Difference between revisions of "Team:Pasteur Paris/Demonstrate"

(Prototype team page)
 
 
(36 intermediate revisions by 5 users not shown)
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{{Pasteur_Paris}}
+
<html lang="en">
 +
  <head>
 +
    <meta charset="utf-8">
 +
    <meta name="description" content="igem">
 +
    <meta name="keywords" content="igem, biology">
 +
    <meta name="copyright" content="© Copyright 2016 - iGEM Pasteur" />
 +
    <meta name="author" content="Pasteur">
 +
    <meta name="location" content="Paris, FRANCE">
 +
    <meta http-equiv="x-ua-compatible" content="ie=edge">
 +
    <meta name="viewport" content="width=device-width, initial-scale=1">
 +
    <link rel="icon" type="image/png" href="./static/favicon.png" />
 +
<head>
 +
<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css">
 +
<link rel="stylesheet" href="https://fonts.googleapis.com/icon?family=Material+Icons">
  
−
<html>
 
  
 +
<script>
  
 +
/*Fonction apparition onglet après click sur le menu*/
 +
function apparitionTeam() {
 +
    document.getElementById("Team").style.display = "block";
 +
    document.getElementById("Project").style.display = "none";
 +
    document.getElementById("Design").style.display = "none";
 +
    document.getElementById("Labwork").style.display = "none";
 +
    document.getElementById("HumanPractice").style.display = "none";
 +
    document.getElementById("Achievements").style.display = "none";
 +
}
 +
function apparitionProject() {
 +
    document.getElementById("Project").style.display = "block";
 +
    document.getElementById("Team").style.display = "none";
 +
    document.getElementById("Design").style.display = "none";
 +
    document.getElementById("Labwork").style.display = "none";
 +
    document.getElementById("HumanPractice").style.display = "none";
 +
    document.getElementById("Achievements").style.display = "none";
 +
}
 +
function apparitionDesign() {
 +
    document.getElementById("Design").style.display = "block";
 +
    document.getElementById("Team").style.display = "none";
 +
    document.getElementById("Project").style.display = "none";
 +
    document.getElementById("Labwork").style.display = "none";
 +
    document.getElementById("HumanPractice").style.display = "none";
 +
    document.getElementById("Achievements").style.display = "none";
 +
}
 +
function apparitionLabWork() {
 +
    document.getElementById("Labwork").style.display = "block";
 +
    document.getElementById("Team").style.display = "none";
 +
    document.getElementById("Project").style.display = "none";
 +
    document.getElementById("Design").style.display = "none";
 +
    document.getElementById("HumanPractice").style.display = "none";
 +
    document.getElementById("Achievements").style.display = "none";
 +
}
 +
function apparitionHumanPractice() {
 +
    document.getElementById("HumanPractice").style.display = "block";
 +
    document.getElementById("Team").style.display = "none";
 +
    document.getElementById("Project").style.display = "none";
 +
    document.getElementById("Design").style.display = "none";
 +
    document.getElementById("Labwork").style.display = "none";
 +
    document.getElementById("Achievements").style.display = "none";
 +
}
 +
function apparitionAchievements() {
 +
    document.getElementById("Achievements").style.display = "block";
 +
    document.getElementById("Team").style.display = "none";
 +
    document.getElementById("Project").style.display = "none";
 +
    document.getElementById("Design").style.display = "none";
 +
    document.getElementById("HumanPractice").style.display = "none";
 +
    document.getElementById("Labwork").style.display = "none";
 +
}
 +
/*////////////////////////////////////////////*/
  
−
<div class="column full_size judges-will-not-evaluate">
+
/* Fonction pour apparition barre rouge onglet*/
−
<h3>★  ALERT! </h3>
+
 
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
+
function apparitionbaton() {
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
    document.getElementById("batonbleu").style.display = "block";
 +
}
 +
 
 +
function deletebaton() {
 +
    document.getElementById("batonbleu").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton1() {
 +
    document.getElementById("batonbleu1").style.display = "block";
 +
}
 +
 
 +
function deletebaton1() {
 +
    document.getElementById("batonbleu1").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton1b() {
 +
    document.getElementById("batonbleu1b").style.display = "block";
 +
}
 +
 
 +
function deletebaton1b() {
 +
    document.getElementById("batonbleu1b").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton2() {
 +
    document.getElementById("batonbleu2").style.display = "block";
 +
}
 +
 
 +
function deletebaton2() {
 +
    document.getElementById("batonbleu2").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton3() {
 +
    document.getElementById("batonbleu3").style.display = "block";
 +
}
 +
 
 +
function deletebaton3() {
 +
    document.getElementById("batonbleu3").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton4() {
 +
    document.getElementById("batonbleu4").style.display = "block";
 +
}
 +
 
 +
function deletebaton4() {
 +
    document.getElementById("batonbleu4").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton5() {
 +
    document.getElementById("batonbleu5").style.display = "block";
 +
}
 +
 
 +
function deletebaton5() {
 +
    document.getElementById("batonbleu5").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton6() {
 +
    document.getElementById("batonbleu6").style.display = "block";
 +
}
 +
 
