Difference between revisions of "Team:BostonU HW/Transformation"

 
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color: red;
 
color: red;
 
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#page_background{
 
#page_background{
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background-image: <!-- insert image here -->;
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background-image: url("https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png");
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background-size:100%;
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#design_control,#design_flow,#mill_flow,#mill_control{
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<!-- *************THIS IS WHERE PAGE STARTS************* -->
 
<!-- *************THIS IS WHERE PAGE STARTS************* -->
 
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<div class="landing-page">
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<div id="page_background">
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<div class="wrapper" id="page_background">
 
<div class="header" id="Header_Pic">
 
<div class="header" id="Header_Pic">
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<img src="https://static.igem.org/mediawiki/2017/f/f9/MARS_Background1.png" alt="MARS" id="MARS">
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<img src="https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png" id="BACKGROUND">
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<div class="container" margin-top:"2%;">
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<div class="col-md-3">
 +
 
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<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" width="100%" style="margin-top:-37%;">
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</div>
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<div class="col-md-9" style="color:#eef1f5; font-size:100px; font-family:Arial,Gadget,sans-serif; margin-top:1%;">
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Transformation
 
</div>
 
</div>
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<div class="main main-raised" id = "main_text">
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</div>
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<div class="parent">
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</div>
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<div id="narrow">
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<div class="main main-raised" style="margin-top:2%;">
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<div class="section text-center section-landing">
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<div class="container">
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<div class="row">
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<div class="col-md-12">
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<!-- This is the main text -->
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<div class="text_section">
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<div>
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<h1>Summary</h1>
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<h2 class="title" id="Title">Cell Transformation</h2>
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<div class="text" style="margin-bottom:3%;">
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<h5 class="description" id="Description">Designed by Sarah N.</h5>
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Bacterial transformation is a commonly used protocol in synthetic biology. It can be used for a variety of functions, such as testing whether or not a genetic circuit is functional. Transformation allows bacterial cells, such as Escherichia coli, to take in and express external DNA fragments. Transformation consists of heat shock to damage cells and promote the taking up of external plasmids, recovery to prevent cells from dying, and a final culturing. From there, cells are analyzed.
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This is a chip that performs cellular transformation. There are three main regions.
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</div>
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First, is the metering section. Second, is a time dependent mixing section.
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</div>
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Lastly, is a recovery period. The control layer is also not very complicated. Etc.
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</div>
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<br>
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</div>
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</br>
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<div class="container">
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</div>
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<div class="col-md-9">
−
<!-- This is the main text -->
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<div class="text">
 +
This microfluidic chip is designed to perform transformation. Suspended cells and plasmid are metered on the chip and are then mixed together. The solution then undergoes heat shock in a time-dependent mixing element for exactly 30 seconds. The solution can then be pipetted out from the chip into a recovery tube on ice.
 +
                                <br><br>
 +
This chip has been milled and tested, but not deemed fully fluid functional as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
  
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<!-- This is the tabs -->
 
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<div class="profile-tabs">
 
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<div class="nav-align-center">
 
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<ul class="nav nav-pills nav-pills-danger" role="tablist">
 
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<li class="active">
 
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<a href="#flow" role="tab" data-toggle="tab">
 
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<i class="material-icons">camera</i>
 
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Design
 
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</a>
 
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</li>
 
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<li>
 
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<a href="#control" role="tab" data-toggle="tab">
 
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<i class="material-icons">memory</i>
 
