Difference between revisions of "Team:Amsterdam"

 
(42 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
{{Amsterdam}}
 
−
 
 
<html>
 
<html>
 +
<head>
 +
  <meta charset="utf-8"/>
 +
  <meta content="ie=edge" http-equiv="x-ua-compatible"/>
 +
  <meta content="width=device-width, initial-scale=1, shrink-to-fit=no" name="viewport"/>
 +
  <title>
 +
  Home
 +
  </title>
 +
  <!-- include normalizer -->
 +
  <link href="https://2017.igem.org/Team:Amsterdam/assets/css/normalize?action=raw&amp;ctype=text/css" rel="stylesheet"/>
 +
  <!-- include auto generated css -->
 +
  <link href="https://2017.igem.org/Team:Amsterdam/assets/css/all?action=raw&amp;ctype=text/css" rel="stylesheet"/>
 +
  <!-- include auto generated javascript -->
 +
  <script src="https://2017.igem.org/Team:Amsterdam/assets/js/all?action=raw&amp;ctype=text/javascript">
 +
  </script>
 +
  <!-- include mathjax -->
 +
  <script src="https://cdnjs.cloudflare.com/ajax/libs/mathjax/2.7.2/MathJax.js?config=TeX-MML-AM_CHTML">
 +
  </script>
 +
</head>
 +
<div class="site-wrapper">
 +
  <div class="site-wrapper-inner">
 +
  <div class="cover-container">
 +
    <nav class="navbar navbar-inverse">
 +
    <div class="container-fluid">
 +
      <div class="navbar-header">
 +
      <img src="https://static.igem.org/mediawiki/2017/8/86/TAmsterdam_pm_logo.png"/>
 +
      <a class="navbar-brand nav-link" href="https://2017.igem.org/Team:Amsterdam">
 +
        Photosynthetic Magic
 +
      </a>
 +
      </div>
 +
      <ul class="list-inline nav navbar-right">
 +
      <li class="list-inline-item dropdown">
 +
        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Project">
 +
        Project
 +
        <span class="caret">
 +
        </span>
 +
        </a>
 +
        <ul class="dropdown-menu">
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Project">
 +
          Overview
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Produce">
 +
          Production
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Export">
 +
          Exportation
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Detect">
 +
          Detection
 +
          </a>
 +
        </li>
 +
        </ul>
 +
      </li>
 +
      <li class="list-inline-item">
 +
        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Model">
 +
        Model
 +
        </a>
 +
      </li>
 +
      <li class="list-inline-item dropdown">
 +
        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Practices">
 +
        Practices
 +
        <span class="caret">
 +
        </span>
 +
        </a>
 +
        <ul class="dropdown-menu">
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Practices">
 +
          Overview
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Carbon">
 +
          Carbon Efficiency
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/HP/Silver">
 +
          HP Silver
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/HP/Gold_Integrated">
 +
          HP Gold
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Engagement">
 +
          Engagement
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Safety">
 +
          Safety
 +
          </a>
 +
        </li>
 +
        </ul>
 +
      </li>
 +
      <li class="list-inline-item dropdown">
 +
        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Lab">
 +
        Lab
 +
        <span class="caret">
 +
        </span>
 +
        </a>
 +
        <ul class="dropdown-menu">
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Lab">
 +
          Overview
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Parts">
 +
          Parts
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/InterLab">
 +
          Interlab Study
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/LabJournal">
 +
          Lab Journal
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Protocol">
 +
          Protocols
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Methods">
 +
          Methods
 +
          </a>
 +
        </li>
 +
        </ul>
 +
      </li>
 +
      <li class="list-inline-item dropdown">
 +
        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Team">
 +
        Team
 +
        <span class="caret">
 +
        </span>
 +
        </a>
 +
        <ul class="dropdown-menu">
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Team">
 +
          Overview
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Attributions">
 +
          Attributions
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Collaborations">
 +
          Collaborations
 +
          </a>
 +
        </li>
 +
        <li>
 +
          <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Sponsors">