 +
function deletebaton6() {
 +
    document.getElementById("batonbleu6").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton7() {
 +
    document.getElementById("batonbleu7").style.display = "block";
 +
}
 +
 
 +
function deletebaton7() {
 +
    document.getElementById("batonbleu7").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton8() {
 +
    document.getElementById("batonbleu8").style.display = "block";
 +
}
 +
 
 +
function deletebaton8() {
 +
    document.getElementById("batonbleu8").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton8b() {
 +
    document.getElementById("batonbleu8b").style.display = "block";
 +
}
 +
 
 +
function deletebaton8b() {
 +
    document.getElementById("batonbleu8b").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton9() {
 +
    document.getElementById("batonbleu9").style.display = "block";
 +
}
 +
 
 +
function deletebaton9() {
 +
    document.getElementById("batonbleu9").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton10() {
 +
    document.getElementById("batonbleu10").style.display = "block";
 +
}
 +
 
 +
function deletebaton10() {
 +
    document.getElementById("batonbleu10").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton11() {
 +
    document.getElementById("batonbleu11").style.display = "block";
 +
}
 +
 
 +
function deletebaton11() {
 +
    document.getElementById("batonbleu11").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton12() {
 +
    document.getElementById("batonbleu12").style.display = "block";
 +
}
 +
 
 +
function deletebaton12() {
 +
    document.getElementById("batonbleu12").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton13() {
 +
    document.getElementById("batonbleu13").style.display = "block";
 +
}
 +
 
 +
function deletebaton13() {
 +
    document.getElementById("batonbleu13").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton14() {
 +
    document.getElementById("batonbleu14").style.display = "block";
 +
}
 +
 
 +
function deletebaton14() {
 +
    document.getElementById("batonbleu14").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton15() {
 +
    document.getElementById("batonbleu15").style.display = "block";
 +
}
 +
 
 +
function deletebaton15() {
 +
    document.getElementById("batonbleu15").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton16() {
 +
    document.getElementById("batonbleu16").style.display = "block";
 +
}
 +
 
 +
function deletebaton16() {
 +
    document.getElementById("batonbleu16").style.display = "none";
 +
}
 +
 
 +
/*////////////////////////////////////////////*/
 +
 
 +
function CarnotNoiretBlanc() {
 +
  document.getElementById("CarnotBW").style.display = "block";
 +
  document.getElementById("Carnot").style.display = "none";
 +
}
 +
 
 +
function CarnotCouleur() {
 +
  document.getElementById("Carnot").style.display = "block";
 +
  document.getElementById("CarnotBW").style.display = "none";
 +
}
 +
 
 +
 
 +
function GlobalCareNoiretBlanc() {
 +
  document.getElementById("GlobalCareBW").style.display = "block";
 +
  document.getElementById("GlobalCare").style.display = "none";
 +
}
 +
 
 +
function GlobalCareCouleur() {
 +
  document.getElementById("GlobalCare").style.display = "block";
 +
  document.getElementById("GlobalCareBW").style.display = "none";
 +
}
 +
 
 +
function MettlerToledoNoiretBlanc() {
 +
  document.getElementById("MettlerToledoBW").style.display = "block";
 +
  document.getElementById("MettlerToledo").style.display = "none";
 +
}
 +
 
 +
function MettlerToledoCouleur() {
 +
  document.getElementById("MettlerToledo").style.display = "block";
 +
  document.getElementById("MettlerToledoBW").style.display = "none";
 +
}
 +
 
 +
function geniousNoiretBlanc() {
 +
  document.getElementById("geniousBW").style.display = "block";
 +
  document.getElementById("genious").style.display = "none";
 +
}
 +
 
 +
function geniousCouleur() {
 +
  document.getElementById("genious").style.display = "block";
 +
  document.getElementById("geniousBW").style.display = "none";
 +
}
 +
 
 +
function centraleNoiretBlanc() {
 +
  document.getElementById("CentraleBW").style.display = "block";
 +
  document.getElementById("Centrale").style.display = "none";
 +
}
 +
 
 +
function centraleCouleur() {
 +
  document.getElementById("Centrale").style.display = "block";
 +
  document.getElementById("CentraleBW").style.display = "none";
 +
}
 +
 
 +
function ambassadeNoiretBlanc() {
 +
  document.getElementById("ambassadeBW").style.display = "block";
 +
  document.getElementById("ambassade").style.display = "none";
 +
}
 +
 
 +
function ambassadeCouleur() {
 +
  document.getElementById("ambassade").style.display = "block";
 +
  document.getElementById("ambassadeBW").style.display = "none";
 +
}
 +
 
 +
function QuiagenNoiretBlanc() {
 +
  document.getElementById("QuiagenBW").style.display = "block";
 +
  document.getElementById("Quiagen").style.display = "none";
 +
}
 +
 
 +
function QuiagenCouleur() {
 +
  document.getElementById("Quiagen").style.display = "block";
 +
  document.getElementById("QuiagenBW").style.display = "none";
 +
}
 +
 
 +
function eurofinsNoiretBlanc() {
 +
  document.getElementById("eurofinsBW").style.display = "block";
 +
  document.getElementById("eurofins").style.display = "none";
 +
}
 +
 
