Difference between revisions of "Team:CCA San Diego/Measurement"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h2 style="font-size:79px">Measurement</h2>
  
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<h6>CCA_San_Diego created a method for measuring the effective nature of PAH degradation by substituting the media’s standard carbon source for PAH compounds, therefore rendering the bacteria without an energy supply unless the pathway of PAH degradation succeeds and efficiently leads to the creation of two substrates: salicylate and phthalate (which supply carbon for the Krebs Cycle in E. coli). By doing this, growth curves can be effectively used to monitor the degradation efficiency and progression over time as bacterial colonies grow.</h6>
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<h1>Measurement</h1>
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<p>There are a lot of exciting parts in the Registry, but many parts have still not been characterized. Synthetic Biology needs great measurement approaches for characterizing new parts, and efficient new methods for characterizing many parts at once. If you've done something exciting in the area of Measurement, describe it here!</p>
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<h3>Best Innovation in Measurement Special Prize</h3>
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<p>If you've done excellent work in measurement, you should consider nominating your team for this special prize. Designing great measurement approaches for characterizing new parts or developing and implementing an efficient new method for characterizing thousands of parts are good examples.
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To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Innovation in Measurement prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>.
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You must also delete the message box on the top of this page to be eligible for this prize.
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<br><h6>Figure 1. Time course biotransformation experiments using crude oil samples from Pennsylvania (PA), Saudi Arabia, and Ecuador, measuring absorbance at 600nm of E.coli BL21 recombinant cultures containing the fluorene and phenanthrene catabolic pathways or control vectors. MM=M9 minimal medium.</h6>
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<h6>Results show growth in oil or isolated PAH is equivalent to easy to access carbon source (Glucose).</h6>
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<h6>Carbon is the building block of organic molecules and makes up a large portion of the structures in life forms. Thus to grow and reproduce, a method of breaking down and accessing carbon is necessary. In this context, using a singular carbon source in a growth setup for bacteria allows us to isolate if the bacteria degrade and process complex hydrocarbons to form molecules capable of facilitating life and growth. This measurement technique is very useful in testing bioremediation projects that involve degradation</h6>
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<h6>In our tests, Fluorene or Phenanthrene was used a singular carbon source. Since bacteria need some source of carbon to grow, they must be using the only carbon source present when growing. The growth curves indicate that the bacteria with the pathways inserted are growing at rates comparable with a common carbon source, Glucose. This means the bacteria are degrading the PAHs and consuming the substrate products.</h6>
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<h6>Our methods are innovative, as they utilize normally harmful PAHs as sources of carbon to allow bacteria to undergo cellular respiration. In an ironic twist of fate, we used the growth of one organism to measure another compound’s degradation. Our methods are logical and apply basic processes of science to better the world around us. </h6>
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<h5>Inspiration</h5>
 
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<p>You can look at what other teams did to get some inspiration! <br />
 
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Here are a few examples:</p>
 
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<li><a href="https://2016.igem.org/Team:Stanford-Brown">2016 Stanford-Brown</a></li>
 
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<li><a href="https://2016.igem.org/Team:Genspace">2016 Genspace</a></li>
 
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<li><a href="https://2015.igem.org/Team:William_and_Mary">2015 William and Mary</a></li>
 
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<li><a href="https://2014.igem.org/Team:Aachen">2014 Aachen  </a></li>
 
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Latest revision as of 03:42, 2 November 2017

Measurement

CCA_San_Diego created a method for measuring the effective nature of PAH degradation by substituting the media’s standard carbon source for PAH compounds, therefore rendering the bacteria without an energy supply unless the pathway of PAH degradation succeeds and efficiently leads to the creation of two substrates: salicylate and phthalate (which supply carbon for the Krebs Cycle in E. coli). By doing this, growth curves can be effectively used to monitor the degradation efficiency and progression over time as bacterial colonies grow.


Figure 1. Time course biotransformation experiments using crude oil samples from Pennsylvania (PA), Saudi Arabia, and Ecuador, measuring absorbance at 600nm of E.coli BL21 recombinant cultures containing the fluorene and phenanthrene catabolic pathways or control vectors. MM=M9 minimal medium.

Results show growth in oil or isolated PAH is equivalent to easy to access carbon source (Glucose).

Carbon is the building block of organic molecules and makes up a large portion of the structures in life forms. Thus to grow and reproduce, a method of breaking down and accessing carbon is necessary. In this context, using a singular carbon source in a growth setup for bacteria allows us to isolate if the bacteria degrade and process complex hydrocarbons to form molecules capable of facilitating life and growth. This measurement technique is very useful in testing bioremediation projects that involve degradation

In our tests, Fluorene or Phenanthrene was used a singular carbon source. Since bacteria need some source of carbon to grow, they must be using the only carbon source present when growing. The growth curves indicate that the bacteria with the pathways inserted are growing at rates comparable with a common carbon source, Glucose. This means the bacteria are degrading the PAHs and consuming the substrate products.

Our methods are innovative, as they utilize normally harmful PAHs as sources of carbon to allow bacteria to undergo cellular respiration. In an ironic twist of fate, we used the growth of one organism to measure another compound’s degradation. Our methods are logical and apply basic processes of science to better the world around us.

email igemcca@gmail.com
Canyon Crest Academy iGEM 2017 CC;