Difference between revisions of "Team:BostonU HW/Transformation"

 
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<title>Digestion</title>
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<title>Transformation</title>
  
 
<meta content='width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0' name='viewport' />
 
<meta content='width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0' name='viewport' />
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#page_background{
 
#page_background{
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background-image: url("https://static.igem.org/mediawiki/2017/0/04/MARS_General_Background.png");
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background-image: url("https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png");
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background-size:100%;
 
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#Header_Pic{
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#TITLE{
 
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width: 70%;
 
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<div class="wrapper" id="page_background">
 
<div class="wrapper" id="page_background">
 
<div class="header" id="Header_Pic">
 
<div class="header" id="Header_Pic">
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
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<img src="https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png" id="BACKGROUND">
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<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
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<div class="container" margin-top:"2%;">
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<img src="https://static.igem.org/mediawiki/2017/7/7a/MARS_Digestion.png" id="TITLE">
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<div class="col-md-3">
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<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" width="100%" style="margin-top:-37%;">
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</div>
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<div class="col-md-9" style="color:#eef1f5; font-size:100px; font-family:Arial,Gadget,sans-serif; margin-top:1%;">
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Transformation
 
</div>
 
</div>
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<div class="main main-raised" style="margin-bottom:5%;">
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</div>
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</div>
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<div class="main main-raised" style="margin-top:2%;">
 
<div class="container">
 
<div class="container">
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<div class="col-md-9">
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<div class="col-md-12">
 
<div class="text_section">
 
<div class="text_section">
 
<h1>Summary</h1>
 
<h1>Summary</h1>
 
<div class="text" style="margin-bottom:3%;">
 
<div class="text" style="margin-bottom:3%;">
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DNA digestion, or DNA fragmentation, is a basic protocol in synthetic biology. This is typically performed prior to analysis of the DNA sequence, or in order to perform further protocols. Restriction enzymes are mixed and then incubated with DNA in a buffer solution. This yields DNA fragments cleaved at specific sites according to the enzymes used.
+
Bacterial transformation is a commonly used protocol in synthetic biology. It can be used for a variety of functions, such as testing whether or not a genetic circuit is functional. Transformation allows bacterial cells, such as Escherichia coli, to take in and express external DNA fragments. Transformation consists of heat shock to damage cells and promote the taking up of external plasmids, recovery to prevent cells from dying, and a final culturing. From there, cells are analyzed.
 
</div>
 
</div>
 
</div>
 
</div>
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</div>
 
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<div class="col-md-3" style="text-align:center; margin:auto; vertical-align:middle;" >
 
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<a href="MARStest.zip"download>
 
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<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
 
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</a>
 
 
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</div>
 
</div>
 
</div>
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<div class="col-md-9">
 
<div class="col-md-9">
 
<div class="text">
 
<div class="text">
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This microfluidic chip design performs DNA digestion. The desired DNA segment, restriction enzymes, water and buffer solution are inputted and mixed. The resulting solution is then sealed in an incubation chamber on top of which a heating element is placed. After the designated incubation time has passed, the liquid can then be transferred from the chip using a pipett
+
This microfluidic chip is designed to perform transformation. Suspended cells and plasmid are metered on the chip and are then mixed together. The solution then undergoes heat shock in a time-dependent mixing element for exactly 30 seconds. The solution can then be pipetted out from the chip into a recovery tube on ice.
 +
                                <br><br>
 +
This chip has been milled and tested, but not deemed fully fluid functional as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
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</div>
 
</div>
 
</div>
 
</div>
 
<div class="col-md-3">
 
<div class="col-md-3">
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<div style="text-align:center; margin:auto; vertical-align:middle;" >
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  <a href="https://2017.igem.org/Team:BostonU_HW/Demonstrate#Chips">
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    <button class="btn btn-primary btn-lg btn-danger" style="margin-top:0px;">MARS Repository<i class="material-icons">replay</i></button>
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  </a>
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</div>
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<div style="text-align:center; margin:auto; vertical-align:middle;" >
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<a href="https://static.igem.org/mediawiki/2017/6/66/MARSTransformation.zip"download>
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<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
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</a>
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</div>
 
