Difference between revisions of "Team:Stuttgart/Notebook"

Line 1: Line 1:
 
<html>
 
<html>
  
−
<script>
+
<!--- THIS IS WHERE THE HTML BEGINS --->
  
−
     // This is the jquery part of your template.
+
<head>
−
    // Try not modify any of this code too much since it makes your menu work.
+
     <link href="https://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet"
 +
          id="bootstrap-css">
 +
    <style>
  
−
    $(document).ready(function() {
 
  
−
         $("#HQ_page").attr('id','');
+
         /***************************************************** DEFAULT WIKI SETTINGS  ****************************************************/
  
−
         // call the functions that control the menu
+
         /* Clear the default wiki settings */
−
        menu_functionality();
+
−
        hide_show_menu();
+
  
 +
        #home_logo, #sideMenu {
 +
            display: none;
 +
        }
  
 +
        #sideMenu, #top_title, .patrollink {
 +
            display: none;
 +
        }
  
−
         //this function controls the expand and collapse buttons of the menu and changes the +/- symbols
+
         #content {
−
         function menu_functionality() {
+
            width: 100%;
 +
            padding: 0px;
 +
            margin-top: -7px;
 +
            margin-left: 0px;
 +
         }
  
−
            //when clicking on a "menu_button", it will change the "+/-" accordingly and it will show/hide the corresponding submenu
+
        body {
−
             $(".menu_button").click(function(){
+
             background: white;
 +
        }
  
−
                // add or remove the class "open" , this class holds the "-"
+
        #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {
−
                $(this).children().toggleClass("open");
+
            margin-bottom: 0;
−
                // show or hide the submenu
+
            color: #F8CE63;
−
                $(this).next('.submenu_wrapper').fadeToggle(400);
+
        }
−
            });
+
  
−
            // when the screen size is smaller than 800px, the display_menu_control button appears and will show/hide the whole menu
+
        table {;
−
            $("#display_menu_control").click(function(){
+
        }
−
                $('#menu_content').fadeToggle(400);
+
−
            });
+
  
−
            // call the current page highlight function
+
        /**************************************************************** MENU ***************************************************************/
−
             highlight_current_page();
+
        /* Wrapper for the menu */
 +
        .igem_2017_menu_wrapper {
 +
            width: 15%;
 +
            height: 100vh;
 +
            position: fixed;
 +
            right: 0;
 +
            padding: 0;
 +
            float: right;
 +
            border-left: 1px solid #F8CE63;
 +
            background-color: white;
 +
            text-align: left;
 +
            font-family: Tahoma, Geneva, sans-serif;
 +
            overflow-y: auto;
 +
             overflow-x: hidden;
 
         }
 
         }
  
 +
        /* this hides the scrollbar to keep view consistency */
 +
        .igem_2017_menu_wrappe::-webkit-scrollbar {
 +
            display: none;
 +
        }
  
−
         // call the highlight current page function to show it on the menu with a different background color
+
         /* styling for links in the menu, removes the line under text */
−
         function highlight_current_page() {
+
         .igem_2017_menu_wrapper a {
 +
            text-decoration: none;
 +
        }
  
−
            // select a page from the menu based on the id assigned to it and the current page name and add the class "current page" to make it change background color
+
        /* styling for the images in the menu */
−
             $("#"+  wgPageName.substring(wgPageName.lastIndexOf("/")+1, wgPageName.length ) + "_page").addClass("current_page");
+
        .igem_2017_menu_wrapper img {
 +
             width: 100%;
 +
        }
  
−
            // now that the current_page class has been added to a menu item, make the submenu fade in
+
        /* styling for the menu buttons */
−
            $(".current_page").parents(".submenu_wrapper").fadeIn(400);
+
        .igem_2017_menu_wrapper .menu_button {
−
             // change the +/- symbol of the corresponding menu button
+
            width: 100%;
−
             $(".current_page").parents(".submenu_wrapper").prev().children().toggleClass("open");
+
             padding: 10px 0px 10px 15px;
 +
            float: left;
 +
            border-bottom: 1px solid #F8CE63;
 +
             font-size: 16px;
 +
            font-weight: bold;
 +
            color: #F8CE63;
 +
            cursor: pointer;
 +
        }
  
 +
        .igem_2017_menu_wrapper .menu_bottom_padding {
 +
            width: 100%;
 +
            height: 30px;
 +
            float: left;
 
         }
 
         }
  
 +
        .menu_button.direct_to_page {
 +
            padding-left: 36px;
 +
        }
  
 +
        .igem_2017_menu_wrapper .menu_button .expand_collapse_icon {
 +
            width: 10%;
 +
            float: left;
 +
        }
  
−
         // allow button on the black menu bar to show/hide the side menu
+
         .igem_2017_menu_wrapper .menu_button .expand_collapse_icon::before {
−
         function hide_show_menu() {
+
            content: "+";
 +
         }
  
−
            // in case you preview mode is selected, the menu is hidden for better visibility
+
        /* styling for the menu buttons on hover */
−
            if (window.location.href.indexOf("submit") >= 0) {
+
        .igem_2017_menu_wrapper .menu_button:hover, .igem_2017_menu_wrapper .submenu_button:hover, .submenu_button.current_page:hover {
−
                $(".igem_2017_menu_wrapper").hide();
+
            background-color: #F8CE63;
−
             }
+
             text-decoration: none;
 +
            color: #190707;
 +
        }
  
−
            // if the black menu bar has been loaded
+
        /* styling for the menu button when it is the current page */
−
            if (document.getElementById('bars_item')) {
+
        .current_page {
 +
            background-color: white !important;
 +
            color: #F8CE63 !important;
 +
        }
  
−
                // when the "bars_item" has been clicked
+
        /* styling for the submenu buttons */
−
                $("#bars_item").click(function() {
+
        .igem_2017_menu_wrapper .submenu_button {
−
                    $("#sideMenu").hide();
+
            width: 100%;
 +
            padding: 10px 0px 10px 34px;
 +
            float: left;
 +
            background-color: white;
 +
            border-bottom: 1px solid #F8CE63;
 +
            font-size: 15px;
 +
            color: #F8CE63;
 +
            cursor: pointer;
 +
        }
  
−
                    // show/hide the menu wrapper
+
        /* wrapper for the submenu items, they are hidden by default*/
−
                    $(".igem_2017_menu_wrapper").fadeToggle("100");
+
        .igem_2017_menu_wrapper .submenu_wrapper {
−
                });
+
            display: none;
−
            }
+
        }
  
