Difference between revisions of "Team:East Chapel Hill/Demonstrate"

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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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<h4> What should we do for our demonstration?</h4>
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<h5> Standard teams </h5>
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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          <img src="https://static.igem.org/mediawiki/2017/6/65/T--East_Chapel_Hill--Icon-new.png">
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        <li><a href="https://2017.igem.org/Team:East_Chapel_Hill">Home</a></li>
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        <a class="dropdown-toggle" data-toggle="dropdown" href="https://2017.igem.org/Team:East_Chapel_Hill/Team">Team
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Team">Members</a></li>
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        <a class="dropdown-toggle" data-toggle="dropdown" href="https://2017.igem.org/Team:East_Chapel_Hill/Designs">Project
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Designs">Design and Method</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Notebook">Lab Notebook</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Safety">Safety</a></li>
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        <li class=" active"><a href="https://2017.igem.org/Team:East_Chapel_Hill/results">Results</a></li>
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<li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Silver">Human Practices </a></li>
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        <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Collaborations">Collaborations</a></li>
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        <a class="dropdown-toggle" data-toggle="dropdown" href="https://2017.igem.org/Team:East_Chapel_Hill/Awards">Awards
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Awards">Achievements</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Demonstrate">Demonstrate</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Engagement">Engagement</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Measurement">Measurement</a></li>
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<h2 style="text-align:left;"> Demonstrate</h2>
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<h5> Special track teams </h5>
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Our project pertains to measuring concentrations of fluoride and characterizing fluoride riboswitches with higher affinity to fluoride; these technologies can be used to determine methods to sequester, bioremediate, and detect fluoride.<br>
  
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We developed the Chloramphenicol Acetyltransferase Operon (CHOP) regulated by the fluoride riboswitch as a system to characterize the fluoride riboswitch. <br>
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
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Since bacteria does not grow in the presence of chloramphenicol, the protein bacteria needs chloramphenicol acetyltransferase in order to build resistance to chloramphenicol and survive.
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We plated CHOP and ΔcrcB, which is our control E.coli without the resistance to chloramphenicol, onto plates with no fluoride and increased concentrations of fluoride in order to test the best level the fluoride riboswitch can grow at. <br>
  
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Our results demonstrated that with no fluoride there was no growth of CHOP. However, the growth of CHOP increased as the concentration of fluoride increased. In other words, CHOP is fluoride dependent. Our fluoride riboswitch was able to regulate the chloramphenicol acetyltransferase and allow the bacteria to survive; the concentration with the most growth of CHOP would represent the best activation concentration of fluoride for the riboswitch. <br>
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Since the growth of CHOP is correlated with the activation of the fluoride riboswitch, CHOP can be used to find other riboswitches that have a better affinity to fluoride by finding the lowest level of fluoride CHOP can grow at specific riboswitches.<br><br>
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Revision as of 06:40, 1 November 2017

Demonstrate


Our project pertains to measuring concentrations of fluoride and characterizing fluoride riboswitches with higher affinity to fluoride; these technologies can be used to determine methods to sequester, bioremediate, and detect fluoride.
We developed the Chloramphenicol Acetyltransferase Operon (CHOP) regulated by the fluoride riboswitch as a system to characterize the fluoride riboswitch.
Since bacteria does not grow in the presence of chloramphenicol, the protein bacteria needs chloramphenicol acetyltransferase in order to build resistance to chloramphenicol and survive. We plated CHOP and ΔcrcB, which is our control E.coli without the resistance to chloramphenicol, onto plates with no fluoride and increased concentrations of fluoride in order to test the best level the fluoride riboswitch can grow at.

Our results demonstrated that with no fluoride there was no growth of CHOP. However, the growth of CHOP increased as the concentration of fluoride increased. In other words, CHOP is fluoride dependent. Our fluoride riboswitch was able to regulate the chloramphenicol acetyltransferase and allow the bacteria to survive; the concentration with the most growth of CHOP would represent the best activation concentration of fluoride for the riboswitch.
Since the growth of CHOP is correlated with the activation of the fluoride riboswitch, CHOP can be used to find other riboswitches that have a better affinity to fluoride by finding the lowest level of fluoride CHOP can grow at specific riboswitches.