Difference between revisions of "Team:Stuttgart/InterLab"

 
(39 intermediate revisions by 4 users not shown)
Line 1: Line 1:
 +
{{Template:Home}}
 
<html>
 
<html>
−
 
−
<!--- THIS IS WHERE THE HTML BEGINS --->
 
−
 
−
<head>
 
−
    <link href="https://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet"
 
−
          id="bootstrap-css">
 
−
    <style>
 
−
 
−
 
−
        /***************************************************** DEFAULT WIKI SETTINGS  ****************************************************/
 
−
 
−
        /* Clear the default wiki settings */
 
−
 
−
        #home_logo, #sideMenu {
 
−
            display: none;
 
−
        }
 
−
 
−
        #sideMenu, #top_title, .patrollink {
 
−
            display: none;
 
−
        }
 
−
 
−
        #content {
 
−
            width: 100%;
 
−
            padding: 0px;
 
−
            margin-top: -7px;
 
−
            margin-left: 0px;
 
−
        }
 
−
 
−
        body {
 
−
            background: white;
 
−
        }
 
−
 
−
        #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {
 
−
            margin-bottom: 0;
 
−
            color: #F8CE63;
 
−
        }
 
−
 
−
        table {;
 
−
        }
 
−
 
−
        /**************************************************************** MENU ***************************************************************/
 
−
        /* Wrapper for the menu */
 
−
        .igem_2017_menu_wrapper {
 
−
            width: 15%;
 
−
            height: 100vh;
 
−
            position: fixed;
 
−
            right: 0;
 
−
            padding: 0;
 
−
            float: right;
 
−
            border-left: 1px solid #F8CE63;
 
−
            background-color: white;
 
−
            text-align: left;
 
−
            font-family: Tahoma, Geneva, sans-serif;
 
−
            overflow-y: auto;
 
−
            overflow-x: hidden;
 
−
        }
 
−
 
−
        /* this hides the scrollbar to keep view consistency */
 
−
        .igem_2017_menu_wrappe::-webkit-scrollbar {
 
−
            display: none;
 
−
        }
 
−
 
−
        /* styling for links in the menu, removes the line under text */
 
−
        .igem_2017_menu_wrapper a {
 
−
            text-decoration: none;
 
−
        }
 
−
 
−
        /* styling for the images in the menu */
 
−
        .igem_2017_menu_wrapper img {
 
−
            width: 100%;
 
−
        }
 
−
 
−
        /* styling for the menu buttons */
 
−
        .igem_2017_menu_wrapper .menu_button {
 
−
            width: 100%;
 
−
            padding: 10px 0px 10px 15px;
 
−
            float: left;
 
−
            border-bottom: 1px solid #F8CE63;
 
−
            font-size: 16px;
 
−
            font-weight: bold;
 
−
            color: #F8CE63;
 
−
            cursor: pointer;
 
−
        }
 
−
 
−
        .igem_2017_menu_wrapper .menu_bottom_padding {
 
−
            width: 100%;
 
−
            height: 30px;
 
−
            float: left;
 
−
        }
 
−
 
−
        .menu_button.direct_to_page {
 
−
            padding-left: 36px;
 
−
        }
 
−
 
−
        .igem_2017_menu_wrapper .menu_button .expand_collapse_icon {
 
−
            width: 10%;
 
−
            float: left;
 
−
        }
 
−
 
−
        .igem_2017_menu_wrapper .menu_button .expand_collapse_icon::before {
 
−
            content: "+";
 
−
        }
 
−
 
−
        /* styling for the menu buttons on hover */
 
−
        .igem_2017_menu_wrapper .menu_button:hover, .igem_2017_menu_wrapper .submenu_button:hover, .submenu_button.current_page:hover {
 
−
            background-color: #F8CE63;
 
−
            text-decoration: none;
 
−
            color: #190707;
 
−
        }
 
−
 
−
        /* styling for the menu button when it is the current page */
 
−
        .current_page {
 
−
            background-color: white !important;
 
−
            color: #F8CE63 !important;
 
−
        }
 
−
 
−
        /* styling for the submenu buttons */
 
−
        .igem_2017_menu_wrapper .submenu_button {
 
−
            width: 100%;
 
−
            padding: 10px 0px 10px 34px;
 
−
            float: left;
 
−
            background-color: white;
 
−
            border-bottom: 1px solid #F8CE63;
 
−
            font-size: 15px;
 
−
            color: #F8CE63;
 
−
            cursor: pointer;
 
−
        }
 
−
 
−
        /* wrapper for the submenu items, they are hidden by default*/
 
−
        .igem_2017_menu_wrapper .submenu_wrapper {
 
−
            display: none;
 
−
        }
 
−
 
−
        /* when the page size is bigger than 800px, this show/hide control is hidden by default */
 
