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XiaojiangLi (Talk | contribs) |
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<a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Project <span class="caret"></span></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Project <span class="caret"></span></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
− | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/ | + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Design">Design</a></li> |
− | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/ | + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Applied_Design">Background</a></li> |
− | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/ | + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/InterLab">Interlab</a></li> |
− | <li class="chooseone | + | <li class="chooseone"><a href="https://2017.igem.org/Team:FAFU-CHINA/Plant">Module 1: Phyto-route</a></li> |
− | <li class="choosetwo | + | <li class="choosetwo active"><a href="#sss2">Module 2: Metal-trap</a></li> |
− | <li class="choosethree | + | <li class="choosethree"><a href="#sss3">Module 3: Safeguard</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Achievements <span class="caret"></span></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Achievements <span class="caret"></span></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
− | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/ | + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Demonstrate">Demonstrate</a></li> |
− | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/ | + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Judging_Form">Judging Form</a></li> |
− | < | + | <!--<li><a href="https://2017.igem.org/Team:Example2/Entrepreneurship">Community</a></li>--> |
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</ul> | </ul> | ||
</li> | </li> | ||
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<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Collaborations">Collaborations</a></li> | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Collaborations">Collaborations</a></li> | ||
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Protocols ">Protocols</a></li> | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Protocols ">Protocols</a></li> | ||
− | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Notebook"> | + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Notebook">Notebook</a></li> |
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</ul> | </ul> | ||
</li> | </li> | ||
+ | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Parts">Parts</a></li> | ||
<!-- <li class="dropdown"> | <!-- <li class="dropdown"> | ||
<a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Results <span class="caret"></span></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Results <span class="caret"></span></a> | ||
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<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Model">Model</a></li> | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Model">Model</a></li> | ||
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Human_Practices">Human Practice</a></li> | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Human_Practices">Human Practice</a></li> | ||
− | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Team">Team</a></li> | + | <li class="dropdown"> |
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Team <span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Team">Team members</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Attributions">Attributions</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
</ul> | </ul> | ||
</div><!-- /.navbar-collapse --> | </div><!-- /.navbar-collapse --> | ||
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<style> | <style> | ||
#bodydii{ | #bodydii{ | ||
− | margin: | + | margin:0px auto; |
padding:50px; | padding:50px; | ||
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background-color:#cee1aa; | background-color:#cee1aa; | ||
} | } | ||
− | + | p{ | |
− | + | font-size: 20px; | |
+ | font-family: -webkit-pictograph; | ||
} | } | ||
− | + | h2{ | |
+ | color: #6a8d2c ; | ||
+ | margin-top:100px; | ||
+ | margin-left: 150px; | ||
+ | margin-right: 150px ; | ||
+ | font-family: monospace; | ||
+ | font-weight: bold; | ||
+ | font-size: 60px; | ||
+ | text-align: center; | ||
color:#6a8d2c; | color:#6a8d2c; | ||
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</style> | </style> | ||
<script> | <script> | ||
$(function(){ | $(function(){ | ||
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function fuunn2(){ | function fuunn2(){ | ||
− | $(' | + | $('#chooset2,#chooset3,#h12,#h13').addClass('display'); |
$('#chooset2,#h12').removeClass('display'); | $('#chooset2,#h12').removeClass('display'); | ||
