Difference between revisions of "Team:BostonU HW/Ligation"
| Line 80: | Line 80: | ||
</div> | </div> | ||
<div class="col-md-3" style="text-align:center; margin:auto; vertical-align:middle;" > | <div class="col-md-3" style="text-align:center; margin:auto; vertical-align:middle;" > | ||
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2017/2/28/MARS_Ligation_Protocol.pdf"download> |
<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button> | <button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button> | ||
</a> | </a> | ||
Revision as of 19:48, 29 October 2017
Summary
Ligation is a commonly used protocol in synthetic biology. In molecular cloning ligation is the process by which external DNA is inserted into a vector DNA, often a plasmid, using the enzyme DNA ligase. The newly formed DNA, or recombinant DNA, can then be analyzed or transformed.
This microfluidic chip is designed to perform ligation. T4 DNA Ligase Buffer, vector DNA, insert DNA, water, and T4 DNA Ligase are metered on the chip and are then mixed together. The solution then undergoes incubation, after which the recombinant DNA solution can pipetted out from the chip and used in further molecular cloning procedures
