Difference between revisions of "Team:ITB Indonesia/Attributions"

 
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<div style="background: #e8e6d1; padding: 30px; color: #1c2922">
 
<div style="background: #e8e6d1; padding: 30px; color: #1c2922">
 
<h1 class="ITB_h1" style="padding-bottom: 60px; margin-bottom: 10px; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; font-size: 100px; text-align: center; color: #1c2922">WORKS!</h1>
 
<h1 class="ITB_h1" style="padding-bottom: 60px; margin-bottom: 10px; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; font-size: 100px; text-align: center; color: #1c2922">WORKS!</h1>
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<p style="font-style: ITB_h1;text-align: justify; color: #1c2922; margin-bottom: 50px;">All the basic molecular techniques in the lab is performed by the students in the team. We designed the synthetic gBlocks, cloned them into vector, and performed the characterization of respective BioBricks. </p>
  
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<p style="font-style: ITB_h1;text-align: justify; color: #1c2922; margin-bottom: 50px;">All the basic molecular techniques in the lab is performed by the students in the team. We designed the synthetic gBlocks, cloning them into vector, </p>
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<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Project Advisors</h1>
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<p style="font-style: ITB_h1; font-size: 50px; text-align: center; color: #1c2922; "><b>Dr. Maelita R. Moeis</b></p><center>
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<table class="table" style="background: #e8e6d1">
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<img src="https://static.igem.org/mediawiki/2017/6/68/ITB_INDONESIA_IGEM2015_ibu_mae.jpg
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" style="width: 50%; height: auto;"/></center>
  
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<p style="font-style: ITB_h1; font-size: 50px; text-align: center; color: #1c2922; margin-bottom: -30px ">Project Idea</p>  
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<p style="font-style: ITB_h1; font-size: 50px; text-align: center; color: #1c2922; "><b>Dr. Sony Suhandono</b></p>
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<center>
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<img src="https://static.igem.org/mediawiki/2017/5/5c/ITB_INDONESIA_IGEM2015_pak_sony.jpg
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" style="width: 50%; height: auto;"/></center>
  
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<p>The idea is based on the previous (a href="https://2014.igem.org/Team:ITB_Indonesia"> ITB iGEM 2014 </a> project which is called ColiPlaster. The 2017 team developed the idea for the optimization and novel enzymes. <br>
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<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Project Idea</h1>
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<p>The idea is based on the previous <a href="https://2014.igem.org/Team:ITB_Indonesia" style="color:black"> ITB iGEM 2014 </a> project which is called ColiPlaster. The 2017 team developed the idea for the optimization and novel enzymes. <br>
 
We also get many inspirations from:<br>
 
We also get many inspirations from:<br>
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• <a href="https://2012.igem.org/Team:University_College_London"> Plastic Republic </a> , <br>
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• <a href="https://2012.igem.org/Team:University_College_London" style="color:black" > Plastic Republic </a> , <br>
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• <a href="https://2012.igem.org/Team:UC_Davis">Team UC Davis , and <br>
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• <a href="https://2012.igem.org/Team:UC_Davis" style="color:black">Team UC Davis</a>, and <br>
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• <a href="ttp://2016.igem.org/Team:BGU_ISRAEL">PlastiCure <br>
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• <a href="ttp://2016.igem.org/Team:BGU_ISRAEL" style="color:black">PlastiCure </a> <br>
 
  </p>
 
  </p>
  
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<p style="font-style: ITB_h1;text-align: justify; color: #1c2922; margin-bottom: 50px;">We need to test our idea by interviewing stakeholders in micro-plastic research. We wanted to primarily know how micro-plastic forms in the perspective of Oceanography, followed by identifications of the most affected sectors.  Dr. Ivonne Milichristi Radjawane has conducted researches on plastic debris in Pelabuhan Ratu Bay, Jakarta Bay, Bali Bay and Ambon Bay. She said, “There are two factors causing the plastic to accumulate in the sea, wave and current”. She also gave an insight into our project prospect, “75% of garbage thrown into the sea are plastic-based, Indonesia has been recently highlighted internationally as the second worst plastic polluters to the sea.”. According to her, the Indonesian government has been massively intensifying the work to reduce plastic pollution since then. “The government has a target to reduce plastic pollution up to 70% by the year 2020”, she said. She also suggests our team to conduct a survey on Jakarta Bay to see how the micro-plastics affect the locals there. “The research conducted in Makassar Strait proved micro-plastics inflict dreadful effects to marine lives, shown by guts of fish filled with micro-plastic fragments” she implied. Overall, she teaches us a valuable lesson for our project. Her perspective inspires us to develop an on-site application for our project.</p>
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<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Project References</h1>
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<p>The main enzyme used in this project <a href="http://www.uniprot.org/uniprot/A0A0K8P6T7" style="color:#1c2922"> PETase </a> is based on the research paper: <br>
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<b>Yoshida S., Hiraga K., Takehana T., Taniguchi I., Yamaji H., Maeda Y., Toyohara K., Miyamoto K., Kimura Y., Oda K.</b> 2016. A bacterium that degrades and assimilates poly (ethylene terephthalate). <i>Science</i> 351:1196-1199</p>
  
