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background-image:url('https://static.igem.org/mediawiki/2017/2/24/T--NYMU-Taipei--model_background.png'); | background-image:url('https://static.igem.org/mediawiki/2017/2/24/T--NYMU-Taipei--model_background.png'); | ||
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− | + | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | |
− | + | R: co2 productive rate | |
− | + | <br>Rmax: maximum rate mol/g*min //0.000046 | |
− | + | <br>i: irradiance uE/m^2 | |
− | + | <br>n: irradiance exponential constant//1.19 | |
− | + | <br>ki: productive coefficient uE/(m^2)*s //174 | |
− | + | <br>m: constant (m^2)*s /uE//0.0022 | |
− | + | </p> | |
− | + | </blockquote> | |
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R=A1exp(-E1/rT)-A2exp(-E2/rT) | R=A1exp(-E1/rT)-A2exp(-E2/rT) | ||
</h6> | </h6> | ||
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− | + | <blockquote> | |
− | + | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | |
− | + | R: co2 productive rate | |
− | + | <br>A1:preexponential factor at i=400 //1147.7 | |
− | + | <br>A2:preexponential factor at i=200 //3.818*10^8 | |
− | + | <br>E1:activation energy at i=400 mol/J //42700 | |
− | + | <br>E2:activation energy at i=200 mol/J //77100 | |
− | + | <br>T:temperature K | |
− | + | </p> | |
− | + | </blockquote> | |
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<br>Umax = A*exp(-E/RT) | <br>Umax = A*exp(-E/RT) | ||
</h6> | </h6> | ||
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+ | <blockquote> | ||
+ | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | ||
+ | U: specific growth rate day^-1 | ||
+ | <br>Umax: maximum specific growth rate day^-1 | ||
+ | <br>Kss: substrate parameter //1 | ||
+ | <br>A: constant day^-1 //1.0114*10^10 | ||
+ | <br>E: activation energy cal/mol//6842 | ||
+ | <br>R: gas constant cal/K*mol //8.314 | ||
+ | </p> | ||
+ | </blockquote> | ||
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− | + | <blockquote> | |
− | + | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | |
− | + | R: Co2 productive rate | |
− | + | <br>A1: preexponential factor at i=400 //8.625*10^-5 | |
− | + | <br>A2: preexponential factor at i=200 //1.83885*10^-2 | |
− | + | <br>B1: activation energy at i=400 mol/J //6.45 | |
− | + | <br>B2: activation energy at i=200 mol/J //69.2 | |
− | + | </p> | |
− | + | </blockquote> | |
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− | + | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | |
− | + | Rmax: maximum rate mol/g*min //0.000046 | |
− | + | <br>e: absorbed energy w/m^2 | |
− | + | <br>n: energy exponential constant//1.252 | |
− | + | <br>ke: productive coefficient uE/(m^2)*s //157.88 | |
− | + | <br>m: constant (m^2)*s /uE//0.0035 | |
− | + | </p> | |
− | + | </blockquote> | |
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<h6 style='color:#bc0101; font-family:"Roboto Mono", monospace;'>ln(Xt/X0)/t=A+Bexp(-C(t-M))=μ(specific growth rate)</h6> | <h6 style='color:#bc0101; font-family:"Roboto Mono", monospace;'>ln(Xt/X0)/t=A+Bexp(-C(t-M))=μ(specific growth rate)</h6> | ||
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− | + | <blockquote> | |
− | + | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | |
− | + | X: biomass concentration(g/l) | |
− | + | <br>t: time(hr) | |
− | + | <br>A: the asymptotic of ln Xt/Xo as t decrese indefinitely | |
− | + | <br>B: the asymptotic of ln Xt/Xo as t increase indefinitely | |
− | + | <br>C: the relative growth rate at time M | |
− | + | </p> | |
− | + | </blockquote> | |
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− | + | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | |
− | + | P: lipid | |
− | + | <br>N: nitrogen | |
− | + | <br>X: biomass | |
− | + | <br> | |
− | + | <br>α: the instantaneous yield coefficient of product formation due to cell growth | |
− | + | <br>β: the specific formation rate of product | |
− | + | <br> | |
− | + | <br>q: Minimum N quota | |
− | + | <br>qM: Maximum N quota | |
− | + | <br>Q: N quota | |
− | + | <br>Vm: Maximum uptake rate of nitrogen | |
− | + | <br>Vh: Half-saturation coefficient | |