 +
          Sponsors
 +
          </a>
 +
        </li>
 +
        </ul>
 +
      </li>
 +
      <li class="list-inline-item">
 +
        <a class="nav-link" href="https://2017.igem.org/Team:Amsterdam/Achievements">
 +
        Achievements
 +
        </a>
 +
      </li>
 +
      </ul>
 +
    </div>
 +
    </nav>
 +
    <div class="inner cover">
 +
    <div class="video-container">
 +
      <video controls="" height="auto" poster="https://static.igem.org/mediawiki/2017/8/8f/Intro_poster.png" width="85%">
 +
      <source src="https://static.igem.org/mediawiki/2017/4/4c/TAmsterdam_intro.mp4" type="video/mp4"/>
 +
      </video>
 +
    </div>
 +
    <div class="section-button-container">
 +
      <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Project" id="Project">
 +
      <img alt="Project" src="https://static.igem.org/mediawiki/2017/9/9b/TAmsterdam_project.png">
 +
        <p>
 +
        Project
 +
        </p>
 +
      </img>
 +
      </a>
 +
      <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Practices" id="Practices">
 +
      <img alt="Practices" src="https://static.igem.org/mediawiki/2017/6/66/TAmsterdam_practices.png"/>
 +
      <p>
 +
        Practices
 +
      </p>
 +
      </a>
 +
      <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Lab" id="Lab">
 +
      <img alt="Lab" src="https://static.igem.org/mediawiki/2017/f/f3/TAmsterdam_lab.png"/>
 +
      <p>
 +
        Lab
 +
      </p>
 +
      </a>
 +
      <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Team" id="Team">
 +
      <img alt="Team" src="https://static.igem.org/mediawiki/2017/0/06/TAmsterdam_team.png"/>
 +
      <p>
 +
        Team
 +
      </p>
 +
      </a>
 +
      <a class="section-button" href="https://2017.igem.org/Team:Amsterdam/Achievements" id="Achievements">
 +
      <img alt="Achievements" src="https://static.igem.org/mediawiki/2017/9/94/TAmsterdam_achievements.png"/>
 +
      <p>
 +
        Achievements
 +
      </p>
 +
      </a>
 +
    </div>
 +
    <div class="home-project-container-outer">
 +
      <div class="home-project-container">
 +
      <div class="logo-spacer alternate">
 +
        <img src="https://static.igem.org/mediawiki/2017/1/1a/TAmsterdam_logo_line1.png"/>
 +
      </div>
 +
      <div class="line-connector-container">
 +
        <div class="line-connector" id="first">
 +
        </div>
 +
      </div>
 +
      <div class="home-content-container">
 +
        <div class="home-intro-container" id="first">
 +
        <img src="https://static.igem.org/mediawiki/2017/6/66/TAmsterdam_practices.png"/>
 +
        <p>
 +
          We are running out of oil and the climate is changing drastically due to the emission of greenhouse gases such as CO
 +
          <sub>
 +
          2
 +
          </sub>
 +
          .
 +
        </p>
 +
        </div>
 +
      </div>
 +
      <div class="home-content-container">
 +
        <div class="line-connector" id="second">
 +
        </div>
 +
        <div class="home-intro-container" id="second">
 +
        <img src="https://static.igem.org/mediawiki/2017/e/ed/TAmsterdam_recycle.png"/>
 +
        <p>
 +
          But what if CO
 +
          <sub>
 +
          2
 +
          </sub>
 +
          were a resource, rather than a wasteful pollutant; and might even replace oil?
 +
        </p>
 +
        </div>
 +
      </div>
 +
      <div class="line-connector-container">
 +
        <div class="line-connector" id="third">
 +
        </div>
 +
      </div>
 +
      <div class="home-content-container">
 +
        <div class="home-intro-container" id="first">
 +
        <img src="https://static.igem.org/mediawiki/2017/8/8a/TAmsterdam_plants.png"/>
 +
        <p>
 +
          Early attempts to achieve this have focused on producing sugars with plants, which are then subsequently fermented by bacteria to produce liquid fuels and other chemicals.
 +
        </p>
 +
        </div>
 +
      </div>
 +
      <div class="home-content-container">
 +
        <div class="line-connector" id="second">
 +
        </div>
 +
        <div class="home-intro-container" id="second">
 +
        <img src="https://static.igem.org/mediawiki/2017/9/9b/TAmsterdam_project.png"/>
 +
        <p>
 +
          But there is a better way, where no arable land is needed and which is faster and more efficient.
 +
          <a class="in-text-link" href="https://2017.igem.org/Team:Amsterdam/Project" target="_blank">
 +
          This is the way
 +
          </a>
 +
          our team has chosen to go, using cyanobacteria to take up CO
 +
          <sub>
 +
          2
 +
          </sub>
 +
          and directly convert it into the valuable chemical
 +
