Difference between revisions of "Team:Sydney Australia/Parts"
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<link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet"> | <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet"> | ||
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| − | <div class="row" style="height:5vw;"></div> | + | <div class ="row" style="height:5vw;"></div> |
<div class = "row"> | <div class = "row"> | ||
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<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="99%" style="width:99.54%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt; | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="99%" style="width:99.54%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt; | ||
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt"> | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt"> | ||
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18. | + | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.3pt"> |
| − | <td width=" | + | <td width="21%" style="width:21.4%;border:solid windowtext 1.0pt;mso-border-alt: |
| − | + | solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt; | |
| − | height:18. | + | height:18.3pt"> |
| − | <p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></b></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand">PART |
| − | <p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p> | + | NAME <span class="GramE">/ <span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></b></p> |
| + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></b></p> | ||
</td> | </td> | ||
| − | + | <td width="15%" style="width:15.3%;border:solid windowtext 1.0pt;border-left: | |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | <td width=" | + | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt"> |
| − | none;mso-border- | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand">TYPE<o:p></o:p></span></b></p> |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height: | + | |
| − | <p class="MsoNormal" align="center" style="text-align:center">< | + | |
| − | + | ||
| − | + | ||
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" style="width:15.6%;border:solid windowtext 1.0pt;border-left: |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | mso-border- | + | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt"> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand">SOURCE<o:p></o:p></span></b></p> | |
| − | + | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | |
</td> | </td> | ||
| − | <td width=" | + | <td width="47%" style="width:47.7%;border:solid windowtext 1.0pt;border-left: |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | mso-border- | + | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt"> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand">KEY | |
| − | + | FEATURES<o:p></o:p></span></b></p> | |
| − | <p class="MsoNormal" align="center" style="text-align:center">< | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:1;height:11.35pt"> |
| − | <td width=" | + | <td width="21%" style="width:21.4%;border:solid windowtext 1.0pt;border-top: |
none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
| − | padding:0cm 5.4pt 0cm 5.4pt;height: | + | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | tab-stops:14.2pt"><span class="SpellE"><span lang="EN-US" style="font-size:13.0pt; |
| + | font-family:Quicksand">YncM-Winsulin</span></span><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p></o:p></span></p> | ||
| + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p> | ||
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" style="width:15.3%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height: | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p> |
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" style="width:15.6%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height: | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | tab-stops:14.2pt"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Bacillus |
| + | subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p> | ||
| + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> | ||
</td> | </td> | ||
| − | <td width=" | + | <td width="47%" style="width:47.7%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height: | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; |
| − | + | tab-stops:14.2pt"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Novel | |
| − | insulin analogue (<span class="SpellE">Winsulin</span>) with a 12AA | + | short chain insulin analogue (<span class="SpellE">Winsulin</span>) with a 12AA |
| − | (QRGGGSGGGQRR)<o:p></o:p></span></p> | + | linker (QRGGGSGGGQRR)<o:p></o:p></span></p> |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; |
| − | + | tab-stops:14.2pt"><span class="SpellE"><span lang="EN-US" style="font-size:13.0pt; | |
| − | + | font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size: | |
| − | <p class=" | + | 13.0pt;font-family:Quicksand"> secretion tag to translocate protein into |
| − | + | media<o:p></o:p></span></p> | |
| − | Tag purification<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; |
| − | <p class=" | + | tab-stops:14.2pt"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">N-terminal |
| − | + | 6x His Tag purification<o:p></o:p></span></p> | |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; |
| − | + | tab-stops:14.2pt"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">4x | |
| − | cleavage site<o:p></o:p></span></p> | + | GSS flexible linker<o:p></o:p></span></p> |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; |
| − | + | tab-stops:14.2pt"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">TEV | |
