Difference between revisions of "Team:BostonU HW/Isolation"

 
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<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1" />
 
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<title>Cell Lysis</title>
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<title>Cell Sorting</title>
  
 
<meta content='width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0' name='viewport' />
 
<meta content='width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0' name='viewport' />
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#page_background{
 
#page_background{
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background-image: url("https://static.igem.org/mediawiki/2017/0/04/MARS_General_Background.png");
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        background-image: url("https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png");
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        background-size:100% ;
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    }
 
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<div class="wrapper" id="page_background">
 
<div class="wrapper" id="page_background">
 
<div class="header" id="Header_Pic">
 
<div class="header" id="Header_Pic">
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
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<img src="https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png" id="BACKGROUND">
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<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
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<div class="container" margin-top:"2%;">
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<img src="https://static.igem.org/mediawiki/2017/3/33/MARS_Cell_Lysis.png" id="TITLE">
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<div class="col-md-3">
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<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" width="100%" style="margin-top:-37%;">
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</div>
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<div class="col-md-9" style="color:#eef1f5; font-size:100px; font-family:Arial,Gadget,sans-serif; margin-top:1%;">
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Cell Sorting
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</div>
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</div>
 
</div>
 
</div>
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<div class="main main-raised" style="margin-bottom:5%;">
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<div class="main main-raised" style="margin-top:2%;">
 
<div class="container">
 
<div class="container">
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<div class="col-md-9">
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<div class="col-md-12">
 
<div class="text_section">
 
<div class="text_section">
 
<h1>Summary</h1>
 
<h1>Summary</h1>
 
<div class="text" style="margin-bottom:3%;">
 
<div class="text" style="margin-bottom:3%;">
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Cell lysis is a commonly used protocol in synthetic biology. It can be performed through a variety of different methods, however we had focused on chemical cellular lysis. Cell Lysis is used to extract and isolate DNA from a specific type of cell. This is an extremely important step in building genetic circuits in order to utilize specific coding regions in a cell's DNA. Chemical cell lysis involves introducing cells to a series of buffers in order to degrade the cell’s outer membrane and collect the DNA that is released.
+
The ability to sort cells by type or physical properties is a valuable tool in many synthetic biology labs. Prior to analysis or in order to perform specialized protocols, creating homogenous cell suspensions from a mixture is necessary. In addition to sorting cells, the removal of cell fragments, activated magnetic particles or unwanted debris through sorting also makes up a key part of purification protocols.
 
</div>
 
</div>
 
</div>
 
</div>
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</div>
 
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<div class="col-md-3" style="text-align:center; margin:auto; vertical-align:middle;" >
 
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<a href="MARStest.zip"download>
 
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<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
 
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</a>
 
 
</div>
 
</div>
 
</div>
 
</div>
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<div class="col-md-9">
 
<div class="col-md-9">
 
<div class="text">
 
<div class="text">
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This microfluidic chip is designed to perform chemical cell lysis. Suspended cells and an lysing buffer would be mixed inside the cell. This mixture of buffer and cells would be then mixed with a neutralization buffer to prevent DNA degradation. The mixture would then move to the diamond chamber where the DNA binds magnetic particles. Lastly, an elution buffer would be input and to clean and release the DNA from the magnetic particles.
+
This microfluidic chip design carries out cell sorting as a cell suspension is passed through it. Cells are sorted based on size and pushed to the periphery of the channel. These cells are then carried away from the main solution through the two periphery outputs, and the cell-free solution can be collected from the central output.
 +
                              <br><br>
 +
This chip has been milled, tested and deemed fully fluid functional. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
 +
<br><br>
 +
The design for this chip was inspired by the following paper:
 +
<br>
 +
Hou, H. W., Bhagat, A. A. S., Lin Chong, A. G., Mao, P., Wei Tan, K. S., Han, J., & Lim, C. T. (2010). Deformability based cell margination—A simple microfluidic design for malaria-infected erythrocyte separation. Lab on a Chip, 10(19), 2605. <a href = "https://doi.org/10.1039/c003873c">https://doi.org/10.1039/c003873c</a>
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</div>
 
</div>
 
</div>
 
</div>
 
<div class="col-md-3">
 
<div class="col-md-3">
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<div style="text-align:center; margin:auto; vertical-align:middle;" >
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  <a href="https://2017.igem.org/Team:BostonU_HW/Demonstrate#Chips">
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    <button class="btn btn-primary btn-lg btn-danger" style="margin-top:0px;">MARS Repository<i class="material-icons">replay</i></button>
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  </a>
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</div>
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<div style="text-align:center; margin:auto; vertical-align:middle;" >
 +
<a href="https://static.igem.org/mediawiki/2017/4/45/MARSSort.zip"download>
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<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
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</a>
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</div>
 