 +
function eurofinsCouleur() {
 +
  document.getElementById("eurofins").style.display = "block";
 +
  document.getElementById("eurofinsBW").style.display = "none";
 +
}
 +
 
 +
function ThermofisherNoiretBlanc() {
 +
  document.getElementById("ThermofisherBW").style.display = "block";
 +
  document.getElementById("Thermofisher").style.display = "none";
 +
}
 +
 
 +
function ThermofisherCouleur() {
 +
  document.getElementById("Thermofisher").style.display = "block";
 +
  document.getElementById("ThermofisherBW").style.display = "none";
 +
}
 +
 
 +
function BarthenteimNoiretBlanc() {
 +
  document.getElementById("BarthenteimBW").style.display = "block";
 +
  document.getElementById("Barthenteim").style.display = "none";
 +
}
 +
 
 +
function BarthenteimCouleur() {
 +
  document.getElementById("Barthenteim").style.display = "block";
 +
  document.getElementById("BarthenteimBW").style.display = "none";
 +
}
 +
 
 +
</script>
 +
<style>
 +
 
 +
@font-face {
 +
  font-family: "CrimsonTextSemi";
 +
  src: url("https://static.igem.org/mediawiki/2017/7/75/T--Pasteur_Paris--CrimsonText-Semibold.ttf");
 +
}
 +
 
 +
@font-face {
 +
  font-family: "CrimsonTextRoman";
 +
  src: url("https://static.igem.org/mediawiki/2017/0/0e/T--Pasteur_Paris--CrimsonText-Roman.ttf");
 +
}
 +
 
 +
body{
 +
  margin:30px;
 +
  padding:30px;
 +
  background-color:white;
 +
  height:100%;
 +
}
 +
 
 +
a{
 +
  text-decoration: none;
 +
  color:black;
 +
}
 +
 
 +
li{
 +
  list-style:none;
 +
}
 +
 
 +
.left{
 +
  left: 30px;
 +
  margin-bottom: 7.5px;
 +
  padding-top: 37.5px;
 +
  position:fixed;
 +
  background-color: none;
 +
  top:0;
 +
}
 +
 
 +
.right{
 +
  right: 30px;
 +
  margin-bottom: 7.5px; 
 +
  padding-top: 37.5px;
 +
  position:fixed;
 +
  background-color: none;
 +
  top:0;
 +
}
 +
 
 +
.center{
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
}
 +
 
 +
/*Position menu*/
 +
.menu { 
 +
    text-align: left;
 +
    width: 450px;
 +
    position: fixed;
 +
    left:30px;
 +
    line-height: 200%;
 +
    font-family: Helvetica, sans-serif;
 +
    font-weight: bold;
 +
    font-size: 7.5pt;
 +
    display: flex;
 +
    flex-direction: column-reverse;
 +
    text-align: bottom left;
 +
    bottom: 4%;
 +
}
 +
 
 +
.menu span{
 +
  cursor: pointer;
 +
}
 +
 
 +
.com{
 +
    display: flex;
 +
    flex-direction: column;
 +
    position:fixed;
 +
    text-align: left;
 +
    right:30px;
 +
    bottom: 4%;
 +
}
 +
 
 +
/*Ensemble pour trait après le menu*/
 +
 
 +
.lastword {
 +
    width: 150px;
 +
}
 +
 
 +
.lastword img {
 +
    position:absolute;
 +
    opacity:0;
 +
}
 +
 
 +
.lastword:hover:after{
 +
    display:inline-block;
 +
    vertical-align: middle;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
/*////////////////////////////////////////////*/
 +
 
 +
.com li{
 +
  line-height:200%;
 +
  vertical-align: middle;
 +
}
 +
 
 +
.underline{
 +
  display:block;
 +
  position:relative;
 +
  top:-10px;
 +
  left:0px;
 +
  border-bottom:4px dotted black;
 +
  width: 302px;
 +
}
 +
 
 +
.underline2{
 +
  display:block;
 +
  position:relative;
 +
  top:-10px;
 +
  left:0px;
 +
  border-bottom:4px dotted black;
 +
  width: 215px;
 +
}
 +
 
 +
.underline3{
 +
  display:block;
 +
  position:relative;
 +
  top:-10px;
 +
  left:0px;
 +
  border-bottom:4px dotted black;
 +
  width: 508px;
 +
}
 +
 
 +
.underline4{
 +
  display:block;
 +
  position:relative;
 +
  top:-10px;
 +
  left:0px;
 +
  border-bottom:4px dotted black;
 +
  width: 270px;
 +
}
 +
 
 +
.underline5{
 +
  display:block;
 +
  position:relative;
 +
  top:-10px;
 +
  left:0px;
 +
  border-bottom:4px dotted black;
 +
  width: 620px;
 +
}
 +
 
 +
.underline6{
 +
  display:block;
 +
  position:relative;
 +
  top:-10px;
 +
  left:0px;
 +
  border-bottom:4px dotted black;
 +
  width: 582px;
 +
}
 +
 
 +
#top_title{
 +
display:none;
 +
}
 +
 
 +
#sideMenu{
 +
display:none;
 +
position:fixed;
 +
right:120px;
 +
}
 +
 
 +
#content{
 +
  width:100%;
 +
  margin: 0px 0px 0px 0px;
 +
  padding: 0px 0px 0px 0px;
 +
}
 +
 
