Difference between revisions of "Team:Amsterdam"
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| − | + | <meta content="width=device-width, initial-scale=1, shrink-to-fit=no" name="viewport"/> | |
| − | + | <title> | |
| − | + | Home | |
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| − | + | <link href="https://2017.igem.org/Team:Amsterdam/assets/css/normalize?action=raw&ctype=text/css" rel="stylesheet"/> | |
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| − | + | <link href="https://2017.igem.org/Team:Amsterdam/assets/css/all?action=raw&ctype=text/css" rel="stylesheet"/> | |
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| − | + | <script src="https://2017.igem.org/Team:Amsterdam/assets/js/all?action=raw&ctype=text/javascript"> | |
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| − | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/8/86/TAmsterdam_pm_logo.png"/> |
| − | + | <a class="navbar-brand nav-link" href="https://2017.igem.org/Team:Amsterdam"> | |
| − | + | Photosynthetic Magic | |
| − | + | </a> | |
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</div> | </div> | ||
<ul class="list-inline nav navbar-right"> | <ul class="list-inline nav navbar-right"> | ||
<li class="list-inline-item dropdown"> | <li class="list-inline-item dropdown"> | ||
| − | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/ | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Project"> |
Project | Project | ||
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| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Project"> |
| − | + | Overview | |
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Produce"> |
| − | + | Production | |
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Export"> |
| − | + | Exportation | |
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Detect"> |
| − | + | Detection | |
</a> | </a> | ||
</li> | </li> | ||
</ul> | </ul> | ||
| + | </li> | ||
| + | <li class="list-inline-item"> | ||
| + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Model"> | ||
| + | Model | ||
| + | </a> | ||
</li> | </li> | ||
<li class="list-inline-item dropdown"> | <li class="list-inline-item dropdown"> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Practices"> |
Practices | Practices | ||
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<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Practices"> |
| + | Overview | ||
| + | </a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Carbon"> | ||
Carbon Efficiency | Carbon Efficiency | ||
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/ | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/HP/Silver"> |
| − | + | HP Silver | |
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/HP/Gold_Integrated"> |
| − | + | HP Gold | |
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Engagement"> |
| + | Engagement | ||
| + | </a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Safety"> | ||
Safety | Safety | ||
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| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Lab"> |
Lab | Lab | ||
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| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Lab"> |
| + | Overview | ||
| + | </a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Parts"> | ||
Parts | Parts | ||
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/ | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/InterLab"> |
Interlab Study | Interlab Study | ||
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/LabJournal"> |
Lab Journal | Lab Journal | ||
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Protocol"> |
| − | + | Protocols | |
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Methods"> |
Methods | Methods | ||
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| − | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/ | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Team"> |
Team | Team | ||
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<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li> | <li> | ||
| − | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/ | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Team"> |
| + | Overview | ||
| + | </a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Attributions"> | ||
Attributions | Attributions | ||
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Collaborations"> |
Collaborations | Collaborations | ||
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/ | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Sponsors"> |
Sponsors | Sponsors | ||
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<li class="list-inline-item"> | <li class="list-inline-item"> | ||
| − | <a class="nav-link" href=" | + | <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Achievements"> |
Achievements | Achievements | ||
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</nav> | </nav> | ||
<div class="inner cover"> | <div class="inner cover"> | ||
| − | <div | + | <div class="video-container"> |
<video controls="" height="auto" poster="https://static.igem.org/mediawiki/2017/8/8f/Intro_poster.png" width="85%"> | <video controls="" height="auto" poster="https://static.igem.org/mediawiki/2017/8/8f/Intro_poster.png" width="85%"> | ||
| − | <source src="https://static.igem.org/mediawiki/2017/ | + | <source src="https://static.igem.org/mediawiki/2017/4/4c/TAmsterdam_intro.mp4" type="video/mp4"/> |
| − | + | ||
</video> | </video> | ||
</div> | </div> | ||
<div class="section-button-container"> | <div class="section-button-container"> | ||
| − | <a class="section-button" href=" | + | <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Project" id="Project"> |
| − | <img alt="Project" src="https://static.igem.org/mediawiki/2017/9/ | + | <img alt="Project" src="https://static.igem.org/mediawiki/2017/9/9b/TAmsterdam_project.png"> |
| − | + | <p> | |
| − | + | Project | |
| − | + | </p> | |
| + | </img> | ||
</a> | </a> | ||