−
            // because the black menu bars loads at a different time than the rest of the page, this function is set on a time out so it can run again in case it has not been loaded yet
+
        /* when the page size is bigger than 800px, this show/hide control is hidden by default */
−
            else {
+
        .igem_2017_menu_wrapper #display_menu_control {
−
                setTimeout(hide_show_menu, 15);
+
            display: none;
−
             }
+
             text-align: center;
 
         }
 
         }
  
 +
        /***************************************************** CONTENT OF THE PAGE ****************************************************/
  
−
    });
+
        /* Wrapper for the content */
 +
        .igem_2017_content_wrapper {
 +
            width: 83%;
 +
            margin: 0;
 +
            display: block;
 +
            float: left;
 +
            background-color: white;
 +
            font-family: Tahoma, Geneva, sans-serif;
 +
        }
  
 +
        /********************************* HTML STYLING  *********************************/
  
−
</script>
+
        /* styling for the titles h1 h2 */
 +
        .igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 {
 +
            color: #F8CE63;
 +
            padding: 15px 0 25px 0;
 +
            border-bottom: 0;
 +
        }
  
−
<style>
+
        /* styling for the titles  h3 h4 h5 h6*/
 +
        .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 {
 +
            padding: 5px 15px;
 +
            border-bottom: 0;
 +
        }
  
 +
        /* font and text */
 +
        .container p,
 +
        #HQ_page p {
 +
            line-height: 24px;
 +
            font-size: 16px;
 +
            color: #333;
 +
        }
  
 +
        .igem_2017_content_wrapper img {
 +
            padding: 15px 0;
 +
        }
  
−
    /***************************************************** DEFAULT WIKI SETTINGS  ****************************************************/
+
        /* Links */
 +
        .igem_2017_content_wrapper a {
 +
            font-weight: bold;
 +
            text-decoration: underline;
 +
            text-decoration-color: #190707;
 +
            color: #3399ff;
 +
            -webkit-transition: all 0.4s ease;
 +
            -moz-transition: all 0.4s ease;
 +
            -ms-transition: all 0.4s ease;
 +
            -o-transition: all 0.4s ease;
 +
            transition: all 0.4s ease;
 +
        }
  
−
    /* Clear the default wiki settings */
+
        /* hover for the links */
 +
        .igem_2017_content_wrapper a:hover {
 +
            text-decoration: none;
 +
            color: #000000;
 +
        }
  
−
    #home_logo, #sideMenu { display:none; }
+
        /* non numbered lists */
−
    #sideMenu, #top_title, .patrollink  {display:none;}
+
        .igem_2017_content_wrapper ul {
−
    #content { width:100%; padding:0px; margin-top:-7px; margin-left:0px;}
+
            padding: 0px 20px;
−
    body {background-color:white; }
+
            font-size: 15px;
−
    #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
+
            font-family: Tahoma, Geneva, sans-serif;
 +
        }
  
 +
        /* numbered lists */
 +
        .igem_2017_content_wrapper ol {
 +
            padding: 0px;
 +
            font-size: 15px;
 +
            font-family: Tahoma, Geneva, sans-serif;
 +
        }
  
 +
        /* Table */
 +
        .igem_2017_content_wrapper table {
 +
            width: 97%;
 +
            margin: 15px 10px;
 +
            border: 2px solid #190707;
 +
            border-collapse: collapse;
 +
        }
  
 +
        /* table cells */
 +
        .igem_2017_content_wrapper td {
 +
            padding: 10px;
 +
            vertical-align: text-top;
 +
            border: 1px solid #190707;
 +
            border-collapse: collapse;
 +
        }
  
−
    /**************************************************************** MENU ***************************************************************/
+
        /* table headers */
−
    /* Wrapper for the menu */
+
        .igem_2017_content_wrapper th {
−
    .igem_2017_menu_wrapper {
+
            padding: 10px;
−
        width: 15%;
+
            vertical-align: text-top;
−
        height:100vh;
+
            border: 1px solid #190707;
−
        position:fixed;
+
            border-collapse: collapse;
−
        right:0;
+
−
        padding:0;
+
−
        float:right;
+
−
        border-left: 1px solid #F8CE63;
+
−
        background-color:#190707;
+
−
        text-align:left;
+
−
        font-family:Tahoma, Geneva, sans-serif;
+
−
        overflow-y: auto;
+
−
        overflow-x: hidden;
+
−
    }
+
  
−
    /* this hides the scrollbar to keep view consistency */
+
         }
−
    .igem_2017_menu_wrappe::-webkit-scrollbar {
+
−
         display: none;
+
−
    }
+
  
 +
        /**********************************LAYOUT CLASSES **********************************/
  
−
    /* styling for links in the menu, removes the line under text */
+
        /* general class for column divs */
−
    .igem_2017_menu_wrapper a {
+
        .igem_2017_content_wrapper .column {
−
        text-decoration: none;
+
            padding: 10px 0px;
−
    }
+
            float: left;
 +
        }
  
 +
        /* class for a full width column */
 +
        .column .full_size {
 +
            width: 100%;
 +
        }
  
−
    /* styling for the images in the menu */
+
        /* styling for images in a full width column*/
−
    .igem_2017_menu_wrapper img {
+
        .column.full_size img {
−
        width: 100%;
+
            width: 97%;
−
    }
+
            padding: 10px 15px;
 +
        }
  
−
    /* styling for the menu buttons */
+
        /* class for a half width column */
−
    .igem_2017_menu_wrapper .menu_button {
+
        .column.half_size {
−
        width: 100%;
+
            width: 50%;
−
         padding: 10px 0px 10px 15px;
+
         }
−
        float:left;
+
−
        border-bottom: 1px solid #F8CE63;
+
−
        font-size: 20px;
+
−
        font-weight: bold;
+
−
        color: #F8CE63;
+
−
        cursor: pointer;
+
−
    }
+
  
 +
        /* styling for images in a half width column*/
 +
        .column.half_size img {
 +
            width: 94.5%;
 +
            padding: 10px 15px;
 +
        }
  
−
    .igem_2017_menu_wrapper .menu_bottom_padding {
+
         /********************************* SUPPORT CLASSES ********************************/
−
         width: 100%;
+
−
        height: 30px;
+
−
        float:left;
+
−
    }
+
  
−
    .menu_button.direct_to_page {
+
        /* class that clears content below*/
−
        padding-left: 36px;
+
        .igem_2017_content_wrapper .clear {
−
    }
+
            clear: both;
 +
        }
  
 +
        /* adds extra spacing when clearing content */
 +
        .igem_2017_content_wrapper .clear.extra_space {
 +
            height: 30px;
 +
        }
  
−
    .igem_2017_menu_wrapper .menu_button .expand_collapse_icon {
+
        /* highlight class, makes content slightly smaller */
−
        width:10%;
+
        .igem_2017_content_wrapper .highlight {
−
        float:left;
+
            margin: 0px 15px;
−
    }
+
            padding: 15px 0px;
 +
        }
  
