Difference between revisions of "Team:TU Darmstadt/project/chitin synthase"

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<p>The goal of our project is the production of a chitosan hydrogel which uses chitin as source material. This <i>N</i>-acetylglucosamine oligosaccharide (chitin) can be produced in <i>E. coli</i> via a Chitin Synthase (CHS). The enzyme used here is CHS NodC from the bacteria <i>Rhizobium Leguminosarum</i>. NodC is an <i>N</i>-acetylglucosaminyl transferase which builds chitin tetramers and pentamers using activated <i>N</i>-Acetylglucosamine monomers <i>[Samain et al., 1997]</i>. Originally, chitin is chemically recovered from crustacean shells, which produces a lot of waste and chitin oligosaccharides of unspecified length <i>[Kurita, 2006]</i>. To avoid this waste, we produce chitin oligosaccharides in <i>E. coli</i> by insertion of the nodC gene with the BioBrick system <i>[Knight et al., 2003]</i>. NodC reliably produces short oligosaccharides of certain lengths which can be directly further processed <i>in vivo</i>.<i>[Samain et al., 1997]</i></p>
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<p>The goal of our project is the production of a chitosan hydrogel which uses chitin as source material. This <i>N</i>-acetylglucosamine oligosaccharide (chitin) can be produced in <i>E. coli</i> via a Chitin Synthase (CHS). The enzyme used here is CHS NodC from the bacteria <i>Rhizobium Leguminosarum</i>. NodC is an <i>N</i>-acetylglucosaminyl transferase which builds chitin tetramers and pentamers using activated <i>N</i>-Acetylglucosamine monomers <i>[Samain et al., 1997]</i>. Originally, chitin is chemically recovered from crustacean shells, which uses a lot of chemicals and produces chitin oligosaccharides of unspecified length <i>[Kurita, 2006]</i>. To avoid this chemical process, we produce chitin oligosaccharides in <i>E. coli</i> by insertion of the <i>nodC</i> gene with the BioBrick system <i>[Knight et al., 2003]</i>. NodC reliably produces short oligosaccharides of certain lengths which can be directly further processed <i>in vivo</i>.<i>[Samain et al., 1997]</i></p>
 
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Revision as of 18:28, 13 October 2017

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Chitin Synthase NodC

Abstract

The goal of our project is the production of a chitosan hydrogel which uses chitin as source material. This N-acetylglucosamine oligosaccharide (chitin) can be produced in E. coli via a Chitin Synthase (CHS). The enzyme used here is CHS NodC from the bacteria Rhizobium Leguminosarum. NodC is an N-acetylglucosaminyl transferase which builds chitin tetramers and pentamers using activated N-Acetylglucosamine monomers [Samain et al., 1997]. Originally, chitin is chemically recovered from crustacean shells, which uses a lot of chemicals and produces chitin oligosaccharides of unspecified length [Kurita, 2006]. To avoid this chemical process, we produce chitin oligosaccharides in E. coli by insertion of the nodC gene with the BioBrick system [Knight et al., 2003]. NodC reliably produces short oligosaccharides of certain lengths which can be directly further processed in vivo.[Samain et al., 1997]

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