−
        .igem_2017_menu_wrapper #display_menu_control {
 
−
            display: none;
 
−
            text-align: center;
 
−
        }
 
−
 
−
        /***************************************************** CONTENT OF THE PAGE ****************************************************/
 
−
 
−
        /* Wrapper for the content */
 
−
        .igem_2017_content_wrapper {
 
−
            width: 83%;
 
−
            margin: 0;
 
−
            display: block;
 
−
            float: left;
 
−
            background-color: white;
 
−
            font-family: Tahoma, Geneva, sans-serif;
 
−
        }
 
−
 
−
        /********************************* HTML STYLING  *********************************/
 
−
 
−
        /* styling for the titles h1 h2 */
 
−
        .igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 {
 
−
            color: #F8CE63;
 
−
            padding: 15px 0 25px 0;
 
−
            border-bottom: 0;
 
−
        }
 
−
 
−
        /* styling for the titles  h3 h4 h5 h6*/
 
−
        .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 {
 
−
            padding: 5px 15px;
 
−
            border-bottom: 0;
 
−
        }
 
−
 
−
        /* font and text */
 
−
        .container p,
 
−
        #HQ_page p {
 
−
            line-height: 24px;
 
−
            font-size: 16px;
 
−
            color: #333;
 
−
        }
 
−
 
−
        .igem_2017_content_wrapper img {
 
−
            padding: 15px 0;
 
−
        }
 
−
 
−
        /* Links */
 
−
        .igem_2017_content_wrapper a {
 
−
            font-weight: bold;
 
−
            text-decoration: underline;
 
−
            text-decoration-color: #190707;
 
−
            color: #3399ff;
 
−
            -webkit-transition: all 0.4s ease;
 
−
            -moz-transition: all 0.4s ease;
 
−
            -ms-transition: all 0.4s ease;
 
−
            -o-transition: all 0.4s ease;
 
−
            transition: all 0.4s ease;
 
−
        }
 
−
 
−
        /* hover for the links */
 
−
        .igem_2017_content_wrapper a:hover {
 
−
            text-decoration: none;
 
−
            color: #000000;
 
−
        }
 
−
 
−
        /* non numbered lists */
 
−
        .igem_2017_content_wrapper ul {
 
−
            padding: 0px 20px;
 
−
            font-size: 15px;
 
−
            font-family: Tahoma, Geneva, sans-serif;
 
−
        }
 
−
 
−
        /* numbered lists */
 
−
        .igem_2017_content_wrapper ol {
 
−
            padding: 0px;
 
−
            font-size: 15px;
 
−
            font-family: Tahoma, Geneva, sans-serif;
 
−
        }
 
−
 
−
        /* Table */
 
−
        .igem_2017_content_wrapper table {
 
−
            width: 97%;
 
−
            margin: 15px 10px;
 
−
            border: 2px solid #190707;
 
−
            border-collapse: collapse;
 
−
        }
 
−
 
−
        /* table cells */
 
−
        .igem_2017_content_wrapper td {
 
−
            padding: 10px;
 
−
            vertical-align: text-top;
 
−
            border: 1px solid #190707;
 
−
            border-collapse: collapse;
 
−
        }
 
−
 
−
        /* table headers */
 
−
        .igem_2017_content_wrapper th {
 
−
            padding: 10px;
 
−
            vertical-align: text-top;
 
−
            border: 1px solid #190707;
 
−
            border-collapse: collapse;
 
−
 
−
        }
 
−
 
−
        /**********************************LAYOUT CLASSES **********************************/
 
−
 
−
        /* general class for column divs */
 
−
        .igem_2017_content_wrapper .column {
 
−
            padding: 10px 0px;
 
−
            float: left;
 
−
        }
 
−
 
−
        /* class for a full width column */
 
−
        .column .full_size {
 
−
            width: 100%;
 
−
        }
 
−
 
−
        /* styling for images in a full width column*/
 
−
        .column.full_size img {
 
−
            width: 97%;
 
−
            padding: 10px 15px;
 
−
        }
 
−
 
−
        /* class for a half width column */
 
−
        .column.half_size {
 
−
            width: 50%;
 
−
        }
 
−
 
−
        /* styling for images in a half width column*/
 
−
        .column.half_size img {
 
−
            width: 94.5%;
 
−
            padding: 10px 15px;
 
−
        }
 
−
 
−
        /********************************* SUPPORT CLASSES ********************************/
 
−
 
−
        /* class that clears content below*/
 
−
        .igem_2017_content_wrapper .clear {
 
−
            clear: both;
 
−
        }
 
−
 
−
        /* adds extra spacing when clearing content */
 
−
        .igem_2017_content_wrapper .clear.extra_space {
 
−
            height: 30px;
 
−
        }
 
−
 
−
        /* highlight class, makes content slightly smaller */
 
−
        .igem_2017_content_wrapper .highlight {
 
−
            margin: 0px 15px;
 