 +
.title{
 +
  font-size: 48.75pt;
 +
  margin-left:300px;
 +
  margin-top:112.5px;
 +
  cursor: default;
 +
}
 +
 
 +
.subtitle{
 +
  margin-left:12.25px;
 +
  margin-top:55px;
 +
  cursor: default;
 +
  font-size: 18pt;
 +
}
 +
 
 +
 
 +
.maintext{
 +
    font-size: 9pt;
 +
    font-family:Helvetica;
 +
    margin-top:10px;
 +
    text-align: justify;
 +
    cursor: default;
 +
    width:75%;
 +
}
 +
 
 +
.maintextsuite{
 +
    font-size: 9pt;
 +
    margin-top:10px;
 +
    text-align: justify;
 +
    cursor: default;
 +
    font-family:Helvetica;
 +
    width:75%;
 +
}
 +
 
 +
.maintextsuiteimage{
 +
    font-size: 9pt;
 +
    margin-top:10px;
 +
    text-align: justify;
 +
    cursor: default;
 +
    font-family:Helvetica;
 +
    width:40%;
 +
}
 +
 
 +
.text {
 +
  position:relative;
 +
  margin-left: 287.75px;
 +
  line-height:12pt;
 +
}
 +
 
 +
#logo{
 +
  font-size:18px;
 +
text-decoration:none;
 +
color:black;
 +
}
 +
 
 +
#logo:hover{
 +
  color: #3298CB;
 +
}
 +
 
 +
/*Flèche de remontée*/
 +
 
 +
#toparrow{
 +
    justify-content: center;
 +
    position:fixed;
 +
    vertical-align:middle;
 +
    z-index:10;
 +
    width:2%;
 +
    top:42%;
 +
    right:30px;
 +
}
 +
 
 +
#toparrow a:hover{
 +
  color: #3298CB;
 +
}
 +
 
 +
.Chili{
 +
  z-index:-1;
 +
  margin-left: 287.75px;
 +
  top:170px;
 +
  width:5vw;
 +
  height: 28vh;
 +
}
 +
 
 +
table{
 +
  border-collapse: collapse;
 +
  }
 +
 
 +
/*////////////////////////////////////////////*/
 +
/*Ensemble de class pour les onglets*/
 +
 
 +
.outer {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 28%;
 +
}
 +
 
 +
.outer1 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 38%;
 +
}
 +
 
 +
.outer1b {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 47.5%;
 +
}
 +
 
 +
.outer2 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 25%;
 +
}
 +
 
 +
.outer3 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 34%;
 +
}
 +
.outer3a {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 48%;
 +
}
 +
.outer3b {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 59%;
 +
}
 +
 
 +
.outer4 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 26%;
 +
}
 +
 
 +
.outer5 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 28%;
 +
}
 +
 
 +
.outer6 {
 +
  position: fixed;
 +
  left: 33px;
 +
  top: 42.5%;
 +
  text-align: center;
 +
}
 +
 
 +
.outer7 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 50.7%;
 +
}
 +
 
 +
.outer8 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 22%;
 +
}
 +
 
 +
.outer8b {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 31%;
 +
}
 +
 
 +
.outer9 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 42%;
 +
}
 +
 
 +
.outer10 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 49%;
 +
}
 +
 
 +
.outer11 {
 +
  position: fixed;
 +
  left: 33px;
 +
  top: 57%;
 +
  text-align: center;
 +
}
 +
 
 +
.outer12 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 22%;
 +
}
 +
 
 +
.outer13 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 28%;
 +
}
 +
 
 +
.outer13b {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 40%;
 +
}
 +
 
 +
.outer14 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 54%;
 +
}
 +
 
 +
.outer15 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 50%;
 +
}
 +
 
 +
.outer16 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 36%;
 +
}
 +
 
 +
.inner {
 +
  position: absolute;
 +
  bottom: 100%;
 +
  -webkit-transform: rotateZ(90deg);
 +
  transform-origin: 0 100%;
 +
  white-space: nowrap;
 +
}
 +
/*////////////////////////////////////////////*/
 +
 
 +
 
 +
.rotate {
 +
  -webkit-transform: rotate(180deg);
 +
}
 +
 
 +
#Team, #Design, #Project, #HumanPractice, #Achievements, #Labwork{
 +
  font-family: Helvetica, sans-serif;
 +
    font-weight: bold;
 +
    font-size: 8pt;
 +
}
 +
 
 +
/*Trait rouge des onglets*/
 +
 
 +
#batonbleu{
 +
    position:relative;
 +
    top:5px;
 +
    left:33%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu1{
 +
    position:relative;
 +
    top:5px;
 +
    left:33%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu1b{
 +
    position:relative;
 +
    top:5px;
 +
    left:33%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
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 +
 
 +
#batonbleu2{
 +
    position:relative;
 +
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 +
    width:22.5px;
 +
    background: #3298CB;
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    content:""
 +
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 +
 
 +
#batonbleu3{
 +
    position:relative;
 +
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 +
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 +
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 +
    background: #3298CB;
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    content:""
 +
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 +
#batonbleu3a{
 +
    position:relative;
 +
    top:5px;
 +
    left:33%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
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 +
#batonbleu3b{
 +
    position:relative;
 +
    top:5px;
 +
    left:15%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
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 +
 