| − | <a class="section-button" href=" | + | <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Practices" id="Practices"> |
| − | <img alt="Practices" src="https://static.igem.org/mediawiki/2017/6/ | + | <img alt="Practices" src="https://static.igem.org/mediawiki/2017/6/66/TAmsterdam_practices.png"/> |
<p> | <p> | ||
Practices | Practices | ||
</p> | </p> | ||
</a> | </a> | ||
| − | <a class="section-button" href=" | + | <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Lab" id="Lab"> |
| − | <img alt="Lab" src="https://static.igem.org/mediawiki/2017/ | + | <img alt="Lab" src="https://static.igem.org/mediawiki/2017/f/f3/TAmsterdam_lab.png"/> |
<p> | <p> | ||
Lab | Lab | ||
</p> | </p> | ||
</a> | </a> | ||
| − | <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/ | + | <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Team" id="Team"> |
| − | <img alt="Team" src="https://static.igem.org/mediawiki/2017/ | + | <img alt="Team" src="https://static.igem.org/mediawiki/2017/0/06/TAmsterdam_team.png"/> |
<p> | <p> | ||
Team | Team | ||
</p> | </p> | ||
</a> | </a> | ||
| − | <a class="section-button" href=" | + | <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Achievements" id="Achievements"> |
| − | <img alt="Achievements" src="https://static.igem.org/mediawiki/2017/ | + | <img alt="Achievements" src="https://static.igem.org/mediawiki/2017/9/94/TAmsterdam_achievements.png"/> |
<p> | <p> | ||
Achievements | Achievements | ||
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<div class="home-project-container-outer"> | <div class="home-project-container-outer"> | ||
<div class="home-project-container"> | <div class="home-project-container"> | ||
| + | <div class="logo-spacer alternate"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/1/1a/TAmsterdam_logo_line1.png"/> | ||
| + | </div> | ||
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| − | <img src="https://static.igem.org/mediawiki/2017/6/ | + | <img src="https://static.igem.org/mediawiki/2017/6/66/TAmsterdam_practices.png"/> |
| − | + | <p> | |
| − | + | We are running out of oil and the climate is changing drastically due to the emission of greenhouse gases such as CO | |
| − | </ | + | <sub> |
| − | </ | + | 2 |
| + | </sub> | ||
| + | . | ||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
<div class="home-content-container"> | <div class="home-content-container"> | ||
| − | <div class="line-connector" id=" | + | <div class="line-connector" id="second"> |
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| − | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/e/ed/TAmsterdam_recycle.png"/> |
| − | + | <p> | |
| − | + | But what if CO | |
| − | + | <sub> | |
| − | </ | + | 2 |
| + | </sub> | ||
| + | were a resource, rather than a wasteful pollutant; and might even replace oil? | ||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
<div class="line-connector-container"> | <div class="line-connector-container"> | ||
| − | <div class="line-connector" id=" | + | <div class="line-connector" id="third"> |
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<div class="home-content-container"> | <div class="home-content-container"> | ||
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| − | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/8/8a/TAmsterdam_plants.png"/> |
| − | + | <p> | |
| − | + | Early attempts to achieve this have focused on producing sugars with plants, which are then subsequently fermented by bacteria to produce liquid fuels and other chemicals. | |
| − | + | </p> | |
| − | + | ||
</div> | </div> | ||
</div> | </div> | ||
<div class="home-content-container"> | <div class="home-content-container"> | ||
| − | <div class="line-connector" id=" | + | <div class="line-connector" id="second"> |
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<div class="home-intro-container" id="second"> | <div class="home-intro-container" id="second"> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/9/ | + | <img src="https://static.igem.org/mediawiki/2017/9/9b/TAmsterdam_project.png"/> |
| − | + | <p> | |
| − | + | But there is a better way, where no arable land is needed and which is faster and more efficient. | |
| − | + | <a class="in-text-link" href="https://2017.igem.org/Team:Amsterdam/Project" target="_blank"> | |
| − | + | This is the way | |
| − | + | </a> | |
| − | + | our team has chosen to go, using cyanobacteria to take up CO | |
| − | + | <sub> | |
| + | 2 | ||
| + | </sub> | ||
| + | and directly convert it into the valuable chemical | ||
| + | <span style="color: rgba(88, 190, 190, 1)"> | ||
| + | FUMARATE | ||
| + | </span> | ||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
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<p> | <p> | ||
<b> | <b> | ||
| − | + | Fumarate | |
</b> | </b> | ||
| − | + | is used to make certain plastics, food additives, and medicine and is currently made from petroleum. The global market size of fumarate is | |
| − | + | <a class="in-text-link" href="https://www.radiantinsights.com/press-release/global-fumaric-acid-market" target="_blank"> | |
| − | </ | + | estimated |
| + | </a> | ||
| + | to exceed $760 million by 2020! | ||
</p> | </p> | ||
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| + | <div class="home-project-container-outer"> | ||
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| + | </div> | ||
| + | </div> | ||
| + | <div class="home-content-container"> | ||
| + | <div class="home-intro-container" id="fifth"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/d/d0/TAmsterdam_team_zwart.png"/> | ||
| + | <p> | ||
| + | We are 6 students from Amsterdam with a passion for creating a more sustainable future. | ||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="home-content-container"> | ||
| + | <div class="line-connector" id="second"> | ||
| + | </div> | ||
| + | <div class="home-intro-container" id="second"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/6/66/TAmsterdam_practices.png"/> | ||
| + | <p> | ||