−
    .igem_2017_menu_wrapper .menu_button .expand_collapse_icon::before {
+
        /* highlight class, adds a gray background */
−
        content: "+";
+
        .igem_2017_content_wrapper .highlight.gray {
−
    }
+
            background-color: #190707;
 +
        }
  
−
    .open::before {
+
        /* highlight with decoration blue line on top */
−
        content: "-" !important;
+
        .igem_2017_content_wrapper .highlight.blue_top {
−
    }
+
            border-top: 4px solid #190707;
 +
        }
  
 +
        /* highlight with a full blue border decoration */
 +
        .igem_2017_content_wrapper .highlight.blue_border {
 +
            border: 4px solid #190707;
 +
        }
  
 +
        /* button class */
 +
        .igem_2017_content_wrapper .button {
 +
            max-width: 35%;
 +
            margin: 30px auto;
 +
            padding: 12px 10px;
 +
            background-color: #190707;
 +
            text-align: center;
 +
            color: #F8CE63;
 +
            -webkit-transition: all 0.4s ease;
 +
            -moz-transition: all 0.4s ease;
 +
            -ms-transition: all 0.4s ease;
 +
            -o-transition: all 0.4s ease;
 +
            transition: all 0.4s ease;
 +
        }
  
−
    /* styling for the menu buttons on hover */
+
        /* styling for button on hover */
−
    .igem_2017_menu_wrapper .menu_button:hover, .igem_2017_menu_wrapper .submenu_button:hover ,  .submenu_button.current_page:hover {
+
        .igem_2017_content_wrapper .button:hover {
−
        background-color: #F8CE63;
+
            background-color: #190707;
−
        text-decoration: none;
+
            color: #F8CE63;
−
        color:#190707;
+
        }
−
    }
+
  
−
    /* styling for the menu button when it is the current page */
+
        /***************************************************** RESPONSIVE STYLING ****************************************************/
−
    .current_page {
+
−
        background-color:#190707  !important;
+
−
        color:#F8CE63 !important;
+
−
    }
+
  
 +
        *, *:before, *:after {
 +
            -webkit-box-sizing: border-box;
 +
            -moz-box-sizing: border-box;
 +
            box-sizing: border-box;
 +
        }
  
−
    /* styling for the submenu buttons */
+
        body {
−
    .igem_2017_menu_wrapper .submenu_button {
+
            overflow-x: hidden;
−
        width: 100%;
+
            margin-top: 80px;
−
        padding: 10px 0px 10px 34px;
+
            letter-spacing: 0.02em;
−
        float:left;
+
         }
−
        background-color:#190707;
+
−
        border-bottom: 1px solid #F8CE63;
+
−
        font-size: 15px;
+
−
         color: #F8CE63;
+
−
        cursor: pointer;
+
−
    }
+
  
−
    /* wrapper for the submenu items, they are hidden by default*/
+
        /* IF THE SCREEN IS LESS THAN 1200PX */
−
    .igem_2017_menu_wrapper .submenu_wrapper {
+
         @media only screen and (max-width: 1024px) {
−
         display:none;
+
−
    }
+
  
−
    /* when the page size is bigger than 800px, this show/hide control is hidden by default */
+
            #content {
−
    .igem_2017_menu_wrapper #display_menu_control {
+
                width: 100%;
−
        display:none;
+
            }
−
        text-align:center;
+
−
    }
+
  
 +
            .igem_2017_content_wrapper {
 +
                width: 100%;
 +
            }
  
−
    /***************************************************** CONTENT OF THE PAGE ****************************************************/
+
            .igem_2017_menu_wrapper {
 +
                width: 100%;
 +
                height: 15%;
 +
                position: relative;
 +
                left: 0;
 +
            }
  
−
    /* Wrapper for the content */
+
            .highlight {
−
    .igem_2017_content_wrapper {
+
                padding: 10px 0px;
−
        width: 81%;
+
            }
−
        margin: 2%;
+
−
        display:block;
+
−
        float:left;
+
−
        background-color: #190707;
+
−
        font-family:Tahoma, Geneva, sans-serif;
+
−
    }
+
  
 +
            .igem_2017_menu_wrapper #display_menu_control {
 +
                display: none;
 +
            }
  
−
    /********************************* HTML STYLING  *********************************/
+
            #menu_content {
 +
                display: block;
 +
            }
  
−
    /* styling for the titles h1 h2 */
+
            .menu_button.direct_to_page {
−
    .igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 {
+
                padding-left: 17px;
−
        color:#F8CE63;
+
            }
−
        padding:15px 0 25px 0;
+
         }
−
         border-bottom: 0;
+
−
    }
+
  
−
    /* styling for the titles  h3 h4 h5 h6*/
+
        /* IF THE SCREEN IS LESS THAN 800PX */
−
    .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 {
+
         @media only screen and (max-width: 800px) {
−
         padding:5px 15px;
+
−
        border-bottom:0;
+
−
    }
+
  
−
    /* font and text */
+
            .igem_2017_content_wrapper {
−
    .igem_2017_content_wrapper p {
+
                width: 100%;
−
        padding: 15px 0;
+
                margin-left: 0;
−
        font-size: 15px;
+
            }
−
    }
+
−
    .igem_2017_content_wrapper img {
+
−
        padding: 15px 0;
+
−
    }
+
  
−
    /* Links */
+
            .column.half_size {
−
    .igem_2017_content_wrapper a {
+
                width: 100%;
−
        font-weight: bold;
+
            }
−
        text-decoration: underline;
+
−
        text-decoration-color: #190707;
+
−
        color:  #3399ff;
+
−
        -webkit-transition: all 0.4s ease;
+
−
        -moz-transition: all 0.4s ease;
+
−
        -ms-transition: all 0.4s ease;
+
−
        -o-transition: all 0.4s ease;
+
−
        transition: all 0.4s ease;
+
−
    }
+
  
−
    /* hover for the links */
+
            .column.full_size img, .column.half_size img {
−
    .igem_2017_content_wrapper a:hover {
+
                width: 100%;
−
        text-decoration:none;
+
                padding: 10px 0;
−
        color:#000000;
+
            }
−
    }
+
  
−
    /* non numbered lists */
+
            .highlight {
−
    .igem_2017_content_wrapper ul {
+
                padding: 15px 5px;
−
        padding:0px 20px;
+
            }
−
        font-size: 15px;
+
−
        font-family:Tahoma, Geneva, sans-serif;
+
−
    }
+
  
