/english/about/hss/sop/"> See More info </a> <br>
 +
 
 +
<b>Resistance factors used in this project: </b>
 +
<table>
 +
  <tr>
 +
    <th><b>Name </b></th>
 +
    <th><b>Safety precautions </b></th>
 +
  </tr>
 +
  <tr>
 +
    <td>Ampicillin </td>
 +
    <td>not included in the critical important CIA list</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Chloramphenicol </td>
 +
    <td>not included in the critical important CIA list</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Kanamycin </td>
 +
    <td>not included in the critical important CIA list</td>
 +
  </tr>
 +
</table>
 +
 
 +
<b>Safety measures undertaken by team:  </b> <br><br>
 +
 
 +
<ul>
 +
<li>Training for all the team by supervisors before lab work</li>
 +
<li>Working in Biosafety BSL2 Lab (in the time of working with Bacteria)</li>
 +
<li>Safe Discarding of Gloves, small samples etc. in accordance to Local HSE procedures </li>
 +
<li>Chemically termination of the GMO containing materials before discarding in accordance to  Local HSE procedures</li>
 +
</ul>
 +
 
 +
<image class="safety-picture" src="https://static.igem.org/mediawiki/2017/a/a1/T--UiOslo_Norway--Disposal1.jpeg">
 +
<image class="safety-picture"  src="https://static.igem.org/mediawiki/2017/d/d3/T--UiOslo_Norway--Disposal2.jpeg">
 +
<image class="safety-picture"  src="https://static.igem.org/mediawiki/2017/4/43/T--UiOslo_Norway--Disposal3.jpeg">
 +
 
 +
Disposal of large hazardous waste containers
 +
<p>Source: University of Oslo official website</p>
 +
 
 +
 
 +
 
 +
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
                            </div>
 +
<div class="tab-pane fade" id="tab-item4">
 +
<br><br>
 +
Lab hazards can bear risk to your health and GMOs can potentially affect environmental health/balance.
 +
It is essential that lab workers to be aware regarding safety precautions and safe removal before/during/after lab work. All students at the UiO should receive HSE training specialized to the faculty they belong to. These are mandatory courses starting up during the first weeks of every semester. This kind of training ensures that all students, as well as other members of the working environment, have the necessary training to perform for their duties and fulfill their responsibilities put forward by their work.
 +
<a href="http://www.uio.no/studier/emner/matnat/kjemi/HMS0503/index-eng.html"> More info </a>
 +
 
 +
Moreover, a customized training also was set up for the whole team before initiation of project at BSL2 and BSL1 labs respectively, conducted by Lab Managers: Professor Dirk Linke, Professor Sandra Lopez Avilez and Ninna... Anders Moien assisted us as the safety advisor from the department of Bioscience in this project. This customized training by supervisors covered these headlines: <br><br>
 +
 
 +
<ul>
 +
<li>appropriate use of personal protective equipment (PPE) </li>
 +
<li>proper waste disposal </li>
 +
<li>and risk assessment </li>
 +
Also, two mandatory act  was determined by supervisors in the time of working at BSL2:
 +
</ul>
 +
 
 +
<ul>
 +
<li>Laboratory work: Minimum two people, Maximum four </li>
 +
<li>Laboratory work: Working under supervision of at least one instructor all the times</li>
 +
</ul>
 +
 
 +
<h3>Research ethics</h3>
 +
 
 +
University of Oslo (UiO)  keeps a strong focus on research ethics. For this end,  It  has prepared a plan of action for preventing academic dishonesty and providing a positive environment.
 +
 
 +
A researcher have a responsibility to comply his/her work according to:
 +
 
 +
standards of good research practice
 +
established scientific and ethical principles
 +
In this way UiO aims to  research ethics and research safety together. Oslo iGEM team 2017 was committed to proceed their project in accordance with the mentioned criteria and official ethical guidelines approved by University of Oslo.
 +
 
 +
For detailed information about this guideline please <a href="http://www.uio.no/english/for-employees/support/research/ethics/index.html">visit </a>
 +
 
 +
 
 +
                            </div>
 +
 
 +
 
 +
 
 +
 
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
            </div><!--/#table-container-->
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
  
  
−
<h5>Safe Shipment</h5>
 
  
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p> 
 
  
−
Our content goes below-->
 
  
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<pre><b> Laboratory      Safety Level  <b> <br> </pre>
 
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<pre>    Dirk's Lab    L2 (moderate risk)      </pre>
 
  
  
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{{UiOslo_Norway/footerTemplate}}

Latest revision as of 02:38, 2 November 2017


Safety



A biosafety level (BSL) is a collection of biocontainment measures required to isolate dangerous biological materials in an enclosed laboratory facility. The grades of containment range from the lowest biosafety level 0 (BSL-0) to the highest at level 4 (BSL-4).

Source: Richmond JY, McKinney RW (editors) (1999). Biosafety in Microbiological and Biomedical Laboratories

1.

 Laboratory      Safety Level  
 Dirk's Lab     BSL2 (moderate risk)
What has it been done in this lab: Transformation of competent cells, plating in agar plates with antibiotics, inoculation of bacteria in liquid medium, incubations and all the steps of purification of sfgfp from transformed bacteria and finally the termination of Genetically modified organisms (GMOs) waste by chlorine. (in accordance to HSE safety rules/regulations of the University of Oslo (UiO)). More Info.


