Line 39: | Line 39: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368003">BBa_2368003</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368003">BBa_2368003</a> | ||
</td> | </td> | ||
− | <td | + | <td>T1R2</td> |
− | <td>Human | + | <td>Human Sweet-receptor</td> |
<td>2520</td> | <td>2520</td> | ||
</tr> | </tr> | ||
Line 47: | Line 47: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368004">BBa_2368004</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368004">BBa_2368004</a> | ||
</td> | </td> | ||
− | <td | + | <td>T1R3</td> |
− | <td>Human | + | <td>Human Sweet-receptor</td> |
<td>1989</td> | <td>1989</td> | ||
</tr> | </tr> | ||
Line 55: | Line 55: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368007">BBa_2368007</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368007">BBa_2368007</a> | ||
</td> | </td> | ||
− | <td>< | + | <td>P<sub>Gal 1/10<sub>-T1R2-CYC1t</td> |
− | <td>Completed expression of the human | + | <td>Completed expression of the human Sweet-receptor</td> |
<td>3449</td> | <td>3449</td> | ||
</tr> | </tr> | ||
Line 63: | Line 63: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368008">BBa_2368008</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368008">BBa_2368008</a> | ||
</td> | </td> | ||
− | <td>< | + | <td>P<sub>Gal 1/10<sub>-T1R3-ADH1</td> |
− | <td>Completed expression of the human | + | <td>Completed expression of the human Sweet-receptor</td> |
<td>2814</td> | <td>2814</td> | ||
</tr> | </tr> | ||
Line 71: | Line 71: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368023">BBa_2368023</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368023">BBa_2368023</a> | ||
</td> | </td> | ||
− | <td | + | <td>MYC+T1R2 overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>349</td> | <td>349</td> | ||
Line 79: | Line 79: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368009">BBa_2368009</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368009">BBa_2368009</a> | ||
</td> | </td> | ||
− | <td | + | <td>HIS+T1R2 overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>50</td> | <td>50</td> | ||
Line 87: | Line 87: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368024">BBa_2368024</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368024">BBa_2368024</a> | ||
</td> | </td> | ||
− | <td | + | <td>HIS+T1R3 overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>187</td> | <td>187</td> | ||
Line 95: | Line 95: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368011">BBa_2368011</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368011">BBa_2368011</a> | ||
</td> | </td> | ||
− | <td | + | <td>FLAG+T1R3 overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>50</td> | <td>50</td> | ||
Line 103: | Line 103: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368012">BBa_2368012</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368012">BBa_2368012</a> | ||
</td> | </td> | ||
− | <td | + | <td>YCP+T1R2 overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>740</td> | <td>740</td> | ||
Line 112: | Line 112: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368013">BBa_2368013</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368013">BBa_2368013</a> | ||
</td> | </td> | ||
− | <td | + | <td>YCP+T1R3 overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>740</td> | <td>740</td> | ||
Line 120: | Line 120: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368014">BBa_2368014</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368014">BBa_2368014</a> | ||
</td> | </td> | ||
− | <td | + | <td>BCP+T1R2 overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>710</td> | <td>710</td> | ||
Line 128: | Line 128: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368015">BBa_2368015</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368015">BBa_2368015</a> | ||
</td> | </td> | ||
− | <td | + | <td>GFP+T1R2 overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>699</td> | <td>699</td> | ||
Line 136: | Line 136: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368020">BBa_2368020</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368020">BBa_2368020</a> | ||
</td> | </td> | ||
− | <td | + | <td>BCP+T1R3 overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>710</td> | <td>710</td> | ||
Line 154: | Line 154: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368017">BBa_2368017</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368017">BBa_2368017</a> | ||
</td> | </td> | ||
− | <td>500bp Homologous | + | <td>500bp Homologous arm+His</td> |