          <span style="color: rgba(88, 190, 190, 1)">
 +
          FUMARATE
 +
          </span>
 +
        </p>
 +
        </div>
 +
      </div>
 +
      <div class="line-connector-container">
 +
        <div class="line-connector" id="third">
 +
        </div>
 +
      </div>
 +
      </div>
 +
      <div class="vertical-text-container-outer" id="home">
 +
      <div class="vertical-text-container">
 +
        <p class="vertical-text">
 +
        PROJECT
 +
        </p>
 +
      </div>
 +
      </div>
 +
    </div>
 +
    <div class="info-oval-container" id="home">
 +
      <div class="line-connector-container">
 +
      <div class="line-connector" id="fourth">
 +
      </div>
 +
      </div>
 +
      <div class="info-oval">
 +
      <div class="left-semi-circle">
 +
      </div>
 +
      <p>
 +
        <b>
 +
        Fumarate
 +
        </b>
 +
        is used to make certain plastics, food additives, and medicine and is currently made from petroleum. The global market size of fumarate is
 +
        <a class="in-text-link" href="https://www.radiantinsights.com/press-release/global-fumaric-acid-market" target="_blank">
 +
        estimated
 +
        </a>
 +
        to exceed $760 million by 2020!
 +
      </p>
 +
      <div class="right-semi-circle">
 +
      </div>
 +
      </div>
 +
      <div class="line-connector-container">
 +
      <div class="line-connector" id="fourth">
 +
      </div>
 +
      </div>
 +
    </div>
 +
    <div class="home-project-container-outer">
 +
      <div class="home-project-container">
 +
      <div class="line-connector-container">
 +
        <div class="line-connector" id="third">
 +
        </div>
 +
      </div>
 +
      <div class="line-connector-container">
 +
        <div class="line-connector" id="fifth">
 +
        </div>
 +
      </div>
 +
      <div class="home-content-container">
 +
        <div class="home-intro-container" id="fifth">
 +
        <img src="https://static.igem.org/mediawiki/2017/d/d0/TAmsterdam_team_zwart.png"/>
 +
        <p>
 +
          We are 6 students from Amsterdam with a passion for creating a more sustainable future.
 +
        </p>
 +
        </div>
 +
      </div>
 +
      <div class="home-content-container">
 +
        <div class="line-connector" id="second">
 +
        </div>
 +
        <div class="home-intro-container" id="second">
 +
        <img src="https://static.igem.org/mediawiki/2017/6/66/TAmsterdam_practices.png"/>
 +
        <p>
 +
          We joined iGEM because we did not just wanted to ‘do science’ but we wanted to reach out to society, learn about communicating our research, and work on a shared vision in a small dynamic team.
 +
        </p>
 +
        </div>
 +
      </div>
 +
      <div class="line-connector-container">
 +
        <div class="line-connector" id="sixth">
 +
        </div>
 +
      </div>
 +
      <div class="line-connector-container">
 +
        <div class="line-connector" id="seventh">
 +
        </div>
 +
      </div>
 +
      <div class="home-content-container">
 +
        <div class="home-intro-container" id="fifth">
 +
        <img src="https://static.igem.org/mediawiki/2017/9/9a/TAmsterdam_collaboration.png"/>
 +
        <p>
 +
          Guided by our fantastic supervisors, and owing to the generosity of our sponsors, we have managed to successfully conclude this challenging and incredibly rewarding project.
 +
        </p>
 +
        </div>
 +
      </div>
 +
      <div class="home-content-container">
 +
        <div class="line-connector" id="second">
 +
        </div>
 +
        <div class="home-intro-container" id="second">
 +
        <img src="https://static.igem.org/mediawiki/2017/f/f3/TAmsterdam_lab.png"/>
 +
        <p>
 +
          We want to especially thank the Molecular Microbial Physiology group at the University of Amsterdam who have welcomed us in their lab for the past 7 months.
 +
        </p>
 +
        </div>
 +
      </div>
 +
      <div class="line-connector-container">
 +
        <div class="line-connector" id="sixth">
 +
        </div>
 +
      </div>
 +
      <div class="line-connector-container" id="second">
 +
        <div class="line-connector" id="eight">
 +
        </div>
 +
      </div>
 +
      <div class="logo-spacer alternate">
 +
        <img src="https://static.igem.org/mediawiki/2017/1/1a/TAmsterdam_logo_line1.png"/>
 +
      </div>
 +
      </div>
 +
    </div>
 +
    <div class="home-gif-container">
 +
      <img src="https://static.igem.org/mediawiki/2017/c/c8/TAmsterdam_logo_gif.gif"/>
 +
    </div>
 +
    <!-- <div class="home-spacer">
 +
<img src='images/design_thingy_1.png'>
 +
</div>
 +
<div class="summary-container">
 +
<div class="summary-col-left">
  