2.

 Laboratory      Safety Level  
 CEES Lab     BSL1
What has it been done in this lab: Cloning, PCR, Electrophoresis (in accordance to HSE safety precautions of the University of Oslo: More Info.


3.

 Laboratory      Safety Level  
 Lopez-Aviles Laboratory     BSL1
What has it been done in this lab: Transformation of Schizosaccharomyces pombe, electrophoresis and restriction digestion studies and Yeast cell culture. in accordance to HSE safety rules of the University of Oslo. More Info.
Table: Organisms Used:
Organism name (used in this project) Risk Group
Schizosaccharomyces pombe (972h-)

Microscopic view of a fission yeast culture.

Source: The Cell Cycle. Principles of Control. Author David O Morgan

 G1
Saccharomyces cerevisiae

Saccharomyces cerevisiae, SEM image

Source: Sample Preparations for Scanning Electron Microscopy –
Life Sciences Author Mogana Das Murtey and Patchamuthu Ramasamy

G1
E-coli strains (e.g. DH5alpha, TOP10, BL21 Gold)

SEM micrograph artificially colored.

Source: Phil Moyer. Photo courtesy CDC/Janice Haney Carr.
Author Photo courtesy CDC/Janice Haney Carr.

 G1

Source: http://www.emro.who.int/health-topics/antimicrobial-resistance/index.html



A genetically modified organism (GMO) is an organism whose genetic material has been modified using genetic engineering methods (i.e., a genetically modified organism). GMOs are used nowadays to produce variety of medications and also a more challenging area; genetically modified foods (GMF). It is also used in scientific research and production of other goods.

Source: History of Genetically Modified Foods". umich.edu. 21 October 2015

Antimicrobial resistance (AMR) is the ability of a microorganism (like bacteria, viruses, and also parasites) to neutralize an antimicrobial (e.g. antibiotics and antivirals) from working properly against it. As a outcome, standard treatments get ineffective, infections persist and could potentially spread to others.

Source: http://www.who.int/antimicrobial-resistance/en/

World Health Organization has provided a List of critical important Antibiotics to address this issue.

See WHO list of Critically Important Antimicrobials (CIA*)

source: http://www.who.int/foodsafety/areas_work/antimicrobial-resistance/cia/en/

Department of Bioscience, UiO has provided a set of rules/regulations to ensure working safely in lab. In particular, Local HSE criteria of Department of Bioscience also ensures to protect environment from spreading of AMR and GMOs from Lab facilities to surrounding nature. For this end, these procedures must be fulfilled before, during and at the end of Lab work:
  • Procedure for work with Biological substances
  • Procedure for working with genetically modified organisms (GMO)
  • Procedure for waste management, including laboratory waste
  • Local procedure for health and safety training and access to laboratories
  • Local procedure for health and safety training and access to laboratories
For detailed information, see Procedure-laboratorywaste(UiO) HSE
Library of common procedures:

In the case of lack of a defined procedure, the library of common procedure, University of Oslo, gives the way to submit a new SOP; this happens after a comprehensive risk assessment analysis by safety specialists and confirmation by Safety Dean of Deaprtment. See More info
Resistance factors used in this project:
Name Safety precautions
Ampicillin not included in the critical important CIA list
Chloramphenicol not included in the critical important CIA list
Kanamycin not included in the critical important CIA list
Safety measures undertaken by team:

  • Training for all the team by supervisors before lab work
  • Working in Biosafety BSL2 Lab (in the time of working with Bacteria)
  • Safe Discarding of Gloves, small samples etc. in accordance to Local HSE procedures
  • Chemically termination of the GMO containing materials before discarding in accordance to Local HSE procedures
Disposal of large hazardous waste containers

Source: University of Oslo official website



Lab hazards can bear risk to your health and GMOs can potentially affect environmental health/balance. It is essential that lab workers to be aware regarding safety precautions and safe removal before/during/after lab work. All students at the UiO should receive HSE training specialized to the faculty they belong to. These are mandatory courses starting up during the first weeks of every semester. This kind of training ensures that all students, as well as other members of the working environment, have the necessary training to perform for their duties and fulfill their responsibilities put forward by their work. More info Moreover, a customized training also was set up for the whole team before initiation of project at BSL2 and BSL1 labs respectively, conducted by Lab Managers: Professor Dirk Linke, Professor Sandra Lopez Avilez and Ninna... Anders Moien assisted us as the safety advisor from the department of Bioscience in this project. This customized training by supervisors covered these headlines:

  • appropriate use of personal protective equipment (PPE)
  • proper waste disposal
  • and risk assessment
    • Also, two mandatory act was determined by supervisors in the time of working at BSL2:
  • Laboratory work: Minimum two people, Maximum four
  • Laboratory work: Working under supervision of at least one instructor all the times

Research ethics

University of Oslo (UiO) keeps a strong focus on research ethics. For this end, It has prepared a plan of action for preventing academic dishonesty and providing a positive environment. A researcher have a responsibility to comply his/her work according to: standards of good research practice established scientific and ethical principles In this way UiO aims to research ethics and research safety together. Oslo iGEM team 2017 was committed to proceed their project in accordance with the mentioned criteria and official ethical guidelines approved by University of Oslo. For detailed information about this guideline please visit

Problemveien 7, Oslo, Norway

uioslonorway@gmail.com