 +
#batonbleu4{
 +
    position:relative;
 +
    top:5px;
 +
    left:33%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
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 +
 
 +
#batonbleu5{
 +
    position:relative;
 +
    top:5px;
 +
    left:33%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu6{
 +
    position:relative;
 +
    top:5px;
 +
    left:25%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu7{
 +
    position:relative;
 +
    top:5px;
 +
    left:22%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu8{
 +
    position:relative;
 +
    top:5px;
 +
    left:30%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu8b{
 +
    position:relative;
 +
    top:5px;
 +
    left:30%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu9{
 +
    position:relative;
 +
    top:5px;
 +
    left:29%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu10{
 +
    position:relative;
 +
    top:5px;
 +
    left:33%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu11{
 +
    position:relative;
 +
    top:5px;
 +
    left:25%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu12{
 +
    position:relative;
 +
    top:5px;
 +
    left:10%;
 +
    display:none;
 +
    height:3px;
 +
    width:17.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu13{
 +
    position:relative;
 +
    top:5px;
 +
    left:33%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
#batonbleu13b{
 +
    position:relative;
 +
    top:5px;
 +
    left:33%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu14{
 +
    position:relative;
 +
    top:5px;
 +
    left:37%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu15{
 +
    position:relative;
 +
    top:5px;
 +
    left:10%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
#batonbleu16{
 +
    position:relative;
 +
    top:5px;
 +
    left:35%;
 +
    display:none;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
li{margin:5px}
 +
 
 +
a:visited {
 +
    color: black;
 +
    text-decoration:none;
 +
}
 +
 
 +
 
 +
/*////////////////////////////////*/
 +
 
 +
 
 +
#title{
 +
  font-size: 60pt;
 +
  border-bottom:4px dotted black;
 +
  width: 182px;
 +
  z-index:-1;
 +
  margin-left: 287.75px;
 +
  margin-top: 90px;
 +
  line-height:59pt;
 +
}
 +
 
 +
#subtitles{
 +
  position:relative;
 +
  margin-left: 300px;
 +
  margin-top:-5vw;
 +
  font-size: 18pt;
 +
  font-weight: normal;
 +
  /*border-bottom:4px dotted black;*/
 +
  z-index:-1;
 +
  width: 120px;
 +
  border-bottom:4px dotted black;
 +
}
 +
 
 +
li{margin:5px}
 +
 
 +
/*////////////////////////////////////////////*/
 +
 
 +
</style>
 +
  </head>
 +
 
 +
 
 +
  <body>
 +
  <a class="left" href="https://2017.igem.org/Team:Pasteur_Paris"><img src="https://static.igem.org/mediawiki/2017/d/d8/T--Pasteur_Paris--aether.png"; width="220px"; height="70px"></img></a>
 +
  <a class="right" href="https://2017.igem.org/Team:Pasteur_Paris"><img src="https://static.igem.org/mediawiki/2017/1/10/T--Pasteur_Paris--iGEM.png"; width="90px"; height="70px"></img></a>
 +
<nav class="menu">
 +
  <ul>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionTeam()">Team </span></FONT></li>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionProject()">Project </span></FONT></li>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionDesign()">Design </span></FONT></li>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionLabWork()">Lab work </span></FONT></li>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionHumanPractice()">Human Practice </span></FONT></li>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionAchievements()">Achievements </span></FONT></li>
 +
  </ul>
 +
</nav>
 +
 
 +
<div id="Team" style="display:none">
 +
  <div class="outer">
 +
    <div class="inner"; onmouseover="apparitionbaton()"; onmouseout="deletebaton()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Team">Our Team </a>
 +
        <div id="batonbleu">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer1">
 +
    <div class="inner"; onmouseover="apparitionbaton1()"; onmouseout="deletebaton1()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Team#pagesponsors">Sponsors </a>
 +
        <div id="batonbleu1">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer1b">
 +
    <div class="inner"; onmouseover="apparitionbaton1b()"; onmouseout="deletebaton1b()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Attributions">Attributions </a>
 +
        <div id="batonbleu1b">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 
</div>
 
</div>
−
<div class="clear"></div>
 
  
 +
<div id="Project" style="display:none">
 +
  <div class="outer2">
 +
    <div class="inner"; onmouseover="apparitionbaton2()"; onmouseout="deletebaton2()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Project">Context</a>
 +
        <div id="batonbleu2">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer3">
 +
    <div class="inner"; onmouseover="apparitionbaton3()"; onmouseout="deletebaton3()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Entrepreneurship">Entrepreneurship</a>
 +
        <div id="batonbleu3">
 +
        </div>
 +
      </div>
 +
  </div>
 +
  </div>
 +
    <div class="outer3a">
 +
    <div class="inner"; onmouseover="apparitionbaton3a()"; onmouseout="deletebaton3a()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Engagement">Engagement</a>
 +
        <div id="batonbleu3a">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer3b">
 +
    <div class="inner"; onmouseover="apparitionbaton3b()"; onmouseout="deletebaton3b()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Model">Model</a>
 +
        <div id="batonbleu3b">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
  