−
            padding: 15px 0px;
 
−
        }
 
−
 
−
        /* highlight class, adds a gray background */
 
−
        .igem_2017_content_wrapper .highlight.gray {
 
−
            background-color: #190707;
 
−
        }
 
−
 
−
        /* highlight with decoration blue line on top */
 
−
        .igem_2017_content_wrapper .highlight.blue_top {
 
−
            border-top: 4px solid #190707;
 
−
        }
 
−
 
−
        /* highlight with a full blue border decoration */
 
−
        .igem_2017_content_wrapper .highlight.blue_border {
 
−
            border: 4px solid #190707;
 
−
        }
 
−
 
−
        /* button class */
 
−
        .igem_2017_content_wrapper .button {
 
−
            max-width: 35%;
 
−
            margin: 30px auto;
 
−
            padding: 12px 10px;
 
−
            background-color: #190707;
 
−
            text-align: center;
 
−
            color: #F8CE63;
 
−
            -webkit-transition: all 0.4s ease;
 
−
            -moz-transition: all 0.4s ease;
 
−
            -ms-transition: all 0.4s ease;
 
−
            -o-transition: all 0.4s ease;
 
−
            transition: all 0.4s ease;
 
−
        }
 
−
 
−
        /* styling for button on hover */
 
−
        .igem_2017_content_wrapper .button:hover {
 
−
            background-color: #190707;
 
−
            color: #F8CE63;
 
−
        }
 
−
 
−
        /***************************************************** RESPONSIVE STYLING ****************************************************/
 
−
 
−
        *, *:before, *:after {
 
−
            -webkit-box-sizing: border-box;
 
−
            -moz-box-sizing: border-box;
 
−
            box-sizing: border-box;
 
−
        }
 
−
 
−
        body {
 
−
            overflow-x: hidden;
 
−
            margin-top: 80px;
 
−
            letter-spacing: 0.02em;
 
−
        }
 
−
 
−
        /* IF THE SCREEN IS LESS THAN 1200PX */
 
−
        @media only screen and (max-width: 1024px) {
 
−
 
−
            #content {
 
−
                width: 100%;
 
−
            }
 
−
 
−
            .igem_2017_content_wrapper {
 
−
                width: 100%;
 
−
            }
 
−
 
−
            .igem_2017_menu_wrapper {
 
−
                width: 100%;
 
−
                height: 15%;
 
−
                position: relative;
 
−
                left: 0;
 
−
            }
 
−
 
−
            .highlight {
 
−
                padding: 10px 0px;
 
−
            }
 
−
 
−
            .igem_2017_menu_wrapper #display_menu_control {
 
−
                display: none;
 
−
            }
 
−
 
−
            #menu_content {
 
−
                display: block;
 
−
            }
 
−
 
−
            .menu_button.direct_to_page {
 
−
                padding-left: 17px;
 
−
            }
 
−
        }
 
−
 
−
        /* IF THE SCREEN IS LESS THAN 800PX */
 
−
        @media only screen and (max-width: 800px) {
 
−
 
−
            .igem_2017_content_wrapper {
 
−
                width: 100%;
 
−
                margin-left: 0;
 
−
            }
 
−
 
−
            .column.half_size {
 
−
                width: 100%;
 
−
            }
 
−
 
−
            .column.full_size img, .column.half_size img {
 
−
                width: 100%;
 
−
                padding: 10px 0;
 
−
            }
 
−
 
−
            .highlight {
 
−
                padding: 15px 5px;
 
−
            }
 
−
 
−
            .igem_2017_menu_wrapper #display_menu_control {
 
−
                display: block;
 
−
            }
 
−
 
−
            #menu_content {
 
−
                display: none;
 
−
            }
 
−
 
−
            .igem_2017_menu_wrapper .menu_button .expand_collapse_icon {
 
−
                width: 5%;
 
−
            }
 
−
 
−
            .menu_bottom_padding {
 
−
                display: none;
 
−
            }
 
−
 
−
            .menu_button.direct_to_page {
 
−
                padding-left: 36px;
 
−
            }
 
−
        }
 
−
 
−
        /* special class that the system uses to make sure the team wants a page to be evaluated */
 
−
        .judges-will-not-evaluate {
 
−
            width: 96.6%;
 
−
            margin: 5px 15px;
 
−
            display: block;
 
−
            border: 4px solid #3399ff;
 
−
            font-weight: bold;
 
−
        }
 
−
 
−
        /* CUSTOM BS NAVBAR STYLES */
 
−
        .navbar {
 
−
            border: 0;
 
−
        }
 
−
 
−
        .navbar-default {
 
−
            margin-top: 15px;
 
−
            background-color: #333;
 
−
        }
 
−
 
−
        .navbar-brand {
 
−
            padding: 0;
 