−
<head>
+
</div>
−
<title>iGEM Amsterdam 2017</title>
+
<div class="summary-col-mid">
−
    <meta charset="utf-8">
+
<p class="summary-text">We are running out of oil and the climate is changing drastically due to the emission of polluting gases such as CO<sub>2</sub>. But what if CO<sub>2</sub> were a resource, rather than a wasteful pollutant; and might even replace oil? We aspire to create a bio-based economy – one that meets its energy and production demands by leveraging biotechnology [1]. We can genetically engineer bacteria to produce a compound we need. This is what we call a bacterial ‘cell factory’.
−
    <link rel="stylesheet" href="style.css">
+
Meet <a class="in-text-link" href="#" style="font-size: 24px">CYANOFACTORY</a>, a cyanobacterium that stably and efficiently produces fumarate.</p>
−
    <meta name="viewport" content="width=device-width , initial-scale=1.0">
+
</div>
−
</head>
+
<div class="summary-col-right">
 +
<div class="vertical-text-container">
 +
<p class='vertical-text'>OUR GOAL</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="home-spacer">
 +
<img src='images/design_thingy_1.png'>
 +
</div>
 +
<div class="summary-container">
 +
<div class="summary-col-left">
 +
<img src="images/erlenmeyer_flask.png" width="200px" height="260px">
 +
</div>
 +
<div class="summary-col-mid">
 +
<p class="summary-text">We use the cyanobacterium Synechocystis PCC6803, a photosynthetic model organism of which the genetic toolbox is rapidly expanding.<br><br>
 +
<a class="in-text-link" href="#">Click here for a live feed with our Synechocystis cultivators.</a></p>
 +
</div>
 +
<div class="summary-col-right">
 +
<div class="vertical-text-container">
 +
<p class='vertical-text'>SYNECHOCYSTIS</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="home-spacer">
 +
<img src='images/design_thingy_1.png'>
 +
</div>
 +
<div class="summary-container">
 +
<div class="summary-col-left">
 +
<img src="images/fumarate.png" width="200px" height="202px">
 +
</div>
 +
<div class="summary-col-mid">
 +
<p class="summary-text">Fumarate is a multifaceted acid that is used as a plastic precursor, an additive for the food industry and a drug against multiple sclerosis and psoriasis.<br><br>
 +
<a class="in-text-link" href="#">Click here to see what we did with Fumarate.</a></p>
 +
</div>
 +
<div class="summary-col-right">
 +
<div class="vertical-text-container">
 +
<p class='vertical-text'>FUMARATE</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div id="modules" class="summary-container">
 +
<div id="modules" class="home-spacer">
 +
<img src='images/design_thingy_1_long.png'>
 +
</div>
 +
<div class="summary-col-left">
  