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Milled
 
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</a>
 
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</li>
 
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</div>
 
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</div>
 
 
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<div class="col-md-3">
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<div class="tab-pane active" id="flow">
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<div style="text-align:center; margin:auto; vertical-align:middle;" >
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<div class="row">
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  <a href="https://2017.igem.org/Team:BostonU_HW/Demonstrate#Chips">
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<div id="Pics">
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    <button class="btn btn-primary btn-lg btn-danger" style="margin-top:0px;">MARS Repository<i class="material-icons">replay</i></button>
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<img src="https://static.igem.org/mediawiki/2017/d/df/TransFlow.png" class="img-rounded" id="design_flow" />
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  </a>
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<h5><u>Flow Layer</u></h5>
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</div>
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</div>
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<div style="text-align:center; margin:auto; vertical-align:middle;" >
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</div>
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<a href="https://static.igem.org/mediawiki/2017/6/66/MARSTransformation.zip"download>
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<div class="row">
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<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
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<div id="Pics">
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</a>
−
<img src="https://static.igem.org/mediawiki/2017/9/94/TransControl.png" class="img-rounded" id="design_control" />
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</div>
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<h5><u>Control Layer</u></h5>
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<div class="profile-tabs">
−
</div>
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<div class="nav-align-center">
−
</div>
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<ul class="nav nav-pills nav-pills-danger" role="tablist">
−
</div>
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<li class="active">
−
<div class="tab-pane text-center" id="control">
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<a href="#Design" role="tab" data-toggle="tab">
−
<div class="row">
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<i class="material-icons">view_agenda</i>
−
<div id="Pics">
+
Design
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<img src="chips/mill.jpg" class="img-rounded" id="mill_flow"/>
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</a>
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<h5><u>Flow Layer</u></h5>
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</li>
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<img src="" class="img-rounded" />
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<li>
−
</div>
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<a href="#Mill" role="tab" data-toggle="tab">
−
<div id="Pics">
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<i class="material-icons">device_hub</i>
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<img src="chips/mill.jpg" class="img-rounded" id="mill_flow"/>
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Milled
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<h5><u>Flow Layer</u></h5>
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</a>
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<img src="" class="img-rounded" />
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</li>
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</div>
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</ul>
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</div>
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</div>
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<div class="row" style="background">
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</div>
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<div class="section text-center section-landing">
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</div>
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<div class="row">
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<h2 class = "title">Experiment</h2>
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<div class="container">
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<h5 class = "description">Brief Description</h5>
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<div class="tab-content gallery text-center">
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<iframe width="600" height="315" src="https://www.youtube.com/embed/H2e8ujMEEOY" frameborder="0" allowfullscreen></iframe>
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<div class="tab-pane active" id="Design">
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<br>
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<div class="col-md-6 text-center">
−
<b><a href= "https://github.com/CIDARLAB/MARS">Download Files to Build your Own!</a><b>
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<img class="pics" src="https://static.igem.org/mediawiki/2017/8/83/MARS_Transformation_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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</br>
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<div class="row text-center">
−
</div>
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<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
 
</div>
 
</div>
−
</div>
+
</div>
 +
<div class="col-md-6 text-center">
 +
<img class="pics" src="https://static.igem.org/mediawiki/2017/c/c8/MARS_Transformation_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
 +
<div class="row text-center">
 +
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="tab-pane" id="Mill">
 +
<div class="col-md-6 text-center">
 +
<img class="pics" src="https://static.igem.org/mediawiki/2017/4/41/MARS_Transformation_MF.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
 +
<div class="row">
 +
<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
 +
</div>
 +
</div>
 +
<div class="col-md-6 text-center">
 +
<img class="pics" src="https://static.igem.org/mediawiki/2017/7/76/MARS_Transformation_MC.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
 +
<div class="row text-center">
 +
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
  
 +
<div class="container">
 +
<div class="col-md-6" style="margin-bottom:5%;">
 +
<video style="width:100%; margin-top:20px; padding-top:18px;" controls>
 +
  <source src="https://static.igem.org/mediawiki/2017/d/d0/MARS_TransformationVid.mp4" type="video/mp4">
 +
</video>
 +
</div>
 +
<div class="col-md-6">
 +
<h1>Testing</h1>
 +
<div class="text">
 +
This following video shows a test of the chip using colored water. This is to show the functionality of the chip.
 +
No biological material was inserted into this chip.
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
  
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</div>
+
<!-- THIS IS FOOTER -->
 +
<div class="wrapper" style="background:#1c1f1f; margin-top:0px;margin-right:0px !important; margin-left:0px !important;" id="Footer">
 +
<div class="container" style="text-align:center !important">
 +
 
 +
<div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
 +
<h3>CONTACT US</h3>
 +
<div style="text-align:center;">
 +
<a href="mailto:igembuhw@gmail.com">
 +
<img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
 +
</a>
 +
<a href="https://www.instagram.com/buigemhardware/?hl=en">
 +
<img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
 +
</a>
 +
<a href="https://twitter.com/igemhwbu">
 +
<img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
 +
</a>
 +
</div>
 +
</div>
 +
<div class="col-md-10" style="margin-bottom:30px;">
 +
<img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
 +
</div>
 
</div>
 
</div>
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</div>
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</div>
 
</div>
 
</body>
 
</body>

Latest revision as of 16:25, 30 November 2017

BostonU_HW

Transformation
Transformation

Summary

Bacterial transformation is a commonly used protocol in synthetic biology. It can be used for a variety of functions, such as testing whether or not a genetic circuit is functional. Transformation allows bacterial cells, such as Escherichia coli, to take in and express external DNA fragments. Transformation consists of heat shock to damage cells and promote the taking up of external plasmids, recovery to prevent cells from dying, and a final culturing. From there, cells are analyzed.
This microfluidic chip is designed to perform transformation. Suspended cells and plasmid are metered on the chip and are then mixed together. The solution then undergoes heat shock in a time-dependent mixing element for exactly 30 seconds. The solution can then be pipetted out from the chip into a recovery tube on ice.

This chip has been milled and tested, but not deemed fully fluid functional as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
Picture
Picture
Picture
Picture

Testing

This following video shows a test of the chip using colored water. This is to show the functionality of the chip. No biological material was inserted into this chip.