Difference between revisions of "Team:BostonU HW/Culturing"

(Created page with "{{Global_Menubar}} {{Team:BostonU_Hardware/CSS}} <html lang="en"> <head> <meta charset="utf-8" /> <link rel="apple-touch-icon" sizes="76x76" href="assets/img/apple-icon.png"...")
 
 
(12 intermediate revisions by 2 users not shown)
Line 8: Line 8:
 
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1" />
 
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1" />
  
−
<title>Cell Lysis</title>
+
<title>PCR</title>
  
 
<meta content='width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0' name='viewport' />
 
<meta content='width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0' name='viewport' />
Line 18: Line 18:
 
}
 
}
 
#page_background{
 
#page_background{
−
background-image: url("https://static.igem.org/mediawiki/2017/0/04/MARS_General_Background.png");
+
background-image: url("https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png");
 +
background-size:100% ;
 
}
 
}
 
#Header_Pic{
 
#Header_Pic{
Line 35: Line 36:
 
#TITLE{
 
#TITLE{
 
position: absolute;
 
position: absolute;
−
width: 40%;
+
width: 20%;
 
margin-left: 22%;
 
margin-left: 22%;
 
margin-top: 11%;
 
margin-top: 11%;
Line 65: Line 66:
 
<div class="wrapper" id="page_background">
 
<div class="wrapper" id="page_background">
 
<div class="header" id="Header_Pic">
 
<div class="header" id="Header_Pic">
−
<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
+
<img src="https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png" id="BACKGROUND">
−
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
+
<div class="container" margin-top:"2%;">
−
<img src="https://static.igem.org/mediawiki/2017/3/33/MARS_Cell_Lysis.png" id="TITLE">
+
<div class="col-md-3">
 +
 
 +
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" width="100%" style="margin-top:-37%;">
 +
</div>
 +
<div class="col-md-9" style="color:#eef1f5; font-size:100px; font-family:Arial,Gadget,sans-serif; margin-top:1%;">
 +
Cell Culturing
 +
</div>
 +
</div>
 
</div>
 
</div>
−
<div class="main main-raised" style="margin-bottom:5%;">
+
<div class="main main-raised" style="margin-top:2%;">
 
<div class="container">
 
<div class="container">
−
<div class="col-md-7">
+
<div class="col-md-9">
 
<div class="text_section">
 
<div class="text_section">
 
<h1>Summary</h1>
 
<h1>Summary</h1>
−
<div class="text">
+
<div class="text" style="margin-bottom:3%;">
−
Cell lysis is a commonly used protocol in synthetic biology. It can be performed through a variety of different methods, however we had focused on chemical cellular lysis. Cell Lysis is used to extract and isolate DNA from a specific type of cell. This is an extremely important step in building genetic circuits in order to utilize specific coding regions in a cell's DNA. Chemical cell lysis involves introducing cells to a series of buffers in order to degrade the cell’s outer membrane and collect the DNA that is released.
+
The SVG design file that is part of MARS repository section is a preliminary design for a complete microfluidic chip. The circulation cell culturing chamber portion of the chip was developed as part of BostonU HW's collaboration with the Harvard iGEM team. For more information regarding the development of this microfluidic chip visit the collaborations section of our wiki here:
 +
<br><br><a href="https://2017.igem.org/Team:BostonU_HW/Collaborations">
 +
<button class="btn btn-round btn-danger" style="margin:0% !important;">Collaborations</button>
 +
</a>
 
<br>
 
<br>
 
<br>
 
<br>
−
This microfluidic chip is designed to perform chemical cell lysis. Suspended cells and an lysing buffer would be mixed inside the cell. This mixture of buffer and cells would be then mixed with a neutralization buffer to prevent DNA degradation. The mixture would then move to the diamond chamber where the DNA binds magnetic particles. Lastly, an elution buffer would be input and to clean and release the DNA from the magnetic particles.
+
If you are interested in milling out this chip download the files by clicking on the download button!
 +
<br>
 +
<br>
 +
The design for this chip was inspired by the following paper:
 +
<br>
 +
Lee KS, Boccazzi P, Sinskey AJ, Ram RJ. Microfluidic chemostat and turbidostat with flow rate, oxygen, and temperature control for dynamic continuous culture. Lab Chip. 2011;11(10):1730-9. <a href="https://doi.org/10.1039/c1lc20019d">https://doi.org/10.1039/c1lc20019d</a>
 