| + | We joined iGEM because we did not just wanted to ‘do science’ but we wanted to reach out to society, learn about communicating our research, and work on a shared vision in a small dynamic team. | ||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="line-connector-container"> | ||
| + | <div class="line-connector" id="sixth"> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="line-connector-container"> | ||
| + | <div class="line-connector" id="seventh"> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="home-content-container"> | ||
| + | <div class="home-intro-container" id="fifth"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/9/9a/TAmsterdam_collaboration.png"/> | ||
| + | <p> | ||
| + | Guided by our fantastic supervisors, and owing to the generosity of our sponsors, we have managed to successfully conclude this challenging and incredibly rewarding project. | ||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="home-content-container"> | ||
| + | <div class="line-connector" id="second"> | ||
| + | </div> | ||
| + | <div class="home-intro-container" id="second"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/f/f3/TAmsterdam_lab.png"/> | ||
| + | <p> | ||
| + | We want to especially thank the Molecular Microbial Physiology group at the University of Amsterdam who have welcomed us in their lab for the past 7 months. | ||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="line-connector-container"> | ||
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| + | </div> | ||
| + | <div class="logo-spacer alternate"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/1/1a/TAmsterdam_logo_line1.png"/> | ||
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
<div class="home-gif-container"> | <div class="home-gif-container"> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/c/c8/TAmsterdam_logo_gif.gif"/> |
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<!-- <div class="home-spacer"> | <!-- <div class="home-spacer"> | ||
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<div class="summary-col-left"> | <div class="summary-col-left"> | ||
| − | + | ||
</div> | </div> | ||
<div class="summary-col-mid"> | <div class="summary-col-mid"> | ||
| − | <p class="summary-text">We are running out of oil and the climate is changing drastically due to the emission of polluting gases such as CO<sub>2</sub>. But what if CO<sub>2</sub> were a resource, rather than a wasteful pollutant; and might even replace oil? We aspire to create a bio-based economy – one that meets its energy and production demands by leveraging biotechnology [1]. We can genetically engineer bacteria to produce a compound we need. This is what we call a bacterial ‘cell factory’. | + | <p class="summary-text">We are running out of oil and the climate is changing drastically due to the emission of polluting gases such as CO<sub>2</sub>. But what if CO<sub>2</sub> were a resource, rather than a wasteful pollutant; and might even replace oil? We aspire to create a bio-based economy – one that meets its energy and production demands by leveraging biotechnology [1]. We can genetically engineer bacteria to produce a compound we need. This is what we call a bacterial ‘cell factory’. |
Meet <a class="in-text-link" href="#" style="font-size: 24px">CYANOFACTORY</a>, a cyanobacterium that stably and efficiently produces fumarate.</p> | Meet <a class="in-text-link" href="#" style="font-size: 24px">CYANOFACTORY</a>, a cyanobacterium that stably and efficiently produces fumarate.</p> | ||
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<h1>Glyoxylate Shunt</h1> | <h1>Glyoxylate Shunt</h1> | ||
| − | <p>We will incorporate two enzymes (the glyoxylate shunt) in the Synechocystis genome, creating a shortcut in the TCA-cycle. Our modeling results show that if this shunt is only expressed at night, and the pentose phosphate pathway is blocked by knocking out the zwf gene, more fumarate will be produced. This means we will create a unique combination of growth- coupled and inducible production. | + | <p>We will incorporate two enzymes (the glyoxylate shunt) in the Synechocystis genome, creating a shortcut in the TCA-cycle. Our modeling results show that if this shunt is only expressed at night, and the pentose phosphate pathway is blocked by knocking out the zwf gene, more fumarate will be produced. This means we will create a unique combination of growth- coupled and inducible production. |
<br><a class="in-text-link" href="#">>>read more</a></p> | <br><a class="in-text-link" href="#">>>read more</a></p> | ||
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Latest revision as of 11:25, 23 November 2017
We are running out of oil and the climate is changing drastically due to the emission of greenhouse gases such as CO 2 .
But what if CO 2 were a resource, rather than a wasteful pollutant; and might even replace oil?
Early attempts to achieve this have focused on producing sugars with plants, which are then subsequently fermented by bacteria to produce liquid fuels and other chemicals.
But there is a better way, where no arable land is needed and which is faster and more efficient. This is the way our team has chosen to go, using cyanobacteria to take up CO 2 and directly convert it into the valuable chemical FUMARATE
PROJECT
Fumarate is used to make certain plastics, food additives, and medicine and is currently made from petroleum. The global market size of fumarate is estimated to exceed $760 million by 2020!
We are 6 students from Amsterdam with a passion for creating a more sustainable future.
We joined iGEM because we did not just wanted to ‘do science’ but we wanted to reach out to society, learn about communicating our research, and work on a shared vision in a small dynamic team.
Guided by our fantastic supervisors, and owing to the generosity of our sponsors, we have managed to successfully conclude this challenging and incredibly rewarding project.
We want to especially thank the Molecular Microbial Physiology group at the University of Amsterdam who have welcomed us in their lab for the past 7 months.