−
    /* numbered lists */
+
            .igem_2017_menu_wrapper #display_menu_control {
−
    .igem_2017_content_wrapper ol {
+
                display: block;
−
        padding:0px;
+
            }
−
        font-size: 15px;
+
−
        font-family:Tahoma, Geneva, sans-serif;
+
−
    }
+
  
−
    /* Table */
+
            #menu_content {
−
    .igem_2017_content_wrapper table {
+
                display: none;
−
        width: 97%;
+
            }
−
        margin:15px 10px;
+
−
        border: 2px solid #190707;
+
−
        border-collapse: collapse;
+
−
    }
+
  
−
    /* table cells */
+
            .igem_2017_menu_wrapper .menu_button .expand_collapse_icon {
−
    .igem_2017_content_wrapper  td {
+
                width: 5%;
−
        padding: 10px;
+
            }
−
        vertical-align: text-top;
+
−
        border: 1px solid #190707;
+
−
        border-collapse: collapse;
+
−
    }
+
  
−
    /* table headers */
+
            .menu_bottom_padding {
−
    .igem_2017_content_wrapper th {
+
                display: none;
−
        padding: 10px;
+
            }
−
        vertical-align: text-top;
+
−
        border: 1px solid #190707;
+
−
        border-collapse: collapse;
+
−
        background-color:##F8CE63;
+
−
    }
+
  
 +
            .menu_button.direct_to_page {
 +
                padding-left: 36px;
 +
            }
 +
        }
  
−
    /**********************************LAYOUT CLASSES **********************************/
+
        /* special class that the system uses to make sure the team wants a page to be evaluated */
 +
        .judges-will-not-evaluate {
 +
            width: 96.6%;
 +
            margin: 5px 15px;
 +
            display: block;
 +
            border: 4px solid #3399ff;
 +
            font-weight: bold;
 +
        }
  
−
    /* general class for column divs */
+
        /* CUSTOM BS NAVBAR STYLES */
−
    .igem_2017_content_wrapper .column  {
+
        .navbar {
−
        padding: 10px 0px;
+
            border: 0;
−
         float:left;
+
         }
−
    }
+
  
−
    /* class for a full width column */
+
        .navbar-default {
−
    .column .full_size {
+
            margin-top: 15px;
−
        width:100%;
+
            background-color: #333;
−
    }
+
        }
  
−
    /* styling for images in a full width column*/
+
        .navbar-brand {
−
    .column.full_size img {
+
            padding: 0;
−
        width:97%;
+
            padding-left: 25px;
−
        padding: 10px 15px;
+
            padding-right: 25px;
−
    }
+
        }
  
−
    /* class for a half width column */
+
         .navbar-brand img {
−
    .column.half_size {
+
            height: 49px;
−
         width: 50%;
+
         }
−
    }
+
−
    /* styling for images in a half width column*/
+
−
    .column.half_size img {
+
−
        width: 94.5%;
+
−
         padding: 10px 15px;
+
−
    }
+
  
 +
        .navbar-default .navbar-nav > li > a {
 +
            color: white;
 +
        }
  
 +
        .navbar-default .navbar-nav > li > a:focus,
 +
        .navbar-default .navbar-nav > li > a:hover,
 +
        .navbar-default .navbar-nav > .dropdown > a .caret:hover {
 +
            color: #F8CE63;
 +
        }
  
 +
        .navbar-default .navbar-nav > .open > a,
 +
        .navbar-default .navbar-nav > .open > a:hover,
 +
        .navbar-default .navbar-nav > .open > a:focus {
 +
            color: #F8CE63;
 +
            background-color: #444;
 +
        }
  
−
    /********************************* SUPPORT CLASSES ********************************/
+
        .navbar-default .navbar-nav > .dropdown > a .caret {
 +
            border-top-color: #eee;
 +
            border-bottom-color: #eee;
 +
        }
  
−
    /* class that clears content below*/
+
        .dropdown-menu {
−
    .igem_2017_content_wrapper .clear {
+
            border: 0;
−
         clear:both;
+
         }
−
    }
+
        .dropdown-menu > li > a {
 +
            color: white;
 +
        }
  
 +
        .dropdown-menu > li > a:hover,
 +
        .dropdown-menu > li > a:focus {
 +
            color: #111;
 +
            text-decoration: none;
 +
            background-color: #F8CE63;
 +
        }
  
−
    /* adds extra spacing when clearing content */
+
        .navbar-default .navbar-nav > .dropdown > a:hover .caret,
−
    .igem_2017_content_wrapper  .clear.extra_space {
+
        .navbar-default .navbar-nav > .dropdown > a:focus .caret {
−
        height: 30px;
+
            border-top-color: white;
−
    }
+
            border-bottom-color: white;
 +
        }
  
 +
        @media (max-width: 767px) {
 +
            .navbar-default .navbar-nav .open .dropdown-menu>li>a {
 +
                color: #ddd;
 +
            }
 +
            .navbar-default .navbar-nav .open .dropdown-menu>li>a:focus,
 +
            .navbar-default .navbar-nav .open .dropdown-menu>li>a:hover {
 +
                color: #F8CE63;
 +
            }
 +
        }
  
−
    /* highlight class, makes content slightly smaller */
+
        .navbar-nav>li>.dropdown-menu {
−
    .igem_2017_content_wrapper .highlight {
+
            background-color: #444;
−
        margin: 0px 15px;
+
         }
−
         padding: 15px 0px;
+
−
    }
+
  
 +
        .section {
 +
            margin: 25px 0;
 +
        }
  
−
     /* highlight class, adds a gray background */
+
     </style>
−
     .igem_2017_content_wrapper .highlight.gray {
+
     <script src="https://code.jquery.com/jquery-1.10.2.min.js"></script>
−
        background-color: #190707;
+
     <script src="https://netdna.bootstrapcdn.com/bootstrap/3.0.0/js/bootstrap.min.js"></script>
−
     }
+
  
−
     /* highlight with decoration blue line on top */
+
     <script>
−
    .igem_2017_content_wrapper .highlight.blue_top {
+
        $(document).ready(function(){
−
        border-top: 4px solid #190707;
+
            $("#mw-content-text").removeClass("mw-content-ltr");
−
    }
+
        });
 +
    </script>
  
−
    /* highlight with a full blue border decoration */
 
−
    .igem_2017_content_wrapper .highlight.blue_border {
 
−
        border: 4px solid  #190707;
 
−
    }
 
−
 
−
 
−
    /* button class */
 
−
    .igem_2017_content_wrapper .button{
 
−
        max-width: 35%;
 
−
        margin: 30px auto;
 
−
        padding: 12px 10px;
 
−
        background-color: #190707;
 
−
        text-align: center;
 
−
        color: #F8CE63;
 
−
        -webkit-transition: all 0.4s ease;
 
−
        -moz-transition: all 0.4s ease;
 