−
 
−
<!-- //////////////////////////////////////////// -->
 
−
 
−
 
−
<header class="mainheader">
 
−
<img src="https://static.igem.org/mediawiki/2017/1/13/T_TeamAmsterdam_team.jpg">
 
−
 
−
</header>
 
−
 
−
<!-- //////////////////////////////////////////// -->
 
−
 
−
<div class="mainContant">
 
−
 
−
<section class="top-content">
 
−
<header>
 
−
<h2><a href="#"></a>iGEM Amsterdam</h2>
 
−
</header>
 
−
<article>
 
−
<h4>A new way of production</h4>
 
−
<p>
 
−
We are running out of oil and the climate is changing drastically due to the emission of greenhouse gasses such as carbon-dioxide. This is the world as we know it, but it is not the way it has to be. Imagine a world where carbon-dioxide is a useful resource instead of a wasteful pollutant. A resource that could even replace oil. We believe that this world is much closer than you might think, when we start exploiting the full potential of cell-factories. </p>
 
−
<p>
 
−
Bacteria as microscopic powerhouses
 
−
Many bacteria can be used to create so called cell-factories. The idea of a cell-factory is to exploit the native metabolism of a certain bacterium to let it produce a compound we need. This can be accomplished by creating a specific environment or by editing the genome of said bacterium. Organisms that are used most for this purpose are E.coli and yeast. These organisms do not use oil to produce valuable compounds, which is a large leap towards a bio-based economy!
 
−
 
−
 
−
Picky eaters
 
−
However, while chemoheterotrophic bacteria such as E.coli and yeast do not need oil to make products, they do need to eat. The food they need is glucose, or a similar complex carbon compound. Glucose is expensive, and the production of glucose is slow and takes up a lot of arable land. Furthermore, there are steps in the production of glucose which lead to additional efficiency loss, such as photosynthesis in plants and the creation of glucose from plant biomass. An ideal cell-factory would be able to skip all these steps and directly convert carbon-dioxide into a useful product.
 
−
 
−
Mean green CO2-eating machine
 
−
Bacteria that can live off carbon-dioxide have been around on our planet for a very long time, they are called cyanobacteria and are the guys that were the first to pump oxygen into the atmosphere billions of years ago! These bacteria are still around, and while we are less apt in editing their genome than we are with E.coli and yeast, we have come a long way. Using these bacteria we can indeed circumvent the efficiency loss and cost increase involved in glucose production, and directly convert carbon-dioxide into a useful product.
 
−
 
−
Continuous cultivation
 
−
Cyanobacteria only need carbon-dioxide and some salts to grow, which makes cultivation over longer periods of time an affordable and attractive option. When performing continuous cultivation you do not need to keep killing and re-inoculating batch cultures, and you will have continuous product formation.
 
−
 
−
Genetic instability
 
−
However, a big problem that comes into play with continuous cultivation is that of genetic instability. When an organism is genetically modified to create a product, resources need to be allocated towards product formation. If one of the modified organisms now obtains a mutation somewhere in the production pathway, these resources can be allocated towards growth. This means that the mutated - non-producing - organism will grow faster and take over the population.
 
−
 
−
 
−
Growth-coupling
 
−
This problem of genetic instability has been tackled before by team Amsterdam in 2015 and it entails coupling production to growth. This essentially means that an increase in growth inevitably leads to an increase in production. Because evolution selects heavily on growth rate, this is a parameter that is naturally optimised. By coupling production to growth we have a way of naturally selecting for production rate!
 
