Difference between revisions of "Team:Stuttgart/Composite Part"

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<div class="column full_size judges-will-not-evaluate">
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<h3>★  ALERT! </h3>
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<h1 align=middle> Composite Part </h1>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<div class="container">
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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            <h2>KerP from <i>Pseudomonas aeruginosa</i></h2>
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        <div class="row section">
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            <div class="col-xs-12 col-sm-6 col-md-6">         
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<p>Keratinases are proteolytic enzymes that are applied in agro-industrial, pharmaceutical and biomedicals fields (Satyanarayana et al. 2013).  
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  In our project we used keratinases for establishing a microbial tube cleaner.  
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  Hair mostly consists of alpha-keratin which is hydrolyzed by keratinases.  
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  By using the keratinases we want to avoid chemical compounds that are recently present in tube cleaners.
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<p>
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  Many different keratinases produced by different microorganisms (e.g. <i>Pseudomonas aeruginosa</i>,<i>Bacillus subtilis</i>) have been reported.
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  These enzymes vary in their biochemical and biophysical properties e.g. temperature and pH activity range.
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  KerP is a keratinase originating from <i>Pseudomonas aeruginosa</i> It was succesfully transformed and expressed as an extracellular protein in <i>E.coli</i> with a size of 33 kDa and a specific activity of 3,7 kU/mg.
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  This keratinase belongs to the group of serine proteases with optimal activity at pH 9 and 50°C (Sharma and Gupta 2010).
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<p>
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Our construct
  
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<a href="http://parts.igem.org/Part:BBa_K2497999"> BBa_K2497999 </a>
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was based on a KerP sequence combined with an pBAD promotor which originates from <i>E.coli</i> and that is induced by L-arabinose. The utilized promotor can be also found in the iGEM registry (<a href="http://parts.igem.org/Part:BBa_K206000">BBa_K206000</a>). Since the KerP sequence was not provided at other part we synthesized this construct by IDT (Integrated DNA Technologies, BVBA, Belgium) and cloned it into the pSB1K3 backbone first. From there it was cloned into the pSB1C3 backbone and submitted to the iGEM registry afterwards. KerP gene sequence was taken from http://www.uniprot.org/. Gene sequence was optimized to eliminate illegal restriction sites. The construct was synthezied by IDT.
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</p>
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          <div class="thumb tleft">
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          <div class="thumbinner" style="width:550px;">
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          <a href="https://static.igem.org/mediawiki/2017/8/8e/PSB1C3-pBAD-kerP.png" class="image">
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            <img alt="" src="https://static.igem.org/mediawiki/2017/8/8e/PSB1C3-pBAD-kerP.png" width="530" height="480" class="thumbimage" />
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          </a> 
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          <div class="thumbcaption">
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            <div class="magnify">
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              <a href="https://static.igem.org/mediawiki/2017/8/8e/PSB1C3-pBAD-kerP.pngg" class="internal" title="Enlarge">
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                <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" />
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          </a>
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          <b>Figure 1:</b> plasmid map of kerP added with pBAD promotor BBa_K206000 cloned in pSB1C3 backbone.</div>
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<h1>Composite Parts</h1>
 
  
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<table width=40% align=center class="parts">
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<tr><br><br><br>
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<td><h4><b>PART NAME</b></h4></td>
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<td><h4><b>PART NUMBER</b></h4></td>
  
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<p>
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</tr>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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</p>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<tr>
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<td>pSB1C3 - pBAD - KerP </td>
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<td><a href="http://parts.igem.org/Part:BBa_K2497999"> BBa_K2497999 </a></td>
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</tr>
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</table>
  
 
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<h3>Best Composite Part Special Prize</h3>
 
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
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<br><br>
 
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
 
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<div class="highlight">
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<h4>Note</h4>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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Latest revision as of 22:32, 14 December 2017

Composite Part

KerP from Pseudomonas aeruginosa

Keratinases are proteolytic enzymes that are applied in agro-industrial, pharmaceutical and biomedicals fields (Satyanarayana et al. 2013). In our project we used keratinases for establishing a microbial tube cleaner. Hair mostly consists of alpha-keratin which is hydrolyzed by keratinases. By using the keratinases we want to avoid chemical compounds that are recently present in tube cleaners.

Many different keratinases produced by different microorganisms (e.g. Pseudomonas aeruginosa,Bacillus subtilis) have been reported. These enzymes vary in their biochemical and biophysical properties e.g. temperature and pH activity range. KerP is a keratinase originating from Pseudomonas aeruginosa It was succesfully transformed and expressed as an extracellular protein in E.coli with a size of 33 kDa and a specific activity of 3,7 kU/mg. This keratinase belongs to the group of serine proteases with optimal activity at pH 9 and 50°C (Sharma and Gupta 2010).

Our construct BBa_K2497999 was based on a KerP sequence combined with an pBAD promotor which originates from E.coli and that is induced by L-arabinose. The utilized promotor can be also found in the iGEM registry (BBa_K206000). Since the KerP sequence was not provided at other part we synthesized this construct by IDT (Integrated DNA Technologies, BVBA, Belgium) and cloned it into the pSB1K3 backbone first. From there it was cloned into the pSB1C3 backbone and submitted to the iGEM registry afterwards. KerP gene sequence was taken from http://www.uniprot.org/. Gene sequence was optimized to eliminate illegal restriction sites. The construct was synthezied by IDT.

Figure 1: plasmid map of kerP added with pBAD promotor BBa_K206000 cloned in pSB1C3 backbone.



PART NAME

PART NUMBER
pSB1C3 - pBAD - KerP BBa_K2497999