Difference between revisions of "Team:Amsterdam"

 
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    <nav class="navbar navbar-inverse">
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    <div class="container-fluid">
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      <div class="navbar-header">
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      <img src="https://static.igem.org/mediawiki/2017/8/86/TAmsterdam_pm_logo.png"/>
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      <a class="navbar-brand nav-link" href="https://2017.igem.org/Team:Amsterdam">
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        Photosynthetic Magic
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      </a>
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      </div>
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      <ul class="list-inline nav navbar-right">
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      <li class="list-inline-item dropdown">
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        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Project">
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        Project
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        <span class="caret">
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        </span>
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        </a>
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        <ul class="dropdown-menu">
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        <li>
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Project">
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          Overview
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        <li>
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Produce">
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          Production
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        <li>
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Export">
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          Exportation
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        <li>
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Detect">
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          Detection
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        </li>
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        </ul>
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      <li class="list-inline-item">
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        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Model">
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        Model
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        </a>
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      <li class="list-inline-item dropdown">
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        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Practices">
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        Practices
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        <span class="caret">
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Practices">
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          Overview
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          </a>
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        <li>
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Carbon">
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          Carbon Efficiency
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          </a>
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        <li>
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/HP/Silver">
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          HP Silver
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          </a>
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        <li>
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/HP/Gold_Integrated">
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          HP Gold
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          </a>
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        <li>
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Engagement">
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          Engagement
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          </a>
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        <li>
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Safety">
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          Safety
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        </ul>
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        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Lab">
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        Lab
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Lab">
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          Overview
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Parts">
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          Parts
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/InterLab">
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          Interlab Study
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/LabJournal">
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          Lab Journal
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        <li>
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Protocol">
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          Protocols
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Methods">
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          Methods
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        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Team">
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        Team
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        <span class="caret">
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        <ul class="dropdown-menu">
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Team">
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          Overview
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Attributions">
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          Attributions
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Collaborations">
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          Collaborations
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          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Sponsors">
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          Sponsors
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        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Achievements">
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        Achievements
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    <div class="inner cover">
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      <video controls="" height="auto" poster="https://static.igem.org/mediawiki/2017/8/8f/Intro_poster.png" width="85%">
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      <source src="https://static.igem.org/mediawiki/2017/4/4c/TAmsterdam_intro.mp4" type="video/mp4"/>
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      <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Project" id="Project">
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      <img alt="Project" src="https://static.igem.org/mediawiki/2017/9/9b/TAmsterdam_project.png">
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        Project
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      <p>
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        Practices
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      <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Lab" id="Lab">
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      <img alt="Lab" src="https://static.igem.org/mediawiki/2017/f/f3/TAmsterdam_lab.png"/>
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      <p>
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        Lab
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      <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Team" id="Team">
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      <img alt="Team" src="https://static.igem.org/mediawiki/2017/0/06/TAmsterdam_team.png"/>
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      <p>
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        Team
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      </p>
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      <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Achievements" id="Achievements">
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      <img alt="Achievements" src="https://static.igem.org/mediawiki/2017/9/94/TAmsterdam_achievements.png"/>
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      <p>
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        Achievements
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        <div class="home-intro-container" id="first">
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        <img src="https://static.igem.org/mediawiki/2017/6/66/TAmsterdam_practices.png"/>
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        <p>
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          We are running out of oil and the climate is changing drastically due to the emission of greenhouse gases such as CO
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          <sub>
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          2
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          </sub>
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          .
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        <img src="https://static.igem.org/mediawiki/2017/e/ed/TAmsterdam_recycle.png"/>
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        <p>
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          But what if CO
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          <sub>
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          2
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          </sub>
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          were a resource, rather than a wasteful pollutant; and might even replace oil?
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        </p>
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      <div class="home-content-container">
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        <div class="home-intro-container" id="first">
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        <img src="https://static.igem.org/mediawiki/2017/8/8a/TAmsterdam_plants.png"/>
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        <p>
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          Early attempts to achieve this have focused on producing sugars with plants, which are then subsequently fermented by bacteria to produce liquid fuels and other chemicals.
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        </p>
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        </div>
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      </div>
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      <div class="home-content-container">
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        <div class="home-intro-container" id="second">
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        <img src="https://static.igem.org/mediawiki/2017/9/9b/TAmsterdam_project.png"/>
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        <p>
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          But there is a better way, where no arable land is needed and which is faster and more efficient.
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          <a class="in-text-link" href="https://2017.igem.org/Team:Amsterdam/Project" target="_blank">
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          This is the way
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          </a>
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          our team has chosen to go, using cyanobacteria to take up CO
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          <sub>
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          2
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          </sub>
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          and directly convert it into the valuable chemical
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          <span style="color: rgba(88, 190, 190, 1)">
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          FUMARATE
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          </span>
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        </p>
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      <div class="vertical-text-container-outer" id="home">
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      <div class="vertical-text-container">
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        <p class="vertical-text">
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        PROJECT
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        </p>
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      </div>
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    <div class="info-oval-container" id="home">
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      <div class="left-semi-circle">
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      <p>
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        <b>
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        Fumarate
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        </b>
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        is used to make certain plastics, food additives, and medicine and is currently made from petroleum. The global market size of fumarate is
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        <a class="in-text-link" href="https://www.radiantinsights.com/press-release/global-fumaric-acid-market" target="_blank">
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        estimated
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        </a>
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        to exceed $760 million by 2020!
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      </p>
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      <div class="right-semi-circle">
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        <div class="home-intro-container" id="fifth">
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        <img src="https://static.igem.org/mediawiki/2017/d/d0/TAmsterdam_team_zwart.png"/>
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        <p>
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          We are 6 students from Amsterdam with a passion for creating a more sustainable future.
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        </p>
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      <div class="home-content-container">
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        <img src="https://static.igem.org/mediawiki/2017/6/66/TAmsterdam_practices.png"/>
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        <p>
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          We joined iGEM because we did not just wanted to ‘do science’ but we wanted to reach out to society, learn about communicating our research, and work on a shared vision in a small dynamic team.
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        </p>
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        <div class="home-intro-container" id="fifth">
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        <img src="https://static.igem.org/mediawiki/2017/9/9a/TAmsterdam_collaboration.png"/>
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        <p>
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          Guided by our fantastic supervisors, and owing to the generosity of our sponsors, we have managed to successfully conclude this challenging and incredibly rewarding project.
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        </p>
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        </div>
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      </div>
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      <div class="home-content-container">
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        <img src="https://static.igem.org/mediawiki/2017/f/f3/TAmsterdam_lab.png"/>
 +
        <p>
 +
          We want to especially thank the Molecular Microbial Physiology group at the University of Amsterdam who have welcomed us in their lab for the past 7 months.
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        </p>
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      <div class="logo-spacer alternate">
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        <img src="https://static.igem.org/mediawiki/2017/1/1a/TAmsterdam_logo_line1.png"/>
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    <div class="home-gif-container">
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      <img src="https://static.igem.org/mediawiki/2017/c/c8/TAmsterdam_logo_gif.gif"/>
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<img src='images/design_thingy_1.png'>
 