| − | binding site<o:p></o:p></span></p> | + | protease cleavage site<o:p></o:p></span></p> |
| − | <p class="MsoNormal" style=" | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; |
| + | tab-stops:14.2pt"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Extended | ||
| + | ribosome binding site<o:p></o:p></span></p> | ||
| + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:2;height:114.0pt"> |
| − | <td width=" | + | <td width="21%" rowspan="2" style="width:21.4%;border:solid windowtext 1.0pt; |
border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
| − | padding:0cm 5.4pt 0cm 5.4pt;height: | + | padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000</a><o:p></o:p></span></p> |
| − | + | ||
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" rowspan="2" style="width:15.3%;border-top:none;border-left: |
none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height: | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p> |
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" style="width:15.6%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height: | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span> |
| − | + | Tag)<o:p></o:p></span></p> | |
</td> | </td> | ||
| − | <td width=" | + | <td width="47%" rowspan="2" style="width:47.7%;border-top:none;border-left: |
none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height: | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> |
| − | <p class="MsoNormal" style=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span> | |
| − | <p class=" | + | tag for targeting proteins to the periplasm<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">N terminal 6x His Tag<o:p></o:p></span></p> | |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p> | |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Extended ribosomal binding | |
| − | <p class=" | + | site<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> | |
| − | + | ||
| − | <p class=" | + | |
| − | + | ||
| − | + | ||
| − | <p class=" | + | |
| − | + | ||
| − | + | ||
| − | <p class=" | + | |
| − | + | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:3;height:114.0pt"> |
| − | <td width=" | + | <td width="15%" style="width:15.6%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height: | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span> |
proinsulin)<o:p></o:p></span></p> | proinsulin)<o:p></o:p></span></p> | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:114.0pt"> |
| − | <td width=" | + | <td width="21%" style="width:21.4%;border:solid windowtext 1.0pt;border-top: |
none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
| − | padding:0cm 5.4pt 0cm 5.4pt;height: | + | padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes"> </span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p> |
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" style="width:15.3%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height: | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p> |
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" style="width:15.6%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height: | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span> |
proinsulin)<o:p></o:p></span></p> | proinsulin)<o:p></o:p></span></p> | ||
</td> | </td> | ||
| − | <td width=" | + | <td width="47%" style="width:47.7%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height: | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; |
| − | + | tab-stops:14.2pt"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Human | |
| − | <p class=" | + | proinsulin<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; | |
| − | + | tab-stops:14.2pt"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">N-terminal | |
| − | <p class=" | + | 6x His tag<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; | |
| − | <p class=" | + | tab-stops:14.2pt"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">4x |
| − | + | GGS flexible linker<o:p></o:p></span></p> | |
| − | cleavage site for His-tag and C-peptide cleavage<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="margin-left:7.1pt;text-align:center; |
| − | <p class=" | + | tab-stops:14.2pt"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Trypsin |
| − | + | protease cleavage site for His-tag and C-peptide cleavage<o:p></o:p></span></p> | |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Extended ribosomal binding | |
| + | site<o:p></o:p></span></p> | ||
</td> | </td> | ||
</tr> | </tr> | ||
</tbody></table> | </tbody></table> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
| Line 395: | Line 376: | ||
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt"> | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt"> | ||
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.3pt"> | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.3pt"> | ||
| − | <td | + | <td width="21%" style="width:21.4%;border:solid windowtext 1.0pt;mso-border-alt: |
| − | + | solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt; | |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | height:18.3pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></b></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand">PART | |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | NAME <span class="GramE">/ <span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></b></p> |
| + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></b></p> | ||
</td> | </td> | ||
| − | + | <td width="15%" style="width:15.3%;border:solid windowtext 1.0pt;border-left: | |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | <td width=" | + | |
| − | none;mso-border- | + | |