−
            padding-left: 25px;
 
−
            padding-right: 25px;
 
−
        }
 
−
 
−
        .navbar-brand img {
 
−
            height: 49px;
 
−
        }
 
−
 
−
        .navbar-default .navbar-nav > li > a {
 
−
            color: white;
 
−
        }
 
−
 
−
        .navbar-default .navbar-nav > li > a:focus,
 
−
        .navbar-default .navbar-nav > li > a:hover,
 
−
        .navbar-default .navbar-nav > .dropdown > a .caret:hover {
 
−
            color: #F8CE63;
 
−
        }
 
−
 
−
        .navbar-default .navbar-nav > .open > a,
 
−
        .navbar-default .navbar-nav > .open > a:hover,
 
−
        .navbar-default .navbar-nav > .open > a:focus {
 
−
            color: #F8CE63;
 
−
            background-color: #444;
 
−
        }
 
−
 
−
        .navbar-default .navbar-nav > .dropdown > a .caret {
 
−
            border-top-color: #eee;
 
−
            border-bottom-color: #eee;
 
−
        }
 
−
 
−
        .dropdown-menu {
 
−
            border: 0;
 
−
        }
 
−
        .dropdown-menu > li > a {
 
−
            color: white;
 
−
        }
 
−
 
−
        .dropdown-menu > li > a:hover,
 
−
        .dropdown-menu > li > a:focus {
 
−
            color: #111;
 
−
            text-decoration: none;
 
−
            background-color: #F8CE63;
 
−
        }
 
−
 
−
        .navbar-default .navbar-nav > .dropdown > a:hover .caret,
 
−
        .navbar-default .navbar-nav > .dropdown > a:focus .caret {
 
−
            border-top-color: white;
 
−
            border-bottom-color: white;
 
−
        }
 
−
 
−
        @media (max-width: 767px) {
 
−
            .navbar-default .navbar-nav .open .dropdown-menu>li>a {
 
−
                color: #ddd;
 
−
            }
 
−
            .navbar-default .navbar-nav .open .dropdown-menu>li>a:focus,
 
−
            .navbar-default .navbar-nav .open .dropdown-menu>li>a:hover {
 
−
                color: #F8CE63;
 
−
            }
 
−
        }
 
−
 
−
        .navbar-nav>li>.dropdown-menu {
 
−
            background-color: #444;
 
−
        }
 
−
 
−
        .section {
 
−
            margin: 25px 0;
 
−
        }
 
−
 
−
    </style>
 
−
    <script src="https://code.jquery.com/jquery-1.10.2.min.js"></script>
 
−
    <script src="https://netdna.bootstrapcdn.com/bootstrap/3.0.0/js/bootstrap.min.js"></script>
 
−
 
−
    <script>
 
−
        $(document).ready(function(){
 
−
            $("#mw-content-text").removeClass("mw-content-ltr");
 
−
        });
 
−
    </script>
 
−
 
−
 
−
    <!-- This tells the browser that your page is responsive -->
 
−
    <meta name="viewport" content="width=device-width, initial-scale=1">
 
−
 
−
</head>
 
−
<body>
 
−
 
−
<!--MENU-->
 
−
<nav class="navbar navbar-default navbar-fixed-top">
 
−
    <div class="container-fluid">
 
−
        <!-- Brand and toggle get grouped for better mobile display -->
 
−
        <div class="navbar-header">
 
−
            <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
 
−
                    data-target="#bs-example-navbar-collapse-1" aria-expanded="false">
 
−
                <span class="sr-only">Toggle navigation</span>
 
−
                <span class="icon-bar"></span>
 
−
                <span class="icon-bar"></span>
 
−
                <span class="icon-bar"></span>
 
−
            </button>
 
−
            <a class="navbar-brand" href="https://2017.igem.org/Team:Stuttgart">
 
−
                <img alt="Team:Stuttgart"
 
−
                    src="https://static.igem.org/mediawiki/2017/1/18/UNISTUTTGARTLOGO.png">
 
−
            </a>
 
−
        </div>
 
−
 
−
        <!-- Collect the nav links, forms, and other content for toggling -->
 
−
        <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
 
−
            <ul class="nav navbar-nav">
 
−
                <li>
 
−
                    <a href="https://2017.igem.org/Team:Stuttgart">Home</a>
 
−
                </li>
 
−
                <li class="dropdown">
 
−
                    <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true"
 
−
                      aria-expanded="false">Team <span class="caret"></span></a>
 
−
                    <ul class="dropdown-menu">
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Team">Team</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Attributions">Attributions</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Sponsoring">Sponoring</a></li>
 
−
                        <!--<li role="separator" class="divider"></li>-->
 
−
                    </ul>
 
−
                </li>
 
−
                <li class="dropdown">
 
−
                    <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true"
 