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<div class="summary-container">
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<div class="summary-col-left">
  
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<div class="summary-col-mid">
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<p class="summary-text">We are running out of oil and the climate is changing drastically due to the emission of polluting gases such as CO<sub>2</sub>. But what if CO<sub>2</sub> were a resource, rather than a wasteful pollutant; and might even replace oil? We aspire to create a bio-based economy – one that meets its energy and production demands by leveraging biotechnology [1]. We can genetically engineer bacteria to produce a compound we need. This is what we call a bacterial ‘cell factory’.
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<h1> Welcome to iGEM 2017! </h1>
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Meet <a class="in-text-link" href="#" style="font-size: 24px">CYANOFACTORY</a>, a cyanobacterium that stably and efficiently produces fumarate.</p>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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</div>
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<div class="summary-col-right">
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<div class="clear"></div>
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<p class='vertical-text'>OUR GOAL</p>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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</ul>
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</div>
 
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<div class="home-spacer">
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<div class="column half_size" >
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<img src='images/design_thingy_1.png'>
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<div class="highlight">
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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</div>
 
</div>
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<div class="summary-container">
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<div class="summary-col-left">
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<img src="images/erlenmeyer_flask.png" width="200px" height="260px">
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</div>
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<div class="summary-col-mid">
 +
<p class="summary-text">We use the cyanobacterium Synechocystis PCC6803, a photosynthetic model organism of which the genetic toolbox is rapidly expanding.<br><br>
 +
<a class="in-text-link" href="#">Click here for a live feed with our Synechocystis cultivators.</a></p>
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</div>
 +
<div class="summary-col-right">
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<div class="vertical-text-container">
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<p class='vertical-text'>SYNECHOCYSTIS</p>
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<img src='images/design_thingy_1.png'>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<div class="column half_size" >
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<div class="summary-container">
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<img src="images/fumarate.png" width="200px" height="202px">
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<div class="summary-col-mid">
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<ul>
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<p class="summary-text">Fumarate is a multifaceted acid that is used as a plastic precursor, an additive for the food industry and a drug against multiple sclerosis and psoriasis.<br><br>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
<a class="in-text-link" href="#">Click here to see what we did with Fumarate.</a></p>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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</div>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<div class="summary-col-right">
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<div class="vertical-text-container">
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<p class='vertical-text'>FUMARATE</p>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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</div>
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<li>Have lots of fun! </li>
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</div>
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</ul>  
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</div>
 