−
 
−
 
−
Producing fumarate
 
−
We have found a way to produce growth coupled fumarate in Synechocystis, a well-studied species of cyanobacteria. This is not just exciting because it is the first reported case of an autotrophic organism (organisms that do not need organic compounds to grow) being able to produce extracellular fumarate, but also because fumarate is a highly valued industrial commodity that is currently made from petroleum-based chemicals. We have achieved this by looking at team Amsterdam 2015’s growth coupling of acetate production, which inspired us to couple production of fumarate to growth in a similar manner. We will not stop here, but add to it by taking several approaches to incorporate a light dependent glyoxylate shunt within the TCA cycle to increase nighttime production of fumarate. This will lead to a unique combination of growth coupled production during the day and inducible production during the night.
 
−
 
−
 
−
 
−
Finishing touch
 
−
In order to be able to easily test how much fumarate our strains are producing, we will develop a fumarate biosensor for determining extracellular concentrations. And last but not least, in an attempt to face any future limitations on high fumarate production head-on, we are going to improve fumarate transport out of Synechocystis by integrating heterologous fumarate transport systems.                                         
 
−
                                                   
 
−
</p>
 
−
</article>
 
−
</section>
 
 
</div>
 
</div>
−
+
<div class="summary-col-mid">
−
</body>
+
<div id='sensor' class="summary-modules-outer">
 +
<img class="animated-module-icon" src='images/sensor_icon.png'>
 +
<div class="modules-summary-text">
 +
<h1>Biosensor</h1>
 +
<p>A biosensor for fumarate is constructed in order to facilitate a high throughput screening of extracellular fumarate. This is essential for low measurement time and costs.
 +
<br><a class="in-text-link" href="#">>>read more</a></p>
 +
</div>
 +
</div>
 +
<div id="transporter" class="summary-modules-outer">
 +
<img class="animated-module-icon" src='images/transporter_icon.png'>
 +
<div class="modules-summary-text">
 +
<h1>Transporter</h1>
 +
<p>In anticipation of high fumarate production, transport of fumarate out of the cell can be a limiting factor. This mechanism is largely unknown in Synechocystis and will therefore be -guided by bioinformatics- characterized by means of knock-out and over- expression experiments.
 +
<br><a class="in-text-link" href="#">>>read more</a></p>
 +
</div>
 +
</div>
 +
<div id="shunt" class="summary-modules-outer">
 +
<img class="animated-module-icon" src='images/shunt_icon.png'>
 +
<div class="modules-summary-text">
 +
<h1>Glyoxylate Shunt</h1>
 +
<p>We will incorporate two enzymes (the glyoxylate shunt) in the Synechocystis genome, creating a shortcut in the TCA-cycle. Our modeling results show that if this shunt is only expressed at night, and the pentose phosphate pathway is blocked by knocking out the zwf gene, more fumarate will be produced. This means we will create a unique combination of growth- coupled and inducible production.
 +
<br><a class="in-text-link" href="#">>>read more</a></p>
 +
</div>
 +
</div>
 +
<div id="stability" class="summary-modules-outer">
 +
<img class="animated-module-icon" src='images/stability_icon.png'>
 +
<div class="modules-summary-text">
 +
<h1>Stability</h1>
 +
<p>When an organism is genetically modified to create a product, it causes a decrease in growth rate. This means that a mutated - non- producing - organism will grow faster and take over the population. This problem is tackled by coupling fumarate production to growth. Given that evolution selects heavily on growth rate, we now have a way of naturally selecting for production rate [4]! Knocking out the fumarate degrading reaction in the TCA cycle causes fumarate to be produced in a growth coupled way.
 +
<br><a class="in-text-link" href="#>">>>read more</a></p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="summary-col-right">
 +
<div class="vertical-text-container">
 +
<p class='vertical-text'>MODULES</p>
 +
</div>
 +
</div>
 +
</div>
  