−
        -ms-transition: all 0.4s ease;
 
−
        -o-transition: all 0.4s ease; transition: all 0.4s ease;
 
−
    }
 
−
 
−
    /* styling for button on hover */
 
−
    .igem_2017_content_wrapper .button:hover{
 
−
        background-color: #190707;
 
−
        color: #F8CE63;
 
−
    }
 
−
 
−
 
−
 
−
 
−
    /***************************************************** RESPONSIVE STYLING ****************************************************/
 
−
 
−
    /* IF THE SCREEN IS LESS THAN 1200PX */
 
−
    @media only screen and (max-width: 1200px) {
 
−
 
−
        #content {width:100%; }
 
−
        .igem_2017_menu_wrapper {width:15%; right:0;}
 
−
        .highlight {padding:10px 0px;}
 
−
        .igem_2017_menu_wrapper #display_menu_control { display:none; }
 
−
        #menu_content { display:block;}
 
−
        .menu_button.direct_to_page {padding-left: 17px;}
 
−
 
−
    }
 
−
 
−
    /* IF THE SCREEN IS LESS THAN 800PX */
 
−
    @media only screen and (max-width: 800px) {
 
−
 
−
        .igem_2017_menu_wrapper { width:100%; height: 15%; position:relative; left:0%;}
 
−
        .igem_2017_content_wrapper {width:100%; margin-left:0px;}
 
−
        .column.half_size  {width:100%; }
 
−
        .column.full_size img, .column.half_size img {  width: 100%; padding: 10px 0px;}
 
−
        .highlight {padding:15px 5px;}
 
−
        .igem_2017_menu_wrapper #display_menu_control { display:block; }
 
−
        #menu_content { display:none;}
 
−
        .igem_2017_menu_wrapper .menu_button .expand_collapse_icon { width: 5%; }
 
−
        .menu_bottom_padding {display:none;}
 
−
        .menu_button.direct_to_page { padding-left: 36px; }
 
−
    }
 
−
 
−
 
−
 
−
 
−
    /* special class that the system uses to make sure the team wants a page to be evaluated */
 
−
    .judges-will-not-evaluate {
 
−
        width: 96.6%;
 
−
        margin: 5px 15px;
 
−
        display: block;
 
−
        border: 4px solid #3399ff;
 
−
        font-weight: bold;
 
−
    }
 
−
 
−
</style>
 
−
 
−
 
−
<!--- THIS IS WHERE THE HTML BEGINS --->
 
−
 
−
<head>
 
  
 
     <!-- This tells the browser that your page is responsive -->
 
     <!-- This tells the browser that your page is responsive -->
Line 453: Line 515:
  
 
</head>
 
</head>
−
 
+
<body>
  
 
<!--MENU-->
 
<!--MENU-->
−
<div class="igem_2017_menu_wrapper" >
+
<nav class="navbar navbar-default navbar-fixed-top">
−
 
+
     <div class="container-fluid">
−
 
+
         <!-- Brand and toggle get grouped for better mobile display -->
−
 
+
        <div class="navbar-header">
−
     <a href="https://2017.igem.org/Team:Stuttgart">
+
            <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
−
         <img src="https://static.igem.org/mediawiki/2017/4/45/Bildschirmfoto_2017-10-17_um_14.23.20.png">
+
                    data-target="#bs-example-navbar-collapse-1" aria-expanded="false">
−
    </a>
+
                <span class="sr-only">Toggle navigation</span>
−
 
+
                <span class="icon-bar"></span>
−
 
+
                <span class="icon-bar"></span>
−
    <!-- this div is hidden by default and will only be displayed if the screen size is too small -->
+
                <span class="icon-bar"></span>
−
    <div class="menu_button" id="display_menu_control">
+
            </button>
−
        MENU
+
            <a class="navbar-brand" href="https://2017.igem.org/Team:Stuttgart">
−
    </div>
+
                 <img alt="Team:Stuttgart"
−
 
+
                    src="https://static.igem.org/mediawiki/2017/1/18/UNISTUTTGARTLOGO.png">
−
    <div id="menu_content">
+
−
 
+
−
 
+
−
 
+
−
 
+
−
        <a href="https://2017.igem.org/Team:Stuttgart">
+
−
            <div class="menu_button direct_to_page">
+
−
                HOME
+
−
            </div>
+
−
        </a>
+
−
 
+
−
 
+
−
 
+
−
        <div class="menu_button">
+
−
            <div class="expand_collapse_icon"> </div> TEAM
+
−
        </div>
+
−
 
+
−
        <div class="submenu_wrapper" id="team_submenu">
+
−
 
+
−
            <a href="https://2017.igem.org/Team:Stuttgart/Team">
+
−
                 <div class="submenu_button" id="Team_page">
+
−
                    Team
+
−
                </div>
+
 
             </a>
 
             </a>
−
 
−
            <a href="https://2017.igem.org/Team:Stuttgart/Collaborations">
 
−
                <div class="submenu_button"  id="Collaborations_page">
 
−
                    Collaborations
 
−
                </div>
 
−
            </a>
 
−
 
−
 
 
         </div>
 
         </div>
  
 +
        <!-- Collect the nav links, forms, and other content for toggling -->
 +
        <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
 +
            <ul class="nav navbar-nav">
 +
                <li>
 +
                    <a href="https://2017.igem.org/Team:Stuttgart">Home</a>
 +
                </li>
 +
                <li class="dropdown">
 +
                    <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true"
 +
                      aria-expanded="false">Team <span class="caret"></span></a>
 +
                    <ul class="dropdown-menu">
 +
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Team">Team</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Attributions">Attributions</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Sponsoring">Sponoring</a></li>
 +
                        <!--<li role="separator" class="divider"></li>-->
 +
                    </ul>
 +
                </li>
 +
                <li class="dropdown">
 +
                    <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true"
 +
                      aria-expanded="false">Project <span class="caret"></span></a>
 +
                    <ul class="dropdown-menu">
 +
                        <!--<li class="navbar-text navbar-left">Dry Lab</li>-->
 +
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Description">Description</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Background-Information">Background
 +
                            Information</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Protocols">Protocols & Experiments</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Notebook">Notebook</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:Stuttgart/InterLab">Interlab Study</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Model">Modelling</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Results">Results</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:Stuttgart/Parts">Parts</a></li>
 +
                        <!--<li role="separator" class="divider"></li>-->
 +
                    </ul>
 +
                </li>
 +
                <li>
 +
                    <a href="https://2017.igem.org/Team:Stuttgart/Safety">Safety</a>
 +
                </li>
 +
                <li class="dropdown">
 +
                    <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true"
 +
                      aria-expanded="false">Human Practices <span class="caret"></span></a>
 +
                    <ul class="dropdown-menu">
 +
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<h1 align=middle> Notebook </h1>
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<h1 align=middle>Notebook</h1>
+
            <h3>Esterases and Lipases</h3>
−
 