<div class="profile-tabs">
 
<div class="profile-tabs">
 
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<div class="tab-content gallery text-center">
 
<div class="tab-content gallery text-center">
 
<div class="tab-pane active" id="Design">
 
<div class="tab-pane active" id="Design">
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<div class="col-md-6 text-center">
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<div class="col-md-12 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/b/bb/MARS_Lysis_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/6/69/MARS_CellSorting_F.png" alt="Picture" style="margin-top:20px; padding-top:18px; width:45% !important;">
 
<div class="row text-center">
 
<div class="row text-center">
 
<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
 
<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
 
</div>
 
</div>
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</div>
 
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<div class="col-md-6 text-center">
 
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<img class="pics" src="https://static.igem.org/mediawiki/2017/0/00/MARS_Lysis_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
 
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<div class="row text-center">
 
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<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
 
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</div>
 
 
</div>
 
</div>
 
</div>
 
</div>
 
<div class="tab-pane" id="Mill">
 
<div class="tab-pane" id="Mill">
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<div class="col-md-6 text-center">
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<div class="col-md-12 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/0/08/MARS_Lysis_M.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/thumb/7/7f/MARS_CellSorting_MF.png/800px-MARS_CellSorting_MF.png.jpeg" alt="Picture" style="margin-top:20px; padding-top:18px; width:45% !important;">
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<div class="row">
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<div class="row text-center">
 
<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
 
<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
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</div>
 
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</div>
 
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<div class="col-md-6 text-center">
 
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<img class="pics" src="https://static.igem.org/mediawiki/2017/0/00/MARS_Lysis_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
 
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<div class="row text-center">
 
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<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
 
 
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<div class="col-md-6" style="margin-bottom:5%;">
 
<div class="col-md-6" style="margin-bottom:5%;">
 
<video style="width:100%; margin-top:20px; padding-top:18px;" controls>
 
<video style="width:100%; margin-top:20px; padding-top:18px;" controls>
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   <source src="https://static.igem.org/mediawiki/2017/4/47/MARS_LysisVid.mp4" type="video/mp4">
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   <source src="https://static.igem.org/mediawiki/2017/7/75/MARS_CellSortingVid.mp4" type="video/mp4">
 
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<h1>Testing</h1>
 
<h1>Testing</h1>
 
<div class="text">
 
<div class="text">
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This following video shows a test of the chip using colored water. This is to show help explain the functionality of the chip.
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This following video shows a test of the chip using colored water. This is to show the functionality of the chip.
 
No biological material was inserted into this chip.
 
No biological material was inserted into this chip.
 
</div>
 
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</div>
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<!-- THIS IS FOOTER -->
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<div class="wrapper" style="background:#1c1f1f; margin-top:0px;margin-right:0px !important; margin-left:0px !important;" id="Footer">
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<div class="container" style="text-align:center !important">
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<div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
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<h3>CONTACT US</h3>
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<div style="text-align:center;">
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<a href="mailto:igembuhw@gmail.com">
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<img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
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</a>
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<a href="https://www.instagram.com/buigemhardware/?hl=en">
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<img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
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</a>
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<a href="https://twitter.com/igemhwbu">
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<img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
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</a>
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</div>
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</div>
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<div class="col-md-10" style="margin-bottom:30px;">
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<img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
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</div>
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</div>
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</div>
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</body>
 
</body>

Latest revision as of 16:34, 30 November 2017

BostonU_HW

Cell Sorting
Cell Sorting

Summary

The ability to sort cells by type or physical properties is a valuable tool in many synthetic biology labs. Prior to analysis or in order to perform specialized protocols, creating homogenous cell suspensions from a mixture is necessary. In addition to sorting cells, the removal of cell fragments, activated magnetic particles or unwanted debris through sorting also makes up a key part of purification protocols.
This microfluidic chip design carries out cell sorting as a cell suspension is passed through it. Cells are sorted based on size and pushed to the periphery of the channel. These cells are then carried away from the main solution through the two periphery outputs, and the cell-free solution can be collected from the central output.

This chip has been milled, tested and deemed fully fluid functional. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.

The design for this chip was inspired by the following paper:
Hou, H. W., Bhagat, A. A. S., Lin Chong, A. G., Mao, P., Wei Tan, K. S., Han, J., & Lim, C. T. (2010). Deformability based cell margination—A simple microfluidic design for malaria-infected erythrocyte separation. Lab on a Chip, 10(19), 2605. https://doi.org/10.1039/c003873c
Picture
Picture

Testing

This following video shows a test of the chip using colored water. This is to show the functionality of the chip. No biological material was inserted into this chip.