−
                      aria-expanded="false">Project <span class="caret"></span></a>
 
−
                    <ul class="dropdown-menu">
 
−
                        <!--<li class="navbar-text navbar-left">Dry Lab</li>-->
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Description">Description</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Background-Information">Background
 
−
                            Information</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Protocols">Protocols & Experiments</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Notebook">Notebook</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/InterLab">Interlab Study</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Model">Modelling</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Results">Results</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Parts">Parts</a></li>
 
−
                        <!--<li role="separator" class="divider"></li>-->
 
−
                    </ul>
 
−
                </li>
 
−
                <li>
 
−
                    <a href="https://2017.igem.org/Team:Stuttgart/Safety">Safety</a>
 
−
                </li>
 
−
                <li class="dropdown">
 
−
                    <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true"
 
−
                      aria-expanded="false">Human Practices <span class="caret"></span></a>
 
−
                    <ul class="dropdown-menu">
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Engagement">Public Engagement</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/HP/Gold_Integrated">Integrated and Gold</a>
 
−
                        </li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/HP/Silver">Silver HP</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Collaborations">Collaborations</a></li>
 
−
                    </ul>
 
−
                </li>
 
−
                <li class="dropdown">
 
−
                    <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true"
 
−
                      aria-expanded="false">Awards <span class="caret"></span></a>
 
−
                    <ul class="dropdown-menu">
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Applied_Design">Applied Design</a></li>
 
−
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Measurement">Measurement</a></li>
 
−
                    </ul>
 
−
                </li>
 
−
                <li>
 
−
                    <a href="https://igem.org/2017_Judging_Form?team=Stuttgart">Judging Form</a>
 
−
                </li>
 
−
            </ul>
 
−
        </div><!-- /.navbar-collapse -->
 
−
    </div><!-- /.container-fluid -->
 
−
</nav>
 
  
 
<!-- start of content -->
 
<!-- start of content -->
−
<h1 align=middle>Interlab Study</h1>
+
<h1 align=middle>InterLab Study</h1>
 
<div class="container">
 
<div class="container">
 
       <div class="row section">
 
       <div class="row section">
−
         <div class="col-xs-12 col-sm-8 col-md-8">
+
         <div class="col-xs-12 col-sm-12 col-md-12">
 
           <h2>Introduction</h2>
 
           <h2>Introduction</h2>
−
<br>
+
        </div>
 +
      </div>
 +
      <div class="row section">
 +
        <div class="col-xs-12 col-sm-12 col-md-12">
 
             <p>Reproducibility of an experiment and reliable measurement results are important parts of synthetic biology but it is very complicated to find a significant number of laboratories that are able to work on a identical protocol to produce comparable results.  
 
             <p>Reproducibility of an experiment and reliable measurement results are important parts of synthetic biology but it is very complicated to find a significant number of laboratories that are able to work on a identical protocol to produce comparable results.  
 
               Being in many places in the world iGEM seems to be the perfect platform to compare results of one experiment in many different laboratories.
 
               Being in many places in the world iGEM seems to be the perfect platform to compare results of one experiment in many different laboratories.
−
iGEM Stuttgart also took part at the fourth InterLab Measurement Study in 2017.  
+
              iGEM Stuttgart also took part at the fourth InterLab Measurement Study in 2017.  
−
The aim of this years study was to measure GFP fluorescence of 6 different test devices (+negative and positive control).  
+
              The aim of this years study was to measure GFP fluorescence of 6 different test devices (+negative and positive control).  
−
The following eight constructs on the right side (iGEM-kit plate 7) were transformed into chemo-competent DH5α E.coli cells.
+
              The eight constructs (iGEM-kit plate 7) listed below were transformed into chemo-competent DH5α <i>E. coli</i> cells.</p>
 +
          </div>
 +
        </div>
 +
    <div class="row section">   
 +
<div class="col-xs-12 col-sm-5 col-md-5">
 +
    <ul>
 +
      <li>Positive Control (<a href="http://parts.igem.org/Part:BBa_120270">BBa_120270</a>)</li>
 +
      <li>Negative Control (<a href="http://parts.igem.org/Part:BBa_R0040">BBa_R0040</a>)</li>
 +
      <li>Test Device 1 (<a href="http://parts.igem.org/Part:BBa_J364000">BBa_J364000</a>)</li>
 +
      <li>Test Device 2 (<a href="http://parts.igem.org/Part:BBa_J364001">BBa_J364001</a>)</li>
 +
      <li>Test Device 3 (<a href="http://parts.igem.org/Part:BBa_J364002">BBa_J364002</a>)</li>
 +
      <li>Test Device 4 (<a href="http://parts.igem.org/Part:BBa_J364003">BBa_J364003</a>)</li>
 +
      <li> Test Device 5 (<a href="http://parts.igem.org/Part:BBa_J364004">BBa_J364004</a>)</li>
 +
      <li>Test Device 6 (<a href="http://parts.igem.org/Part:BBa_J364005">BBa_J364005</a>)</li>
 +
  </ul>
 +
</div>
 +
<div class="col-xs-12 col-sm-7 col-md-7">     
 +
<div class="thumb tleft">
 +
<div class="thumbinner" style="width:400px;">
 +
<a href="https://static.igem.org/mediawiki/2017/3/39/Workflow_interlab.png" class="image">
 +
<img alt="" src="https://static.igem.org/mediawiki/2017/3/39/Workflow_interlab.png" width="380" height="200" class="thumbimage" />
 +
</a> 
 +
<div class="thumbcaption">
 +
<div class="magnify">
 +
<a href="https://static.igem.org/mediawiki/2017/3/39/Workflow_interlab.png" class="internal" title="Enlarge">
 +
  <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" />
 +
</a>
 +
</div>
 +
<b>Figure 1:</b> Workflow InterLab Study, from InterLab_2017_Plate_Reader_Protocol.
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="row section">
 +
  <div class="col-xs-12 col-sm-12 col-md-12">
 +
<p>
 