</div>
 
</div>
 
</div>
 
</div>
−
<div style="text-align:center; margin:auto;" >
+
</div>
−
<a href="MARStest.zip"download>
+
<div style="text-align:center; margin:auto;  margin-top:140px; vertical-align:middle;" >
 +
  <a href="https://2017.igem.org/Team:BostonU_HW/Demonstrate#Chips">
 +
    <button class="btn btn-primary btn-lg btn-danger" style="margin-top:0px;">MARS Repository<i class="material-icons">replay</i></button>
 +
  </a>
 +
</div>
 +
<div class="col-md-3" style="text-align:center; margin:auto; vertical-align:middle;">
 +
<a href="https://static.igem.org/mediawiki/2017/2/26/MARS_HARVARD_CC.zip"download>
 
<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
 
<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
 
</a>
 
</a>
 
</div>
 
</div>
−
<div class="profile-tabs">
+
</div>
−
<div class="nav-align-center">
+
</div>
−
<ul class="nav nav-pills nav-pills-danger" role="tablist">
+
</div>
−
<li class="active">
+
−
<a href="#Design" role="tab" data-toggle="tab">
+
−
<i class="material-icons">view_agenda</i>
+
−
Design
+
−
</a>
+
−
</li>
+
−
<li>
+
−
<a href="#Mill" role="tab" data-toggle="tab">
+
−
<i class="material-icons">device_hub</i>
+
−
Milled
+
−
</a>
+
−
</li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
</div>
+
  
−
<div class="col-md-5">
+
<!-- THIS IS FOOTER -->
−
<div class="tab-content gallery text-center">
+
<div class="wrapper" style="background:#1c1f1f; margin-top:0px;margin-right:0px !important; margin-left:0px !important;" id="Footer">
−
<div class="tab-pane active" id="Design">
+
<div class="container" style="text-align:center !important">
−
<div class="row text-center">
+
−
<img class="pics" src="https://static.igem.org/mediawiki/2017/b/bb/MARS_Lysis_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
+
−
</div>
+
−
<div class="row text-center">
+
−
<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
+
−
</div>
+
−
<div class="row">
+
−
<img class="pics" src="https://static.igem.org/mediawiki/2017/0/00/MARS_Lysis_C.png" alt="Picture">
+
−
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
+
−
</div>
+
−
</div>
+
−
<div class="tab-pane" id="Mill">
+
−
<div class="row">
+
−
<img class="pics" src="https://static.igem.org/mediawiki/2017/0/08/MARS_Lysis_M.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
+
−
</div>
+
−
<div class="row">
+
−
<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
+
−
</div>
+
−
<div class="row">
+
−
<img class="pics" src="https://static.igem.org/mediawiki/2017/0/00/MARS_Lysis_C.png" alt="Picture">
+
−
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
</div>
+
  
−
</div>
+
<div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
−
 
+
<h3>CONTACT US</h3>
−
<div class="container">
+
<div style="text-align:center;">
−
<div class="col-md-6" style="margin-bottom:5%;">
+
<a href="mailto:igembuhw@gmail.com">
−
<video style="width:100%; margin-top:20px; padding-top:18px;" controls>
+
<img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
−
  <source src="https://static.igem.org/mediawiki/2017/4/47/MARS_LysisVid.mp4" type="video/mp4">
+
</a>
−
</video>
+
<a href="https://www.instagram.com/buigemhardware/?hl=en">
−
</div>
+
<img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
−
<div class="col-md-6">
+
</a>
−
<h1>Testing</h1>
+
<a href="https://twitter.com/igemhwbu">
−
<div class="text">
+
<img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
−
This following video shows a test of the chip using colored water. This is to show help explain the functionality of the chip.
+
</a>
−
No biological material was inserted into this chip.
+
 
</div>
 
</div>
 
</div>
 
</div>
 +
<div class="col-md-10" style="margin-bottom:30px;">
 +
<img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
 
</div>
 
</div>
−
</div>
 
 
</div>
 
</div>
 +
 +
</div>
 +
 +
 
</div>
 
</div>
 
</body>
 
</body>

Latest revision as of 16:41, 30 November 2017

BostonU_HW

PCR
Cell Culturing

Summary

The SVG design file that is part of MARS repository section is a preliminary design for a complete microfluidic chip. The circulation cell culturing chamber portion of the chip was developed as part of BostonU HW's collaboration with the Harvard iGEM team. For more information regarding the development of this microfluidic chip visit the collaborations section of our wiki here:



If you are interested in milling out this chip download the files by clicking on the download button!

The design for this chip was inspired by the following paper:
Lee KS, Boccazzi P, Sinskey AJ, Ram RJ. Microfluidic chemostat and turbidostat with flow rate, oxygen, and temperature control for dynamic continuous culture. Lab Chip. 2011;11(10):1730-9. https://doi.org/10.1039/c1lc20019d