− | + | </p> | |
− | + | </blockquote> | |
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− | + | <blockquote> | |
− | + | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | |
− | + | n1: biomass at frist state | |
− | + | <br>n2: biomass at secind state | |
− | + | <br>x: biomass concentration(g/l) | |
− | + | <br>t: time(hr) | |
− | + | <br> | |
− | + | <br>A: the asymptotic of ln Xt/Xo as t decrese indefinitely //-39.9532 | |
− | + | <br>B: the asymptotic of ln Xt/Xo as t increase indefinitely //-0.0222 | |
− | + | <br>C: the relative growth rate at time M hr //45.6931 | |
− | + | <br> | |
− | + | <br>k: constant //8.15229 | |
− | + | <br>b:yield coefficient//1207.569 | |
− | + | <br>ns:initial nitrogen concentration | |
− | + | <br>a:regression constant//0.01 | |
− | + | <br>e:a perturbation//0.50678 | |
− | + | </p> | |
− | + | </blockquote> | |
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dn/dt=Yxn*dx/dt+m*x | dn/dt=Yxn*dx/dt+m*x | ||
</h6> | </h6> | ||
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− | + | <blockquote> | |
− | + | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | |
− | + | n: nitrogen concentration | |
− | + | <br>Yxn: nitrate coefficient g/g 0.21016 | |
− | + | <br>m: maintenance parameter hr^-1 0.0014393 | |
− | + | <br>x: biomass concentration | |
− | + | </p> | |
− | + | </blockquote> | |
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− | + | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | |
− | + | p: lipid concentrtion | |
− | + | <br>K1: growth correlation coefficient g^2/g^2 //122.40085 | |
− | + | <br>K2: non-growth correlation coefficient g^-1 //0.28736 | |
− | + | <br>e: a perturbation g/l*hr //-0.078 | |
− | + | </p> | |
− | + | </blockquote> | |
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− | + | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | |
− | + | X: chlorella vugaris | |
− | + | <br>Z: e.coil | |
− | + | <br>Rx: symbiosis coefficient g/hr //1.0000023 | |
− | + | <br>Rz: symbiosis coefficient g/hr //1.178 | |
− | + | <br>Yx: correlation coefficient//12.576 | |
− | + | <br>Yz: correlation coefficient//2.276 | |
− | + | <br>a: population constant //0.80467 | |
− | + | <br>c: population constant//0.61198 | |
− | + | <br>b: relative parameter //0.00027 | |
− | + | <br>g: relative parameter //0.0013 | |
− | + | </p> | |
− | + | </blockquote> | |
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− | + | <p style='color:#702828;font-size:16px; font-family:"Roboto Mono", monospace;'> | |
− | + | l: lipid proportion in cell | |
− | + | <br>k: constant g/100g //1.13372 | |
− | + | <br>b: yield coefficient//1.57172 | |
− | + | <br>ns: initial nitrogen concentration | |
− | + | <br>a: regression constant//0.51653 | |
− | + | <br>e: a perturbation g/100g//-55.2776 | |
− | + | </p> | |
− | + | </blockquote> | |
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Revision as of 03:12, 15 October 2017
Modeling is an extremely important part to our project, because it helps us accurately check and predict the results of the experiments, which are worked in the wet lab. In our project, there are two essential types of microalgae that play very important roles, Synechosistic PCC7942andChlorella vulgaris. The following will show our success in modeling.
Synechosistis PCC7942
The modeling from figure 1 to figure 5 belongs to the experiments of Synechosistis PCC7942 pigments.
Chlorella vulgaris
The modeling from figure 6 to figure 12 belongs to the experiments of Chlorella vulgaris nitrogen starvation.
Modeling is an extremely important part to our project, because it helps us accurately check and predict the results of the experiments, which are worked in the wet lab. In our project, there are two essential types of microalgae that play very important roles, Synechosistic PCC7942andChlorella vulgaris. The following will show our success in modeling.
Synechosistis PCC7942
The modeling from figure 1 to figure 5 belongs to the experiments of Synechosistis PCC7942 pigments.
Chlorella vulgaris
The modeling from figure 6 to figure 12 belongs to the experiments of Chlorella vulgaris nitrogen starvation.