Difference between revisions of "Team:USTC/Safety"
| Line 280: | Line 280: | ||
<br> | <br> | ||
<p>Our project was designed to be a higher efficient synthesis platform with engineered bacterial applied in factories. There was nothing harmful to the public or environment. However, H2S and CdS, potential products in our photosynthesis system, might have been a risk for us, the researchers and we solved the problem successfully with the help of our universities and the SRB Safety Handbook of the team UNITN-Trento in 2012. | <p>Our project was designed to be a higher efficient synthesis platform with engineered bacterial applied in factories. There was nothing harmful to the public or environment. However, H2S and CdS, potential products in our photosynthesis system, might have been a risk for us, the researchers and we solved the problem successfully with the help of our universities and the SRB Safety Handbook of the team UNITN-Trento in 2012. | ||
| − | <p/><br> | + | <p/><br>Bacterial strains<br>We worked with non-pathogenic E.coli strains---Top 10 to amplify our plasmids and BL21(DE3) to express our target proteins in most experiments. To find out whether CdS nanoparticles can generate electrons or not, we used a bacteria called Shewanella oneidensis MR-1, which are non-pathogenic and well-characterized.<br>Parts<br>None of the new parts will raise safety issues for further applications except for the parts with the CysDes (BBa_K2242233, BBa_K2242633). We realized these parts would produce hydrogen sulfide at a very low concentration after induced from the wiki of team UNITN-Trento in 2012 at the beginning of our research. We stressed H2S issue again on the main page of the Parts that we submitted. When we did related research about Cysteine Desulfhydrase in our photosynthesis system.<br>H2S<br>We knew that H2S, a toxic substance, would be produced at a very low concentrations when Cysteine Desulfhydrase, an important enzyme in our photosynthesis system, was induced. Before experiments started, we read the wiki of the team UNITN-Trento in 2012 and other references to find a solution. We did all the experiments involved in the bacteria producing H2S in a specific hoods. We used a separate waste container to dispose of bacteria producing H2S. Eventually, these media and cultures were properly disposed in terms of the way mentioned in our “Protection Guide of lab work”.<br>As for further application in factories, the problem of tiny amounts of H2S releasing is easy to solve with well ventilation system and respiratory, skin and eye protection provided.<br>CdS<br>Nano CdS can be toxic if not handled correctly. In our experiments, we recovered nano CdS by means of centrifugation and encapsulated it to ensure no direct interaction with environment. Ultimately, the hazardous waste were collected by our schools and disposed properly.<br>When our project is performed in factories in future, nano CdS can be recovered properly and then applied in other area such as semiconductor. |
| + | |||
| + | |||
</div> | </div> | ||
<div > | <div > | ||
| Line 306: | Line 308: | ||
<div> | <div> | ||
<ul class="section table-of-contents"> | <ul class="section table-of-contents"> | ||
| − | <li><a href="#first"> | + | <li><a href="#first">Project safety</a></li> |
<li><a href="#second">Silver</a></li> | <li><a href="#second">Silver</a></li> | ||
<li><a href="#third">Gold</a></li> | <li><a href="#third">Gold</a></li> | ||
Revision as of 08:07, 23 October 2017
Project safety
Our project was designed to be a higher efficient synthesis platform with engineered bacterial applied in factories. There was nothing harmful to the public or environment. However, H2S and CdS, potential products in our photosynthesis system, might have been a risk for us, the researchers and we solved the problem successfully with the help of our universities and the SRB Safety Handbook of the team UNITN-Trento in 2012.
Bacterial strains
We worked with non-pathogenic E.coli strains---Top 10 to amplify our plasmids and BL21(DE3) to express our target proteins in most experiments. To find out whether CdS nanoparticles can generate electrons or not, we used a bacteria called Shewanella oneidensis MR-1, which are non-pathogenic and well-characterized.
Parts
None of the new parts will raise safety issues for further applications except for the parts with the CysDes (BBa_K2242233, BBa_K2242633). We realized these parts would produce hydrogen sulfide at a very low concentration after induced from the wiki of team UNITN-Trento in 2012 at the beginning of our research. We stressed H2S issue again on the main page of the Parts that we submitted. When we did related research about Cysteine Desulfhydrase in our photosynthesis system.
H2S
We knew that H2S, a toxic substance, would be produced at a very low concentrations when Cysteine Desulfhydrase, an important enzyme in our photosynthesis system, was induced. Before experiments started, we read the wiki of the team UNITN-Trento in 2012 and other references to find a solution. We did all the experiments involved in the bacteria producing H2S in a specific hoods. We used a separate waste container to dispose of bacteria producing H2S. Eventually, these media and cultures were properly disposed in terms of the way mentioned in our “Protection Guide of lab work”.
As for further application in factories, the problem of tiny amounts of H2S releasing is easy to solve with well ventilation system and respiratory, skin and eye protection provided.
CdS
Nano CdS can be toxic if not handled correctly. In our experiments, we recovered nano CdS by means of centrifugation and encapsulated it to ensure no direct interaction with environment. Ultimately, the hazardous waste were collected by our schools and disposed properly.
When our project is performed in factories in future, nano CdS can be recovered properly and then applied in other area such as semiconductor.
Silver
Gold
