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− | <center><h2>Applied Design</h2>
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| <br> | | <br> |
| <h6>Contamination of aquatic and terrestrial environments with crude oils (or petroleum) represents a serious problem worldwide that results in both immediate and long-term environmental damages with toxicity to plants and/or soil invertebrates, as well as risks to humans and wildlife. Crude oils, while made of complex mixtures of thousands of types of molecular compounds, are predominantly composed of hydrocarbons including linear alkanes and aromatic hydrocarbons based on a 6-carbon ring account for approximately 80% of the total petroleum hydrocarbons in crude oils. Aromatic carbons such as polycyclic aromatic hydrocarbons (PAHs) like fluorene and phenanthrene are the molecular compounds in oil that are the most toxic as they can be quite persistent in the environment due to their intrinsic stability provided by the aromatic ring. | | <h6>Contamination of aquatic and terrestrial environments with crude oils (or petroleum) represents a serious problem worldwide that results in both immediate and long-term environmental damages with toxicity to plants and/or soil invertebrates, as well as risks to humans and wildlife. Crude oils, while made of complex mixtures of thousands of types of molecular compounds, are predominantly composed of hydrocarbons including linear alkanes and aromatic hydrocarbons based on a 6-carbon ring account for approximately 80% of the total petroleum hydrocarbons in crude oils. Aromatic carbons such as polycyclic aromatic hydrocarbons (PAHs) like fluorene and phenanthrene are the molecular compounds in oil that are the most toxic as they can be quite persistent in the environment due to their intrinsic stability provided by the aromatic ring. |
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| We have conducted a series of degradation experiments to measure the efficacy of the newly engineered strains containing optimized synthetic phenanthrene and fluorene degradation pathway, demonstrating the bacteria's ability to harness the PAHs as a carbon source and ultimately degrading the compounds. These bacteria can be incorporated in oil spill remediation and bioreactor use as commercial product, achieving detoxification through combinatorial genetic bioremediation. | | We have conducted a series of degradation experiments to measure the efficacy of the newly engineered strains containing optimized synthetic phenanthrene and fluorene degradation pathway, demonstrating the bacteria's ability to harness the PAHs as a carbon source and ultimately degrading the compounds. These bacteria can be incorporated in oil spill remediation and bioreactor use as commercial product, achieving detoxification through combinatorial genetic bioremediation. |
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