− | <td> | + | <td>knock out the <i>sst2</i> gene</td> |
<td>2050</td> | <td>2050</td> | ||
</tr> | </tr> | ||
Line 162: | Line 162: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368018">BBa_2368018</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368018">BBa_2368018</a> | ||
</td> | </td> | ||
− | <td>500bp Homologous | + | <td>500bp Homologous arm+Ura</td> |
− | <td> | + | <td>knock out the <i>far1</i> gene</td> |
<td>2050</td> | <td>2050</td> | ||
</tr> | </tr> | ||
Line 170: | Line 170: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368016">BBa_2368016</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368016">BBa_2368016</a> | ||
</td> | </td> | ||
− | <td>500bp Homologous | + | <td>500bp Homologous arm+Trp</td> |
− | <td> | + | <td>knock out the <i>ste2</i> gene</td> |
<td>2071</td> | <td>2071</td> | ||
</tr> | </tr> | ||
Line 201: | Line 201: | ||
<tr> | <tr> | ||
− | <td colspan="4" style="line-height: 2rem; text-transform: uppercase; font-weight: bold;"> | + | <td colspan="4" style="line-height: 2rem; text-transform: uppercase; font-weight: bold;">Detection circuit</td> |
</tr> | </tr> | ||
Line 219: | Line 219: | ||
</td> | </td> | ||
<td><i>P<sub>fus</sub></i>(4 STE12 binding sites)</td> | <td><i>P<sub>fus</sub></i>(4 STE12 binding sites)</td> | ||
− | <td> | + | <td>Engineered promoter</td> |
<td>223</td> | <td>223</td> | ||
</tr> | </tr> | ||
Line 227: | Line 227: | ||
</td> | </td> | ||
<td><i>P<sub>fus</sub></i>(6 STE12 binding sites)</td> | <td><i>P<sub>fus</sub></i>(6 STE12 binding sites)</td> | ||
− | <td> | + | <td>Engineered promoter</td> |
<td>249</td> | <td>249</td> | ||
</tr> | </tr> | ||
Line 234: | Line 234: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368006">BBa_2368006</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368006">BBa_2368006</a> | ||
</td> | </td> | ||
− | <td><i>P<sub>fus</sub></i>+< | + | <td><i>P<sub>fus</sub></i>+mRFP+<>CYC1t</i></td> |
− | <td>The | + | <td>The reporter</td> |
<td>1375</td> | <td>1375</td> | ||
</tr> | </tr> | ||
Line 243: | Line 243: | ||
</td> | </td> | ||
<td><i>CYC1t</i> </td> | <td><i>CYC1t</i> </td> | ||
− | <td>A terminator | + | <td>A terminator in the yeast</td> |
<td>231</td> | <td>231</td> | ||
</tr> | </tr> | ||
Line 251: | Line 251: | ||
</td> | </td> | ||
<td> <i>P<sub>fus</sub></i>(2 STE12 binding sites)</td> | <td> <i>P<sub>fus</sub></i>(2 STE12 binding sites)</td> | ||
− | <td> | + | <td>Engineered promoter</td> |
<td>213</td> | <td>213</td> | ||
</tr> | </tr> |
Revision as of 07:41, 28 October 2017
Biobrick | Name | Description | Length |
---|---|---|---|
Receptor expression | |||
BBa_2368003 | T1R2 | Human Sweet-receptor | 2520 |
BBa_2368004 | T1R3 | Human Sweet-receptor | 1989 |
BBa_2368007 | PGal 1/10-T1R2-CYC1t | Completed expression of the human Sweet-receptor | 3449 |
BBa_2368008 | PGal 1/10-T1R3-ADH1 | Completed expression of the human Sweet-receptor | 2814 |
BBa_2368023 | MYC+T1R2 overlap | Marker genes of the Sweet-receptor | 349 |
BBa_2368009 | HIS+T1R2 overlap | Marker genes of the Sweet-receptor | 50 |
BBa_2368024 | HIS+T1R3 overlap | Marker genes of the Sweet-receptor | 187 |
BBa_2368011 | FLAG+T1R3 overlap | Marker genes of the Sweet-receptor | 50 |
BBa_2368012 | YCP+T1R2 overlap | Marker genes of the Sweet-receptor | 740 |
BBa_2368013 | YCP+T1R3 overlap | Marker genes of the Sweet-receptor | 740 |
BBa_2368014 | BCP+T1R2 overlap | Marker genes of the Sweet-receptor | 710 |
BBa_2368015 | GFP+T1R2 overlap | Marker genes of the Sweet-receptor | 699 |
BBa_2368020 | BCP+T1R3 overlap | Marker genes of the Sweet-receptor | 710 |
Host engineering | |||
BBa_2368017 | 500bp Homologous arm+His | knock out the sst2 gene | 2050 |
BBa_2368018 | 500bp Homologous arm+Ura | knock out the far1 gene | 2050 |
BBa_2368016 | 500bp Homologous arm+Trp | knock out the ste2 gene | 2071 |
BBa_2368021 | Modified Gα Schizosaccharomyces pombe | Signal transduction | 1421 |
BBa_2368022 | Modified Gα Caenorhabditis elegans | Signal transduction | 1418 |
BBa_2368028 | Gα | Signal transduction | 1421 |
Detection circuit | |||
BBa_2368005 | Pfus | Induced promoter | 438 |
BBa_2368026 | Pfus(4 STE12 binding sites) | Engineered promoter | 223 |
BBa_2368027 | Pfus(6 STE12 binding sites) | Engineered promoter | 249 |
BBa_2368006 | Pfus+mRFP+<>CYC1t | The reporter | 1375 |
BBa_2368002 | CYC1t | A terminator in the yeast | 231 |
BBa_2368029 | Pfus(2 STE12 binding sites) | Engineered promoter | 213 |