−
<div class="column full_size">
+
<div id="Design" style="display:none">
−
<h1>Demonstrate</h1>
+
  <div class="outer5">
−
<h3>Gold Medal Criterion #4</h3>
+
    <div class="inner"; onmouseover="apparitionbaton5()"; onmouseout="deletebaton5()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Design">Design Methodology</a>
 +
        <div id="batonbleu5">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer6">
 +
    <div class="inner"; onmouseover="apparitionbaton6()"; onmouseout="deletebaton6()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Applied_Design">Applied<br>Design</a>
 +
        <div id="batonbleu6">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer7">
 +
    <div class="inner"; onmouseover="apparitionbaton7()"; onmouseout="deletebaton7()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Science">Science</a>
 +
        <div id="batonbleu7">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
  
−
<p>
+
<div id="Labwork" style="display:none">
−
Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
+
  <div class="outer8">
−
</p>
+
    <div class="inner"; onmouseover="apparitionbaton8()"; onmouseout="deletebaton8()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Notebook">Notebook</a>
 +
        <div id="batonbleu8">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer8b">
 +
    <div class="inner"; onmouseover="apparitionbaton8b()"; onmouseout="deletebaton8b()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Demonstrate">Demonstrate</a>
 +
        <div id="batonbleu8b">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer9">
 +
    <div class="inner"; onmouseover="apparitionbaton9()"; onmouseout="deletebaton9()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/InterLab">InterLab</a>
 +
        <div id="batonbleu9">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer10">
 +
    <div class="inner"; onmouseover="apparitionbaton10()"; onmouseout="deletebaton10()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Protocol">Protocol</a>
 +
        <div id="batonbleu10">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer11">
 +
    <div class="inner"; onmouseover="apparitionbaton11()"; onmouseout="deletebaton11()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Safety">Safety<br>Security</a>
 +
        <div id="batonbleu11">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
  
−
<p>
+
<div id="HumanPractice" style="display:none">
−
Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
+
  <div class="outer12">
−
</p>
+
    <div class="inner"; onmouseover="apparitionbaton12()"; onmouseout="deletebaton12()">
 +
      <div class="list rotate">
 +
      <a href="https://2017.igem.org/Team:Pasteur_Paris/HP/Law">Law</a>
 +
      <div id="batonbleu12"></div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer13">
 +
    <div class="inner"; onmouseover="apparitionbaton13()"; onmouseout="deletebaton13()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/HP/Collaborations">Collaborations</a>
 +
        <div id="batonbleu13">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
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        <div id="batonbleu14">
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        </div>
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 +
    </div>
 +
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 +
</div>
 +
 
 +
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        <div id="batonbleu16">
 +
        </div>
 +
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 +
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 +
  </div>
 +
</div>
  
  
 +
<div style="height:75vh">
 +
  <img src="https://static.igem.org/mediawiki/2017/1/16/T--Pasteur_Paris--Sci2.png"; style="position:absolute; left:390px; height: 66.5vh;width:auto;max-width: 60vw; z-index:-2;top:-70px"/>
 +
  <p id="title" style="font-size:50pt; font-family: MinionPro, serif;margin-top:100px">Demonstration</p>
 +
  <div class="Chili"></div>
 
</div>
 
</div>
  
 +
<div class="text">
 +
<div class="subtitle">
 +
  <p style="font-family: MinionPro, serif; font-size:18pt">Part I :Science</p>
 +
  <div class="underline"></div>
 +
</div>
  
−
<div class="column half_size">
+
<div class="subtitle">
 +
  <p style="font-family: MinionPro, serif;"><b>Proof of the efficiency of the degradation of Anthracene a Poly Aromatic hydrocarbon.</b></p>
 +
</div>
  
−
<h4> What should we do for our demonstration?</h4>
+
<div class="subtitle">
 +
  <p style="font-family: MinionPro, serif;font-size: 18pt">1. The molecule targeted: Anthracene</p>
 +
  <div class="underline"></div>
 +
</div>
  
−
<h5> Standard teams </h5>
+
<p class="maintext"> <b><span style="font-size:15pt; font-family: MinionPro">We </span> demonstrated the effective degradation of PAHs using anthracene as a representative molecule. It is possible to measure its concentration since anthracene absorbs at 254 nm, and therefore the kinetics of the reaction can be followed as well. We prepared our enzyme mix composed of E1(E1_1, E1_2), E2, E3, E4 by expression in <i>E. coli</i> (see below) (See Biobrick parts section for correspondence of enzyme names) </b></p>
  
−
<p>  
+
<div style="width:100% ; margin-top:20px;margin:auto; top: 70px">
−
If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
+
<div style="margin:auto ;width:100%">
−
</p>
+
  <img src="https://static.igem.org/mediawiki/2017/7/77/T--Pasteur_Paris--anthra.png"; style="height:auto; max-height:150%">
 +
  <p class="maintext" style="top: 30px; width:50vw; text-align: center"> Figure 1 : schema of anthracene</p>
 +
  <b class="maintext" style="top: 30px; width:50vw; text-align: center"> Anthracene was obtained in solution in 80% glycerol/water v/v at 0.1 mg/ml (Generous gift from Chimie ParisTech).</b>
 +
</div>
 