+
            <p>03.08.2017
−
<table>
+
<ul><li>Transformation of BBa_K1149002 and BBa_K1149003</li></ul></p>
−
<td align="left" height=0 bgcolor="#F8CE63"> 
+
<p>04.08.17
−
 
+
<ul><li>Single colonies (BBa_K1149002 and BBa_K1149003) are plated on agar plates and incubated at 37 °C over night</li></ul></p>
−
<p>
+
<p>09.08.17
−
<h3>Esterases and Lipases</h3>
+
<ul><li>Growing of a single colony (BBa_K1149002 and BBa_K1149003) in 5 mL LB media + chloramphenicol (35 µg/mL) at 37 °C</li></ul></p>
−
<br>
+
<p>10.08.17
−
<h4>03.08.2017</h4>
+
−
<ul><li>Transformation of BBa_K1149002 and BBa_K1149003</li></ul>
+
−
<br>
+
−
<h4>04.08.17</h4>
+
−
<ul><li>Single colonies (BBa_K1149002 and BBa_K1149003) are plated on agar plates and incubated at 37 °C over night</li></ul><br>
+
−
<h4>09.08.17</h4>
+
−
<ul><li>Growing of a single colony (BBa_K1149002 and BBa_K1149003) in 5 mL LB media + chloramphenicol (35 µg/mL) at 37 °C</li></ul><br>
+
−
<h4>10.08.17</h4>
+
 
<ul><li>Preparation of miniprep (Jena Biosciences Kit) and glycerol stock (storage: -80 °C) (BBa_K1149002 and BBa_K1149003)</li>
 
<ul><li>Preparation of miniprep (Jena Biosciences Kit) and glycerol stock (storage: -80 °C) (BBa_K1149002 and BBa_K1149003)</li>
 
<li>SOC media preparation</li>
 
<li>SOC media preparation</li>
−
<li>Transformation pet19-LipB</li></ul><br>
+
<li>Transformation pet19-LipB</li></ul></p>
−
<h4>21.08.2017</h4>
+
<p>21.08.2017
−
<ul><li>Preparation: preliminary test of esterase assay with EstCS2: Bba_K1149002 - preparation of o/n cultures (37°C)</li></ul><br>
+
<ul><li>Preparation: preliminary test of esterase assay with EstCS2: Bba_K1149002 - preparation of o/n cultures (37°C)</li></ul></p>
−
<h4>22.08.2017</h4>
+
<p>22.08.2017
−
<ul><li>Preparation: preliminary test of esterase assay with EstCS2: Bba_K1149002 - glycerol stocks and induction with arabinose</li></ul><br>
+
<ul><li>Preparation: preliminary test of esterase assay with EstCS2: Bba_K1149002 - glycerol stocks and induction with arabinose</li></ul></p>
−
<h4>23.08.2017</h4>
+
<p>23.08.2017
−
<ul><li>Preliminary test of esterase assay with EstCS2: Bba_K1149002 (link results) </li></ul><br>
+
<ul><li>Preliminary test of esterase assay with EstCS2: Bba_K1149002 (link results) </li></ul></p>
−
<h4>28.08.2017</h4>
+
<p>28.08.2017
−
<ul><li>Preparation of electro-competent cells </li></ul><br>
+
<ul><li>Preparation of electro-competent cells </li></ul></p>
−
<h4>31.08.2017</h4>
+
<p>31.08.2017
−
<ul><li>Transformation/electroporation with iGEM competent cells test kit DNA (o/n incubation, 37°C - transformation failed)</li></ul><br>
+
<ul><li>Transformation/electroporation with iGEM competent cells test kit DNA (o/n incubation, 37°C - transformation failed)</li></ul></p>
−
<h4>01.09.2017</h4>
+
<p>01.09.2017
−
<ul><li>Transformation/electroporation with pUC19 plasmid (o/n incubation, 37°C - transformation failed)</li></ul><br>
+
<ul><li>Transformation/electroporation with pUC19 plasmid (o/n incubation, 37°C - transformation failed)</li></ul></p>
−
<h4>06.09.2017-08.09.17</h4>
+
<p>06.09.2017-08.09.17
−
<ul><li>Enzyme activity assay (cell pellet and supernatant) with BBa_K1149002 and pet19-LipB</li></ul><br>
+
<ul><li>Enzyme activity assay (cell pellet and supernatant) with BBa_K1149002 and pet19-LipB</li></ul></p>
−
<h4>12.09.2017</h4>
+
<p>12.09.2017
−
<ul><li>Preparation of chemo-competent cells</li></ul><br>
+
<ul><li>Preparation of chemo-competent cells</li></ul></p>
−
<h4>13.09.2017</h4>
+
<p>13.09.2017
−
<ul><li>Transformation with iGEM competent cells test kit DNA and pUC19 plasmid (o/n incubation, 37°C) - transformation successful </li></ul><br>
+
<ul><li>Transformation with iGEM competent cells test kit DNA and pUC19 plasmid (o/n incubation, 37°C) - transformation successful </li></ul></p>
 
<img src="https://static.igem.org/mediawiki/2017/8/87/Stuttgart_compis.jpeg"/img>
 
<img src="https://static.igem.org/mediawiki/2017/8/87/Stuttgart_compis.jpeg"/img>
 
<br>
 
<br>
−
<h4>14.09.2017</h4>
+
<p>14.09.2017
−
<ul><li>Calculation - efficiency of the chemo-competent cells/pUC19:<br>
+
<ul><li>Calculation - efficiency of the chemo-competent cells/pUC19:
−
efficiency E =(56cfu/0.001ng)*1000ng/(µL)=5.6*((10)^7 )cfu/µL</li></ul><br>
+
efficiency E =(56cfu/0.001ng)*1000ng/(µL)=5.6*((10)^7 )cfu/µL</li></ul></p>
−
<h4>18.09.2017</h4>
+
<p>18.09.2017<
−
<ul><li>Preparation InterLab study - transformation of 8 parts into DH5alph E. coli cells</li></ul><br>
+
<ul><li>Preparation InterLab study - transformation of 8 parts into DH5alph E. coli cells</li></ul></p>
−
<h4>19.09.17 – 21.09.17 </h4>
+
<p>19.09.17 – 21.09.17  
 
<ul><li>InterLab study LUDOX measurement</li>
 
<ul><li>InterLab study LUDOX measurement</li>
 
<li>Enzyme activity assay with BBa_K1149002 of the supernatant - measurement in biological triplicates </li>
 