First step of the experiment is the calibration of the available plate reader with LUDOX SH40 and the generation of a standard curve with fluorescein (both provided in the iGEM-Measurement Kit).  
 
First step of the experiment is the calibration of the available plate reader with LUDOX SH40 and the generation of a standard curve with fluorescein (both provided in the iGEM-Measurement Kit).  
−
The next step is the transformation of the mentioned plasmids into DH5alpha E.coli cells followed by the procedure explained in the picture below.
+
The next step is the transformation of the mentioned plasmids into DH5alpha <i>E. coli</i> cells followed by the procedure explained in the picture below.
 
Results of the measurements of OD600 and fluorescence after 0, 2, 4 and 6 hours will be compared and used to establish a precise GFP measurement protocol.  
 
Results of the measurements of OD600 and fluorescence after 0, 2, 4 and 6 hours will be compared and used to establish a precise GFP measurement protocol.  
 
We used a BioTek Synergy 2 plate reader and clear 96-well plates for both measurements.  
 
We used a BioTek Synergy 2 plate reader and clear 96-well plates for both measurements.  
Line 617: Line 60:
 
</p>
 
</p>
 
</div>
 
</div>
−
<div class="col-xs-12 col-sm-4 col-md-4">
 
−
<br>
 
−
<br>
 
−
<br>
 
−
<table>
 
−
  <tr>
 
−
        <td>Positive Control (BBa_120270)</td></tr>
 
−
      <tr> <td>Negative Control (BBa_R0040)</td></tr>
 
−
      <tr><td>Test Device 1 (BBa_J364000)</td></tr>
 
−
      <tr> <td>Test Device 2 (BBa_J364001)</td></tr>
 
−
      <tr><td>Test Device 3 (BBa_J364002)</td></tr>
 
−
        <tr><td>Test Device 4 (BBa_J364003)</td></tr>
 
−
      <tr> <td> Test Device 5 (BBa_J364004)</td></tr>
 
−
      <tr> <td>Test Device 6 (BBa_J364005)</td></tr>
 
−
</table>
 
 
</div>
 
</div>
 
</div>
 
</div>
−
<div class="row section">
+
 
−
    <div class="col-xs-12 col-sm-12 col-md-12">
+
 
−
        <!--<img src="https://static.igem.org/mediawiki/2017/6/6b/TeamStuttgart_workflow_platereader2">-->
+
 
−
        <img src="https://static.igem.org/mediawiki/2017/6/6b/TeamStuttgart_workflow_platereader2.png" class="img-responsive"/>
+
 
−
    </div>
+
 
−
  </div>
+
<div class="container">  
−
</div>
+
−
<br>
+
−
<br>
+
−
<div class="container">
+
 
       <div class="row section">
 
       <div class="row section">
−
         <div class="col-xs-12 col-sm-7 col-md-7">
+
         <div class="col-xs-12 col-sm-12 col-md-12">
−
          <h2>Results and Discussion</h2>
+
<h2>Results and Discussion</h2>
−
<br>
+
−
<br>
+
 
           <h3>LUDOX-OD600 Measurement (calibration)</h3>
 
           <h3>LUDOX-OD600 Measurement (calibration)</h3>
−
<br>
+
        </div>
−
             <p>HIER TEXT REINKOPIEREN</p>
+
      </div>
 +
      <div class="row section">
 +
        <div class="col-xs-12 col-sm-5 col-md-5">
 +
             <p>For the calibration 100 μL of LUDOX-S40 from the InterLab Measurement Kit was added into the wells A1, B1, C1, and D1. Analogical 100 μL of ddH<sub>2</sub>O was added to the wells A2, B2, C2, D2.
 +
              The measurement was performed with a plate reader at an absorbance of 600 nm.
 +
</p>
 