</div>
 
</div>
  
−
<div class="column half_size">
+
<div class="subtitle">
 +
  <p style="font-family: MinionPro, serif;font-size:18pt">2.  Preparation of the enzymes</p>
 +
  <div class="underline2"></div>
 +
</div>
 +
 
 +
<p class="maintext" style=" font-size:9pt">The five open reading frames coding for the four enzymes of the degradation pathway were independently expressed in BL21De3 cells. Cell pellets stored at -80°c, were thawed in lysis buffer (Tris-Cl 50 mM, pH 7.4, NaCl 100 mM, Glycerol 5% v/v) and sonicated. After centrifugation, supernatants were kept on ice for the enzymatic assay. (See Protein expression protocol).</p>
 +
 
 +
<div class="subtitle">
 +
  <p style="font-family: MinionPro, serif;font-size:18pt">3. Solutions tested :</p>
 +
  <div class="underline3"></div>
 +
</div>
 +
 
 +
<p class="maintext" style=" font-size:9pt">Buffers for enzymatic reaction:</p>
 +
 
 +
<div style="width:50% ;  margin-left:10%">
 +
<div style="width:100%">
 +
<table style="position:relative; left: 120px; max-width: 60%">
 +
    <tr>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Composition of the buffers for :</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Reaction volumes (μl): A</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">The blank volumes (μl): B</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Solution of trisphenylphosphine (5M)</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">13.2 μL (0.050M)</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">13.2 μL (0.050M)</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Solution of Anthracene</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">660 μL</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">0 μL</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Solution of NaCl (1M)</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">66 μL (0.050M)</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">66 μL (0.050M)</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Water</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">0 μL</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">660 μL</td>
 +
    </tr>
 +
</table></div></div>
 +
 
 +
 
 +
<p class="maintext" style=" font-size:9pt">The Reaction mixes:</p>
  
 +
<div style="width:50% ;  margin-left:10%">
 +
<div style="width:100%">
 +
<table style="position:relative; left: 120px; width: 60%">
 +
    <tr>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt"></td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Tests</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Blank</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Reaction buffer</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">67.2 μL of A</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">67.2 μL of B</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Enzyme</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">10 μL for each enzymes (50 μL when there are the five)</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">10 μL for each enzymes (50 μL when there are the five)</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Water</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Complementary to 120 μL as a total volume (which means 2.8 μL when there are the five enzymes)</td>
 +
      <td style="font-family: MinionPro, serif; font-size: 11pt">Complementary to 120 μL as a total volume (which means 2.8 μL when there are the five enzymes)</td>
 +
    </tr>
 +
</table></div></div>
 
<br>
 
<br>
−
<h5> Special track teams </h5>
+
<p class="maintext" style=" font-size:9pt">For the Controls :</p>
 +
<p class="maintext" style=" font-size:9pt">The enzymes volumes are substituted with extracts of cells transformed with pET32a only, and induced for the same length of time.</p>
  
−
<p>
+
 
−
Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
+
<div class="subtitle">
 +
  <p style="font-family: MinionPro, serif;font-size:18pt">4. Reactions kinetics</p>
 +
  <div class="underline4"></div>
 +
</div>
 +
 
 +
 
 +
<p class="maintext" style=" font-size:9pt"><b>An absorbance blank was performed using the enzyme mix in reaction buffer at 254 nm. This prevented the detection of DNA in the enzyme solution from the cell extracts. Anthracene was then added and a time point taken. Several other time points were taken after 3, 6, 12 and 24 minutes. The reaction was performed three times and the mean value of absorbance plotted against time to follow the kinetics. Error bars are shown as the standard deviation of the three measurements.</b></p>
 +
 
 +
<div style="width:50% ;  margin-left:10%">
 +
<div style="width:100%">
 +
  <img src="https://static.igem.org/mediawiki/2017/d/dc/T--Pasteur_Paris--A.png"; style="width:100%">
 +
</div>
 +
</div>
 +
 
 +
<div style="width:50% ;  margin-left:10%">
 +
<div style="width:100%">
 +
  <img src="https://static.igem.org/mediawiki/2017/7/7a/T--Pasteur_Paris--B.png"; style="width:100%">
 +
</div>
 +
</div>
 +
 
 +
<p class="maintext" style=" font-size:9pt"><b>On the Figure 5.A.: we can clearly see the concentration of anthracene decreasing in a constant and monotonous way over time. So, there is a degradation of the anthracene during the first 12 min and then the concentration stabilises itself.
 +
In the second graph Figure 5.B., we expected to see only a constant value. As it can be seen, except for point at time of 3 min, the concentration stays constant which means that there is no degradation without the catalysis.
 +
</b></p>
 +
 
 +
 
 +
<div class="subtitle">
 +
  <p style="font-family: MinionPro, serif;font-size:18pt">5. Conclusion</p>
 +
  <div class="underline5"></div>
 +
</div>
 +
 
 +
<p class="maintext" style=" font-size:9pt">We can conclude that our cocktail of four enzymes degrades effectively anthracene, a member of the PAH family, and therefore possibly other poly-aromatic compounds such as the benzo[a]pyrene.
 
