Difference between revisions of "Team:USTC/Basic Part"

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                                                     <td>Cysteine Desulfhydrase is an aminotransferase that converts cysteine into pyruvate, ammonia, and hydrogen sulfide. In our project, we use it to generate Cd<sup>2+</sup></td>
 
                                                     <td>Cysteine Desulfhydrase is an aminotransferase that converts cysteine into pyruvate, ammonia, and hydrogen sulfide. In our project, we use it to generate Cd<sup>2+</sup></td>
 
                                                     <td>Synthesized by IDT.</td>
 
                                                     <td>Synthesized by IDT.</td>
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                                                     <td>As this aminotransferase can produce hydrogen sulfide when it’s functioning, so we must emphasize more attention to the safety problem when we are using this part. In addition, we use this enzyme to generate CdS nanoparticles, which is also kind of an polluted chemical. So we take a few measures to take care this pollutants.</td>
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                                                     <td>As this aminotransferase can produce hydrogen sulfide and CdS nanoparticles if S<sup>2-</sup>is in the system, special measures must be taken.</td>
 
                                                   </tr>
 
                                                   </tr>
 
                                                   <tr>
 
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Revision as of 14:53, 23 October 2017

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This year we, team USTC, submitted 5 basic parts. All of them are coding part as we did not use any special promoters or RBS in our project. Among these five basic parts, two of them are new, including reductase CmCR and KmAdh.

Part’s Number Part’s Name Type Length Short Description Source Safety Issues
BBa_K2242363 Mtr CAB Coding 5210 It consists of 3 proteins, Mtr A, Mtr B and Mtr C, which plays the role as electron tunnel to transfer extracellular electrons into the cytoplasm. It comes from the kit plate from iGEM 2017.
BBa_K2242666 Ccm A-H Coding 6290 Its full name is Cytochrome c Maturation, which can mature Mtr CAB and enable it to function as we expected. We cloned this gene ccm A-H from the genome of an E.coli strain BL21(DE3).
BBa_K2242233 CysDes Coding 1203 Cysteine Desulfhydrase is an aminotransferase that converts cysteine into pyruvate, ammonia, and hydrogen sulfide. In our project, we use it to generate Cd2+ Synthesized by IDT. As this aminotransferase can produce hydrogen sulfide and CdS nanoparticles if S2-is in the system, special measures must be taken.
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