  
−
<style>
 
−
/*................MAIN HEADER.....................*/
 
−
 
−
.mainheader img {
 
−
width: 100%
 
−
}
 
−
 
−
 
−
 
−
/*................MAIN CONTENT.....................*/
 
−
 
−
.mainContent {
 
−
width: 70%;
 
−
float: left;
 
−
}
 
−
 
−
.top-content {
 
−
background-color: #fff;
 
−
padding: 3%;
 
−
margin: 3%
 
−
border-radius: 5px;
 
−
-webkit- border-radius: 5px;
 
−
-moz-border-radius: 5px;
 
−
}
 
−
.post-info {
 
−
font-size: 90%;
 
−
color: #999;
 
−
font-style: italic;
 
−
margin-top: -2%;
 
−
}
 
−
 
−
.post-content {
 
−
display: inline;
 
−
}
 
−
 
−
 
−
</style>
 
  
 +
-->
 +
    </div>
 +
    <div class="footer">
 +
    <div class="footer-wide">
 +
      <a href="http://www.auf.nl/" style="order:1" target="_blank">
 +
      <img alt="Auf" class="footer-sponsor-img" id="auf" src="https://static.igem.org/mediawiki/2017/5/5c/TAmsterdam_auf.png">
 +
      </img>
 +
      </a>
 +
      <a href="https://www.gss.uva.nl/" style="order:15" target="_blank">
 +
      <img alt="Gss" class="footer-sponsor-img" id="gss" src="https://static.igem.org/mediawiki/2017/d/d8/TAmsterdam_gss.png">
 +
      </img>
 +
      </a>
 +
      <a href="https://www.aimms.vu.nl/en/index.aspx" style="order:9" target="_blank">
 +
      <img alt="Aimms1" class="footer-sponsor-img" id="aimms" src="https://static.igem.org/mediawiki/2017/8/8d/TAmsterdam_aimms1.png">
 +
      </img>
 +
      </a>
 +
      <a href="https://www.amsterdamsciencepark.nl/" style="order:11" target="_blank">
 +
      <img alt="Sciencepark" class="footer-sponsor-img" id="sciencepark" src="https://static.igem.org/mediawiki/2017/b/b2/TAmsterdam_sciencepark.png">
 +
      </img>
 +
      </a>
 +
      <a href="https://www.dsm.com/" style="order:5" target="_blank">
 +
      <img alt="Dsm" class="footer-sponsor-img" id="dsm" src="https://static.igem.org/mediawiki/2017/3/38/TAmsterdam_dsm.png">
 +
      </img>
 +
      </a>
 +
      <a href="https://eu.idtdna.com/site" style="order:3" target="_blank">
 +
      <img alt="Idt" class="footer-sponsor-img" id="idt" src="https://static.igem.org/mediawiki/2017/c/c5/TAmsterdam_idt.png">
 +
      </img>
 +
      </a>
 +
      <a href="https://www.matrixic.nl/" style="order:12" target="_blank">
 +
      <img alt="Matrix" class="footer-sponsor-img" id="matrix" src="https://static.igem.org/mediawiki/2017/5/57/TAmsterdam_matrix.png">
 +
      </img>
 +
      </a>
 +
      <a href="http://www.merckmillipore.com/" style="order:10" target="_blank">
 +
      <img alt="Merck" class="footer-sponsor-img" id="merck" src="https://static.igem.org/mediawiki/2017/e/e5/TAmsterdam_merck.png">
 +
      </img>
 +
      </a>
 +
      <a href="http://rivm.nl/en" style="order:14" target="_blank">
 +
      <img alt="Rivm" class="footer-sponsor-img" id="rivm" src="https://static.igem.org/mediawiki/2017/f/f3/TAmsterdam_rivm.png">
 +
      </img>
 +
      </a>
 +
      <a href="http://phenomenex.com/" style="order:10" target="_blank">
 +
      <img alt="Phenomenex" class="footer-sponsor-img" id="phenomenex" src="https://static.igem.org/mediawiki/2017/0/02/TAmsterdam_phenomenex.png">
 +
      </img>
 +
      </a>
 +
      <a href="http://www.snapgene.com/" style="order:6" target="_blank">
 +
      <img alt="Snapgene" class="footer-sponsor-img" id="snapgene" src="https://static.igem.org/mediawiki/2017/8/80/TAmsterdam_snapgene.png">
 +
      </img>
 +
      </a>
 +
      <a href="http://sils.uva.nl/" style="order:8" target="_blank">
 +
      <img alt="Sils" class="footer-sponsor-img" id="sils" src="https://static.igem.org/mediawiki/2017/e/e4/TAmsterdam_sils.png"/>
 +
      </a>
 +
      <a href="https://www.unitedconsumers.com/" style="order:7" target="_blank">
 +
      <img alt="United consumers" class="footer-sponsor-img" id="unitedconsumers" src="https://static.igem.org/mediawiki/2017/2/2d/TAmsterdam_united_consumers.png"/>
 +
      </a>
 +
      <a href="https://www.vu.nl/en/" style="order:2" target="_blank">
 +
      <img alt="Vu" class="footer-sponsor-img" id="vu" src="https://static.igem.org/mediawiki/2017/5/5a/TAmsterdam_vu.png"/>
 +
      </a>
 +
      <a href="https://science.vu.nl/en/index.aspx" style="order:4" target="_blank">
 +
      <img alt="Vu fs" class="footer-sponsor-img" id="vufs" src="https://static.igem.org/mediawiki/2017/3/38/TAmsterdam_vu_fs.png"/>
 +
      </a>
 +
      <a href="https://www.cs.vu.nl/en/" style="order:13" target="_blank">
 +
      <img alt="Vu cs" class="footer-sponsor-img" id="vucs" src="https://static.igem.org/mediawiki/2017/c/ca/TAmsterdam_vu_cs.png"/>
 +
      </a>
 +
    </div>
 +
    <!--//
 +
        <div class="footer-col-right">
 +
          <a href="mailto:igem17.ams@gmail.com" target="_top">
 +
            <img src="/images/email_logo.jpg" class="footer-sponsor-img">
 +
          </a>
 +
          <a href="https://www.facebook.com/igem2017amsterdam/" target="_blank">
 +
            <img src="/images/facebook_logo.png" class="footer-sponsor-img">
 +
          </a>
 +
        </div>
 +
        //-->
 +
    </div>
 +
  </div>
 +
  </div>
 +
</div>
 