</p>
 
</p>
  
 +
</div>
  
 +
<div class="text">
 +
<div class="subtitle">
 +
  <p style="font-family: MinionPro, serif; font-size:18pt">Part II :Design</p>
 +
  <div class="underline"></div>
 
</div>
 
</div>
  
 +
<div class="subtitle">
 +
  <p style="font-family: MinionPro, serif;font-size: 18pt"><b>Proof of the production of a particle filter system: Aether</b></p>
 +
</div>
  
 +
<p class="maintext" style=" font-size:9pt">Based on the ideation session, interviews with professionals, questionnaires to the public, and modelling of air flow, amount of enzymes and biopolymer to support it that can be possible etc ..  we designed and Air filter system to the scale that can be used in the household indoors. The device is equipped with a front cover air filter for large particles or fibres. It has a vinyl crafted surface for a prototype of Graetzel solar cells for its power supply. It also has holder for the bio-filter and charcoal filter units. It has a back cover to allow for air exit. And finally, it has a stand a strap system to either stand on a desk or set against a wall.</p>
  
 +
 +
<div style="width:50% ;  margin-left:10%">
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<div style="width:100%">
 +
  <img src="https://static.igem.org/mediawiki/2017/7/71/T--Pasteur_Paris--Dem3.jpeg"; style="width:100%"></img>
 +
    <p style=" font-family: Helvetica; text-align: justify;font-size: 9pt">Figure 3. The Aether system for air purification and degradation of poly aromatic hydrocarbons. </p>
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Latest revision as of 21:35, 15 December 2017

Demonstration

Part I :Science

Proof of the efficiency of the degradation of Anthracene a Poly Aromatic hydrocarbon.

1. The molecule targeted: Anthracene

We demonstrated the effective degradation of PAHs using anthracene as a representative molecule. It is possible to measure its concentration since anthracene absorbs at 254 nm, and therefore the kinetics of the reaction can be followed as well. We prepared our enzyme mix composed of E1(E1_1, E1_2), E2, E3, E4 by expression in E. coli (see below) (See Biobrick parts section for correspondence of enzyme names)

Figure 1 : schema of anthracene

Anthracene was obtained in solution in 80% glycerol/water v/v at 0.1 mg/ml (Generous gift from Chimie ParisTech).

2. Preparation of the enzymes

The five open reading frames coding for the four enzymes of the degradation pathway were independently expressed in BL21De3 cells. Cell pellets stored at -80°c, were thawed in lysis buffer (Tris-Cl 50 mM, pH 7.4, NaCl 100 mM, Glycerol 5% v/v) and sonicated. After centrifugation, supernatants were kept on ice for the enzymatic assay. (See Protein expression protocol).

3. Solutions tested :

Buffers for enzymatic reaction:

Composition of the buffers for : Reaction volumes (μl): A The blank volumes (μl): B
Solution of trisphenylphosphine (5M) 13.2 μL (0.050M) 13.2 μL (0.050M)
Solution of Anthracene 660 μL 0 μL
Solution of NaCl (1M) 66 μL (0.050M) 66 μL (0.050M)
Water 0 μL 660 μL

The Reaction mixes:

Tests Blank
Reaction buffer 67.2 μL of A 67.2 μL of B
Enzyme 10 μL for each enzymes (50 μL when there are the five) 10 μL for each enzymes (50 μL when there are the five)
Water Complementary to 120 μL as a total volume (which means 2.8 μL when there are the five enzymes) Complementary to 120 μL as a total volume (which means 2.8 μL when there are the five enzymes)

For the Controls :

The enzymes volumes are substituted with extracts of cells transformed with pET32a only, and induced for the same length of time.

4. Reactions kinetics

An absorbance blank was performed using the enzyme mix in reaction buffer at 254 nm. This prevented the detection of DNA in the enzyme solution from the cell extracts. Anthracene was then added and a time point taken. Several other time points were taken after 3, 6, 12 and 24 minutes. The reaction was performed three times and the mean value of absorbance plotted against time to follow the kinetics. Error bars are shown as the standard deviation of the three measurements.

On the Figure 5.A.: we can clearly see the concentration of anthracene decreasing in a constant and monotonous way over time. So, there is a degradation of the anthracene during the first 12 min and then the concentration stabilises itself. In the second graph Figure 5.B., we expected to see only a constant value. As it can be seen, except for point at time of 3 min, the concentration stays constant which means that there is no degradation without the catalysis.

5. Conclusion

We can conclude that our cocktail of four enzymes degrades effectively anthracene, a member of the PAH family, and therefore possibly other poly-aromatic compounds such as the benzo[a]pyrene.

Part II :Design

Proof of the production of a particle filter system: Aether

Based on the ideation session, interviews with professionals, questionnaires to the public, and modelling of air flow, amount of enzymes and biopolymer to support it that can be possible etc .. we designed and Air filter system to the scale that can be used in the household indoors. The device is equipped with a front cover air filter for large particles or fibres. It has a vinyl crafted surface for a prototype of Graetzel solar cells for its power supply. It also has holder for the bio-filter and charcoal filter units. It has a back cover to allow for air exit. And finally, it has a stand a strap system to either stand on a desk or set against a wall.

Figure 3. The Aether system for air purification and degradation of poly aromatic hydrocarbons.