Difference between revisions of "Team:BostonU HW/Antibiotic"

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<title>Cell Sorting</title>
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<title>Antibiotice Resistance</title>
  
 
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
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<div class="col-md-9" style="color:#eef1f5; font-size:80px; font-family:Arial,Gadget,sans-serif; margin-top:-2.5%;">
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Antibiotic<br><br><br><br>Resistance
 
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<h1>Summary</h1>
 
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The ability to sort cells by type or physical properties is a valuable tool in many synthetic biology labs. Prior to analysis or in order to perform specialised protocols, creating homogenous cell suspensions from a mixture is necessary. In addition to sorting cells, the removal of cell fragments, activated magnetic particles or unwanted debris through sorting also makes up a key part of purification protocols.
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Antibiotic resistance is an important part of evaluating the success of a synthetic biology experiment. This test is usually performed after transforming your plasmid into your chassis organism. The resistance is due to selection markers that are placed within the plasmid that contains your biological part(s). The cells that were not transformed properly will die to their lack of resistance. The remaining cells can be utilized to perform subsequent experiments.
 
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<a href="MARStest.zip"download>
 
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This microfluidic chip design carries out cell sorting as a cell suspension is passed through it. Cells are sorted based on size and pushed to the periphery of the channel. These cells are then carried away from the main solution through the two periphery outputs, and the cell-free solution can be collected from the central output.
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Antibiotic resistance is an important part of evaluating the success of a synthetic biology experiment.  This test is usually performed after transforming your plasmid into your chassis organism. The resistance is due to selection markers that are placed within the plasmid that contains your biological part(s). The cells that were not transformed properly will die to their lack of resistance. The remaining cells can be utilized to perform subsequent experiments.
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                                <br><br>
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This chip has not been tested as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
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The design for this chip was inspired by the following paper:
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<br>
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Mohan, R., Mukherjee, A., Sevgen, S. E., Sanpitakseree, C., Lee, J., Schroeder, C. M., & Kenis, P. J. A. (2013). A multiplexed microfluidic platform for rapid antibiotic susceptibility testing. Biosensors and Bioelectronics, 49, 118–125. <a href="https://doi.org/10.1016/j.bios.2013.04.046">https://doi.org/10.1016/j.bios.2013.04.046</a>
 
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  <a href="https://2017.igem.org/Team:BostonU_HW/Demonstrate#Chips">
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    <button class="btn btn-primary btn-lg btn-danger" style="margin-top:0px;">MARS Repository<i class="material-icons">replay</i></button>
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  </a>
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</div>
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<div style="text-align:center; margin:auto; vertical-align:middle;" >
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<a href="https://static.igem.org/mediawiki/2017/e/ee/MARS_Antibiotic_Resistance.zip"download>
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<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
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<img class="pics" src="https://static.igem.org/mediawiki/2017/5/5b/MARS_Antibiotic_F.png" alt="Picture" style="margin-top:20px; padding-top:18px; width:45% !important;">
 
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<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
 
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<img class="pics" src="https://static.igem.org/mediawiki/2017/6/6c/MARS_Antibiotic_MF.png" alt="Picture" style="margin-top:20px; padding-top:18px; width:45% !important;">
 
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    <div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
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      <h3>CONTACT US</h3>
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    <div style="text-align:center;">
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      <a href="mailto:igembuhw@gmail.com">
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      <img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
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      </a>
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        <a href="https://www.instagram.com/buigemhardware/?hl=en">
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        <img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
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      </a>
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          <a href="https://twitter.com/igemhwbu">
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          <img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
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          </a>
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      </div>
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      </div>
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      <div class="col-md-10" style="margin-bottom:30px;">
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        <img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
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    </div>
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Latest revision as of 16:40, 30 November 2017

BostonU_HW

Antibiotice Resistance
Antibiotic



Resistance

Summary

Antibiotic resistance is an important part of evaluating the success of a synthetic biology experiment. This test is usually performed after transforming your plasmid into your chassis organism. The resistance is due to selection markers that are placed within the plasmid that contains your biological part(s). The cells that were not transformed properly will die to their lack of resistance. The remaining cells can be utilized to perform subsequent experiments.
Antibiotic resistance is an important part of evaluating the success of a synthetic biology experiment. This test is usually performed after transforming your plasmid into your chassis organism. The resistance is due to selection markers that are placed within the plasmid that contains your biological part(s). The cells that were not transformed properly will die to their lack of resistance. The remaining cells can be utilized to perform subsequent experiments.

This chip has not been tested as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.

The design for this chip was inspired by the following paper:
Mohan, R., Mukherjee, A., Sevgen, S. E., Sanpitakseree, C., Lee, J., Schroeder, C. M., & Kenis, P. J. A. (2013). A multiplexed microfluidic platform for rapid antibiotic susceptibility testing. Biosensors and Bioelectronics, 49, 118–125. https://doi.org/10.1016/j.bios.2013.04.046
Picture
Picture