  
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<p style="font-style: ITB_h1; font-size: 50px; text-align: center; color: #1c2922; margin-bottom: -30px ">Cidaun and Muara Angke </p>
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<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Wetlab Support</h1>
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<p style="font-style: ITB_h1; font-size: 28px; text-align: center; color: #1c2922; ">Fisherman Interview</p>
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<p>
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<b>Azzania FIbriani, Ph. D</b> as Head of Molecular and Chromosome Analysis Lab, School of Life Science and Technology, Institut Teknologi Bandung, who provided us lab spaces and permission to use the instruments inside the lab.<br><br>
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<b>Dr. Tati Kristianti, M. Sc.,</b> as Research Assistant from Genetics and Molecular Biotechnology group SITH ITB, who assisted us in providing the kit, enzymes, and helped us in anything related to customs and online ordering.<br><br>
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<b>Ika Agus Rini, M. Sc.,</b> our team mentor, who provide insights and experience on iGEM.<br><br>
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<b>Rahmat Azhari Kemal, M. Sc.,</b> last year Wetlab coordinator, who has given us insights on first steps in starting the lab work.<br><br>
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<b>Agus Eka Prasetyo, S. Si,</b> who first gives us the idea and tutor on using Restriction-Free Cloning techniques in ligating our constructs.<br><br>
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</p>
  
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<p style="font-style: ITB_h1; font-size: 50px; text-align: center; color: #1c2922; margin-bottom: -30px ">Biological Safety Expert Talks</p>
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<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Modelling Support</h1>
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<p style="font-style: ITB_h1; font-size: 28px; text-align: center; color: #1c2922;"><a href="https://sith.itb.ac.id/sriharjati/"style="color:#1c2922">Ir. Sri Harjati Suhardi, Ph.D</a> </p>
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<p><b>Mochamad Apri, Ph. D.,</b> lecturer from Mathematics Study Program ITB, who has given the modelling team insightful analysis on constructing the model.</p>
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<center><img src="https://static.igem.org/mediawiki/2017/1/18/T--ITB_Indonesia--Bu_Reni.png
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" style="width: 300px; height: auto; img-align:center"></center>
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<p style="font-style: ITB_h1; font-size: 50px; text-align: center; color: #1c2922; ">Indonesia iGEM Booklet Vol. 1</p>
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<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Human Practice Support</h1>
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<p><b>Sri Harjati Suhardi, Ph. D,</b> lecturer from Microbial Biotechnology group, who has given us experienced insights on bioremediation, especially in Indonesia.</p>
  
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<p style="font-style: ITB_h1;text-align: justify; color: #1c2922; margin-bottom: 50px;">Public engagement program gave us an overview about people awareness on synthetic biology. We are trying to increase the awareness by writing a booklet. The booklet was written in Bahasa to prevent a language barrier in the learning process. It is consist of the synthetic biology basic knowledge and our projects. The basic knowledge includes the definition of synthetic biology, part, device, and system. We also elaborate the previous, recent and future outlook of our iGEM projects. We put the past four years of ITB team human practice, dry lab, and wet lab in detail. It was our contribution to inspire another Indonesian team. The booklet then distributed to academician through an email. </p>
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<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Our Sponsors
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<p style="font-style: ITB_h1; font-size: 50px; text-align: center; color: #1c2922; margin-bottom: -30px">Short Documenter</p>
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<center><img src="https://static.igem.org/mediawiki/2017/9/9d/T--ITB_Indonesia--AT1.png" style="width: 700px; height: 400; img-align:center"</center>
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<p style="font-style: ITB_h1; font-size: 28px; text-align: center; color: #1c2922; ">Dewaruci</p>
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</h1>

Latest revision as of 03:43, 2 November 2017

Team Attributions
Collaborations Overview Design Notebook Contribution Results Improve
Basic Parts Composite Part Part Collection
Silver HP Integrated and Gold Public Engagement


Attributions


Acknowledgement

This project wouldn't be possible without commitment and dedication from many parties. It is our greatest pleasure to acknowledge the assistance and contribution of the individuals and groups who have helped us towards the successful completion of the project.

WORKS!

All the basic molecular techniques in the lab is performed by the students in the team. We designed the synthetic gBlocks, cloned them into vector, and performed the characterization of respective BioBricks.

Project Advisors

Dr. Maelita R. Moeis

Dr. Sony Suhandono

Project Idea

The idea is based on the previous ITB iGEM 2014 project which is called ColiPlaster. The 2017 team developed the idea for the optimization and novel enzymes.
We also get many inspirations from:
• Plastic Republic ,
• Team UC Davis, and
• PlastiCure

Project References

The main enzyme used in this project PETase is based on the research paper:
Yoshida S., Hiraga K., Takehana T., Taniguchi I., Yamaji H., Maeda Y., Toyohara K., Miyamoto K., Kimura Y., Oda K. 2016. A bacterium that degrades and assimilates poly (ethylene terephthalate). Science 351:1196-1199

Wetlab Support

Azzania FIbriani, Ph. D as Head of Molecular and Chromosome Analysis Lab, School of Life Science and Technology, Institut Teknologi Bandung, who provided us lab spaces and permission to use the instruments inside the lab.

Dr. Tati Kristianti, M. Sc., as Research Assistant from Genetics and Molecular Biotechnology group SITH ITB, who assisted us in providing the kit, enzymes, and helped us in anything related to customs and online ordering.

Ika Agus Rini, M. Sc., our team mentor, who provide insights and experience on iGEM.

Rahmat Azhari Kemal, M. Sc., last year Wetlab coordinator, who has given us insights on first steps in starting the lab work.

Agus Eka Prasetyo, S. Si, who first gives us the idea and tutor on using Restriction-Free Cloning techniques in ligating our constructs.

Modelling Support

Mochamad Apri, Ph. D., lecturer from Mathematics Study Program ITB, who has given the modelling team insightful analysis on constructing the model.

Human Practice Support

Sri Harjati Suhardi, Ph. D, lecturer from Microbial Biotechnology group, who has given us experienced insights on bioremediation, especially in Indonesia.

Our Sponsors