$('.active').removeClass('active'); | $('.active').removeClass('active'); | ||
− | $('. | + | $('.choosetwo,.dropdown11').addClass('active'); |
} | } | ||
function fuunn3(){ | function fuunn3(){ | ||
− | $(' | + | $('#chooset2,#chooset3,#h12,#h13').addClass('display'); |
$('#chooset3,#h13').removeClass('display'); | $('#chooset3,#h13').removeClass('display'); | ||
$('.active').removeClass('active'); | $('.active').removeClass('active'); | ||
− | $('. | + | $('.choosethree,.dropdown11').addClass('active'); |
} | } | ||
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$('.choosetwo').bind('click',fuunn2); | $('.choosetwo').bind('click',fuunn2); | ||
$('.choosethree').bind('click',fuunn3); | $('.choosethree').bind('click',fuunn3); | ||
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var $urrl=window.location.href; | var $urrl=window.location.href; | ||
− | if($urrl=='https://2017.igem.org/Team:FAFU-CHINA/Experiments# | + | if($urrl=='https://2017.igem.org/Team:FAFU-CHINA/Experiments#sss2'){ |
$('.active').removeClass('active'); | $('.active').removeClass('active'); | ||
− | $('. | + | $('.choosetwo,.dropdown11').addClass('active'); |
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}else if($urrl=='https://2017.igem.org/Team:FAFU-CHINA/Experiments#sss3'){ | }else if($urrl=='https://2017.igem.org/Team:FAFU-CHINA/Experiments#sss3'){ | ||
$('.active').removeClass('active'); | $('.active').removeClass('active'); | ||
− | $('.choosethree').addClass('active'); | + | $('.choosethree,.dropdown11').addClass('active'); |
} | } | ||
}); | }); | ||
</script> | </script> | ||
− | <div style="background-color: #F1E6CE; width:80%; margin: | + | <div style="background-color: #F1E6CE; width:80%; margin:0px auto;"> |
− | <div | + | <br/><br/><br/><br/><br/><br/><br/><br/><br/><br/> |
− | < | + | <div> |
− | < | + | <h2 id="h12">Module 2: Metal-trap</h2> |
− | < | + | <h2 id="h13" class="display">Module 3: Safeguard</h2><br/><br/> |
+ | <hr style="border:1px solid #D4CCCC; clear:both;width: 80%; margin: 0 auto"/> | ||
</div> | </div> | ||
<div id="bodydii"> | <div id="bodydii"> | ||
<ul class="nav nav-pills" style="width:60%; margin:0 auto;"> | <ul class="nav nav-pills" style="width:60%; margin:0 auto;"> | ||
− | <li role="presentation" class=" | + | <li role="presentation" class="chooseone"><a href="https://2017.igem.org/Team:FAFU-CHINA/Plant">Module 1: Phyto-route</a></li> |
− | <li role="presentation" class="choosetwo"><a href="#sss2">Module 2: Metal-trap</a></li> | + | <li role="presentation" class="active choosetwo"><a href="#sss2">Module 2: Metal-trap</a></li> |
<li role="presentation" class="choosethree"><a href="#sss3">Module 3: Safeguard</a></li> | <li role="presentation" class="choosethree"><a href="#sss3">Module 3: Safeguard</a></li> | ||
</ul> | </ul> | ||
− | <div id=" | + | |
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <div id="chooset2" style="font-size:18px;"></div> |
− | < | + | <div id="chooset3" style="font-size:18px;" class="display"> |
− | + | ||
+ | |||
+ | |||
+ | <p style="text-align: center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/b/bb/T--FAFU-CHINA--010.png" style="width:50%;border-radius:20px 20px 20px 20px"/> | ||
+ | </p> | ||
+ | <p style="font-size:15px; text-align: center">Fig 1 Toggle switch, a mechanism that is triggered if a human operator becomes incapacitated, allows cells to live as long as an operator compound (anhydrotetracycline, ATc) is present.</p><br/><br/> | ||
+ | |||
+ | |||
+ | <hr style="border:1px solid #D4CCCC;"/> | ||
+ | <p><strong> Biochtainment systems</strong> that couple enviromental sensing with circuit-based control of cell viability could be used to prevent escape of genetically modified microbes into the enviroment. In order to guarantee our chassis’ safety of the application in reality , we have taken a robust and significant strategy, dubbed ‘Toggle Switch’(BB0J_00part页面), based on a circuit which the LacI and TetR transcription factors are reciprocally repressive.</p> | ||
<hr style="border:1px solid #D4CCCC; clear:both;"/> | <hr style="border:1px solid #D4CCCC; clear:both;"/> | ||
<h2 style="color: #6a8d2c">Overview</h2> | <h2 style="color: #6a8d2c">Overview</h2> | ||
− | <p> | + | <p> For Human Practice-Discussion Panel(链接到HP的部分), we have come up with a common question to President Shi that “If our project needs to be applied in practice, what will be the specific operation processes? And of what aspects shall we be conscious? ”. It is a essential reminder of his answers tha there is the need for constant care. |
− | + | We decided to customize a ‘kill switch’ as a safeguard for our project, simultaneously harbors a neutral statet that allows billions of microbes to happily thrive and avoid unexpectedly escaping of GEMs into the enviroment and transferring of non-natural plasmid DNA to existing soil bacteria. | |