</div>
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<div id="modules" class="summary-container">
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<div id="modules" class="home-spacer">
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<img src='images/design_thingy_1_long.png'>
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</div>
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<div class="summary-col-left">
  
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<div class="column half_size" >
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<div class="summary-col-mid">
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<h5>Inspiration</h5>
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<div id='sensor' class="summary-modules-outer">
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<img class="animated-module-icon" src='images/sensor_icon.png'>
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<ul>
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<div class="modules-summary-text">
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<h1>Biosensor</h1>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
<p>A biosensor for fumarate is constructed in order to facilitate a high throughput screening of extracellular fumarate. This is essential for low measurement time and costs.
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
<br><a class="in-text-link" href="#">>>read more</a></p>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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</div>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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</div>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<div id="transporter" class="summary-modules-outer">
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</ul>
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<img class="animated-module-icon" src='images/transporter_icon.png'>
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<div class="modules-summary-text">
 +
<h1>Transporter</h1>
 +
<p>In anticipation of high fumarate production, transport of fumarate out of the cell can be a limiting factor. This mechanism is largely unknown in Synechocystis and will therefore be -guided by bioinformatics- characterized by means of knock-out and over- expression experiments.
 +
<br><a class="in-text-link" href="#">>>read more</a></p>
 +
</div>
 +
</div>
 +
<div id="shunt" class="summary-modules-outer">
 +
<img class="animated-module-icon" src='images/shunt_icon.png'>
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<div class="modules-summary-text">
 +
<h1>Glyoxylate Shunt</h1>
 +
<p>We will incorporate two enzymes (the glyoxylate shunt) in the Synechocystis genome, creating a shortcut in the TCA-cycle. Our modeling results show that if this shunt is only expressed at night, and the pentose phosphate pathway is blocked by knocking out the zwf gene, more fumarate will be produced. This means we will create a unique combination of growth- coupled and inducible production.
 +
<br><a class="in-text-link" href="#">>>read more</a></p>
 +
</div>
 +
</div>
 +
<div id="stability" class="summary-modules-outer">
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<img class="animated-module-icon" src='images/stability_icon.png'>
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<div class="modules-summary-text">
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<h1>Stability</h1>
 +
<p>When an organism is genetically modified to create a product, it causes a decrease in growth rate. This means that a mutated - non- producing - organism will grow faster and take over the population. This problem is tackled by coupling fumarate production to growth. Given that evolution selects heavily on growth rate, we now have a way of naturally selecting for production rate [4]! Knocking out the fumarate degrading reaction in the TCA cycle causes fumarate to be produced in a growth coupled way.
 +
<br><a class="in-text-link" href="#>">>>read more</a></p>
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</div>
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</div>
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</div>
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<div class="summary-col-right">
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<div class="vertical-text-container">
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<p class='vertical-text'>MODULES</p>
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</div>
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</div>
 
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<h5> Uploading pictures and files </h5>
 
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
 
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<a href="https://2017.igem.org/Special:Upload">
 
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UPLOAD FILES
 
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Latest revision as of 11:25, 23 November 2017

Home

Project

Project

Practices

Practices

Lab

Lab

Team

Team

Achievements

Achievements

We are running out of oil and the climate is changing drastically due to the emission of greenhouse gases such as CO 2 .

But what if CO 2 were a resource, rather than a wasteful pollutant; and might even replace oil?

Early attempts to achieve this have focused on producing sugars with plants, which are then subsequently fermented by bacteria to produce liquid fuels and other chemicals.

But there is a better way, where no arable land is needed and which is faster and more efficient. This is the way our team has chosen to go, using cyanobacteria to take up CO 2 and directly convert it into the valuable chemical FUMARATE

PROJECT

Fumarate is used to make certain plastics, food additives, and medicine and is currently made from petroleum. The global market size of fumarate is estimated to exceed $760 million by 2020!

We are 6 students from Amsterdam with a passion for creating a more sustainable future.

We joined iGEM because we did not just wanted to ‘do science’ but we wanted to reach out to society, learn about communicating our research, and work on a shared vision in a small dynamic team.

Guided by our fantastic supervisors, and owing to the generosity of our sponsors, we have managed to successfully conclude this challenging and incredibly rewarding project.

We want to especially thank the Molecular Microbial Physiology group at the University of Amsterdam who have welcomed us in their lab for the past 7 months.

Auf Gss Aimms1 Sciencepark Dsm Idt Matrix Merck Rivm Phenomenex Snapgene Sils United consumers Vu Vu fs Vu cs