background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt"> | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center">< | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand">TYPE<o:p></o:p></span></b></p> |
| − | + | ||
| − | + | ||
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" style="width:15.6%;border:solid windowtext 1.0pt;border-left: |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | mso-border- | + | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt"> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand">SOURCE<o:p></o:p></span></b></p> | |
| − | + | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | |
</td> | </td> | ||
| − | <td width=" | + | <td width="47%" style="width:47.7%;border:solid windowtext 1.0pt;border-left: |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | mso-border- | + | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt"> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand">KEY | |
| − | + | FEATURES<o:p></o:p></span></b></p> | |
| − | <p class="MsoNormal" align="center" style="text-align:center">< | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:1;height:11.35pt"> |
| − | <td width=" | + | <td width="21%" style="width:21.4%;border:solid windowtext 1.0pt;border-top: |
none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p> |
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" style="width:15.3%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p> |
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" style="width:15.6%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font- | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">N/A </span><span lang="EN-US" style="font-family: | |
| − | + | Helvetica;mso-fareast-font-family:Helvetica;mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-family:Quicksand"> <span class="SpellE">Winsulin</span> | |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | is <span class="SpellE">self designed</span> from literature and mammalian |
| + | insulins<o:p></o:p></span></p> | ||
| + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> | ||
</td> | </td> | ||
| − | <td width=" | + | <td width="47%" style="width:47.7%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | ||
| − | <p class=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Novel single chain insulin analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p> | |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p> | |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p> | |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> | |
| − | + | ||
| − | <p class=" | + | |
| − | + | ||
| − | + | ||
| − | <p class=" | + | |
| − | + | ||
| − | + | ||
| − | <p class=" | + | |
| − | + | ||
| − | + | ||
| − | <p class=" | + | |
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:2;height:114.0pt"> |
| − | <td width=" | + | <td width="21%" rowspan="2" style="width:21.4%;border:solid windowtext 1.0pt; |
border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-family:Quicksand">-Proinsulin<o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000</a><o:p></o:p></span></p> |
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" rowspan="2" style="width:15.3%;border-top:none;border-left: |
none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p> |
</td> | </td> | ||
| − | <td width=" | + | <td width="15%" style="width:15.6%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span> Tag)<o:p></o:p></span></p> |
| − | + | ||
| − | + | ||
</td> | </td> | ||
| − | <td width=" | + | <td width="47%" rowspan="2" style="width:47.7%;border-top:none;border-left: |
none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | ||
| − | <p class="MsoNormal"><span lang="EN-US" style=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Human proinsulin<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span> tag | |
| − | <p class=" | + | for targeting proteins to the periplasm<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">N terminal 6x His Tag<o:p></o:p></span></p> | |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p> | |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Extended ribosomal binding site<o:p></o:p></span></p> | |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> |
| − | + | ||
| − | + | ||
| − | <p class=" | + | |
| − | + | ||
| − | + | ||
| − | <p class=" | + | |
| − | + | ||
| − | + | ||
| − | <p class=" | + | |
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:3;mso-yfti-lastrow:yes;height:114.0pt"> |
| − | <td width=" | + | <td width="15%" style="width:15.6%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Human (Human proinsulin)<o:p></o:p></span></p> |
| − | + | ||
</td> | </td> | ||
</tr> | </tr> | ||
| Line 547: | Line 494: | ||
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt"> | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt"> | ||
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt"> | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt"> | ||
| − | <td width=" | + | <td width="21%" style="width:21.16%;border:solid windowtext 1.0pt;mso-border-alt: |
| − | + | solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt; | |
height:4.75pt"> | height:4.75pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; |
font-family:Quicksand"><o:p> </o:p></span></b></p> | font-family:Quicksand"><o:p> </o:p></span></b></p> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; |
| − | + | font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></b></p> | |
| − | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; |
font-family:Quicksand"><o:p> </o:p></span></b></p> | font-family:Quicksand"><o:p> </o:p></span></b></p> | ||
</td> | </td> | ||
| − | + | <td width="15%" style="width:15.14%;border:solid windowtext 1.0pt;border-left: | |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | <td width=" | + | |
