Difference between revisions of "Team:NYMU-Taipei/Measurement"
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<h1>Measurement</h1> | <h1>Measurement</h1> | ||
| − | <p> In our project, we used a photo-bioreactor for microbial cell growth measurement. The photo-bioreactor is originally used to determine gene expression during cell growth, but we used it to measure the growth curve during continuous cultivation in BG-11 of two microalgae in our project. | + | <p> In our project, we used a <b>photo-bioreactor</b> for microbial cell growth measurement. The photo-bioreactor is originally used to determine gene expression during cell growth, but we used it to measure the growth curve during continuous cultivation in BG-11 of two microalgae in our project. |
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<li>Once calibrated, the photo-bioreactor can automatically cultivate microalgae, measuring concentration in a long period of time, and restores data back to computer automatically. It can eliminate the long-term manual measurements and saves more time for researchers. | <li>Once calibrated, the photo-bioreactor can automatically cultivate microalgae, measuring concentration in a long period of time, and restores data back to computer automatically. It can eliminate the long-term manual measurements and saves more time for researchers. | ||
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| + | (See more detail: <a href="https://2017.igem.org/Team:NYMU-Taipei/Nitrogen_starvation">Nitrogen Starvation - Experiments - Automatic Measurement</a>) | ||
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Revision as of 09:32, 29 October 2017
Measurement
In our project, we used a photo-bioreactor for microbial cell growth measurement. The photo-bioreactor is originally used to determine gene expression during cell growth, but we used it to measure the growth curve during continuous cultivation in BG-11 of two microalgae in our project.
The followings are the advantages of using this measurement method:
- Due to the opened command lines and cheapness of building Arduino devices, the photo-bioreactor we used is cheaper than general photoreactors or spectrophotometers for researchers to measure absorbance.
- Compared to recording a single record every time by spectrophotometer, the photo-bioreactor can eliminate batch-to-batch variation after measuring several time points continuously, which significantly improves the accuracy of measurement.
- Once calibrated, the photo-bioreactor can automatically cultivate microalgae, measuring concentration in a long period of time, and restores data back to computer automatically. It can eliminate the long-term manual measurements and saves more time for researchers.