<li>Enzyme activity assay with BBa_K1149002 of the supernatant - measurement in biological triplicates </li>
−
<li>InterLab study Fluorescein measurement</li></ul><br>
+
<li>InterLab study Fluorescein measurement</li></ul></p>
−
<h4>22.09.2017</h4>
+
<p>22.09.2017
−
<ul><li>InterLab study sample measurement (link results)</li></ul><br>
+
<ul><li>InterLab study sample measurement (link results)</li></ul></p>
−
<h4>27.09.17 - 29.09.17</h4>
+
<p>27.09.17 - 29.09.17
−
<ul><li>Enzyme activity assay with pet19-LipB of the supernatant - measurement in biological triplicates</li></ul><br>
+
<ul><li>Enzyme activity assay with pet19-LipB of the supernatant - measurement in biological triplicates</li></ul></p>
−
<h4>06.10.2017</h4>
+
<p>06.10.2017
−
<ul><li>Collaboration iGEM team Heidelberg: preparation mutagenesis plasmid activity assay - preparation of media, antibiotic-stocks and sugar-stocks + agar plates with different antibiotics</li></ul><br>
+
<ul><li>Collaboration iGEM team Heidelberg: preparation mutagenesis plasmid activity assay - preparation of media, antibiotic-stocks and sugar-stocks + agar plates with different antibiotics</li></ul></p>
−
<h4>10.10.2017</h4>
+
<p>10.10.2017
−
<ul><li>Collaboration iGEM team Heidelberg: preparation of different cultures + induction of the cells with arabinose (o/n incubation, 37°C)</li></ul><br>
+
<ul><li>Collaboration iGEM team Heidelberg: preparation of different cultures + induction of the cells with arabinose (o/n incubation, 37°C)</li></ul></p>
−
<h4>11.10.2017</h4>
+
<p>11.10.2017
−
<ul><li>Collaboration iGEM team Heidelberg: spread the o/n cultures on prepared agar plates (o/n incubation, 37°C)</li></ul><br>
+
<ul><li>Collaboration iGEM team Heidelberg: spread the o/n cultures on prepared agar plates (o/n incubation, 37°C)</li></ul></p>
−
<h4>12.10.2017</h4>
+
<p>12.10.2017
−
<ul><li>Gibson Assembly of LipB in psB1C3 backbone</li></ul><br>
+
<ul><li>Gibson Assembly of LipB in psB1C3 backbone</li></ul></p>
−
<h4>19.10.2017</h4>
+
<p>19.10.2017
 
<ul><li>PCR - amplification of LipB - cloning of LipB in the iGEM standard plasmid (BBa_K1149002 is used)</li>
 
<ul><li>PCR - amplification of LipB - cloning of LipB in the iGEM standard plasmid (BBa_K1149002 is used)</li>
−
<li>PCR - amplification of BBa_K1149002 (without EstCS2)</li></ul><br>
+
<li>PCR - amplification of BBa_K1149002 (without EstCS2)</li></ul></p>
−
<h4>20.10.2017</h4>
+
<p>20.10.2017
−
<ul><li>SDS page, Gibson Assembly, Transformation (Zymos) - cloning of LipB in the iGEM standard plasmid (BBa_K1149002 is used)</li></ul><br>
+
<ul><li>SDS page, Gibson Assembly, Transformation (Zymos) - cloning of LipB in the iGEM standard plasmid (BBa_K1149002 is used)</li></ul>
−
</p>
+
</p>  
−
<p>
+
 
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 
<h3>Keratinases</h3>
 
<h3>Keratinases</h3>
 
<br>
 
<br>
Line 903: Line 777:
 
</p>
 
</p>
  
−
</div>
+
 
 +
 
 +
</body>
 
</html>
 
</html>

Revision as of 11:48, 29 October 2017

Notebook

Esterases and Lipases

03.08.2017

  • Transformation of BBa_K1149002 and BBa_K1149003

04.08.17

  • Single colonies (BBa_K1149002 and BBa_K1149003) are plated on agar plates and incubated at 37 °C over night

09.08.17

  • Growing of a single colony (BBa_K1149002 and BBa_K1149003) in 5 mL LB media + chloramphenicol (35 µg/mL) at 37 °C

10.08.17

  • Preparation of miniprep (Jena Biosciences Kit) and glycerol stock (storage: -80 °C) (BBa_K1149002 and BBa_K1149003)
  • SOC media preparation
  • Transformation pet19-LipB

21.08.2017

  • Preparation: preliminary test of esterase assay with EstCS2: Bba_K1149002 - preparation of o/n cultures (37°C)

22.08.2017

  • Preparation: preliminary test of esterase assay with EstCS2: Bba_K1149002 - glycerol stocks and induction with arabinose

23.08.2017

  • Preliminary test of esterase assay with EstCS2: Bba_K1149002 (link results)

28.08.2017

  • Preparation of electro-competent cells

31.08.2017

  • Transformation/electroporation with iGEM competent cells test kit DNA (o/n incubation, 37°C - transformation failed)

01.09.2017

  • Transformation/electroporation with pUC19 plasmid (o/n incubation, 37°C - transformation failed)

06.09.2017-08.09.17

  • Enzyme activity assay (cell pellet and supernatant) with BBa_K1149002 and pet19-LipB

12.09.2017

  • Preparation of chemo-competent cells

13.09.2017

  • Transformation with iGEM competent cells test kit DNA and pUC19 plasmid (o/n incubation, 37°C) - transformation successful


14.09.2017

  • Calculation - efficiency of the chemo-competent cells/pUC19: efficiency E =(56cfu/0.001ng)*1000ng/(µL)=5.6*((10)^7 )cfu/µL

18.09.2017<

  • Preparation InterLab study - transformation of 8 parts into DH5alph E. coli cells

19.09.17 – 21.09.17

  • InterLab study LUDOX measurement
  • Enzyme activity assay with BBa_K1149002 of the supernatant - measurement in biological triplicates
  • InterLab study Fluorescein measurement

22.09.2017

  • InterLab study sample measurement (link results)

27.09.17 - 29.09.17

  • Enzyme activity assay with pet19-LipB of the supernatant - measurement in biological triplicates

06.10.2017

  • Collaboration iGEM team Heidelberg: preparation mutagenesis plasmid activity assay - preparation of media, antibiotic-stocks and sugar-stocks + agar plates with different antibiotics

10.10.2017

  • Collaboration iGEM team Heidelberg: preparation of different cultures + induction of the cells with arabinose (o/n incubation, 37°C)

11.10.2017

  • Collaboration iGEM team Heidelberg: spread the o/n cultures on prepared agar plates (o/n incubation, 37°C)