− | + | ‘Toggle Switch’, based on a circuit which the LacI and TetR transcription factors are reciprocally repressive, but in which the expression off TetR is favoured owing to modifications in the strength of the ribosommal binding sites of the two transcription factors. Inhibition of TetR expression by anhydrotetracycline(ATc), a compound that is not normally found in nature, is necessary of expression of LacI. LacI directly inhibits expression of a lethal toxin or indirectly prevents inhibition of the expression of our target genes. Removal of ATc from the enviroment activates the expression of TetR, which leads to cell death. | |
− | + | For more information about lab, please click here.(链接到lab页面) | |
− | + | </p> | |
− | + | <hr style="border:1px solid #D4CCCC; clear:both;"/> | |
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− | <hr style="border:1px solid #D4CCCC;"/> | + | |
<h2 style="color: #6a8d2c">Reference</h2> | <h2 style="color: #6a8d2c">Reference</h2> | ||
− | + | <p>Haynes, K. A. (2016). Synthetic biology: Building genetic containment. Nature Publishing Group, 12(2), 55–56. https://doi.org/10.1038/nchembio.2004</p> | |
− | + | <p>Osório, J. (2015). Genetic kill switches — a matter of life or death. Nature Publishing Group, 1979(December), 2015. https://doi.org/10.1038/nrg.2015.29</p> | |
− | + | <p>Chan, C. T. Y., Lee, J. W., Cameron, D. E., Bashor, C. J., & Collins, J. J. (2015). for bacterial containment. Nature Chemical Biology, (December), 1–7. https://doi.org/10.1038/nchembio.1979 | |
− | + | </p> | |
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− | <p>Haynes, K. A. (2016). Synthetic biology: Building genetic containment. Nature Publishing Group, 12(2), 55–56. https://doi.org/10.1038/nchembio.2004</p> | + | |
− | <p>Osório, J. (2015). Genetic kill switches — a matter of life or death. Nature Publishing Group, 1979(December), 2015. https://doi.org/10.1038/nrg.2015.29</p> | + | |
− | <p>Chan, C. T. Y., Lee, J. W., Cameron, D. E., Bashor, C. J., & Collins, J. J. (2015). for bacterial containment. Nature Chemical Biology, (December), 1–7. https://doi.org/10.1038/nchembio.1979 | + | |
− | </p> | + | |
+ | |||
</div> | </div> | ||
</div> | </div> |
Revision as of 01:51, 29 October 2017
Module 2: Metal-trap
Module 3: Safeguard
Fig 1 Toggle switch, a mechanism that is triggered if a human operator becomes incapacitated, allows cells to live as long as an operator compound (anhydrotetracycline, ATc) is present.
Biochtainment systems that couple enviromental sensing with circuit-based control of cell viability could be used to prevent escape of genetically modified microbes into the enviroment. In order to guarantee our chassis’ safety of the application in reality , we have taken a robust and significant strategy, dubbed ‘Toggle Switch’(BB0J_00part页面), based on a circuit which the LacI and TetR transcription factors are reciprocally repressive.
Overview
For Human Practice-Discussion Panel(链接到HP的部分), we have come up with a common question to President Shi that “If our project needs to be applied in practice, what will be the specific operation processes? And of what aspects shall we be conscious? ”. It is a essential reminder of his answers tha there is the need for constant care. We decided to customize a ‘kill switch’ as a safeguard for our project, simultaneously harbors a neutral statet that allows billions of microbes to happily thrive and avoid unexpectedly escaping of GEMs into the enviroment and transferring of non-natural plasmid DNA to existing soil bacteria. ‘Toggle Switch’, based on a circuit which the LacI and TetR transcription factors are reciprocally repressive, but in which the expression off TetR is favoured owing to modifications in the strength of the ribosommal binding sites of the two transcription factors. Inhibition of TetR expression by anhydrotetracycline(ATc), a compound that is not normally found in nature, is necessary of expression of LacI. LacI directly inhibits expression of a lethal toxin or indirectly prevents inhibition of the expression of our target genes. Removal of ATc from the enviroment activates the expression of TetR, which leads to cell death. For more information about lab, please click here.(链接到lab页面)
Reference
Haynes, K. A. (2016). Synthetic biology: Building genetic containment. Nature Publishing Group, 12(2), 55–56. https://doi.org/10.1038/nchembio.2004
Osório, J. (2015). Genetic kill switches — a matter of life or death. Nature Publishing Group, 1979(December), 2015. https://doi.org/10.1038/nrg.2015.29
Chan, C. T. Y., Lee, J. W., Cameron, D. E., Bashor, C. J., & Collins, J. J. (2015). for bacterial containment. Nature Chemical Biology, (December), 1–7. https://doi.org/10.1038/nchembio.1979