| − | none;mso-border- | + | |
background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt"> | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
font-family:Quicksand">TYPE<o:p></o:p></span></b></p> | font-family:Quicksand">TYPE<o:p></o:p></span></b></p> | ||
</td> | </td> | ||
| − | <td width="15%" style="width:15. | + | <td width="15%" style="width:15.4%;border:solid windowtext 1.0pt;border-left: |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | mso-border- | + | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt"> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; | |
| − | + | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size: | + | |
| − | + | ||
| − | + | ||
font-family:Quicksand">SOURCE<o:p></o:p></span></b></p> | font-family:Quicksand">SOURCE<o:p></o:p></span></b></p> | ||
</td> | </td> | ||
| − | <td width=" | + | <td width="48%" style="width:48.3%;border:solid windowtext 1.0pt;border-left: |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | mso-border- | + | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt"> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; | |
| − | + | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size: | + | |
| − | + | ||
| − | + | ||
font-family:Quicksand">KEY FEATURES<o:p></o:p></span></b></p> | font-family:Quicksand">KEY FEATURES<o:p></o:p></span></b></p> | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:1;height:35.15pt"> |
| − | <td width="21%" rowspan="2" style="width:21. | + | <td width="21%" rowspan="2" style="width:21.16%;border:solid windowtext 1.0pt; |
border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt"> | padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p> |
</td> | </td> | ||
| − | <td width="15%" rowspan="2" style="width:15. | + | <td width="15%" rowspan="2" style="width:15.14%;border-top:none;border-left: |
none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p> |
</td> | </td> | ||
| − | <td width="15%" style="width:15. | + | <td width="15%" style="width:15.4%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span> | |
| − | <p class="MsoNormal" align="center" style="text-align:center | + | Tag)<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> | |
| − | + | ||
| − | <p class="MsoNormal" align="center" style="text-align:center | + | |
| − | + | ||
</td> | </td> | ||
| − | <td width=" | + | <td width="48%" rowspan="2" style="width:48.3%;border-top:none;border-left: |
none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt"> | ||
| − | <p class=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Novel single chain insulin |
| − | + | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker | |
| − | + | (QRGGGSGGGQRR)<o:p></o:p></span></p> | |
| − | (QRGGGSGGGQRR) | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span> |
| − | <p class=" | + | tag for targeting proteins to the periplasm<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p> | |
| − | <p class=" | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p> | |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p> | |
| − | <p class=" | + | |
| − | + | ||
| − | + | ||
| − | <p class=" | + | |
| − | + | ||
| − | + | ||
| − | <p class=" | + | |
| − | + | ||
| − | + | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt"> |
| − | <td width="15%" style="width:15. | + | <td width="15%" style="width:15.4%;border-top:none;border-left:none; |
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:13.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica; | |
| − | <p class="MsoNormal" align="center" style="text-align:center | + | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size: |
| − | + | 13.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p> | |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> | |
| − | + | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | |
</td> | </td> | ||
</tr> | </tr> | ||
Revision as of 13:44, 28 October 2017
GOLD
|
PART
NAME / NO. |
TYPE |
SOURCE |
KEY
FEATURES |
|
YncM-Winsulin |
COMPOSITE PART |
Bacillus
subtilis (YncM secretion tag) |
Novel
short chain insulin analogue (Winsulin) with a 12AA
linker (QRGGGSGGGQRR) YncM secretion tag to translocate protein into
media N-terminal
6x His Tag purification 4x
GSS flexible linker TEV
protease cleavage site Extended
ribosome binding site |
|
Ecotin-Proinsulin |
COMPOSITE PART |
E. Coli (Ecotin
Tag) |
Human proinsulin N-terminal Ecotin
tag for targeting proteins to the periplasm N terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosomal binding
site |
|
Human (Human
proinsulin) |
|||
|
Cytoplasmic-Proinsulin BBa_K2417001 |
COMPOSITE PART |
Human (Human
proinsulin) |
Human
proinsulin N-terminal
6x His tag 4x
GGS flexible linker Trypsin
protease cleavage site for His-tag and C-peptide cleavage Extended ribosomal binding
site |
SILVER
|
PART
NAME / NO. |
TYPE |
SOURCE |
KEY
FEATURES |
|
Cytoplasmic-Winsulin |
COMPOSITE PART |
N/A – Winsulin
is self designed from literature and mammalian
insulins |
Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR) N-terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosome binding site |
|
Ecotin-Proinsulin |
COMPOSITE PART |
E. Coli (Ecotin Tag) |
Human proinsulin N-terminal Ecotin tag
for targeting proteins to the periplasm N terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosomal binding site |
|
Human (Human proinsulin) |
BRONZE
|
PART NAME / NO. |
TYPE |
SOURCE |
KEY FEATURES |
|
Ecotin-Winsulin |
COMPOSITE PART |
E. Coli (Ecotin
Tag) |
Novel single chain insulin
analogue (Winsulin) with a 12AA linker
(QRGGGSGGGQRR) N-terminal Ecotin
tag for targeting proteins to the periplasm N-terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosome binding site |
|
N/A – Winsulin is self designed from literature and mammalian insulins |