</div>
 
</div>
−
<div class="col-xs-12 col-sm-5 col-md-5">
+
<div class="col-xs-12 col-sm-7 col-md-7">
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
 
   <table>  
 
   <table>  
−
     <tr> <th></th> <th>LUDOX-HS40</th><th>H2O</th> </tr>
+
     <tr> <th></th> <th>LUDOX-HS40</th><th>H<sub>2</sub>O</th> </tr>
 
     <tr><td>Replicate 1</td><td>0,04</td><td>0,026</td></tr>
 
     <tr><td>Replicate 1</td><td>0,04</td><td>0,026</td></tr>
 
     <tr><td>Replicate 2</td><td>0,041</td><td>0,027</td></tr>
 
     <tr><td>Replicate 2</td><td>0,041</td><td>0,027</td></tr>
Line 674: Line 95:
 
</div>
 
</div>
 
<div class="row section">
 
<div class="row section">
−
  <div class="col-xs-12 col-sm-4 col-md-4">
+
    <div class="col-xs-12 col-sm-12 col-md-12">
−
    <h3>Fluorescein-Fluorescence-Measurement (standard curve)</h3>
+
<h3>Fluorescein-Fluorescence-Measurement (standard curve)</h3>
−
<br>
+
</div>
 +
</div>
 +
<div class="row section">
 +
  <div class="col-xs-12 col-sm-12 col-md-12">
 
<p>Measurement of different fluorescein concentrations.
 
<p>Measurement of different fluorescein concentrations.
 
excitation wavelength: 485nm; emission wavelength: 528nm</p>
 
excitation wavelength: 485nm; emission wavelength: 528nm</p>
−
    </div>
 
−
    <div class="col-xs-12 col-sm-8 col-md-8">
 
−
<br>
 
−
<br>
 
−
<br>
 
−
<br>
 
−
        <!--<img src="https://static.igem.org/mediawiki/2017/4/4b/TeamStuttgart_fluorescin.png">-->
 
−
        <img src="https://static.igem.org/mediawiki/2017/4/4b/TeamStuttgart_fluorescin.png" class="img-responsive"/>
 
 
     </div>
 
     </div>
 
   </div>
 
   </div>
−
<br>
 
 
   <div class="row section">
 
   <div class="row section">
−
     <div class="col-xs-12 col-sm-4 col-md-4">
+
     <div class="col-xs-12 col-sm-6 col-md-6">
−
      <h3>Transformation</h3>
+
        <!--<img src="https://static.igem.org/mediawiki/2017/6/63/Interlab1.png">-->
−
<br>
+
        <img src="https://static.igem.org/mediawiki/2017/8/8d/Interabfluoro4.png" class="img-responsive"/>
−
   <p>We had at least two colonies of every test device except for test device 1 (transformation not successful).
+
    </div>
−
All of the following measurements are without test device 1. Agar-plates with colonies after transformation.</p>
+
</div>
 +
  <div class="row section">
 +
    <div class="col-xs-12 col-sm-12 col-md-12">
 +
<h3>Transformation</h3>
 +
   <p> We had at least two colonies of every test device except for test device 1 (transformation not successful).
 +
All of the following measurements are without test device 1. </p>
 
       </div>
 
       </div>
−
       <div class="col-xs-12 col-sm-8 col-md-8">
+
    </div>
−
<br>
+
    <div class="row section">
−
<br>
+
       <div class="col-xs-12 col-sm-12 col-md-12">
−
<br>
+
 
           <!--<img src="https://static.igem.org/mediawiki/2017/3/3b/TeamStuttgart_agarplates.jpeg">-->
 
           <!--<img src="https://static.igem.org/mediawiki/2017/3/3b/TeamStuttgart_agarplates.jpeg">-->
 
           <img src="https://static.igem.org/mediawiki/2017/3/3b/TeamStuttgart_agarplates.jpeg" class="img-responsive"/>
 
           <img src="https://static.igem.org/mediawiki/2017/3/3b/TeamStuttgart_agarplates.jpeg" class="img-responsive"/>
 
       </div>
 
       </div>
 
     </div>
 
     </div>
−
<br>
 
 
     <div class="row section">
 
     <div class="row section">
 
       <div class="col-xs-12 col-sm-6 col-md-6">
 
       <div class="col-xs-12 col-sm-6 col-md-6">
−
        <h3>Cell Measurement</h3>
+
<h3>Cell Measurement</h3>
−
<br>
+
     <p>OD600-measurement - growth of <i>E. coli cells</i>.<br>All cells (with different parts) show the expectable exponential growth.</p>
−
     <p>OD600-measurement - growth of E.coli cells</p>
+
−
        </div>
+
−
        <div class="col-xs-12 col-sm-6 col-md-6">
+
−
<br>
+
−
<br>
+
−
<br>
+
 