12.10.2017

  • Gibson Assembly of LipB in psB1C3 backbone

19.10.2017

  • PCR - amplification of LipB - cloning of LipB in the iGEM standard plasmid (BBa_K1149002 is used)
  • PCR - amplification of BBa_K1149002 (without EstCS2)

20.10.2017

  • SDS page, Gibson Assembly, Transformation (Zymos) - cloning of LipB in the iGEM standard plasmid (BBa_K1149002 is used)

Keratinases


26.07.2017

  • Transformation of kerUS (BBa_K1498000)
  • Preparation of antibiotic stock solutions: chloramphenicol (35 mg/mL) and ampicillin (100 mg/mL)

27.07.2017

  • Growing of a single colony (kerUS (BBa_K1498000)) in 5 mL LB media chloramphenicol (35 µg/mL) at 37 °C
  • Single colonies are plated on agar plates and incubated at 37 °C over night

28.07.2017

  • Preparation of: miniprep (Jena Biosciences Kit)and glycerol stock (kerUS (BBa_K1498000))

30.08.2017

  • Transformation of psB1K3-KerP

30.08.2017

  • Transformation of KerUS (BBa_K1498000)

12.10.2017

  • Gibson Assembly KerA-ompA, ompA-KerA, KerUS-ompA and ompA-KerUS in psB1C3 backbone

16.10.2017

  • Preparation semi-quantitative keratinase-assay: spread kerUS, KerA and KerP on chloramphenicol/kanamycin agar plates (o/n incubation, 37°C)
  • Preparation skim-milk-plate assay: preparation of skim-milk-agar-plates with chloramphenicol/kanamycin

    • 18.10.2017

    • Preparation semi-quantitative keratinase-assay: preparation of o/n cultures (37°C)
  • Preparation skim-milk-plate assay: preparation of o/n cultures (37°C)

    • 19.10.2017

    • Semi-quantitative keratinase-assay: add 0.005 g of dried hair (65 °C, 1h) to o/n cultures (KerUS and KerA are induced with 1mM IPTG before) - 5 days incubation, 37°C (link results)
    • Skim-milk-plate assay kerP - spread on prepared skim-milk agar plates (supernatant and cells) - 4 days incubation, room temperature (link results)

    20.10.2017

    • Skim-milk-plate assay kerUS and KerA - spread on prepared skim-milk agar plates (supernatant and cells) - 4 days incubation, room temperature (link results)
    • Sequencing of keratinase plasmids: pSB1C3-KerA-ompA, pSB1C3-KerUS-ompA, pSB1C3-KerA_Kanada and pSB1C3-KerUS-Kanada< /li>

    Rose and Limonene Fragrance


    28.08.2017

    • Preparation of LB-Agar plates with kanamycin (50 µg/mL)
    • Sequencing of rose fragrance plasmids from Guo et al. (pET28a-KDC-YjgB-ARO8 and pET28a-ATF1)

    13.09.2017

    • Overlap-PCR of 1.pBAD (BBa_K206000), 2.KDC-YjgB-ARO8, 3.ATF1 and 4.pBAD-KDC-YjgB-ARO8
    • PCR-Purification and agarose-gel-electrophoresis of PCR products

    • PCR 4 (pBAD-KDC-YjgB-ARO8) not successful

    19.09.2017

    • Preparation of LB-Agar plates with kanamycin (50 µg/mL) and chloramphenicol (35 µg/mL)
    • Repeat of PCR 4 (pBAD-KDC-YjgB-ARO8)
    • PCR-Purification and agarose-gel-electrophoresis of PCR product 4

    • PCR 4 (pBAD-KDC-YjgB-ARO8) not successful
    • Transformation of Limonene-plasmid and over-night incubation on agar-plates with kanamycin (50 µg/mL) at 37°C

    21.09.2017

    • Gibson Assembly of rose PCR-overlap products pBAD, KDC-YjgB-ARO8 and ATF1 in psB1K3 backbone
    • Transformation of assembled rose-plasmid and over-night incubation on agar-plates with kanamycin (50 µg/mL) at 37°C

    22.09.2017

    • Rose-plasmid Transformation successful
    • Single colonies are plated on agar plates and incubated at 37 °C over night

    25.09.2017

    • Verification of transformed rose-plasmid by colony-PCR
    • Agarose-gel-electrophoresis of colony-PCR product - colony-PCR not successful

    26.09.2017

    • Repeat of colony-PCR with transformed rose-plasmid
    • Agarose-gel-electrophoresis of colony-PCR product - colony-PCR not successful

    27.09.2017

    • Repeat of colony-PCR with transformed rose-plasmid
    • Agarose-gel-electrophoresis of colony-PCR product - colony-PCR not successful


    28.09.2017

    • Mini-prep of transformed rose-plasmid
    • Restriction assay of isolated rose-plasmid, cut with SpeI
    • Verification of restriction product by agarose-gel-electrophoresis - not successful

    29.09.2017

    • Repeat Gibson Assembly of rose PCR-overlap products pBAD, KDC-YjgB-ARO8 and ATF1
    • Transformation of assembled rose-plasmid and over-night incubation on agar-plates with kanamycin (50 µg/mL) at 37°C - transformation not successful

    05.10.2017

    • Repeat Restriction assay of isolated rose-plasmid, cut with EcoRI - not successful
    • Verification of restriction product by agarose-gel-electrophoresis - not successful

    12.10.2017

    • Gibson Assembly of limonene PCR-products () in psB1C3 backbone
    • Transformation of assembled rose-plasmid and over-night incubation on agar-plates with kanamycin (50 µg/mL) at 37°C - transformation not successful

    18.10.2017

    • Repeat of colony-PCR with transformed rose-plasmid, temperature range from 58-70 °C
    • Agarose-gel-electrophoresis of colony-PCR product - colony-PCR not successful
    • M9 media preparation

    20.10.2017

    • Repeat overlap-PCR of pBad and KDC-YjgB-ARO8
    • Verification of PCR products by agarose-gel-electrophoresis

    • PCR (pBAD-KDC-YjgB-ARO8) not successful

    23.10.2017

    • Sequencing of transformed rose fragrance plasmid (pSB1K3-pBad-KDC-YjgB-ARO8-ATF1)
    • Sequencing of transformed limonene fragrance plasmid (pSB1C3-pBad)
    • Growing of single rose-colonies (pSB1K3-pBad-KDC-YjgB-ARO8-ATF1) in 5 mL LB media with kanamycin (50 µg/mL) at 37 °C over night

    24.10.2017

    • Collecting rose-plasmid-cells by centrifugation and growing in M9-media to OD(600)=0,8
    • Preparation of different cultures (different Phenylalanine-concentrations + induction of the cells with arabinose (o/n incubation, 37°C)