<div class="profile-tabs">
 
<div class="profile-tabs">
 
<div class="nav-align-center">
 
<div class="nav-align-center">
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<div class="tab-pane active" id="Design">
 
<div class="tab-pane active" id="Design">
 
<div class="col-md-6 text-center">
 
<div class="col-md-6 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/2/28/MARS_Digestion_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/8/83/MARS_Transformation_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<div class="row text-center" style="margin-bottom:5%;">
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<div class="row text-center">
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<button class="btn btn-info btn-round" ><span style="font-size:17px;">Flow Layer</span></button>
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<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
 
</div>
 
</div>
 
</div>
 
</div>
 
<div class="col-md-6 text-center">
 
<div class="col-md-6 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/f/fa/MARS_Digestion_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/c/c8/MARS_Transformation_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<div class="row text-center" style="margin-bottom:5%;">
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<div class="row text-center">
 
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
 
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
 
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</div>
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<div class="tab-pane" id="Mill">
 
<div class="tab-pane" id="Mill">
 
<div class="col-md-6 text-center">
 
<div class="col-md-6 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/6/6b/MARS_Digestion_MF.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/4/41/MARS_Transformation_MF.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
 
<div class="row">
 
<div class="row">
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<button class="btn btn-info btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Flow Layer</span></button>
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<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
 
</div>
 
</div>
 
</div>
 
</div>
 
<div class="col-md-6 text-center">
 
<div class="col-md-6 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/2/25/MARS_Digestion_MC.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/7/76/MARS_Transformation_MC.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
 
<div class="row text-center">
 
<div class="row text-center">
−
<button class="btn btn-danger btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Control Layer</span></button>
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<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
 
</div>
 
</div>
 
</div>
 
</div>
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</div>
 
</div>
 
</div>
 
</div>
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<div class="container">
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<div class="col-md-6" style="margin-bottom:5%;">
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<video style="width:100%; margin-top:20px; padding-top:18px;" controls>
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  <source src="https://static.igem.org/mediawiki/2017/d/d0/MARS_TransformationVid.mp4" type="video/mp4">
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</video>
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</div>
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<div class="col-md-6">
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<h1>Testing</h1>
 +
<div class="text">
 +
This following video shows a test of the chip using colored water. This is to show the functionality of the chip.
 +
No biological material was inserted into this chip.
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</div>
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</div>
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</div>
 
</div>
 
</div>
 
</div>
 
</div>
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<!-- THIS IS FOOTER -->
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<div class="wrapper" style="background:#1c1f1f; margin-top:0px;margin-right:0px !important; margin-left:0px !important;" id="Footer">
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<div class="container" style="text-align:center !important">
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<div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
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<h3>CONTACT US</h3>
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<div style="text-align:center;">
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<a href="mailto:igembuhw@gmail.com">
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<img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
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</a>
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<a href="https://www.instagram.com/buigemhardware/?hl=en">
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<img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
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</a>
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<a href="https://twitter.com/igemhwbu">
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<img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
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</a>
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</div>
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</div>
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<div class="col-md-10" style="margin-bottom:30px;">
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<img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
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</div>
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</div>
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</div>
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</div>
 
</div>
 
</body>
 
</body>

Latest revision as of 16:25, 30 November 2017

BostonU_HW

Transformation
Transformation

Summary

Bacterial transformation is a commonly used protocol in synthetic biology. It can be used for a variety of functions, such as testing whether or not a genetic circuit is functional. Transformation allows bacterial cells, such as Escherichia coli, to take in and express external DNA fragments. Transformation consists of heat shock to damage cells and promote the taking up of external plasmids, recovery to prevent cells from dying, and a final culturing. From there, cells are analyzed.
This microfluidic chip is designed to perform transformation. Suspended cells and plasmid are metered on the chip and are then mixed together. The solution then undergoes heat shock in a time-dependent mixing element for exactly 30 seconds. The solution can then be pipetted out from the chip into a recovery tube on ice.

This chip has been milled and tested, but not deemed fully fluid functional as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
Picture
Picture
Picture
Picture

Testing

This following video shows a test of the chip using colored water. This is to show the functionality of the chip. No biological material was inserted into this chip.