</html>
 
</html>
−
 
−
{{Amsterdam2}}
 

Latest revision as of 11:25, 23 November 2017

Home

Project

Project

Practices

Practices

Lab

Lab

Team

Team

Achievements

Achievements

We are running out of oil and the climate is changing drastically due to the emission of greenhouse gases such as CO 2 .

But what if CO 2 were a resource, rather than a wasteful pollutant; and might even replace oil?

Early attempts to achieve this have focused on producing sugars with plants, which are then subsequently fermented by bacteria to produce liquid fuels and other chemicals.

But there is a better way, where no arable land is needed and which is faster and more efficient. This is the way our team has chosen to go, using cyanobacteria to take up CO 2 and directly convert it into the valuable chemical FUMARATE

PROJECT

Fumarate is used to make certain plastics, food additives, and medicine and is currently made from petroleum. The global market size of fumarate is estimated to exceed $760 million by 2020!

We are 6 students from Amsterdam with a passion for creating a more sustainable future.

We joined iGEM because we did not just wanted to ‘do science’ but we wanted to reach out to society, learn about communicating our research, and work on a shared vision in a small dynamic team.

Guided by our fantastic supervisors, and owing to the generosity of our sponsors, we have managed to successfully conclude this challenging and incredibly rewarding project.

We want to especially thank the Molecular Microbial Physiology group at the University of Amsterdam who have welcomed us in their lab for the past 7 months.

Auf Gss Aimms1 Sciencepark Dsm Idt Matrix Merck Rivm Phenomenex Snapgene Sils United consumers Vu Vu fs Vu cs