             <!--<img src="https://static.igem.org/mediawiki/2017/1/1c/TeamStuttgart_bactgrowth.png">-->
 
             <!--<img src="https://static.igem.org/mediawiki/2017/1/1c/TeamStuttgart_bactgrowth.png">-->
−
             <img src="https://static.igem.org/mediawiki/2017/1/1c/TeamStuttgart_bactgrowth.png" class="img-responsive"/>
+
             <img src="https://static.igem.org/mediawiki/2017/6/64/Interabfluoro5.png" class="img-responsive"/>
 
         </div>
 
         </div>
−
      </div>
 
−
<br>
 
−
      <div class="row section">
 
 
         <div class="col-xs-12 col-sm-6 col-md-6">
 
         <div class="col-xs-12 col-sm-6 col-md-6">
−
          <h3>Fluorescence Measurement of E.coli cells</h3>
+
<h3>Fluorescence Measurement of <i>E. coli</i> cells</h3>
−
<br>
+
       <p>Test device 2, the positive control and test device 4 show the highest fluorescence after 6 hours followed by test device 5. The remaining test devices do not show any increasing fluorescence and stay on the level of the negative control.
−
       <p>Excitation wavelength: 485nm; emission wavelength: 528nm</p>
+
        (Excitation wavelength: 485nm; emission wavelength: 528nm)</p>
−
          </div>
+
−
          <div class="col-xs-12 col-sm-6 col-md-6">
+
−
<br>
+
−
<br>
+
−
<br>
+
 
               <!--<img src="https://static.igem.org/mediawiki/2017/f/f2/TeamStuttgart_fluokurven.png">-->
 
               <!--<img src="https://static.igem.org/mediawiki/2017/f/f2/TeamStuttgart_fluokurven.png">-->
−
               <img src="https://static.igem.org/mediawiki/2017/f/f2/TeamStuttgart_fluokurven.png" class="img-responsive"/>
+
               <img src="https://static.igem.org/mediawiki/2017/2/27/Interabfluoro6.png" class="img-responsive"/>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
Line 738: Line 142:
  
  
−
</body>
+
 
 
</html>
 
</html>

Latest revision as of 22:30, 14 December 2017

InterLab Study

Introduction

Reproducibility of an experiment and reliable measurement results are important parts of synthetic biology but it is very complicated to find a significant number of laboratories that are able to work on a identical protocol to produce comparable results. Being in many places in the world iGEM seems to be the perfect platform to compare results of one experiment in many different laboratories. iGEM Stuttgart also took part at the fourth InterLab Measurement Study in 2017. The aim of this years study was to measure GFP fluorescence of 6 different test devices (+negative and positive control). The eight constructs (iGEM-kit plate 7) listed below were transformed into chemo-competent DH5α E. coli cells.

Figure 1: Workflow InterLab Study, from InterLab_2017_Plate_Reader_Protocol.

First step of the experiment is the calibration of the available plate reader with LUDOX SH40 and the generation of a standard curve with fluorescein (both provided in the iGEM-Measurement Kit). The next step is the transformation of the mentioned plasmids into DH5alpha E. coli cells followed by the procedure explained in the picture below. Results of the measurements of OD600 and fluorescence after 0, 2, 4 and 6 hours will be compared and used to establish a precise GFP measurement protocol. We used a BioTek Synergy 2 plate reader and clear 96-well plates for both measurements. You can find the protocol, the detailed description of the experiment and information about the InterLab Study itself here.

Results and Discussion

LUDOX-OD600 Measurement (calibration)

For the calibration 100 μL of LUDOX-S40 from the InterLab Measurement Kit was added into the wells A1, B1, C1, and D1. Analogical 100 μL of ddH2O was added to the wells A2, B2, C2, D2. The measurement was performed with a plate reader at an absorbance of 600 nm.

LUDOX-HS40H2O
Replicate 10,040,026
Replicate 20,0410,027
Replicate 30,0430,026
Replicate 40,040,028
Arith. Mean 0,041
Corrected Abs6000,0143
Reference OD6000,043
OD600/Abs6002,98

Fluorescein-Fluorescence-Measurement (standard curve)

Measurement of different fluorescein concentrations. excitation wavelength: 485nm; emission wavelength: 528nm

Transformation

We had at least two colonies of every test device except for test device 1 (transformation not successful). All of the following measurements are without test device 1.

Cell Measurement

OD600-measurement - growth of E. coli cells.
All cells (with different parts) show the expectable exponential growth.

Fluorescence Measurement of E. coli cells

Test device 2, the positive control and test device 4 show the highest fluorescence after 6 hours followed by test device 5. The remaining test devices do not show any increasing fluorescence and stay on the level of the negative control. (Excitation wavelength: 485nm; emission wavelength: 528nm)